DIFFERENT LOW MOLECULAR WEIGHT HEPARINS (LMWHs) ARE NOT IDENTICAL IN THE IN VITRO SCREENING REGARDLESS OF POTENCY ADJUSTMENT
There is considerable controversy in the standardization and potency evaluation of low molecular weight heparins (LMWHs).Since these agents are produced by fractionation or depolymerization procedures, the resulting material shows marked compositional variations regardless of the similarity in molecular weight. In order to address the question “are different LMWHs the same?”, we utilized a uniform multiparametric in vitro test battery. Seven commercial LMWHs Choay Fraxiparine (CY 216), Choay CY 222, Novo LHN, Kabi Fragmin, Opocrin 2123 (OP), Hepar RD 11885 (RD), Pharmuka Enoxaparine (PK) and an unfractionated porcine mucosal heparin (PMH) were studied at equigravimetric amounts in established assays. PMH and LMWHs were ranked acdording to the dose-dependent effects and the results are summarized in the following:As evident from the above data, each of the agents studied showed a distinct profile in the in vitro test system studied. This data suggests that LMWHs are individual drugs with marked differences in in vitro actions, which may not be the true determinants of the relative safety/efficacy in clinical settings.