scholarly journals Quantitative analysis of phenolic compounds in crude extracts of Myrcia splendens leaves by HPLC-ESI-MS/MS

Rodriguésia ◽  
2020 ◽  
Vol 71 ◽  
Author(s):  
Camila Jeriane Paganelli ◽  
Diogo Alexandre Siebert ◽  
Luciano Vitali ◽  
Gustavo Amadeu Micke ◽  
Michele Debiasi Alberton
Keyword(s):  
Salicylic Acid ◽  
Quantitative Analysis ◽  
Phenolic Compounds ◽  
Ferulic Acid ◽  
Ethyl Acetate ◽  
Ellagic Acid ◽  
Syringic Acid ◽  
Coumaric Acid ◽  
Southern Brazil ◽  
Esi Ms

Abstract Myrcia splendens is popularly known as “guamirim-de-folha-miúda”, and its occurrence ranges from Mexico to southern Brazil. The aim of this work was to identify and quantify phenolic compounds in the crude hydroalcoholic (EBH), ethyl acetate (EBAE) and dichloromethane (EBDM) extracts using the HPLC-ESI- MS/MS. In total, 15 compounds, including protocatecuic acid, syringic acid, p-coumaric acid, salicylic acid, isoquercetin, ellagic acid, ferulic acid, umbelliferone, coniferaldehyde, sinapaldehyde, carnosol, gallic acid, syringaldehyde, umbelliferone, coniferaldehyde, myricetin and kaempferol were identified. Ellagic acid was the major compound in all extracts.

Identification of Mentha piperita L. and Ricinus communis L. polyphe-nols by HPLC-DAD-ESI-MS and evaluation of their insecticidal properties against Aphis spiraecola P

2017 ◽  
Vol 7 (1) ◽  
pp. 28-34
Author(s):  
Malika Malika Boualem ◽  
Meriem Mokhtar ◽  
Farida Saiah ◽  
Fouzia Benourad ◽  
Radia Bouhadiba ◽  
...  
Keyword(s):  
Ricinus Communis ◽  
Syringic Acid ◽  
Mentha Piperita ◽  
Ionization Mass ◽  
Coumaric Acid ◽  
Esi Ms ◽  
Aphis Spiraecola ◽  
Ricinus Communis L ◽  
Array Detection ◽  
Dose Dependent

Aphids infest most crops, and are considered as one of the most destructive groups of insects in temperate regions. The purpose of this study was to identify the polyphenols of castor (Ricinus communis L.) and mint (Mentha piperita L.) with liquid chromatography coupled to diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS), and evaluate their insecticidal properties against Aphis spiraecola P. A total of 10 compounds were identified in castor mainly phenolic acids (gallic acid, protocatechuic acid, syringic acid, chlorogenic acid, gentisic acid and p-coumaric acid). On the other hands, flavonoids (catechin, naringin, diosmin, rutin, diosmetin, luteolin, neohesperidin, naringenin and apigenin) were the major polyphe-nols in mint representing 10 compounds out of 14. In the test of insecticidal activity, four concentrations were tested (5, 10, 20 and 40%). Both plants had a good inhibitory activity especially mint. The effect was dose dependent and the highest mortality was obtained with the maximum used concentration (40%). After the first day, a percentage of 44 and 76% of mortality was recorded in the presence of just 5% of castor and mint which increased to reach 100% after 7 days.

scholarly journals The effects of plant density and irrigation on phenolic content in cauliflower 

2017 ◽  
Vol 44 (No. 4) ◽  
pp. 178-185 ◽  
Author(s):  
Alina Kałużewicz ◽  
Jolanta Lisiecka ◽  
Monika Gąsecka ◽  
Włodzimierz Krzesiński ◽  
Tomasz Spiżewski ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ferulic Acid ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
Plant Density ◽  
Phenolic Content ◽  
Sinapic Acid ◽  
Dry Weight ◽  
Coumaric Acid

This study was conducted to study the influence of plant density and irrigation on the content of phenolic compounds, i.e., phenolic acids and flavonols in cv. ‘Sevilla’ cauliflower curds. Levels of phenolic acids and flavonols were in the range of 3.0–6.2 mg and 25.4–87.8 mg/100 g of dry weight, respectively, depending on plant density and irrigation. Of the phenolic acids, caffeic acid was detected in the highest amount, followed by p-coumaric acid, sinapic acid, gallic acid, and ferulic acid. Of the two flavonols detected, the levels of quercetin were higher than those of kaempferol. The content of the detected phenolic acids (with the exception of ferulic acid) and both flavonols increased with increasing plant density. Furthermore, the concentration of phenolic compounds (with the exception of ferulic acid) was significantly higher under irrigation.

scholarly journals Ferulic and coumaric acids, total phenolic compounds and their correlation in selected oat genotypes

2008 ◽  
Vol 25 (No. 6) ◽  
pp. 325-332 ◽  
Author(s):  
M. Kováčová ◽  
E. Malinová
Keyword(s):  
Phenolic Compounds ◽  
Ferulic Acid ◽  
Functional Foods ◽  
Slovak Republic ◽  
Total Content ◽  
Climatic Conditions ◽  
Plant Production ◽  
Total Phenolic ◽  
Coumaric Acid ◽  
Total Phenolic Compounds

Ferulic and coumaric acids were determined by high-performance liquid chromatography in 21 oat genotypes supplied from the gene bank of the Research Institute of Plant Production in Piešťany (Slovak Republic) with the aim of selecting some genotypes suitable for the preparation of functional foods. The content of coumaric acid was higher than that of ferulic acid in 61.9% of samples. The latter acid exceeded proportionally the former one in genotypes with a low content of phenolic acids, namely in the amount of up to 50 mg/100 g of grain, especially in chaffless oats. Ferulic acid content varied in the range from 16.50 mg/100 g (cultivar Jakub) to 149.36 mg/100 g of grain (cultivar Izak). The content of coumaric acid ranged between 8.05 mg/100 g (cultivar Detvan) and 210.27 mg/100 g of grain (cultivar Izak). The fact that the samples were grown in the same year (2003) in one locality (Vígľaš-Pstruša, Slovak Republic) minimises the influence of soil and climatic conditions and proves that remarkable differences between the content of ferulic and coumaric acids and the total content of phenolic compounds were caused by the genetic outfit of oats. A high correlation was found between the contents of the total phenolic compounds, ferulic acid, coumaric acid and their sum. The corresponding correlation coefficients R had the values of 0.9229, 0.9141, and 0.9211, respectively. This correlation enables one to employ a simple and inexpensive method for the determination of the total phenolic compounds using Folin-Ciocalteu reagent for a rapid assessment of differences detectable in the content of the sum of ferulic and coumaric acids in oat samples in view of the selection of genotypes suitable for the preparation of functional foods.

scholarly journals Standardization ofTragopogon graminifoliusDC. Extract Based on Phenolic Compounds and Antioxidant Activity

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Mohammad Hosein Farzaei ◽  
Mahnaz Khanavi ◽  
Ghazaleh Moghaddam ◽  
Farzaneh Dolatshahi ◽  
Roja Rahimi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Ferulic Acid ◽  
Aerial Part ◽  
Antioxidant Properties ◽  
Dosage Forms ◽  
Hplc Method ◽  
Total Phenolic ◽  
Root Extract ◽  
Coumaric Acid

Tragopogon graminifoliusDC. (TG), Compositae family, is traditionally used for the treatment of various diseases like gastrointestinal and hepatic disorders. The aim of the present study is to standardize extracts from TG used for preparation of different dosage forms in traditional Iranian medicine (TIM) based on phenolic compounds. For this purpose, total phenolic content and some phenolic compounds were determined in ethanolic extracts from aerial part and root of TG by HPLC method. Furthermore, antioxidant activity was evaluated using DPPH-HPLC methods. Caffeic acid, gallic acid,ρ-coumaric acid, ferulic acid, and catechin were detected in root and aerial part of TG.ρ-Coumaric acid (6.357 ± 0.014 mg·g−1) was dominant phenolic compound in aerial part followed by ferulic acid (1.24 ± 0.018 mg·g−1). Also,ρ-coumaric acid (2.685 ± 0.031 mg·g−1) was highly abundant in root, followed by catechin (2.067 ± 0.021 mg·g−1). Antioxidant activity of root extract (460.45 ± 0.78 µg Vit.E.E·mL−1) was better than that of aerial part. Generally, phenolic compounds are one of the major constituents of TG and could be used as markers for standardization of dosage forms prepared from this plant. Also, TG demonstrated significant antioxidant activity using DPPH-HPLC method. Phenolic compounds of TG may be responsible for its marked antioxidant properties.

scholarly journals Biodegradation of lignin monomers and bioconversion of ferulic acid to vanillic acid by Paraburkholderia aromaticivorans AR20-38 isolated from Alpine forest soil

2021 ◽  
Vol 105 (7) ◽  
pp. 2967-2977
Author(s):  
Rosa Margesin ◽  
Georg Volgger ◽  
Andreas O. Wagner ◽  
Dechao Zhang ◽  
Caroline Poyntner
Keyword(s):  
Ferulic Acid ◽  
Vanillic Acid ◽  
Sole Carbon Source ◽  
Value Added ◽  
Syringic Acid ◽  
High Yield ◽  
Coumaric Acid ◽  
Degradation Study ◽  
Alpine Forest ◽  
Key Points

Abstract Lignin bio-valorization is an emerging field of applied biotechnology and has not yet been studied at low temperatures. Paraburkholderia aromaticivorans AR20-38 was examined for its potential to degrade six selected lignin monomers (syringic acid, p-coumaric acid, 4-hydroxybenzoic acid, ferulic acid, vanillic acid, benzoic acid) from different upper funneling aromatic pathways. The strain degraded four of these compounds at 10°C, 20°C, and 30°C; syringic acid and vanillic acid were not utilized as sole carbon source. The degradation of 5 mM and 10 mM ferulic acid was accompanied by the stable accumulation of high amounts of the value-added product vanillic acid (85–89% molar yield; 760 and 1540 mg l−1, respectively) over the whole temperature range tested. The presence of essential genes required for reactions in the upper funneling pathways was confirmed in the genome. This is the first report on biodegradation of lignin monomers and the stable vanillic acid production at low and moderate temperatures by P. aromaticivorans. Key points • Paraburkholderia aromaticivorans AR20-38 successfully degrades four lignin monomers. • Successful degradation study at low (10°C) and moderate temperatures (20–30°C). • Biotechnological value: high yield of vanillic acid produced from ferulic acid.

scholarly journals Ferulic Acid and Salicylic Acid Foliar Treatments Reduce Short-Term Salt Stress in Chinese Cabbage by Increasing Phenolic Compounds Accumulation and Photosynthetic Performance

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2346
Author(s):  
Ida Linić ◽  
Selma Mlinarić ◽  
Lidija Brkljačić ◽  
Iva Pavlović ◽  
Ana Smolko ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Salicylic Acid ◽  
Salt Stress ◽  
Phenolic Compounds ◽  
Ferulic Acid ◽  
Chinese Cabbage ◽  
Photosynthetic Performance ◽  
Psi Function ◽  
Special Focus ◽  
Short Term

Salinity stress is one of the most damaging abiotic stresses to plants, causing disturbances in physiological, biochemical, and metabolic processes. The exogenous application of natural metabolites is a useful strategy to reduce the adverse effects of stress on crops. We investigated the effect of foliar application of salicylic acid (SA) and ferulic acid (FA) (10–100 μM) on short-term salt-stressed (150 mM NaCl, 72 h) Chinese cabbage plants. Subsequently, proline level, photosynthetic performance, phenolic metabolites with special focus on selected phenolic acids (sinapic acid (SiA), FA, SA), flavonoids (quercetin (QUE), kaempferol (KAE)), and antioxidant activity were investigated in salt-stressed and phenolic acid-treated plants compared with the corresponding controls. Salt stress caused a significant increase in SA and proline contents, a decrease in phenolic compounds, antioxidant activity, and photosynthetic performance, especially due to the impairment of PSI function. SA and FA treatments, with a concentration of 10 μM, had attenuated effects on salt-stressed plants, causing a decrease in proline and SA level, and indicating that the plants suffered less metabolic disturbance. Polyphenolic compounds, especially FA, SiA, KAE, and QUE, were increased in FA and SA treatments in salt-stressed plants. Consequently, antioxidant activities were increased, and photosynthetic performances were improved. FA resulted in a better ameliorative effect on salt stress compared to SA.

scholarly journals Isolation and Identification of Phenolic Compounds from Dianthus orientalis Wildly Grown in Iraq.

2021 ◽  
Vol 30 (2) ◽  
pp. 122-134
Author(s):  
Khansaa Hussein Atiyah ◽  
Enas J Kadhum
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Analytical Techniques ◽  
Extraction Methods ◽  
Coumaric Acid ◽  
Useful Plants ◽  
Isolation And Identification ◽  
Coarse Powder ◽  
Active Constituents ◽  
Whole Plant

The plant Dianthus Orientalis that belongs to the Caryphyllaceae family is one of the useful plants in Iraq. Its seeds are commonly used for toothache. This project provides the first comprehensive research done in Iraq and the world to study the phytochemicals and the methods of extraction and isolation of active constituents from Dianthus orientalis wildly grown in Iraq. The plant was harvested from Penjwin in AL-Sulaymaniyah city, Iraq in September 2019.The whole plant were washed carefully, dried in shade area for two weeks, and milled in a mechanical grinder to a coarse powder. The plant was defatted by maceration with hexane for 7days and dried after that extracted by cold extraction methods using 80% methanol solvent for 9 days then fractionation with chloroform, ethyl acetate and n-butanol to separate the active constituents according to the change in polarities. The chloroform, ethyl acetate fractions were used for identification and isolation of phenolic compounds by TLC, PTLC, HPLC and LC/mass, FTIR. Results of the phytochemical screening exposed the presence of, phenols in the plant extract. The phenolic compound (vanillic acid, coumaric acid, cinnamic acid, genistein, oleuropein) were separated and purified by PTLC. The isolated compounds were subjected to several chemical, chromatographic and spectral analytical techniques for their identification such as TLC, HPLC, FTIR and LC/mass.

Effects of Salinity Stress on Growth and Phenolics of Rice (Oryza sativa L.)

2016 ◽  
Vol 57 ◽  
pp. 1-10 ◽  
Author(s):  
Luong The Minh ◽  
Do Tan Khang ◽  
Pham Thi Thu Ha ◽  
Phung Thi Tuyen ◽  
Truong Ngoc Minh ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ferulic Acid ◽  
Salinity Stress ◽  
Protocatechuic Acid ◽  
Total Phenolics ◽  
Susceptible Cultivar ◽  
Coumaric Acid ◽  
Rice Varieties ◽  
Stress Changes ◽  
Chemical Contents

This study was conducted to determine the correlation between of salinity stress on growth and phenolic compounds in rice. It was observed that salinity stress caused a significant decrease in shoot lengths, fresh and dry weights of all rice varieties. Under salinity stress, changes of chemical contents also differed among phenolic compounds and rice cultivars. Total phenolics and flavonoids, and contents of vanillin and protocatechuic acid in tolerant varieties were strongly increased, whereas in contrast, they were markedly reduced in the susceptible cultivar. Ferulic acid and p-coumaric acid were detected only in tolerance rice. Vanillin and protocatechuic acid may play a role, but ferulic acid and p-coumaric acid may be much involved in the tolerant mechanism against salinity stress. Ferulic acid and p-coumaric acid and their derivatives are able to be exploited as promising agents to reduce detrimental effects of salinity stress on rice production.

Characterization and Classification of Romanian Wines by Origin A chemometric approach based on some metals and phenolic composition

2019 ◽  
Vol 70 (11) ◽  
pp. 3761-3768
Author(s):  
Roxana Elena Ionete ◽  
Diana Ionela Stegarus ◽  
Elisabeta Irina Geana ◽  
Oana Romina Botoran ◽  
Claudia Sandru ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Gallic Acid ◽  
Regional Identity ◽  
Statistical Processing ◽  
Syringic Acid ◽  
Eastern Region ◽  
Coumaric Acid ◽  
Region Of Origin ◽  
Chemometric Approach

There is a growing trend in food industry in combining safety and quality attributes of products with their distinct regional identity. In this study we used data on several elements (Cd, Cu, Cr, Zn, Pb and Ni) and phenolic compounds (gallic acid, syringic acid, p-coumaric acid, (+)-catechin, (-)-epicatechin, trans-resveratrol, rutin, quercetin and vanillin) as complementary markers in the attempt to characterise and differentiate wines from three wine-growing areas (Murfatlar, Recas and Jidvei) of Romania, according to their geographical and varietal origin. Wines from south-eastern region (Murfatlar) were characterized by their lower content in Cu, Cr and Ni, compared with wines from western (Recas) and central (Jidvei) region, while the content of phenolic compounds in the wine samples showed a visible variation, depending both of the grape variety and geographical origin. The statistical processing tools applied to our results allowed robust di�erentiation between regions, wine varieties, and vintage year. The phenolic compounds (gallic acid, (+)-catechin, (-)-epicatechin, rutin, quercetin and resveratrol) showed the best correlations for the varietal discrimination of wines. Using discriminant analysis, a correct classification of the wines by variety was achieved in proportion of 88.89% (based only by phenolic compounds) and 92.59 % (based both on phenolic compounds and metals), revealing that the content of Cu, Cr and Ni is significant for discrimination. Moreover, a 100% successful classification of wines by region of origin was accomplished.

Chemical Composition and Antioxidant Activity of Juices from Mature and Immature Acerola (Malpighia emarginata DC)

2005 ◽  
Vol 11 (4) ◽  
pp. 315-321 ◽  
Author(s):  
A. M. Righetto ◽  
F. M. Netto ◽  
F. Carraro
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Methyl Linoleate ◽  
Vitamin C ◽  
Hplc Analysis ◽  
Syringic Acid ◽  
Coumaric Acid ◽  
Malpighia Emarginata

Chemical composition and antioxidant activity of juice from immature and mature acerola and of concentrated juice from immature acerola were determined. Tartaric, malic and citric acids and a high content of ascorbic acid were found in all the juices. Vitamin C contents were 4.80, 1.90 and 0.97 g/100 g for the concentrated immature, the immature, and the mature acerola juices respectively. The total phenol contents decreased during ripening, from 3.8 mg of catechin/g for immature acerola juice to 1.4 mg of catechin/g for mature acerola juice. The concentrated immature juice had a content of 9.2mg of catechin/g of juice. Catechin, gallic acid, coumaric acid, syringic acid, caffeic acid and ferrulic acid were detected in immature acerola juice by HPLC analysis whereas mature acerola juice showed only one predominant peak with a retention time similar to that of ferrulic acid. The concentrated juice from immature acerola reduced the oxidation of methyl linoleate by 57.2% while the juice from immature acerola reduced the oxidation by 28.1%. These results stated that the antioxidant potential of the acerola juice depended on its content of phenolic compounds and the vitamin C.

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