Antiangiogenic Iridoids from Stachys ocymastrum and Premna resinosa

Planta Medica ◽  
2019 ◽  
Vol 85 (11/12) ◽  
pp. 1034-1039 ◽  
Author(s):  
Anna Maria Iannuzzi ◽  
César Muñoz Camero ◽  
Massimiliano DʼAmbola ◽  
Valeria DʼAngelo ◽  
Smain Amira ◽  
...  
Keyword(s):  
Chorioallantoic Membrane ◽  
Zebrafish Embryos ◽  
Iridoid Glucoside ◽  
Leaf Extracts ◽  
In Vivo Models ◽  
Antiangiogenic Activity ◽  
Chick Chorioallantoic Membrane ◽  
Vessel Growth

AbstractIn this paper, the isolation of one new iridoid glucoside, 6β-acetoxyipolamiide (1), and thirteen (2 – 14) known congeners from two Lamiaceae species, Stachys ocymastrum and Premna resinosa, leaf extracts is reported. The structural determination of the isolated compounds was performed by mono- and bidimensional NMR spectroscopic analysis as well as MS experiments. The isolates were assayed for their antiangiogenic activity by two in vivo models, zebrafish embryos and chick chorioallantoic membrane assays. The compounds with a significant antiangiogenic activity in both assays were β-hydroxyipolamiide (2), ipolamiide (3), and buddlejoside A5 (8). 6-O-α-l-(3″-O-p-Methoxycinnamoyl-4″-O-acetyl)rhamnopyranosyl catalpol (13) and 6-O-α-l-(2″-trans-caffeoyl)rhamnopyranosyl catalpol (6) showed the best antiangiogenic response on blood vessel growth in zebrafish embryos, whereas saccatoside (10) and 6-O-α-l-(2″-O - p-methoxycinnamoyl-3″-O-acetyl)rhamnopyranosyl catalpol (14) resulted in a strong reduction of capillary formation in the chorioallantoic membrane assay.

scholarly journals Study of antiangiogenic activity of “aqueous extract of Nigella sativa seeds” in chick chorioallantoic membrane (CAM) model

2018 ◽  
Vol 5 (4) ◽  
pp. 895
Author(s):  
Karunakar Kota ◽  
Sandhya Sharma ◽  
P. Ragavendhra
Keyword(s):  
Aqueous Extract ◽  
Nigella Sativa ◽  
Chorioallantoic Membrane ◽  
Dependent Manner ◽  
Antiangiogenic Effect ◽  
Antiangiogenic Activity ◽  
Chick Chorioallantoic Membrane ◽  
Chick Embryo Chorioallantoic Membrane ◽  
Vessel Growth

Background: Angiogenesis is important for the typical physiological activities such as cure from injury, menstrual cycle and embryo growth. It is also plays a crucial role in several pathological conditions in cancer. Antiangiogenesis, e.g., inhibition of blood vessel growth, is being investigated as a way to prevent the growth of tumors and other angiogenesis-dependent diseases. The chick embryo chorioallantoic membrane (CAM) is commonly used as an experimental in vivo assay to study both angiogenesis and antiangiogenesis in response to tissues, cells or soluble factors. Given the high occurrence of cancer worldwide and the major source of the discovery of new lead molecules are medicinal plants. The objective of the present research was to study the antiangiogenic property of “aqueous extract of Nigella sativa seeds” using chick chorioallantoic membrane (CAM) assayMethods: The chick chorioallantoic membrane (CAM) assay for screening the effect of Nigella sativa on anti-angiogenesis was performed according to the method given by Ribatti and co-workers.Results: The results of present study significantly increased the antiangiogenic effect on CAM by decreasing the proliferation of capillary networks in a dose (50 to 300 µg/egg) dependent manner which is probably related to the inhibition of neovascularization.Conclusions: It is concluded that aqueous extract of N. sativa seeds possesses significant antiangiogenic activity, and this is a possible rationale for its folkloric use as an anticancer agent.

scholarly journals Depletion of Embryonic Macrophages Leads to a Reduction in Angiogenesis in the Ex Ovo Chick Chorioallantoic Membrane Assay

Cells ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 5
Author(s):  
Hanna Tay ◽  
Charis Du Cheyne ◽  
Kristel Demeyere ◽  
Jurgen De Craene ◽  
Lobke De Bels ◽  
...  
Keyword(s):  
Chorioallantoic Membrane ◽  
Collagen Type I ◽  
Type I ◽  
Cam Assay ◽  
In Vivo Models ◽  
Chick Chorioallantoic Membrane ◽  
Angiogenesis Assay ◽  
Fold Reduction ◽  
Phosphate Buffered Saline

Macrophages play an important but poorly understood role in angiogenesis. To investigate their role in vessel formation, relevant in vivo models are crucial. Although the chick chorioallantoic membrane (CAM) model has been frequently used as an angiogenesis assay, limited data are available on the involvement of chicken macrophages in this process. Here, we describe a method to deplete macrophages in the ex ovo chick CAM assay by injection of clodronate liposomes and show that this depletion directly affects vascularisation of collagen onplants. Chicken embryos were injected intravenously with either clodronate or phosphate-buffered saline (PBS) liposomes, followed by placement of collagen type I plugs on the CAM to quantify angiogenic ingrowth. Clodronate liposome injection led to a significant 3.4-fold reduction of macrophages compared with control embryos as measured by immunohistochemistry and flow cytometry. Furthermore, analysis of vessel ingrowth into the collagen plugs revealed a significantly lower angiogenic response in macrophage-depleted embryos compared with control embryos, indicating that chicken embryonic macrophages play an essential function in the development of blood vessels. These results demonstrate that the chick CAM assay provides a promising model to investigate the role of macrophages in angiogenesis.

scholarly journals Antitumoural and antiangiogenic activity of Portuguese propolis in in vitro and in vivo models

2014 ◽  
Vol 11 ◽  
pp. 160-171 ◽  
Author(s):  
Ricardo Silva-Carvalho ◽  
Vera Miranda-Gonçalves ◽  
Ana Margarida Ferreira ◽  
Susana M. Cardoso ◽  
Abílio J.F.N. Sobral ◽  
...  
Keyword(s):  
In Vivo Models ◽  
Antiangiogenic Activity

The chick embryo chorioallantoic membrane model: a research approach for ex vivo and in vivo experiments

2021 ◽  
Vol 28 ◽  
Author(s):  
Ana Isabel Fraguas-Sánchez ◽  
Cristina Martín-Sabroso ◽  
Ana Isabel Torres-Suárez
Keyword(s):  
Animal Models ◽  
Chorioallantoic Membrane ◽  
New Drugs ◽  
Biological Research ◽  
Research Areas ◽  
Chick Chorioallantoic Membrane ◽  
Biodistribution Studies ◽  
Toxicological Studies ◽  
Cam Model

Background: The chick chorioallantoic membrane (CAM) model has attracted a great deal of interest in pharmaceutical and biological research as an alternative or complementary in vivo assay to animal models. Traditionally, CAM assay has been widely used to perform some toxicological studies, specifically to evaluate the skin, ocular and embryo toxicity of new drugs and formulations, and perform angiogenesis studies. Due to the possibility to generate the tumors onto the CAM, this model has also become an excellent strategy to evaluate the metastatic potential of different tumours and test the efficacy of novel anticancer therapies in vivo. Moreover, in the recent years, its use has considerably grown in other research areas, including the evaluation of new anti-infective agents, the development of biodistribution studies and tissue engineering research. Objectives: This manuscript provides a critical overview of the use of CAM model in pharmaceutical and biological research, especially to test the toxicity of new drugs and formulations and the biodistribution and the efficacy of novel anticancer and anti-infective therapies, analyzing its advantages and disadvantages compared to animal models. Conclusion: The chick chorioallantoic membrane model shows great utility in several research areas, such as cancer, toxicology, biodistribution studies and anti-infective therapies. In fact, it has become an intermediate stage between in vitro experiments and animal studies, and, in the case of toxicological studies (skin and ocular toxicity), has even replaced the animal models.

scholarly journals ELECTRON PROBE ANALYSIS OF THE CALCIUM DISTRIBUTION IN CELLS OF THE EMBRYONIC CHICK CHORIOALLANTOIC MEMBRANE I. A CRITICAL EVALUATION OF TECHNIQUES

1972 ◽  
Vol 20 (6) ◽  
pp. 401-413 ◽  
Author(s):  
JAMES R. COLEMAN ◽  
A. RAYMOND TEREPKA
Keyword(s):  
Electron Probe ◽  
Critical Evaluation ◽  
Chorioallantoic Membrane ◽  
Embryonic Chick ◽  
Total Calcium ◽  
Electron Probe Analysis ◽  
X Ray ◽  
Chick Chorioallantoic Membrane ◽  
Minor Losses

The chorioallantoic membrane of the developing chick embryo is an epithelium that actively transports calcium. The methodology utilized to prepare this soft tissue for calcium localization with the electron probe x-ray microanalyzer is presented in detail. The preparative procedures are evaluated according to general histochemical principles and in relationship specifically to electron probe investigations. It is shown that the method employed in these studies preserves the normal fine structure of the tissue, prevents selective loss of calcium, permits only minor losses of total calcium and appears to maintain the distribution of calcium that existed in vivo. Examples are presented of artifacts that can be induced during tissue sectioning and mounting procedures. Problems of defining electron probe resolution in biologic specimens are discussed, and the critical importance of evaluating x-ray images in association with simultaneously generated sample current images is emphasized.

Comparison of Two Different In Vivo Models and an In Vitro Model for Caloric Determination of Four Novel Fiber Ingredients

2013 ◽  
Vol 61 (50) ◽  
pp. 12374-12379 ◽  
Author(s):  
Sarah Cervantes-Pahm ◽  
Brenda K. Knapp ◽  
Beob G. Kim ◽  
Yanhong Liu ◽  
Carl M. Parsons ◽  
...  
Keyword(s):  
In Vitro Model ◽  
In Vivo Models ◽  
Vitro Model

scholarly journals Metabolite profiling and wound-healing activity of Boerhavia diffusa leaf extracts using in vitro and in vivo models

2020 ◽  
Vol 10 (1) ◽  
pp. 52-59 ◽  
Author(s):  
Kriti Juneja ◽  
Rutusmita Mishra ◽  
Samrat Chauhan ◽  
Sumeet Gupta ◽  
Partha Roy ◽  
...  
Keyword(s):  
Wound Healing ◽  
Metabolite Profiling ◽  
Leaf Extracts ◽  
In Vivo Models ◽  
Boerhavia Diffusa ◽  
Wound Healing Activity

Evaluation of Chemical, Antipsoriatic and Antiangiogenic Properties of Salt from Lonar Crater Lake Water

2019 ◽  
Vol 4 (3) ◽  
pp. 159-165
Author(s):  
Prakash Bansode ◽  
Indumathi Somasundaram ◽  
Apurva Birajdar ◽  
Sanjay Mishra ◽  
Dhanashree Patil ◽  
...  
Keyword(s):  
Lake Water ◽  
Crater Lake ◽  
Skin Diseases ◽  
Therapeutic Potential ◽  
Chorioallantoic Membrane ◽  
Soda Lake ◽  
Antiangiogenic Activity ◽  
The Impact ◽  
Lonar Crater

Lonar Crater lake was created by the impact of a massive meteor during the Pleistocene Epoch. Being a hypersaline and hyperalkaline soda lake, rich microbial diversity is reported earlier. Lonar lake water is used by local people and tribals against skin diseases. These observations prompted us to investigate the therapeutic potential of lake water against skin diseases. In this context, we have conducted pilot study to assess the antipsoriatic and antiangiogenic activity of the salt obtained from lake water using THP1 cell line by MTT assay and antiangiogenic activity by in vivo chicken chorioallantoic membrane (CAM) assay, as there is a close relation between psoriasis and angiogenesis. The results revealed that salt possess remarkable antipsoriatic and antiangiogenic activity.

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