Effect of quantum tunneling on single strand breaks in a modeled gas phase cytidine nucleotide induced by low energy electron: A theoretical approach

2013 ◽  
Vol 139 (4) ◽  
pp. 045103 ◽  
Author(s):  
Renjith Bhaskaran ◽  
Manabendra Sarma
Keyword(s):  
Gas Phase ◽  
Theoretical Approach ◽  
Quantum Tunneling ◽  
Single Strand ◽  
Low Energy ◽  
Energy Electron ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Low Energy Electron

Vacuum‐UV and Low‐Energy Electron‐Induced DNA Strand Breaks – Influence of the DNA Sequence and Substrate

ChemPhysChem ◽  
2019 ◽  
Vol 20 (6) ◽  
pp. 823-830 ◽  
Author(s):  
Stefanie Vogel ◽  
Kenny Ebel ◽  
Robin M. Schürmann ◽  
Christian Heck ◽  
Till Meiling ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Dna Strand Breaks ◽  
Low Energy ◽  
Energy Electron ◽  
Strand Breaks ◽  
Low Energy Electron ◽  
Dna Strand ◽  
Vacuum Uv

Kinetics of low energy electron attachment to some fluorinated alcohols in the gas phase

2014 ◽  
Vol 591 ◽  
pp. 282-286 ◽  
Author(s):  
K. Wnorowski ◽  
J. Wnorowska ◽  
J. Kopyra ◽  
B. Michalczuk ◽  
I. Szamrej ◽  
...  
Keyword(s):  
Gas Phase ◽  
Electron Attachment ◽  
Low Energy ◽  
Energy Electron ◽  
Fluorinated Alcohols ◽  
Low Energy Electron ◽  
Kinetics Of

scholarly journals Low-energy electron holography imaging of conformational variability of single-antibody molecules from electrospray ion beam deposition

2021 ◽  
Vol 118 (51) ◽  
pp. e2112651118
Author(s):  
Hannah Ochner ◽  
Sven Szilagyi ◽  
Sabine Abb ◽  
Joseph Gault ◽  
Carol V. Robinson ◽  
...  
Keyword(s):  
Gas Phase ◽  
Ion Beam ◽  
Protein Structures ◽  
Low Energy ◽  
Energy Electron ◽  
Single Layer Graphene ◽  
Conformational Variability ◽  
Ion Beam Deposition ◽  
Low Energy Electron ◽  
Beam Deposition

Imaging of proteins at the single-molecule level can reveal conformational variability, which is essential for the understanding of biomolecules. To this end, a biologically relevant state of the sample must be retained during both sample preparation and imaging. Native electrospray ionization (ESI) can transfer even the largest protein complexes into the gas phase while preserving their stoichiometry and overall shape. High-resolution imaging of protein structures following native ESI is thus of fundamental interest for establishing the relation between gas phase and solution structure. Taking advantage of low-energy electron holography’s (LEEH) unique capability of imaging individual proteins with subnanometer resolution, we investigate the conformational flexibility of Herceptin, a monoclonal IgG antibody, deposited by native electrospray mass-selected ion beam deposition (ES-IBD) on graphene. Images reconstructed from holograms reveal a large variety of conformers. Some of these conformations can be mapped to the crystallographic structure of IgG, while others suggest that a compact, gas-phase–related conformation, adopted by the molecules during ES-IBD, is retained. We can steer the ratio of those two types of conformations by changing the landing energy of the protein on the single-layer graphene surface. Overall, we show that LEEH can elucidate the conformational heterogeneity of inherently flexible proteins, exemplified here by IgG antibodies, and thereby distinguish gas-phase collapse from rearrangement on surfaces.

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