Oral Toxicity Studies, Histopathology and Anti-Diabetic activity of Polyherbal Extract in STZ induced diabetes in Rats

Background: Diabetes mellitus is a disease and endocrine disorder and it's a growing health problem in various countries. The prevalence of diabetes rises worldwide including South Africa 5.4% in 2025 increases as expected. The World Health Organization (WHO) estimated the diabetes mellitus problem in adults 173 million in developing counties. In this research observation of glucose levels indicated the diabetic state in Wistar rats by resulting from Streptozotocin administration and using a Metformin as a standard dose. This study demonstrated the acute oral toxicity and subacute oral toxicity of ethanolic extract of Saraca asoca leaves and Asparagus racemosus roots and showed the antidiabetic activity. Objective: To perform acute toxicity studies and sub-acute toxicity of the polyherbal ethanolic extract on the vital organ and isolated organ and record and noticed the visible changes on organs of each group of Wistar rats. Explore the hypoglycaemic action of the polyherbal extract of Saraca asoca and Asparagus racemosus. Methods: Wistar rats were divided into required groups for toxicity study first is acute oral toxicity 5,50, 300,2000 mg/kg body weight. Subacute oral toxicity studies were performed by administering a 250, 500, 1000mg/kg body weight. For demonstrating the antidiabetic activity the animals divided into 5 groups 1 normal control given saline group 2 standard dose Metformin compulsory dose groups 3 Streptozotocin-Induced diabetic 150mg/kg body weight body weight, groups 4 ethanolic extracts at a 100mg/kg groups 5 ethanolic extract 200mg/kg. On the last day of all the dosing period examined the Blood glucose levels and body weights of rat and histopathology studied were done by animal sacrifice and cut organs such as tissue pancreas, spleen, heart, lungs, liver, and kidney, placed on the slide and done a microscopic examination. Data selection has been complete by research papers from many databases such as NCBI, Web of science and Science direct and PubMed from year 1989 to 2020 by utilize research. skeywords such as “Antidiabetic”, “Saraca indica”, “Asparagus racemosus”, “ethanolic polyherbal extract”, “oral toxicity study”, “histopathology”, “Streptozotocin. Results : The polyherbal ethanolic extract of Saraca asoca and Asparagus racemosus at a dose of 100mg/kg and 200mg/kg was showed better effects against Streptozotocin-Induced diabetic 150mg/kg body weight body weight. All the extracts showed significantly (P <0.05) and it is safe and non-toxic nature by performed a toxicity study acute and subacute oral toxicity and the bodyweight are also improved, no inflammation and erosion are seen on any organs of Wistar rat by demonstrated a histopathology analysis. Conclusions: The polyherbal ethanolic extract of Saraca asoca and Asparagus racemosus showed hypoglycaemic activity against STZ-induced diabetes in experimental Wistar rats in Wistar rats. The results are shown beneficial effects of these ethanolic extract it helps in improving the changes in lipid metabolism, and protect the organs of Wistar rat liver, kidney, spleen, pancreas, lungs, heart against due to impairment of blood glucose and also in body weight. All organs were weighted and cut the tissue of organs and stained from eosin dye and changes observed by microscopy photos. no signs of inflammation and erosion.

Potential Use of Saraca Asoca in the Management of Artavadushti w.s.r. to Menstrual Disorders in Modern Era

Progress of a family depends on health of women in the family because she is the only creature who has amazing power of creation. A woman suffers from various menstrual disorders (Artavadushti) in her life span. Ashoka (Saraca indica or Saraca asoca (Roxb.), belonging to the family Caesalpinioideae, is one of the most important herb extensively used in bleeding disorders in women throughout her active reproductive period. Ashoka means no grief in Sanskrit language. So it is also called as “friend of women” because it has reputation of its bark for retaining a woman healthy and youthful. Chemically, it contains glycosides, flavonoids, tannins and saponins. Its actions are spasmogenic, uterotonic, oxytocic, antibacterial, antit-umour, anti-implantations, anti-progestational and anti-estrogenic to fight against menorrhagia, leucorrhoea and anticancer. In view of these properties and also its Rasa, Guna, Veerya, Vipaka it can be used in the treatment of bleeding gynaecological diseases.

PHARMACEUTICAL AND ANALYTICAL STUDY ON DIFFERENT PARTS OF ASHOKA [SARACA ASOCA ROXB. DE WILDE.]

Presently Ayurvedic medicines are becoming ultimate medicine rather than alternative medicine. The plant Asoka (Saraca asoca Roxb.)is one of the important plants of the Indian system of medicine known for its numerous pharmacological activities. After analysing the physicochemical and phytochemical properties of different parts of Ashoka, various formulations of different parts of Ashoka have been prepared. Hence, to establish the different parts of Ashoka as medicine, an attempt has been made to conduct a pharmaceutical and analytical study on various parts of Ashoka. In the present study, it can be conclusively said that the face pack of the root powder is beneficial in blackish discolouration of the face, root ointment in acne and blackish discolouration of the face, stem syrup is beneficial in eczema and acne, stem powder soap is useful in skin disease, bark Kashaya in gynaecological disorders, bark syrup in dysfunctional uterine bleeding and anaemia, bark granules in general debility and anaemia, tender leaves Tambuli in gastritis, leaf ointment in acne, flower Tambuli in gastritis and menorrhagia, flower syrup in gastritis and seed powder is useful in urinary calculi and burning micturition. The pharmaceutical evaluation of all the prepared products of Ashoka was done and analysed systematically. The multiple formulations of different parts of Ashoka (S. asoca) which are useful as food and medicine are the outcomes of the present study

Isolation of Natural Acid Base Indicator from the Flower Extract of Sarca Asoca, Abelmoschus Esulentus, Hibisucs Cannabinus And Hibiscus Rosa Sinensis

The objective of this work is to use more plant indicators than chemical based indicators. Saraca asoca is a species of family Fabaceae and Abelmoschus esculentus and Hibiscus cannabinus is a species of family Malvaceae [1]. Indicator are very special chemicals, they change colour of the solution with change in pH by adding acid or alkali. In the present work acid base titration has been performed by using natural indicators. The natural indicator is prepared from the most commonly occurring flower Hibiscus cannabinus, Abelmoschus esculentus Saraca asoca and Hibiscus Rosa sinensis. Aqueous and methanolic extract of flower were used as natural indicator. One acid one base were selected for acid base titration. Vinegar is used as acid and caustic soda is used as base. The results obtained by the natural indicator are almost similar to the result given by the synthetic indicators. Thus, natural indicator from flowers can be used for acid base titration [2]. Using aqueous extract of flower as indicator is more economical and with the same accuracy of result as that given by synthetic indicators.

Pharmacological Importance of Saraca asoca: A Review

Ayurveda is a traditional medicinal system which has been used consistently. Asoka is one of India's most holy and legendary trees. Ashoka, also known as Saraca asoca (Roxb.) Wilde, is an indigenous plant that belongs to the Caesalpiniaceae subfamily of the Legume. It is an evergreen tree of medium size. The objective of this review is to provide detailed description of botany, phytochemicals, medicinal value and pharmacological activity. Saraca asoca was reported to be comprising phytoconstituents, such as Flavonoids, Steroids, Glycosides, Saponins, Carbohydrates, Proteins as well as many Pharmacologic activities such as anti-diabetic, CNS depressant, anti-helminthic, cardio protective, anti-bacterial, anti-menorrhagic, anti-hyperglycaemic and anti-oxidant activities, anti-cancer activities.