CIRCADIAN VARIATION OP PLASMINOGEN ACTIVATOR INHIBITOR (PAI) AND THE INCIDENCE OP SEVERE ISCHEMIC ATTACKS IN PATIENTS WITH CORONARY ARTERY DISEASE (CAD)
Recent studies have shown a high incidence for the onset of acute myocardial infarction (AMI) in the morning and a possible role of high plasma levels of plasminogen activator inhibitor for the risk of reinfarction. We therefore investigated the diurnal course of PAI and the incidence of severe ischemic attacks in patients with CAD. Blood was drawn from 64 patients at the coronary care unit with angiographically proven CAD (group A = unstable angina, n=37; age = 60.7 ± 10.1 years; group B = stable angina, n=27, age = 58.2 ± 10.9 years) at 4 different time points (6 a.m., noon, 6 p.m., midnight) for at least 3 consecutive days. PAI (functional titration assay; IU t-PA inhibited/ml) and time of onset of severe ischemic attacks including AMI were determined. 50 healthy volunteers (age = 39 ± 17 years) served as controls. We found a significant diurnal variation (paired t-test, p<0.05) of PAI levels in normals (6 am: 11.5±5.6; noon: 6.9±1.7; 6 pm: 5.7±2.5; x ± SD) as well as in patients (6 am: 15.6 ± 6.4; noon: 13-3 ± 1.2; 6 pm: 12.6 ± 5.3; midnight: 13.2 ± 2.8; x ± SD) with an acrophase at 6 am. Compared to the controls PAI values were significantly higher in patients (p<0.01) but showed no significant differences within the two patients’ groups. We could not demonstrate a significant difference in PAI values of patients with or without previous myocardial infarction. Furthermore, development of AMI had no demonstrable influence on preexisting PAI values in both patients’ groups. During the observation period 90 severe ischemic attacks were observed. Attacks occured significantly more often (70$) between midnight and noon with a peak incidence (44$) around 10 am. From these data it can be concluded that PAI levels are elevated in patients with CAD. Peak levels of PAI seem to precede the ischemic event by only a few hours and might thereby contribute to the onset of ischemic events on a basis of increased thrombotic tendency.