Haemostatic Parameters and Lifestyle Factors in Elderly Men in Italy and The Netherlands

1996 ◽  
Vol 76 (03) ◽  
pp. 411-416 ◽  
Author(s):  
Fransje C H Bijnen ◽  
Edith J M Feskens ◽  
Simona Giampaoli ◽  
Alessandro Menotti ◽  
Flaminio Fidanza ◽  
...  
Keyword(s):  
Alcohol Use ◽  
The Netherlands ◽  
Antithrombin Iii ◽  
Lifestyle Factors ◽  
Activated Partial Thromboplastin Time ◽  
Factor Vii ◽  
Fat Intake ◽  
Factor X ◽  
Elderly Men ◽  
History Of

SummaryThe association between plasma fibrinogen, factor VII, factor X, activated partial thromboplastin time, antithrombin III and the lifestyle factors cigarette smoking, alcohol use, fat intake and physical activity was assessed in 802 men aged 70-90 years in Zutphen (The Netherlands), Montegiorgio and Crevalcore (Italy).Smoking was positively associated with fibrinogen, also after adjustment for other lifestyle factors, age, use of anticoagulants and aspirin like drugs, body mass index, and history of myocardial infarction. Alcohol use was associated with increased levels of factor X and decreased levels of antithrombin III. Fat intake was positively associated with antithrombin III. Between cohorts, considerable differences were observed in levels of haemostatic parameters and the lifestyle factors. Compared to the mediterranean cohorts the Zutphen cohort showed the highest levels of fibrinogen and factor VII. Differences in lifestyle factors could, however, not explain differences between cohorts in levels of any of the haemostatic parameters, despite the observed associations between lifestyle factors and haemostatic parameters.

The rise of factor X level in blood plasma of patients at severe burn injuries

2021 ◽  
Author(s):  
George P Kozynets ◽  
Volodymyr P Tsyhankov ◽  
Daria S Korolova ◽  
Olga V Gornytska ◽  
Olexiy M Savchuk ◽  
...  
Keyword(s):  
Protein C ◽  
Antithrombin Iii ◽  
Burn Injury ◽  
Activated Partial Thromboplastin Time ◽  
Burn Injuries ◽  
Factor X ◽  
Severe Burn ◽  
Clotting Factors ◽  
Thrombotic Complications ◽  
Severe Burn Injuries

Abstract This work is dedicated to the detection of imbalance between the pro- and anti-coagulant branches of hemostasis at severe burn injuries by evaluating the content or activity of individual clotting factors. To select the targets for accurate diagnostics we measured the concentrations of soluble fibrin monomeric complexes and fibrinogen, levels of total prothrombin, factor X, protein C and antithrombin III, and recorded the time of clotting in activated partial thromboplastin time and prothrombin time tests. Factor X level was increased in 26 % of patients on the first day after the burn and it rose further in 62 % patients on the 14 th day of recovery. Increasing factor X level is assumed to be a risk factor of thrombotic complications. We propose to use it as a marker of predisposition to thrombosis at severe burn injury.

ASSOCIATION OF PHYSICAL ACTIVITY AND OTHER LIFESTYLE FACTORS WITH FIBRINOGEN AND FACTOR VII IN ELDERLY MEN 863

1996 ◽  
Vol 28 (Supplement) ◽  
pp. 145
Author(s):  
F CH Bijnen ◽  
E JM Feskens ◽  
S Giampaoli ◽  
A Menotti ◽  
G Hornstra ◽  
...  
Keyword(s):  
Physical Activity ◽  
Lifestyle Factors ◽  
Factor Vii ◽  
Elderly Men

Effect of a Moderate Fish Intake on Haemostatic Parameters in Healthy Males

1989 ◽  
Vol 61 (03) ◽  
pp. 468-473 ◽  
Author(s):  
A D Muller ◽  
A C v Houwelingen ◽  
M C E van Dam-Mieras ◽  
B M Bas ◽  
G Hornstra
Keyword(s):  
Intervention Study ◽  
Dietary Intervention ◽  
Antithrombin Iii ◽  
Experimental Period ◽  
Procoagulant Activity ◽  
Factor Vii ◽  
Factor X ◽  
Activated Factor Vii ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryThis article describes the results of a dietary intervention study performed in three different centers. In the study the effect of a diet enriched with fish on the coagulation tendency of blood was investigated. Two groups of 40 volunteers were given a dietary supplement consisting of 135 g of canned mackerel or meat paste (control) for a 6 weeks period.Compliance, monitored by measuring the urinary excretion of lithium, added to the supplements, was about 80%. Before, during and at the end of the experimental period a number of hemostatic parameters, reflecting the coagulation tendency of blood and the procoagulant activity of monocytes, were measured.The fish supplement did not cause a significant effect on the prothrombin time and on the levels of factor VII, activated factor VII, antithrombin III, von Willebrand factor, fibrinogen, plasminogen and a2-antiplasmin. A slight but transient prolongation in the activated partial thromboplastin time was observed as well as a significant increase in the factor X level, which became more pronounced with prolongation of the experimental period; no activated factor X was found.A tendency towards a stimulation of monocyte procoagulant activity was noticed.

scholarly journals Activation of human factor VII by activated factors IX and X

Blood ◽  
1982 ◽  
Vol 60 (5) ◽  
pp. 1143-1150 ◽  
Author(s):  
DR Masys ◽  
SP Bajaj ◽  
SI Rapaport
Keyword(s):  
Human Factors ◽  
Factor Viii ◽  
Active Site ◽  
Human Factor ◽  
Antithrombin Iii ◽  
Factor Ix ◽  
Factor Vii ◽  
Activate Factor ◽  
Factor X ◽  
Activated Factor Vii

Factor VII clotting activity increases about five-fold when blood is clotted in glass. Prior studies suggested that this results from activation induced by activated factor IX (IXa). However, in purified systems containing phospholipid and calcium, activated factor X (Xa) is known to activate factor VII rapidly. Therefore, we studied activation of factor VII by IXa and X, in systems using purified human factors. Concentrations of IXa and Xa were calculated from total activated protein concentrations rather than from active site concentrations. In the presence of phospolipid and calcium, both IXa and Xa activated factor VII 25-fold; however, Xa was roughly 800 times more efficient than IXa. Without added phospholipid, activation of factor VII by both Xa and IXa was markedly slowed, and Xa was roughly 20 times more efficient than IXa. When both phospholipid and calcium were omitted, activation of factor VII by either enzyme was negligible. Adding normal prothrombin, but not decarboxylated prothrombin, substantially slowed activation of factor VII by both Xa and IXa. Adding thrombin-activated factor VIII and antithrombin-III did not change rates of factor VII activation by either enzyme. These results from purified systems do not provide an explanation for the prior data from plasma systems.

Doxycycline-induced acquired haemophilia A

2021 ◽  
Vol 14 (10) ◽  
pp. e244748
Author(s):  
Ejaz Shah ◽  
Calvin Abro ◽  
Fawwad Zaidi ◽  
Ruchika Goel
Keyword(s):  
Acute Respiratory Distress Syndrome ◽  
Respiratory Failure ◽  
Factor Viii ◽  
Distress Syndrome ◽  
Therapeutic Agents ◽  
Activated Partial Thromboplastin Time ◽  
Factor Vii ◽  
Haemophilia A ◽  
Acquired Haemophilia ◽  
History Of

An 80-year-old man with no personal or family history of bleeding, presented to hospital with extensive haematomas and skin bruising after using doxycycline. His basic lab workup was concerning for a coagulopathy with an elevated activated partial thromboplastin time and significant anaemia. Mixing studies and other factor levels were tested that led to the diagnosis of acquired haemophilia A with low factor VIII levels and high factor VIII antibodies. He was started on steroids, but his haemoglobin level continued to drop. Later, during his treatment, he was given multiple therapeutic agents, including cyclophosphamide, rituximab and recombinant factor VII (NovoSeven-R). Gradually factor VIII levels increased and haemoglobin stabilised. The hospital course was complicated by COVID-19 pneumonia leading to acute respiratory distress syndrome; the patient eventually expired due to respiratory failure.

THE CATALYTIC TRIAD RESIDUES (HIS221, ASP269, SER365) AND THE BINDING POCKET RESIDUE (ASP359) IN FACTOR IXBm ELSINORE (IXBmLE) ARE NOT ALTERED

1987 ◽  
Author(s):  
S G Spitzer ◽  
P Usharani ◽  
A D Roser ◽  
C K Kasper ◽  
S G Bajaj
Keyword(s):  
Catalytic Domain ◽  
Antithrombin Iii ◽  
Genomic Library ◽  
Indirect Evidence ◽  
Binding Pocket ◽  
Catalytic Triad ◽  
Factor Vii ◽  
Factor X ◽  
Liver Cdna Library ◽  
Synthetic Oligonucleotides

Previous studies suggested that the defect in IXBmLE (a nonfunctional variant of human IX) is either in the catalytic triad or at the site(s) of interaction with the macromolecular substrates (antithrombin III, factor VII or factor X). To distinguish between these possibilities, we isolated a complete IX cDNA clone from a human liver cDNA library. We also constructed a genomic library (in phage EMBL3) using DNA of the BmLE patient. The library was screened with normal IX cDNA and with synthetic oligonucleotides. The positive clones containing the exons coding for IX were plaque purified. Two clones which contained the coding sequence of the catalytic domain, i.e., His221 (exon VII), and Asp269, Asp359, and Ser365 (exon VIII) were selected for further studies. The phage containing exon VIII was first digested with Sail and EcoRI and a 2-Kb fragment, which hybridized with the segment of cDNA containing exon VIII, was gel purified. The 2-Kb fragment was further digested and the subfragments were cloned into M13; the length and direction of the fragments used in sequencing are shown below:The phage containing exon VII was digested with PstI and SalI, and a 1-Kb fragment that hybridized with the 19-mer His221 probe was subcloned into M13 phage for sequencing. The sequence starting with residue Vall96 through residue Arg403 was found to be normal. Thus, none of the residues in the catalytic domain of IXBmLE are different from that of normal IX. These data provide strong indirect evidence that the noncatalytic aminoterminal portion of IX plays a significant role in the structural recognition of the macromolecular substrates. The sequence of this region of IXBmLE should provide information about the putative residue(s) essential for this recognition.

THE MOLECULAR WEIGHT DEPENDENT EFFECTS OF HEPARIN ON THROMBOPLASTIN ACTIVATED PROTEASES

1987 ◽  
Author(s):  
R M Emanuele ◽  
J C Lormeau ◽  
J Choay ◽  
J Fareed
Keyword(s):  
Molecular Weight ◽  
Prothrombin Time ◽  
Antithrombin Iii ◽  
Gel Filtration ◽  
Factor Vii ◽  
Fibrinopeptide A ◽  
Factor X ◽  
Dependent Effect ◽  
Generation Test ◽  
Different Molecular Weight

Five different molecular weight (M. W.) fractions of heparin (M. W.'s 23,000 ; 17,450 ; 13,300 ; 9,000 and 5,100) were prepared by gel-filtration. Screening of these fractions in global and amidolytic assaysat a concentration of 2.5 ug/ml revealed a dependence on M. W. for the potency response. In the APTT, TT, Heptest, amidolytic anti Xa and Ila assays, an increase in potency was observed with increasing M. W.. This relationship remained consistent to 13,300 M. W. after which no further increases in potency were observed. However, when the same fractions were screenedfor their effect on prothrombin time (PT), a different M. W. dependent pattern was observed. In this assay, a continued increase in potency was observed for the 17,450 and 23,000 M. W. fractions. At a concentration of 20 ug/ml, the 23,000 M. M. fraction produced the largest prolongation of the PT while the 5,100 M. W. fraction showed the least effect. The correlation between M. W. and potency in the PT assay was 0.98. No M. W. dependent potency response was observed whenidentical PT.'s were performed in plasma deficient in antithrombin-III. Further testing of the M. W. fractions using a Ca++/thromboplastin activated fibrinopeptide-A generation test resulted in a potency response similar to that observed in the PT. To more precisely investigate this M. W. dependent effect upon thromboplastin activation an amidolytic assay using factor VII-thromboplastin to convert factor X to Xa wasdeveloped. The inhibition of Xa in the presence of the M. W. fractions and antithrombin III was used as the assay endpoint. As with previous assays involvingthromboplastin activation, potency increased with increasing M. W. from 5,100 through 23,000. These observations coupled with the failure of amidolytic anti Xa assays to show increased potency beyond 13,000 M. W., suggest that heparin of high M. W. may beacting at the level of thromboplastin or factor VII / VIIa.

Hemostatic profile of bovine ovarian follicular fluid

1995 ◽  
Vol 73 (5) ◽  
pp. 624-629 ◽  
Author(s):  
Manabu Yamada ◽  
Patricia A. Gentry
Keyword(s):  
Prothrombin Time ◽  
Follicular Fluid ◽  
Inhibitory Activity ◽  
Antithrombin Iii ◽  
Factor Vii ◽  
Coagulation System ◽  
Factor X ◽  
Low Levels ◽  
Coagulation Pathway ◽  
Ovarian Follicular Fluid

The hemostatic profile of bovine ovarian follicular fluid was evaluated and the levels of procoagulant, fibrinolytic, and inhibitory activity compared with plasma. The results of the prothrombin time assay and the presence of fibrinogen along with factor VII and factor X activity indicate that bovine follicular fluid possesses components of the "extrinsic" or "tissue factor" coagulation system. The absence of factor VIII:C activity, along with the extremely low levels of factors IX and XI, indicates that there is not a functional "intrinsic" coagulation pathway. The fluid derived from large follicles exhibited increased levels of factors VII and X activity and a shorter prothrombin time compared with fluid obtained from the less mature small follicles. Similar alterations in the levels of the inhibitory proteins antithrombin III and α2-macroglobulin were observed. Overall the amount of antithrombin III was similar to that in plasma, the levels of fibrinogen and factor X were approximately 2-fold lower, and the levels of factor VII and factor X were approximately 10-fold lower than in plasma. The fibrinolytic activity in follicular fluid was greater than the procoagulant or inhibitory activity. Plasminogen activator activity was 5-foid higher, while both plasminogen and antiplasmin values were similar to plasma levels.Key words: hemostasis, follicular fluid, bovine.

Coagulability of Blood and Fibrinolysis Related to Age and Lipids

1975 ◽  
Author(s):  
T. Matsuda
Keyword(s):  
Factor Viii ◽  
Antithrombin Iii ◽  
Normal Subjects ◽  
Factor Vii ◽  
Factor V ◽  
Factor X ◽  
Progressive Decline ◽  
Cholesterol Levels ◽  
Α2 Macroglobulin ◽  
Older Subjects

Levels of factor VIII, factor VII-X, factor X, factor V, fibrinogen, antithrombin III, euglobulin lysis times, plasminogen, α2-macroglobulin, α1-antitrypsin and FDP were determined in 487 healthy normal subjects from age 20 to 94. In subjects over age 60, serum lipids were determined simultaneously.In the subjects over age 60, levels of factor VIII, fibrinogen, antithrombin III, α2-maeroglobulin, α1-antitrypsin and FDP were higher, factor V activity was lower, and euglobulin lysis times were shorter than in subjects under age 50. In the older subjects, levels of factor VII-X, factor X fibrinogen, antithrombin III, plasminogen and FDP showed slight but progressive decline with age. In these subjects over age 60, serum cholesterol levels were significantly correlated with levels of factor VII-X, factor X, antithrombin III, plasminogen and FDP; antithrombin III concentration was significantly correlated with factor X activity but not with factor VII-X activity; euglobulin lysis times were correlated with obesity.

The Activated Partial Thromboplastin Time After Heparin Removal (aPTT/HR): The Basis Of A New Single Test Stratagem For Monitoring All Phases Of Anticoagulation With Heparin And/Or Coumarins

1981 ◽  
Author(s):  
H E Branson ◽  
C Engelberg ◽  
A Fagin ◽  
S Puri ◽  
J Broadbrooks
Keyword(s):  
Partial Thromboplastin Time ◽  
Antithrombin Iii ◽  
Activated Partial Thromboplastin Time ◽  
Factor X ◽  
Single Test ◽  
Clin Pathol ◽  
Coagulation Therapy ◽  
Monitoring Therapy ◽  
Alternative Test ◽  
Clinically Significant

Safe and accurate monitoring of the transition from the antithrombotic protection afforded by the heparin-antithrombin III complex to that of coumarin modified zymogens has traditionally been provided by special dilutional one-stage prothrombin times. The demonstrated ability of ECTEOLA anion exchange columns to remove heparin from plasma containing acarboxy forms of the vitamin k dependent factors led to the development of an alternative test, the protime after heparin removal (PT/HR) (Am J Clin Pathol 71 (6): 665, 1979). Application of this same technology to the activated partial thromboplastin time (aPTT/HR) results in: 1) a superior alternative to dilutional and ion exchange protimes; and 2) a new single test/pathway scheme for following the heparin, heparin and coumarin, and coumarin phases of anti-coagulation therapy. The fitness of the aPTT for monitoring therapy with heparin alone and coumarins alone has been previously established. Preliminary studies indicate that the aPTT/HR is very similar in its ability to determine the adequacy of anticoagulation to a combination of the PT and aPTT. Comparison of the aPTT/HR with the PT/HR for combined heparin and coumarin monitoring indicated that it: 1) more closely approximates the functional activity of factor X; 2) is equally sensitive to heparin and coumarins; 3) is responsive to more common clinically significant depressions of factors; and 4) is the test of choice for pre-surgical screening.

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