scholarly journals Clinical Evaluation of Gingival Thickness and Width according to Dental Arch and Location in Pigmented and Nonpigmented Gingiva

Author(s):  
Divya Saxena ◽  
Rosheni A. Mamen ◽  
Sanjeev Jain ◽  
Gaurav Pandav ◽  
Riddhi Aggarwal ◽  
...  

Abstract Introduction The present study aimed to clinically evaluate and compare the variations in gingival thickness and width of keratinized gingiva according to the dental arch and location in pigmented and nonpigmented gingiva. Materials and Methods A total of 240 sites from systemically healthy subjects were selected and divided into two groups; Group 1(pigmented gingiva) and group 2 (nonpigmented gingiva). After the initial visit of scaling and root planning, the gingival thickness was evaluated mid-buccally in the attached gingiva, that is, the center of the mucogingival junction and free gingival groove, and at the base of the interdental papilla. Similarly, the gingival width was measured as the distance from the gingival margin to the mucogingival junction. A single calibrated examiner recorded the measurements using the digital vernier caliper with a resolution of 0.01 mm. Results Gingival thickness and width were found to be significantly higher in pigmented than nonpigmented gingiva. Also, the maxillary arch was found to have thicker and wider gingiva than the mandibular arch in both the groups. The gingival thickness was found to be maximum in central incisor and minimum in canine in both the groups. However, the width of keratinized gingiva was found to be maximum in lateral incisor in group 1 (pigmented) and central incisor in group 2 (nonpigmented) and minimum in canine in both the groups. Conclusion A significant positive correlation in gingival thickness and width was observed according to dental arch and location in both pigmented and nonpigmented gingiva.

2015 ◽  
Vol 16 (7) ◽  
pp. 537-541
Author(s):  
Claudia Maria Coelho Alves ◽  
Ana Vitoria Imbronito ◽  
Roberto Fraga Moreira Lotufo ◽  
Victor Elias Arana-Chavez

ABSTRACT Aim The aim of the present report was to evaluate the number of melanosomes within keratinocytes on pigmented gingiva, after and before scaling and root planning. Materials and methods Inflamed gingiva biopsies were taken from three patients (group 1). Forty days after scaling and root planning, biopsies were collected from the homologous contralateral areas (group 2). Samples were fixed in 2% glutaraldehyde—2.5% formaldehyde (freshly prepared from paraformaldehyde) in 0.1 M sodium cacodylate buffer, pH 7.4 for 4 hours, and then processed for transmission electron microscopy. Eighty electron micrographs were evaluated for recording the number of granules by a cross-section grid. The granules that were on intersections were recorded as well as the points that appeared on the cytoplasm for calculating the volumetric density (Vd), i.e the volume that the melanosomes occupied into the cytoplasm of keratinocytes. The presence of melanosomes in different stages of maturation and distribution into the cells were recorded with the aid of a magnifying glass. For the statistical analysis, a student t-test was applied. Results Results of the present report showed that melanosomes within keratinocytes were present in a higher number in inflamed gingiva A (11.08 ± 1.47), B (3.16 ± 0.38) and C (4.92 ± 0.89) and decreased after resolving of gingival inflammation A (9.46 ± 0.88), B (1.73 ± 0.25) and C (0.76 ± 0.18). Conclusion There is a possibility that inflammation influences the intensity of gingival melanin pigmentation. Clinical significance The periodontal treatment appears to have an effect on gingival melanin pigmentation. How to cite this article Alves CMC, Imbronito AV, Lotufo RFM, Arana-Chavez VE. Scaling and Root Planning decreases the Number of Melanosomes within Keratinocytes in Human Gingiva: Ultrastructural Analysis of three Cases. J Contemp Dent Pract 2015;16(7):537-541.


2021 ◽  
Vol 11 (2) ◽  
pp. 28-34
Author(s):  
D. A. Grishina ◽  
N. A. Suponeva

Background. The study of the sensitive portion of the medial plantar nerve is relevant not only in the examination of patients with tibial nerve mononeuropathy or its branches, but also in the diagnosis of polyneuropathies.Objective: to analyze the normative parameters of the sensory potential recorded during the study of the medial plantar nerve by the orthodromic method in healthy adults.Materials and methods. 126 sensitive fibers of the medial plantar nerves were studied on the Dantec Keypoint G4 device (Denmark) in 63 healthy individuals (31 men and 32 women; age from 20 to 80 years). 3 groups were identified taking into account age: group 1 included healthy people aged 20 to 39 years (n = 23); group 2 consisted of people aged 40 to 60 years (n = 20); and 3 – older than 60 years (n = 20). The parameters of the sensory potential of the medial plantar nerve are analyzed.Results. The sensory potential in the study of the sensitive portion of the medial plantar nerve was registered in all 126 healthy subjects. Comparative statistical analysis did not demonstrate significant differences between groups 1–3 in the values of such parameters of the sensory potential as the latency of the onset, the duration of the negative phase and the rate of propagation of excitation. At the same time, in groups 2 and 3, the magnitude of the amplitude from peak to peak of the sensory potential was significantly lower compared to group 1, and averaged 8.92 and 7.86 MV, respectively.Conclusion. Knowledge of the regulatory parameters will allow expanding the use of electroneuromyography of the sensitive portion of the medial plantar nerve in clinical and research practice.


1989 ◽  
Vol 35 (11) ◽  
pp. 2169-2172 ◽  
Author(s):  
K Kuroiwa ◽  
S Nakatsuyama ◽  
K Katayama ◽  
T Nagasawa

Abstract We have developed a colorimetric assay for quantifying alpha 2-macroglobulin-trypsin complex (alpha 2M-TRY) in human serum, based on use of a new chromogenic substrate D-gamma-tert-butyloxy-Glu-Gly-Arg-3-carboxy-4-hydroxyanilide dihydrochloride (PS-3001). Within-run CVs by this assay were 4.76%, 1.57%, and 0.83% for trypsin complex concentrations of 3.1, 12.2, and 48.1 U/L, respectively (n = 10 each). Between-day CVs were 5.38%, 3.12%, and 2.20% at each concentration, respectively (n = 7). Mean analytical recoveries of alpha 2M-TRY added to serum were 100%, 105%, and 101% for 9.2, 15.1, and 46.3 U/L, respectively (n = 2). The standard curve obtained was linear up to 330 U/L. We applied this method to the study of alpha 2M-TRY activity in sera from 97 healthy subjects (group 1), from 27 patients with acute pancreatitis (group 2), and from 25 patients with other chylopoietic diseases (group 3); results ranged from 0 to 1.2 U/L (mean = 0.5, SD = 0.3), from 1.2 to 77.4 U/L (mean = 14.6, SD = 19.0), and from 0 to 1.3 U/L (mean = 0.4, SD = 0.3), respectively. Concentrations of enzymatically active alpha 2M-TRY were significantly greater in sera from group 2 than in groups 1 and 3. The determination of serum alpha 2M-TRY activity by this simple, rapid, colorimetric method may be useful for the diagnosis and evaluation of pancreatic disease.


2020 ◽  
Vol 90 (4) ◽  
pp. 500-506
Author(s):  
Alessandro Schwertner ◽  
Renato Rodrigues de Almeida ◽  
Renata Rodrigues de Almeida-Pedrin ◽  
Thais Maria Freire Fernandes ◽  
Paula Oltramari ◽  
...  

ABSTRACT Objective To assess and compare the effects produced in the maxillary dental arch by means of Connecticut intrusion arch (CIA) with or without a cinch back on the distal end of the tube of the first molars. Materials and Methods This study included 44 patients with a mean age of 13.1 ± 1.8 years treated for deep bite with a CIA randomly divided into two groups: group 1 (G1), 22 patients with initial mean age of 12.72 ± 1.74 years treated with the CIA in the upper arch without a cinch back on the distal surface of the tube of the first molars, and group 2 (G2), 22 patients with an initial mean age of 13.67 ± 2.03 years treated with the CIA with a cinch back. Lateral cephalograms were available before treatment (T1) and after intrusion of maxillary incisors (T2). The mean treatment period was 5.5 ± 1.45 months. Intragroup and intergroup changes in the maxillary incisor and molar positions were analyzed by paired and independent t-tests associated with the Holm-Bonferroni correction method for multiple comparisons (P < .05). Results There were significant differences between groups in terms of maxillary incisor displacement. The maxillary incisors flared labially (2.17°) and proclined (1.68 mm) in group 1, whereas a palatal inclination (−1.99°) and retroclination (−1.13 mm) was observed in group 2. No significant differences were found for the molar positions between the groups. Conclusions The presence or absence of a distal bend in CIA affects incisor tipping and proclination during intrusion mechanics.


2007 ◽  
Vol 65 (2b) ◽  
pp. 371-376 ◽  
Author(s):  
Adriana Bastos Conforto ◽  
Fabio Iuji Yamamoto ◽  
Sueli Mieko Oba-Shinjo ◽  
Julio Guy C. Pinto ◽  
Maurício Hoshino ◽  
...  

PURPOSE: It has been suggested that mitochondrial disease may be responsible for a substantial proportion of strokes of indetermined origin. We have preliminarily screened for MELAS (mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes) mutations in young patients with cryptogenic strokes. METHOD: The mitochondrial mutations A3243G and T3271C were investigated in 38 subjects aged less than 46 years. Group 1: 15 patients with cryptogenic strokes; Group 2: 3 patients with diagnosis of MELAS syndrome, including stroke-like episodes; Group 3: 20 healthy subjects. RESULTS: The A3243G mutation was absent in all subjects in Groups 1 and 3 but was present in all subjects in Group 2. CONCLUSION: Our results do not support screening for these mutations to diagnose oligosymptomatic forms of MELAS in cryptogenic strokes in the absence of other features of the syndrome. We suggest that clinical findings should guide mitochondrial genetic testing.


2016 ◽  
Vol 2016 ◽  
pp. 1-6
Author(s):  
Nihat Polat ◽  
Abuzer Gunduz

Purpose.To obtain information about effect of cycloplegia on keratometry and biometry in keratoconus.Methods.48 keratoconus (Group 1) and 52 healthy subjects (Group 2) were included in the study. We measured the flat meridian of the anterior corneal surface (K1), steep meridian of the anterior corneal surface (K2), lens thickness (LT), anterior chamber depth (ACD), and axial length (AL) using the Lenstar LS 900 before and after cycloplegia.Results.The median K1 in Group 1 was 45.64 D before and 45.42 D after cycloplegia, and the difference was statistically significant (P<0.05). The median K2 in Group 1 was 50.96 D before and 50.17 D after cycloplegia, and the difference was significant (P<0.05). The median K1 and K2 in Group 2 were 42.84 and 44.49 D, respectively, before cycloplegia, and 42.84 and 44.56 D after cycloplegia, and the differences were not statistically significant (allP>0.05). There were significant differences in SE, LT, ACD, and RLP between before and after cycloplegia in either Group 1 (allP<0.05) or Group 2 (allP<0.05). There were not statistically significant differences in AL between before cycloplegia and after cycloplegia in either Group 1 (P=0.533) or group 2 (P=0.529).Conclusions.Flattened corneal curvature and increase in ACD following cycloplegia in keratoconus patients were detected.


Author(s):  
Helmut T. Zwahlen ◽  
Thomas Schnell

Daytime conspicuity of fluorescent and non-fluorescent color targets was investigated in the field against a green background in terms of visual detection and recognition, as a function of the peripheral angle and the target size. Two groups of 9 young, healthy subjects each were used. The colors white, blue, green, red, fluorescent red, fluorescent yellow-green, yellow, fluorescent yellow, orange, fluorescent orange were presented for 2 seconds at a radial distance of 30m under the peripheral viewing angles of 10°, 20°, 30° for group 1, and 30°, 40°, 50° for group 2. The target sizes 0.076m x 0.152m, 0.114m x 0.229m, and 0.152m x 0.305m were used in group 1, and 0.114m x 0.229m, 0.152m x 0.305m, and 0.229m x 0.457m were used in group 2. Fluorescent color targets (especially fluorescent yellow-green) were better peripherally detected than the non-fluorescent color targets. White, blue, and green were better recognized but exhibited relatively poor peripheral conspicuity. Therefore it appears that if one wants to maximize the peripheral daytime conspicuity, both highly conspicuous fluorescent colors along with a fairly large target size should be selected. Such a target configuration may for example be needed to attract a driver's attention in situations where a target is located in an observer's visual periphery (peripheral angle > 20°), for example a target approaching a driver at an intersection from a left or right side street.


2016 ◽  
Vol 40 (2) ◽  
pp. 113-117
Author(s):  
Tamer Tuzuner ◽  
Sedanur Turgut ◽  
Bugra Ozen ◽  
Hamiyet Kılınç ◽  
Bora Bagiş

Objective: A fractured whole crown segment can be reattached to its remnant; crowns from extracted teeth may be used as pontics in splinting techniques. We aimed to evaluate the effect of different storage solutions on tooth segment optical properties after different durations. Study design: Sixty central incisor crowns were divided into 6 groups (n = 10); Group 1 was kept dry; Groups 2, 3, 4, 5, and 6 were placed in an isotonic solution, water, milk, saliva, and casein-phosphopeptide–amorphous-calcium-phosphate (CPP–ACP), respectively, for 30 min, 12 h, 1 day, 1 week, and 3 weeks. Color values were measured using a colorimeter. Data were analyzed with Kruskal–Wallis tests, Mann–Whitney U-tests, and Friedman Wilcoxon tests with Bonferroni stepwise corrections (p &lt; 0.05). Results: ΔE* values varied from 0.3 to 15.3 over the 3 week period. Group 1 demonstrated the greatest color changes over all durations; Group 6 exhibited the least. L*, a*, b*, and ΔE* values varied between time periods in all groups; the differences were significant (p &lt; 0.01), except for L* and ΔE* values in Group 2 and a* values in Group 6 (p &gt; 0.01). Comparing ΔE* values, Group 6 was significantly different from the other groups for all durations (p &lt; 0.01), except Group 4. Conclusions: A CPP–ACP complex solution seems a good choice for tooth fragment storage. Milk and saliva solutions may cause perceptible color changes if tooth fragments are stored for 3 weeks before use.


2015 ◽  
Vol 2015 ◽  
pp. 1-4
Author(s):  
Emrullah Beyazyıldız ◽  
Özlem Beyazyıldız ◽  
Süleyman Günaydın ◽  
Emrah Kan ◽  
Mert Şimşek ◽  
...  

Purpose. To evaluate optical low coherence reflectometry (OLCR) parameters in patients with exfoliation syndrome (EXS) undergoing cataract surgery.Methods. Forty-seven eyes of 47 patients with EXS (Group 1), and 55 eyes of 55 healthy subjects (Group 2) were included in the study. Anterior chamber depth (ACD), lens thickness (LT), axial length (AL), central corneal thickness (CCT), horizontal corneal length (HCL), and pupil diameter (PD) parameters were measured by OLCR (Lenstar LS 900, Haag-Streit) and compared between groups. Shapiro-Wilk test and Mann WhitneyUtests were used for statistical analyses.Results. The mean ACD, HCL, and PD values were significantly lower in EXS group than in healthy subjects (P= 0.01,P= 0.04, andP<0.001, resp.). The mean LT was significantly higher in EXS group than in healthy subjects (P= 0.007). There was no significant difference between groups in means of AXL and CCT.Conclusions. According to OLCR measures, eyes with EXS have shallower ACD, smaller PD, thicker LT, shorter HCL, and no significantly different CCT levels.


2008 ◽  
Vol 104 (4) ◽  
pp. 1006-1013 ◽  
Author(s):  
Emeline M. F. Van Craenenbroeck ◽  
Christiaan J. Vrints ◽  
Steven E. Haine ◽  
Katrien Vermeulen ◽  
Inge Goovaerts ◽  
...  

Mobilization of bone marrow-derived endothelial progenitor cells (EPC) might explain exercise-induced improvement of endothelial function. We assessed whether a maximal exercise bout could alter the number of circulating EPC in healthy subjects and whether this effect is related to their cardiovascular risk profile. Additionally, we investigated possible mediators of this effect, namely nitric oxide (NO) bioavailability and vascular endothelial growth factor (VEGF) release. Healthy subjects ( group 1, n = 11; group 2, n = 14) performed a symptom-limited cardiopulmonary exercise test on a bicycle ergometer. Numbers of CD34+/kinase insert domain receptor (KDR)+ cells were determined by flow-cytometric analysis, either after magnetic separation of CD34+ cells ( group 1) or starting from whole blood ( group 2). Serum concentrations of VEGF and NO metabolites were measured by using ELISA. Following exercise, EPC increased by 76% (15.4 ± 10.7 cells/ml vs. 27.2 ± 13.7 cells/ml; P = 0.01) in group 1 and by 69% in group 2 (30.9 ± 14.6 cells/ml vs. 52.5 ± 42.6 cells/ml; P = 0.03). The increase in EPC correlated positively with LDL and total cholesterol/HDL ratio and negatively with peak oxygen consumption and oxygen consumption at anaerobic threshold. VEGF levels increased with exercise, with a strong trend toward significance ( P = 0.055). NO levels remained unchanged. The present study demonstrates that a maximal bout of exercise induces a significant shift in CD34+ cells toward CD34+/KDR+ cells. This response was larger in subjects with a less favorable lipid profile.


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