Investigation of ellagic acid sourcing as a dietary supplement by UFLC-PDA-ESI-MS/MS

An LC-DAD-ESI-MSn technique was developed to investigate crude extracts of the stem bark of S. guineense for phenolic compounds. Ellagic acids were detected on the basis of their UV absorption spectra and ESI-MS fragmentation patterns. Six ellagic acid conjugates in the complex extract of S. guineense were identified. With the aim to confirm the structures, further purification of the extract afforded the known 3-O-ellagic acid-4′-O-α-rhampyranonosides (3), two new ellagic acid rhamnopyranosides, 3-O-methylellagic acid-4′-O-α-2″-O-acetylrhamnopyranoside (4) and 3-O-methylellagic acid-4′-O-α-3″-O-acetylrhamnopyranoside (6). The structures were identified on the basis of 1D- and 2D- NMR, IR, and UV spectroscopic and mass spectrometric data. The HMBC and NOESY data of these compounds were most useful for determination of their structures. Radical scavenging activities were evaluated in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Compounds 3, 4 and 6, with IC50 values of 6.1 ± 0.5, 5.8 ± 0.4, and 6.2 ± 0.4 μM, respectively were as active as ellagic acid (1) (IC50 = 4.9 ± 0.3 μM) and more active than catechin (IC50=10.8 ± 0.3 μM).

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HPLC/MS-MS Identification of Oak Quercus aegilops Root Tannins

Journal of Chemistry ◽

10.1155/2021/8882050 ◽

2021 ◽

Vol 2021 ◽

pp. 1-10

Author(s):

Ayed S. Amr ◽

Mousa N. Ahmad ◽

Jalal A. Zahra ◽

Mai A. Abdullah

Keyword(s):

Ellagic Acid ◽

Acid Ester ◽

Polyphenolic Compounds ◽

Root Bark ◽

Biological Functions ◽

Plant Kingdom ◽

Esi Ms ◽

Oak Tree ◽

Common Oak ◽

Ms Search

Tannins are natural polyphenolic compounds widely distributed in the plant kingdom in the leaves, bark, fruits, and other parts. They have various biological functions in humans and animals and are used mainly in the pharmaceutical and cosmetic industries. The aim of this work was to isolate, extract, purify, and identify the tannins from the root bark of a common oak tree (Quercus aegilops L.) in Jordan and around the Mediterranean. The results showed that at least one form of ellagitannin (ellagic acid ester), quercitrin, afzelechin, valoneic acid, trigalloyl glucose, and catechin was identified in addition to two unidentified compounds. Results of this work can help in developing an ESI MS/MS search library for the constituents of the tannins of oak (Quercus aegilops L.) root bark.

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Determination of Polar Compounds in Guava Leaves Infusions and Ultrasound Aqueous Extract by HPLC-ESI-MS

Journal of Chemistry ◽

10.1155/2015/250919 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 14

Author(s):

Elixabet Díaz-de-Cerio ◽

Vito Verardo ◽

Ana María Gómez-Caravaca ◽

Alberto Fernández-Gutiérrez ◽

Antonio Segura-Carretero

Keyword(s):

Antioxidant Activity ◽

Aqueous Extract ◽

Ellagic Acid ◽

Polar Compounds ◽

Optimal Time ◽

Esi Ms ◽

Conventional Ultrasound ◽

Ellagic Acid Derivatives ◽

Guava Leaves

Literature lacks publications about polar compounds content in infusion or guava leaves tea. Because of that, a comparison between different times of infusion and a conventional ultrasound aqueous extract was carried out. Several polar compounds have been identified by HPLC-ESI-MS and their antioxidant activity was evaluated by FRAP and ABTS assays. Four different classes of phenolic compounds (gallic and ellagic acid derivatives, flavonols, flavanones, and flavan-3-ols) and some benzophenones were determined. The quantification results reported that the order, in terms of concentration of the classes of polar compounds in all samples, was flavonols > flavan-3-ols > gallic and ellagic acid derivatives > benzophenones > flavanones. As expected, the aqueous extract obtained by sonication showed the highest content in the compounds studied. Significative differences were noticed about the different times of infusion and five minutes was the optimal time to obtain the highest content in polar compounds using this culinary method. All the identified compounds, except HHDP isomers and naringenin, were positively correlated with antioxidant activity.

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Quantitative analysis of phenolic compounds in crude extracts of Myrcia splendens leaves by HPLC-ESI-MS/MS

Rodriguésia ◽

10.1590/2175-7860202071045 ◽

2020 ◽

Vol 71 ◽

Cited By ~ 1

Author(s):

Camila Jeriane Paganelli ◽

Diogo Alexandre Siebert ◽

Luciano Vitali ◽

Gustavo Amadeu Micke ◽

Michele Debiasi Alberton

Keyword(s):

Salicylic Acid ◽

Quantitative Analysis ◽

Phenolic Compounds ◽

Ferulic Acid ◽

Ethyl Acetate ◽

Ellagic Acid ◽

Syringic Acid ◽

Coumaric Acid ◽

Southern Brazil ◽

Esi Ms

Abstract Myrcia splendens is popularly known as “guamirim-de-folha-miúda”, and its occurrence ranges from Mexico to southern Brazil. The aim of this work was to identify and quantify phenolic compounds in the crude hydroalcoholic (EBH), ethyl acetate (EBAE) and dichloromethane (EBDM) extracts using the HPLC-ESI- MS/MS. In total, 15 compounds, including protocatecuic acid, syringic acid, p-coumaric acid, salicylic acid, isoquercetin, ellagic acid, ferulic acid, umbelliferone, coniferaldehyde, sinapaldehyde, carnosol, gallic acid, syringaldehyde, umbelliferone, coniferaldehyde, myricetin and kaempferol were identified. Ellagic acid was the major compound in all extracts.

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