scholarly journals Protective effects of hesperetin on the quality of sperm, apoptosis, lipid peroxidation, and oxidative stress during the process of cryopreservation: An experimental study

2021 ◽  
Author(s):  
Jamal Valipour ◽  
Sina Mojaverrostami ◽  
Beheshteh Abouhamzeh ◽  
Masoumeh Abdollahi
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Experimental Study ◽  
Reactive Oxygen ◽  
Human Sperm ◽  
Control Group ◽  
Oxygen Species ◽  
Protective Effects ◽  
Beneficial Effects

Background: Hesperetin is a bioflavonoid compound, largely used in Chinese traditional medicine and found plenty in citrus fruits. Hesperetin has beneficial effects against different diseases. The sperm cryopreservation process is a common method that is used in infertility laboratories. It has been reported that during the cryopreservation process, the quality of sperm is significantly reduced. Objective: To investigate the effect of hesperetin on the quality of human spermatozoa during the cryopreservation process. Materials and Methods: In this experimental study, 22 sperm sample of normozoospermia men who referred to the infertility department of the Shariati Hospital (Tehran, Iran) Between October and November 2019 were collect and divided into three groups as: 1) fresh, 2) control (frozen-thawed group without treatment), and 3) treatment group as frozen-thawed samples supplemented with 20 μM hesperetin. Motility, Viability, morphology, Apoptotic-like changes, intracellular H2O2, intracellular O2−, and lipid peroxidation (LPO) was measured. Results: Hesperetin treatment during the cryopreservation process of human sperm significantly improved the viability, motility, and morphology rates of the spermatozoa after frozen-thawed process in control group (p < 0.01). In addition, it significantly reduced the reactive oxygen species (ROS) level, LPO level and increased the percentage of viable sperm cells with intact plasma membrane (p < 0.01) after frozen-thawed process. Conclusion: Hesperetin can improve the quality of human sperm and also protect human sperm against reactive oxygen species, LPO, and apoptosis during the cryopreservation-thawing process. Key words: Cryopreservation, Hesperetin, Spermatozoa, Reactive oxygen species.

scholarly journals Antioxidant effect of Mn2+ on capacitation and acrosome reaction of fresh and chilled cattle bull semen

2012 ◽  
Vol 1 (1) ◽  
pp. 18
Author(s):  
Amrit Kaur Bansal ◽  
Ranjna Sundhey Cheema ◽  
Vinod Kumar Gandotra
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Acrosome Reaction ◽  
Reactive Oxygen ◽  
Antioxidant Effect ◽  
Oxygen Species ◽  
Sperm Capacitation ◽  
Bull Semen

The aim of this paper was to investigate the antioxidant effect of Mn2+ (200 mM) on the sperm capacitation and acrosome reaction of fresh and chilled cattle bull semen. It has been found that Mn2+ supplementation improves (P≤0.05) the motility at 0, 2, 4 and 6 h of incubation. MDA (malondialdehyde), end product of lipid peroxidation, decreases significantly (P≤0.05) with the supplementation of manganese at 0- and 6-hr of incubation both in fresh and chilled semen. Manganese also increases acrosome reaction significantly (P≤0.05) both in fresh and chilled semen at 0, 4 and 6 h of incubation. Therefore, our findings suggest the role of Mn2+supplementation in improving the quality of cattle bull semen by its scavenging property<em> i.e.</em> reduction in the production of reactive oxygen species during its storage at 4°C or incubation at 37°C for capacitation.

scholarly journals Pengaruh pemberian ekstrak jintan hitam (Nigella sativa) terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) yang terpapar asap rokok

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Irene M. A. Rumampuk ◽  
Lydia Tendean ◽  
Grace L. A. Turalaki
Keyword(s):  
Reactive Oxygen Species ◽  
Body Weight ◽  
Cigarette Smoke ◽  
Nigella Sativa ◽  
Sperm Quality ◽  
Reactive Oxygen ◽  
Control Group ◽  
Continuous Exposure ◽  
Oxygen Species

Abstract: Continuous exposure to cigarette smoke has been reported as the number one cause of infertility in men. Cigarette smoke increases the production of reactive oxygen species (ROS) thereby causing infertility in men. Nigella sativa contains antioxidant that may decrease ROS level in hope to improve infertility. This 50 days study is conducted to see the effect of Nigella sativa extract to the sperm quality of nine wistar rats that have been exposed by cigarette smoke. The rats were divided into three groups of three: the control group and the group treated with Nigella sativa administered orally at the dose of 200 mg kg body weight and 400 mg/kg body weight. Sperm quality was observed by overlooking at the concentration, motility, and morphology of each group. The mean data of each group was obtained and tested for significance using Independent Samples T-Test. This study found the comparison of both motility (p=0,006) and morphology (p=0,000) between control group and the group with the dose of 200 mg/kg body weight to be significant, as well as the comparison of motility (p=0,001) and morphology (p=0,000) between the control group and the group with the dose of 400 mg/kg body weight.Keywords: sperm quality, nigella sativa, smoking, cigaretteAbstrak: Paparan secara terus menerus terhadap asap rokok merupakan penyebab infertilitas utama pada pria. Asap rokok dapat meningkatkan Reactive oxygen species (ROS) yang selanjutnya dapat menyebabkan infertilitas. Nigella sativa mengandung antioksidan yang dapat menunrunkan jumlah ROS dan diharapkan untuk dapat mengkoreksi infertilitas. Penelitian yang dilaksanakan selama 50 hari ini bertujuan untuk melihat pengaruh dari ekstrak Nigella sativa terhadap kualitas spermatozoa sembilan tikus wistar yang terpapar asap rokok. Tikus dibagi menjadi tiga kelompok masing-masing terdiri atas tiga tikus; terdapat kelompok kontrol dan kelompok yang diberi ekstrak Nigella sativa dengan dosis 200 mg/kg BB dan 400 mg/kg BB. Kualitas spermatozoa dinilai berdasarkan konsentrasi, motilitas dan morfologi dari masing-masing kelompok perlakuan. Data rerata dari tiap kelompok perlakuan diambil dan di diuji signifikans menggunakan uji T-independen. Penelitian ini mendapati perbandingan baik motilitas (p=0,006) ataupun morfologi (p=0,000) dari kelompok kontrol dan perlakuan dengan dosis 200 mg/kg BB dibilang signifikan, sama halnya dengan perbandingan antara motilitas (0,001) dan morfologi (0,000) dari kelompok kontrol dan perlakuan dengan dosis 400 mg/kg BB.Kata kunci: kualitas sperma, jintan hitam, rokok

scholarly journals Pengaruh pemberian vitamin C terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) setelah pemaparan cahaya

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Risza Intan Suryani ◽  
Lusiana Satiawati ◽  
Janette M. Rumbajan
Keyword(s):  
Reactive Oxygen Species ◽  
Free Radical ◽  
Treatment Group ◽  
Vitamin C ◽  
Rattus Norvegicus ◽  
Reactive Oxygen ◽  
Male Rats ◽  
Control Group ◽  
Oxygen Species

Abstract: Light is an initiator of free radical oxygen formation inter alia reactive oxygen species (ROS) that increases the oxidative stress. Free radicals can be controlled and prevents by the antioxidant. Vitamin C is an antioxidant that can neutralize the free radical. This study aimed to obtain the effects of vitamin C on spermatozoa quality of wistar rats after light exposure. Subjects were male rats (Rattus norvegicus) which were exposed to light and then divided into three groups: control group (without vitamin C administration), treatment group 1 (treated with vitamin C 1.8 mg/day/rat), and treatment group 2 (treated with of vitamin C 3.6 mg/day/rat) for 50 days. The results showed a significant improvement in the quality of wistar spermatozoa (concentration, motility, and morphology) after treated with vitamin C and exposed to light.Keywords: vitamin C, light, quality of spermatozoa, ROS (reactive oxygen species).  Abstrak: Cahaya merupakan inisiator pembentukan radikal bebas reactive oxygen species (ROS) yang akan meningkatkan stres oksidatif. Radikal bebas dapat dikendalikan dan dicegah oleh antioksidan, salah satunya ialah vitamin C. Penelitian ini bertujuan untuk mengetahui efek pemberian vitamin C terhadap kualitas spermatozoa tikus wistar setelah pemaparan cahaya. Subyek penelitian ialah tikus wistar jantan (Rattus norvegicus) yang diberi paparan cahaya kemudian dibagi menjadi 3 kelompok, yaitu: kelompok kontrol (tanpa pemberian vitamin C), kelompok perlakuan 1 (pemberian vitamin C 1,8 mg/hari/ekor), dan kelompok perlakuan 2 (pemberian vitamin C 3,6 mg/hari/ekor). Hasil penelitian memperlihatkan peningkatan kualitas spermatozoa tikus wistar (Rattus norvegicus) secara bermakna setelah pemberian vitamin C pada tikus wistar (Rattus norvegicus) yang telah diberi pemaparan cahaya.Kata kunci: vitamin C, cahaya, kualitas spermatozoa, ROS

Protective effects of Brazilian native fruits against reactive oxygen species

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
J Infante ◽  
A Massarioli ◽  
PL Rosalen ◽  
S Alencar
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Protective Effects

Altered composition of high-lipid diet may generate reactive oxygen species by disturbing the balance of antioxidant and free radicals

2020 ◽  
Vol 31 (3) ◽  
Author(s):  
Arnab Banerjee ◽  
Debasmita Das ◽  
Rajarshi Paul ◽  
Sandipan Roy ◽  
Ankita Bhattacharjee ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Risk Factor ◽  
Reactive Oxygen ◽  
Control Group ◽  
Preliminary Evaluation ◽  
Oxygen Species ◽  
High Lipid ◽  
Tnf Α ◽  
High Lipid Diet ◽  
Lipid Diet

AbstractBackgroundIn the present era, obesity is increasing rapidly, and high dietary intake of lipid could be a noteworthy risk factor for the occasion of obesity, as well as nonalcoholic fatty liver disease, which is the independent risk factor for type 2 diabetes and cardiovascular disease. For a long time, high-lipid diet (HLD) in “fast food” is turning into part of our everyday life. So, we were interested in fulfilling the paucity of studies by means of preliminary evaluation of these three alternative doses of HLD on a rat model and elucidating the possible mechanism of these effects and divulging the most alarming dose.MethodsThirty-two rats were taken, and of these, 24 were fed with HLD in three distinctive compositions of edible coconut oil and vanaspati ghee in a ratio of 2:3, 3:2 and 1:1 (n = 8), orally through gavage at a dose of 10 mL/kg body weight for a period of 28 days, whereas the other eight were selected to comprise the control group.ResultsAfter completion of the experiment, followed by analysis of data it was revealed that hyperlipidemia with increased liver and cardiac marker enzymes, are associated with hepatocellular injury and cardiac damage. The data also supported increased proinflammatory cytokines such as interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α). As oxidative stress parameter increased in both liver and heart, there is also an increased in TNF-α due to an increased expression of inducible nitric oxide (NO) synthase, which led to a high production of NO. Moreover, HLD treatment explicitly weakens reasonability of hepatocytes and cardiomyocytes conceivably through G0/G1 or S stage capture or perhaps by means of enlistment of sub-G0/G1 DNA fragmentation and a sign of apoptosis.ConclusionsBased on the outcomes, it tends to be inferred that consequences of the present examination uncovered HLD in combination of 2:3 applies most encouraging systemic damage by reactive oxygen species generation and hyperlipidemia and necroapoptosis of the liver and heart. Hence, outcome of this study may help to formulate health care strategy and warns about the food habit in universal population regarding the use of hydrogenated and saturated fats (vanaspati ghee) in diet.

scholarly journals Defining the Mechanisms by Which the Reactive Oxygen Species By-Product, 4-Hydroxynonenal, Affects Human Sperm Cell Function1

2015 ◽  
Vol 92 (4) ◽  
Author(s):  
Mark A. Baker ◽  
Anita Weinberg ◽  
Louise Hetherington ◽  
Ana-Izabel Villaverde ◽  
Tony Velkov ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Sperm Cell ◽  
Reactive Oxygen ◽  
Human Sperm ◽  
Oxygen Species

scholarly journals Ginkgo biloba extract (EGb 761) attenuates oxidative stress induction in erythrocytes of sickle cell disease patients

2012 ◽  
Vol 48 (4) ◽  
pp. 659-665 ◽  
Author(s):  
Aline Emmer Ferreira Furman ◽  
Railson Henneberg ◽  
Priscila Bacarin Hermann ◽  
Maria Suely Soares Leonart ◽  
Aguinaldo José do Nascimento
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Sickle Cell ◽  
Reduced Glutathione ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
Egb 761 ◽  
Cell Disease

Sickle cell disease promotes hemolytic anemia and occlusion of small blood vessels due to the presence of high concentrations of hemoglobin S, resulting in increased production of reactive oxygen species and decreased antioxidant defense capacity. The aim of this study was to evaluate the protective action of a standardized extract of Ginkgo biloba (EGb 761), selected due to its high content of flavonoids and terpenoids, in erythrocytes of patients with sickle cell anemia (HbSS, SS erythrocytes) subjected to oxidative stress using tert-butylhydroperoxide or 2,2-azobis-(amidinepropane)-dihydrochloride, in vitro. Hemolysis indexes, reduced glutathione, methemoglobin concentrations, lipid peroxidation, and intracellular reactive oxygen species were determined. SS erythrocytes displayed increased rates of oxidation of hemoglobin and membrane lipid peroxidation compared to normal erythrocytes (HbAA, AA erythrocytes), and the concentration of EGb 761 necessary to achieve the same antioxidant effect in SS erythrocytes was at least two times higher than in normal ones, inhibiting the formation of intracellular reactive oxygen species (IC50 of 13.6 µg/mL), partially preventing lipid peroxidation (IC50 of 242.5 µg/mL) and preventing hemolysis (IC50 of 10.5 µg/mL). Thus, EGb 761 has a beneficial effect on the oxidative status of SS erythrocytes. Moreover, EGb 761 failed to prevent oxidation of hemoglobin and reduced glutathione at the concentrations examined.

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