Protective effects of Brazilian native fruits against reactive oxygen species

Background Mitochondrial changes that characterize the heart after anesthetic preconditioning (APC) or the mechanisms by which mitochondrial triggering factors lead to protection are unknown. This study hypothesized that generation of reactive oxygen species (ROS) during APC is required to initiate the mitochondrial protective effects, and that APC leads to improved mitochondrial electron transport chain function and cardiac function during reperfusion. Methods Isolated guinea pig hearts were subject to 30 min ischemia and 120 min reperfusion. Prior to ischemia hearts were either untreated (I/R), or treated with sevoflurane (APC), in the presence or absence of the ROS scavenger tiron (TIR), or the superoxide dismutase mimetic MnTBAP (TBAP). Intracellular ROS were measured by spectrofluorometry using the fluorescent probe dihydroethidium (DHE). In another series of experiments, using the same protocol, hearts were reperfused for only 5 min and removed for measurement of adenosine triphosphate (ATP) synthesis by luciferin-luciferase luminometry and ROS generation by dichlorohydro-fluorescein (DCF) fluorescence in isolated mitochondria. Results The APC improved cardiac function and reduced infarction. Tiron or MnTBAP abrogated the protection afforded by APC. Mitochondrial ATP synthesis was decreased by 70 +/- 3% after IR alone, by only 7 +/- 3% after APC, by 69 +/- 2% after APC+TIR, and by 71 +/- 3% after APC + TBAP. Mitochondrial ROS formation (DCF) increased by 48 +/- 3% after IR alone, by 0 +/- 2% after APC, by 43 +/- 4% after APC + TIR, and by 46 +/- 3% after APC + TBAP. ROS generation (DHE) was increased in I/R group at 5 and 120 min reperfusion. This was attenuated by APC but this protective effect was abrogated in APC + TIR and APC + TBAP groups. Conclusions The results indicate that ROS are central both in triggering and mediating APC, and that the mitochondrion is the target for these changes.

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Protective effects of myricitrin against osteoporosis via reducing reactive oxygen species and bone-resorbing cytokines

Toxicology and Applied Pharmacology ◽

10.1016/j.taap.2014.08.004 ◽

2014 ◽

Vol 280 (3) ◽

pp. 550-560 ◽

Cited By ~ 15

Author(s):

Qiang Huang ◽

Bo Gao ◽

Long Wang ◽

Ya-Qian Hu ◽

Wei-Guang Lu ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Reactive Oxygen ◽

Oxygen Species ◽

Protective Effects

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Protective effects of kaempferol against reactive oxygen species-induced hemolysis and its antiproliferative activity on human cancer cells

European Journal of Medicinal Chemistry ◽

10.1016/j.ejmech.2016.02.045 ◽

2016 ◽

Vol 114 ◽

pp. 24-32 ◽

Cited By ~ 56

Author(s):

Wenzhen Liao ◽

Luying Chen ◽

Xiang Ma ◽

Rui Jiao ◽

Xiaofeng Li ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Cancer Cells ◽

Antiproliferative Activity ◽

Human Cancer ◽

Reactive Oxygen ◽

Oxygen Species ◽

Protective Effects ◽

Human Cancer Cells

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Treatment of Rats With Hypolipidemic Compound Pirinixic Acid Protects Their Hearts Against Ischemic Injury: Are Mitochondrial KATP Channels and Reactive Oxygen Species Involved?

Physiological Research ◽

10.33549/physiolres.932591 ◽

2013 ◽

pp. 577-584 ◽

Cited By ~ 2

Author(s):

M. NEMČEKOVÁ ◽

S. ČARNICKÁ ◽

M. FERKO ◽

M. MURÁRIKOVÁ ◽

V. LEDVÉNYIOVÁ ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Rat Heart ◽

Ischemia Reperfusion ◽

Reactive Oxygen ◽

Katp Channels ◽

Ros Generation ◽

Peroxisome Proliferator ◽

Oxygen Species ◽

Protective Effects ◽

Mitochondrial Katp Channels

Hypolipidemic compound pirinixic acid (WY-14643, WY) is known to exert pleiotropic (other than primary) effects, such as activation of peroxisome proliferator-activated receptors (PPAR-α), transcription factors regulating different cardiac functions. Their role in ischemia-reperfusion (I/R) injury and cardioprotection is less clear, although protective effects of PPAR agonists have been documented. This study was designed to explore the effects of WY on the I/R injury in the rat heart and potential mechanisms involved, including mitochondrial KATP channels (mitoKATP) opening and production of reactive oxygen species (ROS). Langendorff-perfused hearts of rats intragastrally treated with WY (3 mg/kg/day) for 5 days and of control animals were subjected to 30-min global ischemia and 2-h reperfusion with or without 15-min perfusion with mitoKATP blocker 5-hydroxydecanoate (5-HD) prior to I/R. Evaluation of the infarct size (IS, TTC staining) served as the main end-point of protection. Lipid peroxidation (a marker of ROS production) was determined by measurement of myocardial concentration of conjugated dienes (CD), whereas protein expression of endothelial NO synthase was analysed by Western blotting. A 2-fold increase in the cardiac protein levels of eNOS after treatment with WY was accompanied by lower post-I/R levels of CD compared with those in the hearts of untreated controls, although WY itself enhanced ROS generation prior to ischemia. IS was reduced by 47 % in the hearts of WY-treated rats (P<0.05), and this effect was reversed by 5-HD. Results suggest that PPAR-α activation may confer protection against lethal I/R injury in the rat heart that involves up-regulation of eNOS, mitoKATP opening and reduced oxidative stress during I/R.

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A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles

Antioxidants ◽

10.3390/antiox10111727 ◽

2021 ◽

Vol 10 (11) ◽

pp. 1727

Author(s):

Sang-Cheol Lee ◽

Na-Hyun Lee ◽

Kapil D. Patel ◽

Soo-Kyung Jun ◽

Jeong-Hui Park ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Skeletal Muscle ◽

Contractile Activity ◽

Reactive Oxygen ◽

Selenium Nanoparticles ◽

C2c12 Cells ◽

Myoblast Differentiation ◽

Oxygen Species ◽

Protective Effects ◽

Intracellular Ros

Reactive oxygen species (ROS) are continuously produced by skeletal muscle during contractile activity and even at rest. However, the ROS generated from excessive exercise or traumatic damage may produce more ROS than can be neutralized by an antioxidant capacity, which can be harmful to muscle function. In particular, selenium is a known antioxidant that regulates physiological functions such as cell differentiation and anti-inflammatory function. In this study, we developed nano-sized antioxidative biomaterials using selenium to investigate the protective and differentiation effects against C2C12 myoblasts in an H2O2-induced oxidative stress environment. The selenium nanoparticles (SeNPs) were produced with a size of 35.6 ± 4.3 nm and showed antioxidant effects according to the 3,3′,5,5′-tetramethylbenzidine assay. Then, SeNPs were treated to C2C12 cells with or without H2O2. Our results showed that SeNPs reduced C2C12 apoptosis and intracellular ROS levels. Additionally, SeNPs effectively up-regulated in the presence of H2O2, MyoD, MyoG, α-actinin, and myosin heavy chain, which are well known to increase during myoblast differentiation as assayed by qRT-PCR, immunocytochemistry-staining, western blotting. These results demonstrate that SeNPs can accelerate differentiation with its protective effects from the ROS environment and can be applied to the treatment of skeletal muscle in a cellular redox environment.

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Protective effects of hesperetin on the quality of sperm, apoptosis, lipid peroxidation, and oxidative stress during the process of cryopreservation: An experimental study

International Journal of Reproductive BioMedicine ◽

10.18502/ijrm.v19i1.8178 ◽

2021 ◽

Author(s):

Jamal Valipour ◽

Sina Mojaverrostami ◽

Beheshteh Abouhamzeh ◽

Masoumeh Abdollahi

Keyword(s):

Reactive Oxygen Species ◽

Lipid Peroxidation ◽

Experimental Study ◽

Reactive Oxygen ◽

Human Sperm ◽

Control Group ◽

Oxygen Species ◽

Protective Effects ◽

Beneficial Effects

Background: Hesperetin is a bioflavonoid compound, largely used in Chinese traditional medicine and found plenty in citrus fruits. Hesperetin has beneficial effects against different diseases. The sperm cryopreservation process is a common method that is used in infertility laboratories. It has been reported that during the cryopreservation process, the quality of sperm is significantly reduced. Objective: To investigate the effect of hesperetin on the quality of human spermatozoa during the cryopreservation process. Materials and Methods: In this experimental study, 22 sperm sample of normozoospermia men who referred to the infertility department of the Shariati Hospital (Tehran, Iran) Between October and November 2019 were collect and divided into three groups as: 1) fresh, 2) control (frozen-thawed group without treatment), and 3) treatment group as frozen-thawed samples supplemented with 20 μM hesperetin. Motility, Viability, morphology, Apoptotic-like changes, intracellular H2O2, intracellular O2−, and lipid peroxidation (LPO) was measured. Results: Hesperetin treatment during the cryopreservation process of human sperm significantly improved the viability, motility, and morphology rates of the spermatozoa after frozen-thawed process in control group (p < 0.01). In addition, it significantly reduced the reactive oxygen species (ROS) level, LPO level and increased the percentage of viable sperm cells with intact plasma membrane (p < 0.01) after frozen-thawed process. Conclusion: Hesperetin can improve the quality of human sperm and also protect human sperm against reactive oxygen species, LPO, and apoptosis during the cryopreservation-thawing process. Key words: Cryopreservation, Hesperetin, Spermatozoa, Reactive oxygen species.

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