Radical scavenging activity and phenolic components in different plant parts of Saraca asoca

The phenolic content and the radical scavenging activity were compared in leaves of sweetpotato (Ipomoea batatas L.) cultivars Shimon-1, Kyushu-119 and Elegant Summer grown under different temperature and shading conditions. Compared to cultivar differences, there was less effect of temperature and shading on the total phenolic content in sweetpotato leaves, however certain polyphenolic components differed widely among the treatments. The positive correlation between the radical scavenging activity and the level of total phenolics (r = 0.62) suggests that phenolic compounds are important antioxidant components of sweetpotato leaves. All the reverse-phase high-performance liquid chromatography (RP-HPLC) profiles of the cultivars tested showed peaks at the same retention times but peak areas of individual phenolic compounds differed with respective temperature and shading treatments. The phenolic compounds identified in the sweetpotato leaf were caffeic acid, chlorogenic acid, 4,5-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 3,4-di-O-caffeoylquinic acid, and 3,4,5-tri-O-caffeoylquinic acid. Most of the phenolic compounds were highest in leaves from plants grown at 20 °C without shading except 4,5-di-O-caffeoylquinic acid. The results indicate that growing leaves under moderately high temperatures and in full sun enhances the accumulation of phenolic components. These phenolic components have possible value in enhancing human health.

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Phenolic Compounds from the Aerial Parts of Adenophora triphylla (Thunb.) A. DC. var. triphylla and their Free Radical Scavenging Activity

Nepal Journal of Biotechnology ◽

10.3126/njb.v8i1.30205 ◽

2020 ◽

Vol 8 (1) ◽

pp. 12-16

Author(s):

Kengo Hori ◽

Hari Prasad Devkota

Keyword(s):

Free Radical ◽

Chlorogenic Acid ◽

Chemical Constituents ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Free Radical Scavenging Activity ◽

Scavenging Activity ◽

Aerial Parts ◽

Phenolic Components

Adenophora triphylla (Thunb.) A. DC. var. triphylla (Family: Campanulaceae) is distributed in Japan, Korea, and China. It is locally known as “Saiyousyajin” in Japan and the roots are used in traditional medicine to treat chronic bronchitis and whooping cough, and also as anti-inflammatory and anti-tussive agents. Till now, there is no report on the chemical constituents of aerial parts. Thus, the main aim of this study was to isolate and identify major chemical constituents of aerial parts of A. triphylla var. triphylla, and to evaluate their free radical scavenging activity. The 70% methanol extract of the aerial parts was subjected to repeated column chromatography using MCI gel CHP-20P, Sephadex LH-20, ODS and silica gel columns to isolate the five phenolic components (1-5). Free radical scavenging activity of the extract and compounds was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity method. The structures of the isolated compounds were elucidated as luteolin (1), luteolin 4’-O-b-glucopyranoside (2), luteolin 7-O-b-glucopyranoside (3), luteolin 7-O-neohesperidoside (4) and chlorogenic acid (5) based on their nuclear magnetic resonance (NMR) spectral data and comparison with literature values. All these compounds were isolated for the first time from A. triphylla var. triphylla. Extract showed weak free radical scavenging activity. Among isolated compounds, luteolin (1), luteolin 7-O-b-glucopyranoside (3), luteolin 7-O-neohesperidoside (4) and chlorogenic acid (5) showed potent free radical scavenging activity. Results from this study suggest that the aerial parts of A. triphylla var. triphylla might be a potential plant source for the development of functional foods, however further detailed research is necessary.

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The Effect of Different Drying Methods on the Phytochemicals and Radical Scavenging Activity of Ceylon Cinnamon (Cinnamomum zeylanicum) Plant Parts

European Journal of Medicinal Plants ◽

10.9734/ejmp/2014/11990 ◽

2014 ◽

Vol 4 (11) ◽

pp. 1324-1335 ◽

Cited By ~ 13

Author(s):

Darfour Bernard ◽

Asare Kwabena ◽

Ofosu Osei ◽

G. Daniel ◽

S. Elom ◽

...

Keyword(s):

Radical Scavenging Activity ◽

Radical Scavenging ◽

Drying Methods ◽

Scavenging Activity ◽

Cinnamomum Zeylanicum ◽

Plant Parts ◽

Ceylon Cinnamon

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Nonvolatile Chemical Constituents from the Leaves of Ligusticopsis wallichiana (DC.) Pimenov & Kljuykov and Their Free Radical-Scavenging Activity

Journal of Analytical Methods in Chemistry ◽

10.1155/2018/1794650 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 5

Author(s):

Hari Prasad Devkota ◽

Bibek Adhikari ◽

Takashi Watanabe ◽

Shoji Yahara

Keyword(s):

Free Radical ◽

Chemical Constituents ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Free Radical Scavenging Activity ◽

Scavenging Activity ◽

Traditional Medicines ◽

Plant Parts ◽

Major Chemical

Different plant parts of Ligusticopsis wallichiana (family: Apiaceae) are widely used as traditional medicines. Although many volatile constituents are already identified from the leaves of L. wallichiana, there is no detailed report on the nonvolatile constituents. In the present study, we aimed to isolate and identify the major chemical constituents from the leaves. Bhutkesoside A (1), falcarindiol (2), ferulic acid (3), cnidioside A (4), quercetin 3-O-β-D-glucopyranoside (5), rutin (6), 4′-O-methylquercetin 3-O-β-D-glucopyranoside (7), scopoletin (8), umbelliferone (9), eugenol 4-O-β-D-glucopyranoside (10) and pumilaside A (11) were isolated from the 70% MeOH extract. The structures of isolated compounds were elucidated on the basis of 1H- and 13C-NMR spectroscopic data. Compounds 4–11 are reported for the first time from L. wallichiana. Compounds 5 and 6 showed potent free radical-scavenging activity.

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Comparative Metabolomics Analysis and Radical Scavenging Activity of Saraca asoca (Roxb.) de Wilde Flowers in Different Stages of Maturity

Jordan Journal of Biological Sciences ◽

10.54319/jjbs/140317 ◽

2021 ◽

Vol 14 (03) ◽

pp. 513-522

Keyword(s):

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Saraca Asoca ◽

Comparative Metabolomics ◽

Metabolomics Analysis

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Phytochemical screening and biological activity of extracts of plant species Halacsya sendtneri (Boiss.) Dörfl.

Hemijska industrija ◽

10.2298/hemind110828068m ◽

2012 ◽

Vol 66 (1) ◽

pp. 43-51 ◽

Cited By ~ 5

Author(s):

Pavle Maskovic ◽

Nedeljko Manojlovic ◽

Anamarija Mandic ◽

Aleksandra Misan ◽

Ivan Milovanovic ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Ethyl Acetate ◽

Phenolic Compound ◽

Plant Species ◽

Ethyl Acetate Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Phenolic Components

This study is aimed at examining total polyphenol, flavonoid, gallotannin and condensed tannins content in acetone, chloroform, ethyl acetate and petroleum ether extracts of Halacsya sendtneri (Boiss.) D?rfl., their antimicrobial and antioxidant activities, as well as identifying and quantifying the phenolic components. The antioxidant activity is consistent with the results of total quantity of phenolic compound. The results showed that the acetone extract of plant species Halascya sendtneri (Boiss.) D?rfl. possessed the highest antioxidant activity. IC50 values were determined: 9.45?1.55 ?g/mL for DPPH free radical scavenging activity, 13.46?1.68 ?g/mL for inhibitory activity against lipid peroxidation, 59.11?0.83 ?g/mL for hydroxyl radical scavenging activity and 27.91?0.88 ?g/mL for ferrous ion chelating ability. The antimicrobial activity was tested using broth dilution procedure for determination of the minimum inhibitory concentration (MIC). The MICs were determined for 8 selected indicator strains. All of the extracts showed strong to moderate strong antimicrobial activity. The phenolic composition of Halacsya sendtneri extracts was determined by the HPLC method. The dominant phenolic compound in acetone, chloroform and ethyl acetate extract is rosmarinic acid. Ethyl acetate extract was also abundant in p-hydroxybenzoic acid and ferulic acid. The main compounds in petrol ether extract were chlorogenic acid and quercetin.

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EVALUATION OF TOTAL PHENOLIC, FLAVONOIDAL CONTENT, AND DPPH FREE RADICAL SCAVENGING ACTIVITY OF METHANOLIC EXTRACT OF AILANTHUS EXCELSA ROXB.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i4.16507 ◽

2017 ◽

Vol 10 (4) ◽

pp. 188

Author(s):

Aruna Kumari ◽

Sharma Ra

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Scavenging Activity ◽

Total Flavonoid Content ◽

Flavonoid Content ◽

Plant Parts ◽

Ailanthus Excelsa

Objective: Estimation of total phenol, flavonoid and antioxidant activity of various plant parts (leaves, stem, root, flower and fruit) of Ailanthus excelsa Roxb. methanolic extracts.Methods: Different plant parts were extracted with methanol, then the total phenol content was calculated by using Folin–Ciocalteu reagent, total flavonoid content was estimated by using Aluminum Chloride Colorimetric Method and the antioxidant activity was measured by DPPH (1,1-diphenyl-2-picrylhydrazyl radical) assay.Results: The highest total phenolic content (48.38 mg GAE/gdw) was observed in flower and lowest phenolic content in root (28.56 mg GAE/gdw). The highest total flavonoidal content (21.5 mg QE/gdw) was found in leaf and lowest in root (1.11 mg QE/gdw). The highest radical scavenging activity was found in flower extracts with the IC50 value of 36.85 µg/ml and the lowest scavenging activity was observed in root extract that was found to be 1493.46µg/ml.Conclusion: According to the results of present investigation the plant showed significant antioxidant activity that can be used for medical purpose for the treatment of various diseases.Keywords: Antioxidant activity, Ailanthus excelsa Roxb., Total phenolic content, Total flavonoid content, DPPH (2, 2- diphenyl- 1- picryl hydrazyl), Scavenging activity.

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