Three-dimensional tumor spheroid models to investigate tumor-stroma-interactions under the influence of the tumorsuppressor gene ITIH5

2020 ◽  
Author(s):  
S George ◽  
L Jansen ◽  
W Weigand ◽  
IB Runnebaum ◽  
M Dürst ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Tumor Stroma ◽  
Tumor Spheroid

scholarly journals Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation

BMC Biology ◽  
2012 ◽  
Vol 10 (1) ◽  
pp. 29 ◽  
Author(s):  
Maria Vinci ◽  
Sharon Gowan ◽  
Frances Boxall ◽  
Lisa Patterson ◽  
Miriam Zimmermann ◽  
...  
Keyword(s):  
Drug Evaluation ◽  
Three Dimensional ◽  
Tumor Spheroid ◽  
Target Validation ◽  
Functional Assays

scholarly journals Amyloid fibril-based hydrogels for high-throughput tumor spheroid modeling

2020 ◽  
Author(s):  
Namrata Singh ◽  
Komal Patel ◽  
Ambuja Navalkar ◽  
Pradeep Kadu ◽  
Debalina Datta ◽  
...  
Keyword(s):  
Drug Resistance ◽  
High Throughput ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Cancer Therapeutics ◽  
Cost Effective ◽  
Cell Types ◽  
Tumor Spheroid ◽  
Tumor Modeling

AbstractBiomaterials mimicking extracellular matrices (ECM) for three-dimensional (3D) cultures have gained immense interest in tumor modeling and in vitro organ development. Here, we introduce versatile, thixotropic amyloid hydrogels as a bio-mimetic ECM scaffold for 3D cell culture as well as high-throughput tumor spheroid formation using a drop cast method. The unique cross-β-sheet structure, sticky surface, and thixotropicity of amyloid hydrogels allow robust cell adhesion, survival, proliferation, and migration, which are essential for 3D tumor modeling with various cancer cell types. The spheroids formed show overexpression of the signature cancer biomarkers and confer higher drug resistance compared to two-dimensional (2D) monolayer cultures. Using breast tumor tissue from mouse xenograft, we showed that these hydrogels support the formation of tumor spheroids with a well-defined necrotic core, cancer-associated gene expression, higher drug resistance, and tumor heterogeneity reminiscent of the original tumor. Altogether, we have developed a rapid and cost-effective platform for generating in vitro cancer models for the screening of anti-cancer therapeutics and developing personalized medicines.

scholarly journals Three-dimensional dynamics optical coherence tomography for tumor spheroid evaluation

2021 ◽  
Author(s):  
Ibrahim Hossein ◽  
Arata Miyazawa ◽  
Larina Tzu-Wei Shen ◽  
Shuichi Makita ◽  
PRADIPTA MUKHERJEE ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Three Dimensional ◽  
Tumor Spheroid ◽  
Optical Coherence

scholarly journals A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

10.3791/60660 ◽  
2020 ◽  
Author(s):  
Hongwei Shao ◽  
Mecker Moller ◽  
Dazhi Wang ◽  
Albert Ting ◽  
Marcia Boulina ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Tumor Stroma ◽  
Stromal Fibroblast ◽  
Tumor Spheroid

scholarly journals Promising Applications of Tumor Spheroids and Organoids for Personalized Medicine

Cancers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2727 ◽  
Author(s):  
Zarema Gilazieva ◽  
Aleksei Ponomarev ◽  
Catrin Rutland ◽  
Albert Rizvanov ◽  
Valeriya Solovyeva
Keyword(s):  
Personalized Medicine ◽  
Drug Testing ◽  
Tumor Biology ◽  
Three Dimensional ◽  
3D Models ◽  
Therapeutic Agents ◽  
Therapeutic Strategies ◽  
Tumor Spheroid ◽  
Advantages And Disadvantages

One of the promising directions in personalized medicine is the use of three-dimensional (3D) tumor models such as spheroids and organoids. Spheroids and organoids are three-dimensional cultures of tumor cells that can be obtained from patient tissue and, using high-throughput personalized medicine methods, provide a suitable therapy for that patient. These 3D models can be obtained from most types of tumors, which provides opportunities for the creation of biobanks with appropriate patient materials that can be used to screen drugs and facilitate the development of therapeutic agents. It should be noted that the use of spheroids and organoids would expand the understanding of tumor biology and its microenvironment, help develop new in vitro platforms for drug testing and create new therapeutic strategies. In this review, we discuss 3D tumor spheroid and organoid models, their advantages and disadvantages, and evaluate their promising use in personalized medicine.

Development of a three-dimensional microfluidic system for long-term tumor spheroid culture

2012 ◽  
Vol 173 ◽  
pp. 908-913 ◽  
Author(s):  
Karina Ziółkowska ◽  
Radosław Kwapiszewski ◽  
Agnieszka Stelmachowska ◽  
Michał Chudy ◽  
Artur Dybko ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Microfluidic System ◽  
Tumor Spheroid ◽  
Spheroid Culture

scholarly journals A combined 3D printing/CNC micro-milling method to fabricate a large-scale microfluidic device with the small size 3D architectures: an application for tumor spheroid production

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ebrahim Behroodi ◽  
Hamid Latifi ◽  
Zeinab Bagheri ◽  
Esra Ermis ◽  
Shabnam Roshani ◽  
...  
Keyword(s):  
High Resolution ◽  
3D Printing ◽  
Large Scale ◽  
Three Dimensional ◽  
Micro Milling ◽  
Tumor Spheroid ◽  
Microfluidic Chips ◽  
Difficult Time ◽  
Micro Channels ◽  
Milling Method

AbstractThe fabrication of a large-scale microfluidic mold with 3D microstructures for manufacturing of the conical microwell chip using a combined projection micro-stereolithography (PµSL) 3D printing/CNC micro-milling method for tumor spheroid formation is presented. The PµSL technique is known as the most promising method of manufacturing microfluidic chips due to the possibility of creating complex three-dimensional microstructures with high resolution in the range of several micrometers. The purpose of applying the proposed method is to investigate the influence of microwell depths on the formation of tumor spheroids. In the conventional methods, the construction of three-dimensional microstructures and multi-height chips is difficult, time-consuming, and is performed using a multi-step lithography process. Microwell depth is an essential parameter for microwell design since it directly affects the shear stress of the fluid flow and the diffusion of nutrients, respiratory gases, and growth factors. In this study, a chip was made with microwells of different depth varying from 100 to 500 µm. The mold of the microwell section is printed by the lab-made PµSL printer with 6 and 1 µm lateral and vertical resolutions. Other parts of the mold, such as the main chamber and micro-channels, were manufactured using the CNC micro-milling method. Finally, different parts of the master mold were assembled and used for PDMS casting. The proposed technique drastically simplifies the fabrication and rapid prototyping of large-scale microfluidic devices with high-resolution microstructures by combining 3D printing with the CNC micro-milling method.

scholarly journals A 3D Printed Hanging Drop Dripper for Tumor Spheroids Analysis Without Recovery

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Liang Zhao ◽  
Jidong Xiu ◽  
Yang Liu ◽  
Tianye Zhang ◽  
Wenjie Pan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Testing ◽  
Epithelial Mesenchymal Transition ◽  
Three Dimensional ◽  
Tumor Spheroid ◽  
Hanging Drop ◽  
Tumor Spheroids ◽  
Mesenchymal Transition ◽  
Malignant Breast

AbstractCompared with traditional monolayer cell culture, the three-dimensional tumor spheroid has emerged as an essential in vitro model for cancer research due to the recapitulation of the architecture and physiology of solid human tumors. Herein, by implementing the rapid prototyping of a benchtop 3D printer, we developed a new strategy to generate and analyze tumor spheroids on a commonly used multi-well plate. In this method, the printed artifact can be directly mounted on a 96/384-well plate, enables hanging drop-based spheroid formation, avoiding the tedious fabrication process from micromechanical systems. Besides long-term spheroid culture (20 days), this method supports subsequent analysis of tumor spheroid by seamlessly dripping from the printed array, thereby eliminating the need for spheroids retrieval for downstream characterization. We demonstrated several tumor spheroid-based assays, including tumoroid drug testing, metastasis on or inside extracellular matrix gel, and tumor transendothelial (TEM) assay. Based on quantitative phenotypical and molecular analysis without any precarious retrieval and transfer, we found that the malignant breast cancer (MDA-MB-231) cell aggregate presents a more metastatic morphological phenotype than the non-malignant breast cancer (MCF-7) and colonial cancer (HCT-116) cell spheroid, and shows an up-regulation of epithelial-mesenchymal transition (EMT) relevant genes (fold change > 2). Finally, we validated this tumor malignancy by the TEM assay, which could be easily performed using our approach. This methodology could provide a useful workflow for expediting tumoroid modeled in vitro assay, allowing the “Lab-on-a-Cloud” scenario for routine study.

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