HPLC Separation and Quantitative Determination of Ginsenosides from Panax ginseng, Panax quinquefolium and from Ginseng Drug Preparations

Abstract Allium sativum L (garlic) is an essential component of many polyherbal oils used in traditional systems of medicine. Allyl disulfide has been a major component found in vegetable oil macerate of garlic, and can be used as reliable marker for determination of garlic in oil macerates of garlic. The HPLC separation of allyl disulfide was achieved on a Phenomenex Luna C18 (25 cm × 4.6 mm id × 5 μm particle size) column using acetonitrile–water–tetrahydrofuran (70 + 27 + 3, v/v/v) mobile phase at a flow rate of 1.0 mL/min. Quantitation was achieved with UV detection at 298 nm over the concentration range 8–48 μg/mL. HPTLC separation of allyl disulfide was achieved on an aluminum-backed layer of silica gel 60 F254 using n-hexane mobile phase. Quantitation was achieved by densitometric analysis at 298 nm over the 200–1200 ng/band concentration range. The methods were validated according to International Conference on Harmonization guidelines.

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HPLC Separation and Quantitative Determination of Cardenolides in Herba Convallariae

Planta Medica ◽

10.1055/s-2006-961702 ◽

1992 ◽

Vol 58 (S 1) ◽

pp. 682-684

Author(s):

L. Krenn ◽

L. Schlifelner ◽

T. Stimpfl ◽

B. Kopp

Keyword(s):

Quantitative Determination ◽

Hplc Separation

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Quantitative determination of ginsenosides from Panax ginseng roots and ginseng preparations by thin layer chromatography–densitometry

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/s0731-7085(99)00109-0 ◽

1999 ◽

Vol 21 (1) ◽

pp. 187-192 ◽

Cited By ~ 45

Author(s):

J Corthout ◽

T Naessens ◽

S Apers ◽

A.J Vlietinck

Keyword(s):

Thin Layer ◽

Quantitative Determination ◽

Thin Layer Chromatography ◽

Panax Ginseng ◽

Layer Chromatography

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Development of a SPE-HPLC-PDA Method for the Determination of Tetracyclines in Soils

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.675-677.288 ◽

2014 ◽

Vol 675-677 ◽

pp. 288-294 ◽

Cited By ~ 2

Author(s):

Bao Li Sun ◽

Yi Wei Dong ◽

Hong Shan ◽

Jin Li Huang ◽

Cheng Feng Tong

Keyword(s):

Standard Deviation ◽

Quantitative Determination ◽

Extraction Efficiency ◽

Soil Samples ◽

Hplc Separation ◽

Buffer Solution ◽

Soil Extracts ◽

Relative Standard ◽

Chromatographic Condition

A simple and rapid SPE-HPLC/PDA method has been developed for the quantitative determination of tetracyclines in soils. An ultrasonic extraction system for the soil samples was confirmed as a combination of a 1:2 ratio of EDTA-McIlvaine buffer solution with methanol by comparing the extraction efficiency of 4 solvents and optimising the combination ratio. The soil extracts were purified and concentrated by Oasis-MAX cartridge followed by HPLC separation and PDA detection. The optimal chromatographic condition effectively separated the object and interfering substance, which can improve the sensitivity of the instrument. The limits of detection were lower than 20 μg·kg-1, and the limits of quantification were no more than 60 μg·kg-1 for all compounds in the soil. The recoveries of tetracyclines peaked at levels of 50-500 μg·kg-1 and averaged at 62.1% to 108.9% with relative standard deviation values of less than 5%. This method was applied to analyse real soil samples from suburban Beijing farmland, and TC and OTC residues were detected in the soil.

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Quantitative Determination of Ecdysteroids in Sida rhombifolia L. and various other Sida Species Using LC-UV, and their Anatomical Characterization

Natural Product Communications ◽

10.1177/1934578x0800300508 ◽

2008 ◽

Vol 3 (5) ◽

pp. 1934578X0800300 ◽

Cited By ~ 1

Author(s):

Bharathi Avula ◽

Vaishali Joshi ◽

Yan-Hong Wang ◽

Atul N. Jadhav ◽

Ikhlas A. Khan

Keyword(s):

Acetic Acid ◽

Quantitative Determination ◽

Reversed Phase ◽

Morphological Description ◽

Hplc Separation ◽

Aerial Parts ◽

Acetic Acid Water ◽

Sida Rhombifolia ◽

Phase Column

The genus Sida is used extensively in herbal medicine. A simple and specific analytical method for the quantitative determination of ecdysteroids in S. rhombifolia is reported. The ecdysteroids were separated with an acetonitrile (0.1% acetic acid)-water (0.1% acetic acid) gradient at a flow rate of 1.0 mL per minute. The HPLC separation was performed on a Synergi Polar-RP reversed phase column and operated at 35°C. Detection was performed at 247 nm. The method facilitated the quantification of seven ecdysteroids [20-hydroxyecdysone-3- O-β-D-glucopyranoside (1), 20-hydroxyecdysone (2), 2-deoxy ecdysone-3- O-β-D-glucopyranoside (3), pterosterone-3- O-β-D-glucopyranoside (4), ecdysone-3- O-β-D-glucopyranoside (5), ecdysone (6), and 20-hydroxy-(25-acetyl)-ecdysone-3- O-β-D-glucopyranoside (7)] in aerial parts of S. rhombifolia. The LOD and LOQ for compounds 1–7 were found to be in the range from 0.07-0.1 μg/mL and 0.3–0.5 μg/mL, respectively. In the present study, a micro-morphological description of S. rhombifolia is provided. The developed method was used for the determination of ecdysteroids in various other species of Sida, which were compared both micro-morphologically and chemically.

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