scholarly journals Scaling and Root Planning decreases the Number of Melanosomes within Keratinocytes in Human Gingiva: Ultrastructural Analysis of Three Cases

2015 ◽  
Vol 16 (7) ◽  
pp. 537-541
Author(s):  
Claudia Maria Coelho Alves ◽  
Ana Vitoria Imbronito ◽  
Roberto Fraga Moreira Lotufo ◽  
Victor Elias Arana-Chavez
Keyword(s):  
Present Report ◽  
Ultrastructural Analysis ◽  
Melanin Pigmentation ◽  
Transmission Electron ◽  
Cacodylate Buffer ◽  
Human Gingiva ◽  
Group 2 ◽  
Root Planning ◽  
Scaling And Root Planning ◽  
Group 1

ABSTRACT Aim The aim of the present report was to evaluate the number of melanosomes within keratinocytes on pigmented gingiva, after and before scaling and root planning. Materials and methods Inflamed gingiva biopsies were taken from three patients (group 1). Forty days after scaling and root planning, biopsies were collected from the homologous contralateral areas (group 2). Samples were fixed in 2% glutaraldehyde—2.5% formaldehyde (freshly prepared from paraformaldehyde) in 0.1 M sodium cacodylate buffer, pH 7.4 for 4 hours, and then processed for transmission electron microscopy. Eighty electron micrographs were evaluated for recording the number of granules by a cross-section grid. The granules that were on intersections were recorded as well as the points that appeared on the cytoplasm for calculating the volumetric density (Vd), i.e the volume that the melanosomes occupied into the cytoplasm of keratinocytes. The presence of melanosomes in different stages of maturation and distribution into the cells were recorded with the aid of a magnifying glass. For the statistical analysis, a student t-test was applied. Results Results of the present report showed that melanosomes within keratinocytes were present in a higher number in inflamed gingiva A (11.08 ± 1.47), B (3.16 ± 0.38) and C (4.92 ± 0.89) and decreased after resolving of gingival inflammation A (9.46 ± 0.88), B (1.73 ± 0.25) and C (0.76 ± 0.18). Conclusion There is a possibility that inflammation influences the intensity of gingival melanin pigmentation. Clinical significance The periodontal treatment appears to have an effect on gingival melanin pigmentation. How to cite this article Alves CMC, Imbronito AV, Lotufo RFM, Arana-Chavez VE. Scaling and Root Planning decreases the Number of Melanosomes within Keratinocytes in Human Gingiva: Ultrastructural Analysis of three Cases. J Contemp Dent Pract 2015;16(7):537-541.

scholarly journals Clinical Evaluation of Gingival Thickness and Width according to Dental Arch and Location in Pigmented and Nonpigmented Gingiva

2020 ◽  
Author(s):  
Divya Saxena ◽  
Rosheni A. Mamen ◽  
Sanjeev Jain ◽  
Gaurav Pandav ◽  
Riddhi Aggarwal ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Dental Arch ◽  
Central Incisor ◽  
Gingival Thickness ◽  
Attached Gingiva ◽  
Group 2 ◽  
Interdental Papilla ◽  
Root Planning ◽  
Scaling And Root Planning ◽  
Group 1

Abstract Introduction The present study aimed to clinically evaluate and compare the variations in gingival thickness and width of keratinized gingiva according to the dental arch and location in pigmented and nonpigmented gingiva. Materials and Methods A total of 240 sites from systemically healthy subjects were selected and divided into two groups; Group 1(pigmented gingiva) and group 2 (nonpigmented gingiva). After the initial visit of scaling and root planning, the gingival thickness was evaluated mid-buccally in the attached gingiva, that is, the center of the mucogingival junction and free gingival groove, and at the base of the interdental papilla. Similarly, the gingival width was measured as the distance from the gingival margin to the mucogingival junction. A single calibrated examiner recorded the measurements using the digital vernier caliper with a resolution of 0.01 mm. Results Gingival thickness and width were found to be significantly higher in pigmented than nonpigmented gingiva. Also, the maxillary arch was found to have thicker and wider gingiva than the mandibular arch in both the groups. The gingival thickness was found to be maximum in central incisor and minimum in canine in both the groups. However, the width of keratinized gingiva was found to be maximum in lateral incisor in group 1 (pigmented) and central incisor in group 2 (nonpigmented) and minimum in canine in both the groups. Conclusion A significant positive correlation in gingival thickness and width was observed according to dental arch and location in both pigmented and nonpigmented gingiva.

scholarly journals Endothelium-derived hyperpolarizing factor in preeclampsia: heterogeneous contribution, mechanisms, and morphological prerequisites

2008 ◽  
Vol 294 (2) ◽  
pp. R510-R519 ◽  
Author(s):  
Leanid Luksha ◽  
Henry Nisell ◽  
Natallia Luksha ◽  
Marius Kublickas ◽  
Kjell Hultenby ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Subcutaneous Fat ◽  
Normal Pregnancy ◽  
Transmission Electron ◽  
Main Pathway ◽  
Before And After ◽  
Group 2 ◽  
Cytochrome P 450 ◽  
Group 1

We hypothesized that in preeclampsia (PE), contribution of endothelium-derived hyperpolarizing factor (EDHF) and the mechanism/s of its action differ from that in normal pregnancy (NP). We aimed to assess endothelial function and morphology in arteries from NP and PE with particular focus on EDHF. Arteries (≈200 μm) were dissected from subcutaneous fat biopsies obtained from women undergoing cesarean section. With the use of wire myography, responses to the endothelium-dependent agonist bradykinin (BK) were determined before and after inhibition of pathways relevant to EDHF activity. The overall responses to BK in arteries from PE ( n = 13) and NP ( n = 17) were similar. However, in PE, EDHF-mediated relaxation was reduced ( P < 0.05). All women within the PE group were divided into two subgroups: with more ( group 1) or less ( group 2) than 50% reduction of EDHF-typed responses after 18-α-glycyrrhetinic acid (an inhibitor of myoendothelial gap junctions, MEGJs). The division showed that 1) MEGJs are principally involved when the EDHF contribution is reduced; and 2) when the EDHF contribution is similar to that in NP, the H2O2 and/or cytochrome P-450 epoxygenase products of arachidonic acid (AA), along with MEGJs, confer EDHF-mediated relaxation. In contrast, MEGJs were the main pathway for EDHF in NP. The abundant presence of MEGJs in arteries from NP but deficiency of them in PE was observed using transmission electron microscopy. We conclude that PE is associated with heterogeneous contribution of EDHF, and the mechanism behind EDHF-typed responses is mediated either by MEGJs alone or in combination with H2O2 or cytochrome P-450 epoxygenase metabolites of AA.

Evaluating the role of extracellular vesicles as a biomarker under transmission electron microscope in prostate cancer and benign prostate hyperplasia patients

2021 ◽  
pp. 039156032110186
Author(s):  
Vibhore Agarwal ◽  
Sher Singh Yadav ◽  
Sanjay Kumar ◽  
Nishkarsh Mehta ◽  
Gagandeep Talwar ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Extracellular Vesicles ◽  
Gleason Score ◽  
Transmission Electron Microscope ◽  
Cell Of Origin ◽  
Prostate Hyperplasia ◽  
Transmission Electron ◽  
The Mean ◽  
Group 2 ◽  
Group 1

Objectives: To our knowledge, EVs (extracellular vesicles) are heterogenous encapsulated nanoparticles generated by the biological cells. EVs can be found in blood, urine and tissue of origin. They contain DNAs, RNAs, proteins specific to the cell of origin. It has been found that in PCa, increase in number of EVs can modulate phenotype and function of the recipient cells. Methods: This prospective randomized double-blind pilot study was conducted in the SMS Medical College, Jaipur in collaboration with All India Institute of Medical Sciences, New Delhi. For morphometric analysis, the number of extracellular vesicles per micrograph were counted under transmission electron microscope. Results: Out of 16 patients taken in our study, six were in group 1 (BPH group) and 10 were in group 2 (PCa group). The mean number of EVs was significantly higher in the cells of group 2 in comparison to the group 1. Among the PCa patients, mean number of EVs were 25, 30, 35, 43, 46 for the Gleason score 6, 7, 8, 9, 10 respectively. In our study the mean number of EVs in the newly diagnosed PCa group was less as compared to the CSPC and CRPC group. Conclusions: EVs are membrane bound particles shed regularly from the cells in the extracellular milieu under normal physiological and pathological conditions. In our study the number of EVs were more in the PCa cells in comparison with the BPH cells and among the PCa cells they bear a positive correlation with the Gleason score, thus EVs have the potential to become a biomarker.

scholarly journals The protective effects of erythropoietin on photoreceptor damage by formaldehyde

2016 ◽  
Vol 8 (1) ◽  
pp. 10-17 ◽  
Author(s):  
Mehdi Mard Khoshnood ◽  
Mohammad Zareie ◽  
Ali Amiri ◽  
Ali Zarenezhad ◽  
Elham Zarenezhad ◽  
...  
Keyword(s):  
Outer Segment ◽  
Outer Nuclear Layer ◽  
Control Group ◽  
Ischemic Damage ◽  
Protective Effects ◽  
Adult Rats ◽  
Photoreceptor Layer ◽  
Transmission Electron ◽  
Group 2 ◽  
Group 1

Introduction: The photoreceptor layer of retina has important role for sight. The previous study showed that accidental formaldehyde injection caused ischemia and damage in the retina. On the other hand the erythropoietin prevents neuronal injury of ischemic damage. Objectives: The aim of this study was to survey the effect of erythropoietin on retro bulbar formaldehyde injected photoreceptor layer of rat retina. Materials and methods: 30 adult rats were used and divided into three groups: 1- control group, 2- formaldehyde group (1 ml retro bulbar injected by 10% formaldehyde solution), 3- erythropoietin group (5000 units/kg immediately intraperitoneally injected by erythropoietin after formaldehyde injection for 7 days). The photoreceptor layer of retina studied using a transmission electron microscope. Results: Our observation showed that disorganization and vacuolization in outer segment and inner segment, pyknotic and karyolysis in outer nuclear layer were seen in formaldehyde group. But the minor sign of pathology such as lightly vacuolization in inner segment were obvious in erythropoietin group. Conclusion: We concluded that formaldehyde caused damage in photoreceptor layer and erythropoietin was improvement this injury. Nepal J Ophthalmol 2016; 8(15): 10-17

scholarly journals A Comparative Clinical Study on Surgical Blade and Diode Laser in the Treatment of Gingival Melanin Pigmentation

2021 ◽  
Vol 10 (10) ◽  
pp. 689-693
Author(s):  
Somit Kumar Jain ◽  
Nina Shenoy ◽  
Mehul Kumar Chourasia ◽  
Amitha Ramesh
Keyword(s):  
Wound Healing ◽  
Clinical Study ◽  
Diode Laser ◽  
Pain Perception ◽  
Rank Test ◽  
Side Group ◽  
Melanin Pigmentation ◽  
Comparative Clinical Study ◽  
Group 2 ◽  
Group 1

BACKGROUND Gingival melanin pigmentation (GMP) is a common aesthetic concern. The gold standard for depigmentation, is surgery. Recently soft tissue lasers have gained popularity. The purpose of this study was to compare and evaluate the clinical efficacy of surgical blade and laser treatment in terms of pain perception and wound healing outcomes, 7 and 14 days following the procedures for the treatment of gingival depigmentation. METHODS This 14 day follow up comparative clinical study, utilized a split mouth design, in the anterior region. Fifteen patients with moderate to heavy bilateral gingival pigmentation were included as per the criteria by Dummett et al. The patients’ right side (group 1) was treated with surgical blade and the left side (group 2) with diode laser (Picasso, AMD LASERS™) by a trained clinician. Pain perception was assessed using Visual Analog Scale (VAS) and wound healing was assessed using clinical parameters. RESULTS Data was analysed using paired t test and Wilcoxon’s signed rank test. P value of < 0.05 was considered statistically significant. On day 7, more than 70 % patients had slight pain in group 1. Whereas, 47 % experienced no pain in group 2. The mean difference in VAS scores between the groups at day 7, was statistically significant. No statistically significant distinction in wound healing was observed 7 days following both procedures. No pain, and complete epithelialisation was noted in both groups on day 14. CONCLUSIONS Pain experienced by the patients was slightly more in the scalpel group compared to laser. There was no difference in wound healing between both groups. KEY WORDS GMP, Gingival Melanin Pigmentation, VAS Scale, Depigmentation, Wound Healing, Laser, Surgical Blade, Split Mouth Design

Effect of Contraceptive Steroids on the Endometrium of Guinea Pig: SEM study

1977 ◽  
Vol 35 ◽  
pp. 668-669
Author(s):  
Mai M. Said ◽  
Ramesh K. Nayak ◽  
Randall E. McCoy
Keyword(s):  
Guinea Pig ◽  
Reproductive Tract ◽  
Three Dimensional ◽  
Female Reproductive Tract ◽  
Human Female ◽  
Surface Ultrastructure ◽  
Transmission Electron ◽  
Sem Study ◽  
Uterine Mucosa ◽  
Group 1

Burgos and Wislocki described changes in the mucosa of the guinea pig uterus, cervix and vagina during the estrous cycle investigated by transmission electron microscopy. More recently, Moghissi and Reame reported the effects of progestational agents on the human female reproductive tract. They found drooping and shortening of cilia in norgestrel and norethindrone- treated endometria. To the best of our knowledge, no studies concerning the effects of mestranol and norethindrone given concurrently on the three-dimensional surface features on the uterine mucosa of the guinea pig have been reported. The purpose of this study was to determine the effect of mestranol and norethindrone on surface ultrastructure of guinea pig uterus by SEM.Seventy eight animals were used in this study. They were allocated into two groups. Group 1 (20 animals) was injected intramuscularly 0.1 ml vegetable oil and served as controls.

Hexachlorobenzene toxicity in the monkey ovary: III. Ultrastructure induced by high (10 mg/kg) dose exposure

1991 ◽  
Vol 49 ◽  
pp. 130-131
Author(s):  
A. Singh ◽  
A. Dykeman ◽  
J. Jarrelf ◽  
D. C. Villeneuve
Keyword(s):  
Present Report ◽  
Reproductive Toxicity ◽  
Control Group ◽  
Primate Model ◽  
Human Follicular Fluid ◽  
Thin Sections ◽  
Cynomolgus Monkeys ◽  
Cacodylate Buffer ◽  
Induction Cycle ◽  
Comprehensive Study

Hexachlorobenzene (HCB), a persistent and mobile organochlorine pesticide, occurs in environment. HCB has been shown to be present in human follicular fluid. An objective of the present report, which is part of a comprehensive study on reproductive toxicity of HCB, was to determine the cytologic effects of the compound on ovarian follicles in a primate model.Materials and Methods. Eight Cynomolgus monkeys were housed under controlled conditions at Animal facility of Health and Welfare, Ottawa. Animals were orally administered gelatin capsules containing HCB mixed with glucose in daily dosages of 0.0 or 10 mg/kg b.w. for 90 days; the former was the control group. On the menstrual period following completion of dosing, the monkeys underwent an induction cycle of superovulation. At necropsy, one-half of an ovary from each animal was diced into ca. 2- to 3-mm cubed specimens that were fixed by immersion in 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.3). Subsequent procedures followed to obtain thin sections that were examined in a Hitachi H-7000 electron microscope have been described earlier.

TEM comparison of osmium vs osmium with potassium ferricyanide secondary fixatives and the impact of secondary fixative temperature on tissue preservation/contrast quality

1996 ◽  
Vol 54 ◽  
pp. 910-911
Author(s):  
J. W. Horn ◽  
B. J. Dovey-Hartman ◽  
V. P. Meador
Keyword(s):  
Osmium Tetroxide ◽  
Potassium Ferricyanide ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Microscopic Evaluation ◽  
Cacodylate Buffer ◽  
Transmission Electron Microscopic ◽  
Glutaraldehyde Solution ◽  
Temperature Impact ◽  
The Impact

Osmium tetroxide (OsO4) is a universally used secondary fixative for routine transmission electron microscopic evaluation of biological specimens. Use of OsO4 results in good ultrastructural preservation and electron density but several factors, such as concentration, length of exposure, and temperature, impact overall results. Potassium ferricyanide, an additive used primarily in combination with OsO4, has mainly been used to enhance the contrast of lipids, glycogen, cell membranes, and membranous organelles. The purpose of this project was to compare the secondary fixative solutions, OsO4 vs. OsO4 with potassium ferricyanide, and secondary fixative temperature for determining which combination gives optimal ultrastructural fixation and enhanced organelle staining/contrast.Fresh rat liver samples were diced to ∼1 mm3 blocks, placed into porous processing capsules/baskets, preserved in buffered 2% formaldehyde/2.5% glutaraldehyde solution, and rinsed with 0.12 M cacodylate buffer (pH 7.2). Tissue processing capsules were separated (3 capsules/secondary fixative.solution) and secondarily fixed (table) for 90 minutes. Tissues were buffer rinsed, dehydrated with ascending concentrations of ethanol solutions, infiltrated, and embedded in epoxy resin.

Ultrastructural Immunocytochemistry of Five Rat Pituitary Adenomas

1980 ◽  
Vol 38 ◽  
pp. 724-725
Author(s):  
D. J. McComb ◽  
N. Ryan ◽  
E. Horvath ◽  
K. Kovacs ◽  
E. Nagy ◽  
...  
Keyword(s):  
Pituitary Adenomas ◽  
Pituitary Tumors ◽  
Electron Microscopic ◽  
Electron Microscopic Immunocytochemistry ◽  
Transmission Electron ◽  
Rat Pituitary ◽  
Radiation Induced ◽  
Group 5 ◽  
Group 2 ◽  
Microscopic Techniques

Conventional light and electron microscopic techniques failed to clarify the cellular composition and derivation of spontaneous and induced, intrasellar and transplanted pituitary adenomas in rats (1). In the present work, electron microscopic immunocytochemistry was applied to evaluate five adenohypo-physial tumors using a technique described by Moriarty and Garner (2). Spontaneously occurring pituitary adenomas (group 1) were harvested from aging female Long-Evans rats. R-Amsterdam rats were treated with 2 x 1.0 mg estrone acetate (HogivaI) s.c. weekly for 6 months. Pituitary adenomas in excess of 30 mg were removed from these animals to make up the tumors of group 2. Groups 3 and 4 consisted of estrogen-induced autonomous transplan¬ted pituitary tumors MtT.WlO and MtT.F4. Group 5 was a radiation-induced transplanted autonomous pituitary tumor MtT.W5. The tumors of groups 3,4 and 5 were allowed to proliferate in host rats 6-8 weeks prior to removal for processing. Tissue was processed for transmission electron microscopy (glutaraldehyde fixation, OsO4 postfixation and epoxy resin embedding), and electron microscopic immunocytochemistry (3% paraformaldehyde fixation and Araldite embedding).

In-vivo evaluation of the effect of cyanoacrylate on prosthetic vascular graft infection – does cyanoacrylate increase the severity of infection?

VASA ◽  
2020 ◽  
Vol 49 (4) ◽  
pp. 281-284
Author(s):  
Atıf Yolgosteren ◽  
Gencehan Kumtepe ◽  
Melda Payaslioglu ◽  
Cuneyt Ozakin
Keyword(s):  
Vascular Graft ◽  
Graft Infection ◽  
Vascular Graft Infection ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Prosthetic Vascular Graft Infection ◽  
Group 1

Summary. Background: Prosthetic vascular graft infection (PVGI) is a complication with high mortality. Cyanoacrylate (CA) is an adhesive which has been used in a number of surgical procedures. In this in-vivo study, we aimed to evaluate the relationship between PVGI and CA. Materials and methods: Thirty-two rats were equally divided into four groups. Pouch was formed on back of rats until deep fascia. In group 1, vascular graft with polyethyleneterephthalate (PET) was placed into pouch. In group 2, MRSA strain with a density of 1 ml 0.5 MacFarland was injected into pouch. In group 3, 1 cm 2 vascular graft with PET piece was placed into pouch and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. In group 4, 1 cm 2 vascular graft with PET piece impregnated with N-butyl cyanoacrylate-based adhesive was placed and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. All rats were scarified in 96th hour, culture samples were taken where intervention was performed and were evaluated microbiologically. Bacteria reproducing in each group were numerically evaluated based on colony-forming unit (CFU/ml) and compared by taking their average. Results: MRSA reproduction of 0 CFU/ml in group 1, of 1410 CFU/ml in group 2, of 180 200 CFU/ml in group 3 and of 625 300 CFU/ml in group 4 was present. A statistically significant difference was present between group 1 and group 4 (p < 0.01), between group 2 and group 4 (p < 0.01), between group 3 and group 4 (p < 0.05). In terms of reproduction, no statistically significant difference was found in group 1, group 2, group 3 in themselves. Conclusions: We observed that the rate of infection increased in the cyanoacyrylate group where cyanoacrylate was used. We think that surgeon should be more careful in using CA in vascular surgery.

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