ANTI-ESTROGENIC PROPERTIES OF ETHANOLIC LEAF EXTRACT OF BOERHAVIA DIFFUSA ON FEMALE WISTAR RATS.

2017 ◽  
Author(s):  
C Olajide Omolola ◽  
J Okpuzor ◽  
I Nnadozie Jane ◽  
R Adewole Adeniyi
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Female Wistar Rats ◽  
Estrogenic Properties ◽  
Ethanolic Leaf Extract ◽  
Boerhavia Diffusa

scholarly journals Ameliorative potentials of Boerhavia diffusa Ethanolic leaf extract on cypermethrin-induced reproductive dysfunction in Wistar rats

2021 ◽  
Vol 1 (2) ◽  
pp. 038-044 ◽  
Author(s):  
Omoirri M. A. ◽  
Ehebha S. E. ◽  
Madubogwu N. U. ◽  
Gbagbeke K. O. ◽  
Uyovwiesevwa A. J.
Keyword(s):  
Blood Cell ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Health Indicators ◽  
Reproductive Dysfunction ◽  
Group I ◽  
Hematological Analysis ◽  
Animal House ◽  
Ethanolic Leaf Extract ◽  
Boerhavia Diffusa

With rising global reports on reproductive and hematological health indicators, numerous animal based researches have more often focused on herbal-based medical remedies. In this study, the ameliorative potentials of ethanolic leaf extract of Boerhavia diffusa was investigated on reproductive and selected hematological variables in wistar rats [packed cell volume (PCV), total white blood cell count (TWBCC), total red blood cell (TRBCC) count and total Platelet count (TPC)]. Twenty five (25) female wistar rats (between 150-200 g) were procured and acclimatized for two weeks in the animal house of Ambrose Alli University, Ekpoma, Edo State. The rats were then grouped into five (5) groups of five rats each (n = 5). While group I animals received standard rat chow and water at liberty (control), reproductive dysfunction was orally induced in group II rats, using 3.83 mg/kg bw of cypermethrin for 14 days. Groups III, IV and V received (orally) 200 mg/kg bw, 300 mg/kg bw and 400 mg/kg bw of Boerhavia diffusa extract (ethanolic) for five weeks respectively; haven also induced reproductive dysfunction. At the end of the period of administration, animals were euthanized under chloroform anaesthesia and blood sample obtained for hematological analysis, while also harvesting and observing the ovaries for histo-architectural changes. Results from one way analysis of variance (ANOVA) showed a statistically significant (p < 0.05) dose-dependent increase in the PCV TPC and TRBCC levels of all groups compared to control, while TWBCC increased insignificantly across groups. More studies to corroborative the observations from this works highly recommended.

scholarly journals Antidiarrheal Activity of Dissotis multiflora (Sm) Triana (Melastomataceae) Leaf Extract in Wistar Rats and Subacute Toxicity Evaluation

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Alian Désiré Afagnigni ◽  
Maximilienne Ascension Nyegue ◽  
Chantal Florentine Ndoye Foe ◽  
Youchahou Njankouo Ndam ◽  
Frédéric Nico Njayou ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Shigella Flexneri ◽  
Female Rats ◽  
Male Rats ◽  
Dependent Manner ◽  
Subacute Toxicity ◽  
Antidiarrheal Activity ◽  
Dose Dependent ◽  
Ethanolic Leaf Extract

The present work was undertaken to evaluate antidiarrheal activity of ethanolic leaf extract of Dissotis multiflora (Sm) Triana (D. multiflora) on Shigella flexneri-induced diarrhea in Wistar rats and its subacute toxicity. Diarrhea was induced by oral administration of 1.2 × 109 cells/mL S. flexneri to rats. Antidiarrheal activity was investigated in rats with the doses of 111.42 mg/kg, 222.84 mg/kg, and 445.68 mg/kg. The level of biochemical parameters was assessed and organs histology examined by 14 days’ subacute toxicity. S. flexneri stool load decreased significantly in dose-dependent manner. The level of ALT increased (p<0.05) in male rats treated with the dose of 445.68 mg/kg while creatinine level increased in rats treated with both doses. In female rats, a significant decrease (p<0.05) of the level of AST and creatinine was noted in rats treated with the dose of 222.84 mg/kg of D. multiflora. Histological exams of kidney and liver of treated rats showed architectural modifications at the dose of 445.68 mg/kg. This finding suggests that D. multiflora leaf extract is efficient against diarrhea caused by S. flexneri but the treatment with doses lower than 222.84 mg/kg is recommended while further study is required to define the exact efficient nontoxic dose.

Estrogenic properties of spices of the traditional Cameroonian dish “Nkui” in ovariectomized Wistar rats

2016 ◽  
Vol 13 (2) ◽  
Author(s):  
Edwige Nana Tchoupang ◽  
Sylvin Benjamin Ateba ◽  
Stéphane Zingue ◽  
Martin Zehl ◽  
Liselotte Krenn ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Health Benefits ◽  
Ethanol Extract ◽  
Mammary Glands ◽  
Traditional Food ◽  
Uterotrophic Assay ◽  
Female Wistar Rats ◽  
Wet Weight ◽  
Estrogenic Properties

Abstract: Besides the basic role to flavor and color foods, several health benefits have been attributed to spices. The traditional Cameroonian food “Nkui” is prepared using several spices (: OVX female Wistar rats were randomly separated in several groups of five animals each and submitted to a 3-day uterotrophic assay (: These extracts increased the uterine wet weight, the uterine and vaginal epithelial heights, and the lumen and diameter of alveoli in the mammary glands. They also altered the estradiol-induced increase of uterine wet weight. The dichloromethane and methanol fractions of the ethanol extract exhibited estrogenic properties as well by increasing uterine and vaginal endpoints.: These results suggest that the spices of “Nkui” contain estrogenic phytoconstituents and this traditional food may be considered as functional.

scholarly journals Reproductive Function Evaluation in Female Wistar Rats Treated with Aqueous leaf Extract of Cissusaralioides

2019 ◽  
Vol 16 (04) ◽  
pp. 857-864
Author(s):  
Bartholomew Chukwuebuka Nwogueze ◽  
Anthony Emeka Ojieh ◽  
Richard Nduka Ossai ◽  
ChidinmaNwanneamaka Eke
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Reproductive Function ◽  
Function Evaluation ◽  
Eastern Region ◽  
Body Weight Reduction ◽  
Therapeutic Benefits ◽  
The Family ◽  
Female Wistar Rats ◽  
Aqueous Leaf Extract

ABSTRACT: Background: Medicinal plants are increasingly getting recognition. Cissus aralioides is a lofty climber belonging to the family of Vitaceae and widely distributed in South-Eastern region of Nigeria. Varied therapeutic benefits as well as well health harming effects have been reported about the plant. This study evaluated the effects of aqueous extract of Cissus aralioides leaf on reproductive functions in female Wistar rats.Methods: 72 adult female Wistar rats weighing between 110-180/g were used for the study. The animals were randomly grouped into 6 groups of twelve rats each (n=12). Groups 1 and 2 served as control while groups 3 to 6 were the experimental groups and were administered (100/mg, 200/mg, 300/mg and 500/mg)/kg of the extract respectively for 4 weeks. At the end of the experiment, blood samples were collected and centrifuged to obtain the serum for biochemical analysis for Follicle Stimulating Hormone (FSH), Estrogen and Luteinizing Hormone (LH). Data were analyzed using the SPSS package and expressed as mean± SEM and ANOVA.Results: There was significant body weight reduction across groups administered with the extract. Also, there were significant decrease in estrogen level in groups that received 300/mg/kg and 500/mg/kg when compared with the control groups, a similar decrease was not observed with LH and FSH across the groups.Conclusion: Observations from this study shows that aqueous leaf extract of Cissus aralioides decreases circulating levels of estrogen in female Wistar rats.

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