Exploring molecular basis of therapy response in patient derived primary liver cancer cell lines

2019 ◽  
Author(s):  
D Castven ◽  
D Becker ◽  
C Czauderna ◽  
D Wilhelm ◽  
JB Andersen ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Molecular Basis ◽  
Primary Liver Cancer ◽  
Therapy Response ◽  
Cancer Cell Lines ◽  
Liver Cancer Cell

scholarly journals Underutilized Mexican Plants: Screening of Antioxidant and Antiproliferative Properties of Mexican Cactus Fruit Juices

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 368
Author(s):  
Elda M. Melchor Martínez ◽  
Luisaldo Sandate-Flores ◽  
José Rodríguez-Rodríguez ◽  
Magdalena Rostro-Alanis ◽  
Lizeth Parra-Arroyo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Liver Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
In Vitro Assays ◽  
Total Phenolic ◽  
Liver Cancer Cell ◽  
Antiproliferative Activities

Cacti fruits are known to possess antioxidant and antiproliferative activities among other health benefits. The following paper evaluated the antioxidant capacity and bioactivity of five clarified juices from different cacti fruits (Stenocereus spp., Opuntia spp. and M. geomettizans) on four cancer cell lines as well as one normal cell line. Their antioxidant compositions were measured by three different protocols. Their phenolic compositions were quantified through high performance liquid chromatography and the percentages of cell proliferation of fibroblasts as well as breast, prostate, colorectal, and liver cancer cell lines were evaluated though in vitro assays. The results were further processed by principal component analysis. The clarified juice from M. geomettizans fruit showed the highest concentration of total phenolic compounds and induced cell death in liver and colorectal cancer cells lines as well as fibroblasts. The clarified juice extracted from yellow Opuntia ficus-indica fruit displayed antioxidant activity as well as a selective cytotoxic effect on a liver cancer cell line with no toxic effect on fibroblasts. In conclusion, the work supplies evidence on the antioxidant and antiproliferative activities that cacti juices possess, presenting potential as cancer cell proliferation preventing agents.

scholarly journals Specific Inhibition of the Redox Activity of Ape1/Ref-1 by E3330 Blocks Tnf-Α-Induced Activation of Il-8 Production in Liver Cancer Cell Lines

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e70909 ◽  
Author(s):  
Laura Cesaratto ◽  
Erika Codarin ◽  
Carlo Vascotto ◽  
Antonio Leonardi ◽  
Mark R. Kelley ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Redox Activity ◽  
Specific Inhibition ◽  
Liver Cancer Cell ◽  
Tnf Α

scholarly journals Anti-Proliferative Effects of an Extra-Virgin Olive Oil Extract Enriched in Ligstroside Aglycone and Oleocanthal on Human Liver Cancer Cell Lines

Cancers ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1640 ◽  
Author(s):  
De Stefanis ◽  
Scimè ◽  
Accomazzo ◽  
Catti ◽  
Occhipinti ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cancer ◽  
Olive Oil ◽  
Cell Lines ◽  
Cancer Cell ◽  
Virgin Olive Oil ◽  
Cancer Cell Lines ◽  
Liver Cancer Cell ◽  
Antioxidant Power ◽  
Polyphenolic Extract

Oleocanthal and ligstroside aglycone are olive oil-derived polyphenols. The former interferes with tumor growth with minor or no cytotoxicity on non-tumorigenic primary cell lines. The information about the bioactivity of ligstroside aglycone are scanty, with the exception of a known antioxidant power. Hepatocellular carcinoma is a malignant tumor with high mortality rates. Systemic chemotherapy is only marginally effective and is frequently complicated by toxicity. Previous observations have shown that hepatocellular carcinoma cell lines become more sensitive to taxol when it is combined with Tumor Necrosis Factor α (TNFα). The present work aimed to assess the effects of a polyphenolic extract containing both oleocanthal and ligstroside aglycone on proliferation and/or death in three liver cancer cell lines (HepG2, Huh7 and Hep3B). The possibility to enhance such effect by the addition of TNFα was also investigated. Both cell proliferation and death were enhanced by the exposure to the polyphenolic extract. Such effect was associated with induction of autophagy and could be potentiated by TNFα. The presence of ligstroside aglycone in the extract lowered the oleocanthal concentration required for cytotoxicity. These results show for the first time that the effects of a polyphenol extract can be potentiated by TNFα and that modulation of autophagy likely account for these effects.

Abstract 2489: Sensitivity of liver cancer cell lines to B-catenin knock-down correlates with pathway activation

2018 ◽  
Author(s):  
Zhihu (Jeff) Ding ◽  
Chaomei Shi ◽  
Lan Jiang ◽  
Tatiana Tolstykh ◽  
Hui Cao ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Liver Cancer Cell ◽  
Knock Down ◽  
Pathway Activation

scholarly journals Quercetin Suppresses Proliferation of Liver Cancer Cell Lines In Vitro

2020 ◽  
Vol 40 (8) ◽  
pp. 4695-4700 ◽  
Author(s):  
TORU HISAKA ◽  
HISAMUNE SAKAI ◽  
TOSHIHIRO SATO ◽  
YUICHI GOTO ◽  
YORIKO NOMURA ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Liver Cancer Cell

scholarly journals IN VITRO GROWTH INHIBITORY EFFECT OF ZnO NANOPARTICLES ON HUMAN LIVER CANCER CELL LINES (Huh7)

2019 ◽  
pp. 191-193
Author(s):  
ANANTHALAKSHMI R ◽  
XAVIER RAJARATHINAM SR ◽  
Mohamed Sadiq A ◽  
MOHAMED SADIQ A
Keyword(s):  
Liver Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Zno Nanoparticles ◽  
Huh7 Cell ◽  
Cancer Cell Lines ◽  
Inhibitory Effect ◽  
Liver Cancer Cell ◽  
Zno Nps

Objective: The objective of the study was to access the anticancer activity of the biosynthesized ZnO nanoparticles against Huh7 liver cancer cell lines. Methods: The study was carried in vitro using Huh7 cell lines. The ZnO nanoparticles (ZnO NPs) were synthesized using Luffa acutangula peel extract and subjected to characterization by X-ray powder diffraction and transmission electron microscopy. The Huh7 cell lines were treated with ZnO NPs and done 3-(4, 5-dimethylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide assay. For live and dead assay, the cell lines treated with ZnO NPs were subjected to acridine orange/ethidium (AO/ET) bromide assay. Results: The ZnO NPs synthesized show spherical structure with 10–20 nm size. The 50% of Huh7 proliferation were inhibited at the concentration (IC50) of 40 μg/ml. The AO/ET assay shows compact nucleus and fine cytoplasmic morphology in control cells and apoptotic stage in treated cells Conclusion: This study suggests that ZnO NPs can be prepared in environment-friendly method using aqueous extract of L. acutangula and can be used in cancer treatment effectively.

Sign in / Sign up

Export Citation Format

Share Document