Isolation and identification of sulfate reducing bacteria (SRB) from the sediment pond after a coal mine in Samarinda, East Kalimantan

2017 ◽  
Author(s):  
Eko Kusumawati ◽  
Sudrajat ◽  
Junita Susilaning Putri
Keyword(s):  
Coal Mine ◽  
Sulfate Reducing Bacteria ◽  
Isolation And Identification ◽  
Sulfate Reducing ◽  
East Kalimantan ◽  
Reducing Bacteria

scholarly journals Short Communication: The potential of Sulfate Reducing Bacteria of ex-coal mine sediment pond as sulfate reducing agents of acid land in Samarinda, Indonesia

2017 ◽  
Vol 7 (2) ◽  
pp. 79-82
Author(s):  
EKO KUSUMAWATI ◽  
SUDRAJAT SUDRAJAT ◽  
IKA PURNAMASARI ◽  
BINA CRISTYANTI PANGGABEAN ◽  
MAIDA APRIYANTI
Keyword(s):  
Sulfate Reduction ◽  
Coal Mine ◽  
Short Communication ◽  
Acid Soils ◽  
Sulfate Reducing Bacteria ◽  
Reducing Agents ◽  
Sulfate Reducing ◽  
East Kalimantan ◽  
Reduction Efficiency ◽  
Reducing Bacteria

Kusumawati E, Sudrajat, Purnamasari I, Panggabean BC, Apriyanti M. 2017. Short Communication: The potential of Sulfate Reducing Bacteria of ex-coal mine sediment pond as sulfate reducing agents of acid land in Samarinda, Indonesia. Bonorowo Wetlands 1: 79-82. The study aims to determine the effect of pH medium on the growth of sulfate reducing bacteria taken from the e-coal mine sediment pond and to determine its potential as a reducing sulphate agent of acid ex-coal mine land in Samarinda, East Kalimantan, Indonesia. Six SRB isolated from ex-coal mine sediment pond in Samarinda were used in this study. The SRB potency test in reducing sulfate was conducted by growing the SRB on Postgate liquid medium at different pH of 2, 4 and 6 by the addition of acid soils on each treatment. The results showed that sulfate reducing bacteria isolated from ex-coal mine sediment pond in Samarinda,, i.e., sp.1 (Desulfococcus sp.), sp.2 (Desulfotomaculum sp.), sp.3 (Desulfobacter sp.), sp.4 (Desulfobulbus sp. ), sp.5 (Desulfobacterium sp.) and sp.6 (Desulfotomaculum sp.) had potential as sulfate reducing agent of acid land. The efficiency of sulfate reduction was 89%, 91% and 91% in the pH of 2, 4 and 6, respectively. This indicated that the highest number of sulfate reduction is in the medium with pH 4 and pH 6. In addition, sp.5 (Desulfobacterium sp.) growing on medium at pH 4 had the best sulfate reduction efficiency (93%) compared with other SRB isolates.

scholarly journals BIOTEKNOLOGI LINGKUNGAN UNTUK PENANGGULANGAN LIMBAH MENGANDUNG KROM

2008 ◽  
Vol 10 (2) ◽  
pp. 50
Author(s):  
Dwi Andreas Santosa ◽  
Tommy Kurniawan ◽  
F. Dian Lina
Keyword(s):  
Laboratory Experiment ◽  
Incubation Period ◽  
Oil And Gas ◽  
Sulfate Reducing Bacteria ◽  
Sulphate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Cr Content ◽  
East Kalimantan ◽  
Metal Plating ◽  
Reducing Bacteria

<p>The biotechnological approaches for treatment of wastewater are widely studied within the last two decades. We show that this approach can be used for treatment of Cr-contaminated wastewater. The sulfate reducing bacteria Desulfotomaculum orientis ICBB 1204 is used in a laboratory experiment to treat Cr-contained wastewater from metal plating industry and the oil and gas company, PT VICO, East Kalimantan. After adaption of the bacteria for around 10 days in the wastewater containing 100 ppm Cr, the process of Cr reduction took place and the Cr in the medium were reduced 92.7% within 20 days. The similar results were achieved on the treatment of PT VICO’s wastewater. The Cr content in the wastewater decreased from 6.54-6.76 to 0.08-0.09 ppm and the pH increased from 3 to 5 – 6 within 20 days incubation period.<br />Keywords: Cr detoxification, Desulfotomaculum orientis ICBB 1204, sulphate reducing bacteria, wastewater</p>

scholarly journals Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases

Biomolecules ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 752 ◽  
Author(s):  
Ivan Kushkevych ◽  
Věra Kotrsová ◽  
Dani Dordević ◽  
Leona Buňková ◽  
Monika Vítězová ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Growth Parameters ◽  
Sulfate Reducing Bacteria ◽  
Lactobacillus Species ◽  
Pcr Analysis ◽  
Isolation And Identification ◽  
Sulfate Reducing ◽  
Reducing Bacteria

The gut microbiota is a complex component of humans that depends on diet, host genome, and lifestyle. The background: The study purpose is to find relations between nutrition, intestinal lactic acid bacteria (LAB) from various environments (human, animal intestine, and yogurt) and sulfate-reducing microbial communities in the large intestine; to compare kinetic growth parameters of LAB; and to determine their sensitivity to different concentration of hydrogen sulfide produced by intestinal sulfate-reducing bacteria. Methods: Microbiological (isolation and identification), biochemical (electrophoresis), molecular biology methods (DNA isolation and PCR analysis), and statistical processing (average and standard error calculations) of the results were used. The results: The toxicity of hydrogen sulfide produced by sulfate-reducing bacteria, the survival of lactic acid bacteria, and minimal inhibitory concentrations (MIC) were determined. The measured hydrogen sulfide sensitivity values were the same for L. paracasei and L. reuteri (MIC > 1.1 mM). In addition, L. plantarum and L. fermentum showed also a similar sensitivity (MIC > 0.45 mM) but significantly (p < 0.05) lower than L. reuteri and L. paracasei (1.1 > 0.45 mM). L. paracasei and L. reuteri are more sensitive to hydrogen sulfide than L. fermentum and L. plantarum. L. pentosus was sensitive to the extremely low concentration of H2S (MIC > 0.15 mM). Conclusions: The Lactobacillus species were significantly sensitive to hydrogen sulfide, which is a final metabolite of intestinal sulfate-reducing bacteria. The results are definitely helpful for a better understanding of complicated interaction among intestinal microbiota and nutrition.

Biostimulation of sulfate-reducing bacteria and metallic ions removal from coal mine-impacted water (MIW) using shrimp shell as treatment agent

2020 ◽  
Vol 398 ◽  
pp. 122893 ◽  
Author(s):  
Caroline Rodrigues ◽  
Dámaris Núñez-Gómez ◽  
Hioná V. Dal Magro Follmann ◽  
Daniele D. Silveira ◽  
Maria Eliza Nagel-Hassemer ◽  
...  
Keyword(s):  
Coal Mine ◽  
Sulfate Reducing Bacteria ◽  
Metallic Ions ◽  
Sulfate Reducing ◽  
Shrimp Shell ◽  
Treatment Agent ◽  
Mine Impacted Water ◽  
Reducing Bacteria

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI PEREDUKSI SULFAT PADA AREA PERTAMBANGAN BATU BARA MUARA ENIM, SUMATERA SELATAN

2009 ◽  
Vol 10 (1) ◽  
pp. 26-35
Author(s):  
Muchamad Yusron ◽  
Bibiana W Lay ◽  
Anas M Fauzi ◽  
Dwi Andreas Santosa
Keyword(s):  
Biochemical Characterization ◽  
Mine Drainage ◽  
Sulfate Reducing Bacteria ◽  
Suitable Habitat ◽  
Isolation And Identification ◽  
Terminal Electron Acceptor ◽  
Isolation And Purification ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Physiological And Biochemical

Sulfate reducing bacteria utilize sulfate as their terminal electron acceptor and reduce it to sulphide. Acid mine drainage, by-products of mining activities, is an acidic sulfate-rich wastewater suitable habitat for sulfate reducing bacteria. Isolation and identification of sulfate reducing bacteria collected from Muara Enim coal mining, South Sumatra was carried out at Laboratory of Environmental Biotechnology, Indonesian Center for Biodiversity and Biotechnology (ICBB), Bogor, and Laboratory of Microbiology, Faculty of Veterinary, Bogor Agricultural University. Postgate B liquid media was used for isolation and purification via serial dilution. Physiological and biochemical characterization was done based on Bergeys Manual of Determinative Bacteriology. Fifteen pure isolates have been isolated with diverse characteristics. Eight isolates can sustain at pH 3, while the rest sustain at pH 4 or above. Sulfate reduction efficiency of each isolates were different, but increased as the pH increased. The bacteria are classified as Desulfovibrio sp., which is characterized straight rods, motile, non spore-forming and able to grow in simple organic carbon.

Specificity of Lectins Labeled with Colloidal Gold to the Exopolymeric Matrix Carbohydrates of the Sulfate-Reducing Bacteria Biofilm Formed on Steel

2020 ◽  
Vol 82 (5) ◽  
pp. 11-20
Author(s):  
D.R. Abdulina ◽  
◽  
L.M. Purish ◽  
G.O. Iutynska ◽  
◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Colloidal Gold ◽  
Steel Surface ◽  
Lectin Binding ◽  
Sulfate Reducing Bacteria ◽  
Gold Particles ◽  
Sulfate Reducing ◽  
Transmission Electron ◽  
Reducing Bacteria

The studies of the carbohydrate composition of the sulfate-reducing bacteria (SRB) biofilms formed on the steel surface, which are a factor of microbial corrosion, are significant. Since exopolymers synthesized by bacteria could activate corrosive processes. The aim of the study was to investigate the specificity of commercial lectins, labeled with colloidal gold to carbohydrates in the biofilm exopolymeric matrix produced by the corrosive-relevant SRB strains from man-caused ecotopes. Methods. Microbiological methods (obtaining of the SRB biofilms during cultivation in liquid Postgate B media under microaerophilic conditions), biochemical methods (lectin-binding analysis of 10 commercial lectins, labeled with colloidal gold), transmission electron microscopy using JEM-1400 JEOL. Results. It was shown using transmission electron microscopy that the binding of lectins with carbohydrates in the biofilm of the studied SRB strains occurred directly in the exopolymerіс matrix, as well as on the surfaces of bacterial cells, as seen by the presence of colloidal gold particles. For detection of the neutral carbohydrates (D-glucose and D-mannose) in the biofilm of almost all studied bacterial strains PSA lectin was the most specific. This lectin binding in biofilms of Desulfotomaculum sp. К1/3 and Desulfovibrio sp. 10 strains was higher in 90.8% and 94.4%, respectively, then for ConA lectin. The presence of fucose in the SRB biofilms was detected using LABA lectin, that showed specificity to the biofilm EPS of all the studied strains. LBA lectin was the most specific to N-аcetyl-D-galactosamine for determination of amino sugars in the biofilm. The amount of this lectin binding in D. vulgaris DSM644 biofilm was 30.3, 10.1 and 9.3 times higher than SBA, SNA and PNA lectins, respectively. STA, LVA and WGA lectins were used to detect the N-acetyl-Dglucosamine and sialic acid in the biofilm. WGA lectin showed specificity to N-acetyl-D-glucosamine in the biofilm of all the studied SRB; maximum number of bounded colloidal gold particles (175 particles/μm2) was found in the Desulfotomaculum sp. TC3 biofilm. STA lectin was interacted most actively with N-acetyl-D-glucosamine in Desulfotomaculum sp. TC3 and Desulfomicrobium sp. TC4 biofilms. The number of bounded colloidal gold particles was in 9.2 and 7.4 times higher, respectively, than using LVA lectin. The lowest binding of colloidal gold particles was observed for LVA lectin. Conclusions. It was identified the individual specificity of the 10 commercial lectins to the carbohydrates of biofilm matrix on the steel surface, produced by SRB. It was estimated that lectins with identical carbohydrates specificity had variation in binding to the biofilm carbohydrates of different SRB strains. Establishing of the lectin range selected for each culture lead to the reduction of the scope of studies and labor time in the researching of the peculiarities of exopolymeric matrix composition of biofilms formed by corrosiverelevant SRB.

The influence of potassium dichromate on dissimilatory reduction of sulfate and nitrate ions by bacteria Desulfovibrio sp.

2017 ◽  
Vol 28 (1-2) ◽  
pp. 84-95
Author(s):  
O. M. Moroz ◽  
S. O. Hnatush ◽  
Ch. I. Bohoslavets ◽  
T. M. Hrytsun’ ◽  
B. M. Borsukevych
Keyword(s):  
Electron Acceptor ◽  
Potassium Dichromate ◽  
Anaerobic Respiration ◽  
Sulfate Reducing Bacteria ◽  
Negative Influence ◽  
Electron Acceptors ◽  
Metal Compounds ◽  
Nitrate Ions ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Sulfate reducing bacteria, capable to reductive transformation of different nature pollutants, used in biotechnologies of purification of sewage, contaminated by carbon, sulfur, nitrogen and metal compounds. H2S formed by them sediment metals to form of insoluble sulfides. Number of metals can be used by these microorganisms as electron acceptors during anaerobic respiration. Because under the influence of metal compounds observed slowing of bacteria metabolism, selection isolated from technologically modified ecotops resistant to pollutions strains is important task to create a new biotechnologies of purification. That’s why the purpose of this work was to study the influence of potassium dichromate, present in medium, on reduction of sulfate and nitrate ions by sulfate reducing bacteria Desulfovibrio desulfuricans IMV K-6, Desulfovibrio sp. Yav-6 and Desulfovibrio sp. Yav-8, isolated from Yavorivske Lake, to estimate the efficiency of possible usage of these bacteria in technologies of complex purification of environment from dangerous pollutants. Bacteria were cultivated in modified Kravtsov-Sorokin medium without SO42- and FeCl2×4H2O for 10 days. To study the influence of K2Cr2O7 on usage by bacteria SO42- or NO3- cells were seeded to media with Na2SO4×10H2O or NaNO3 and K2Cr2O7 at concentrations of 1.74 mM for total content of electron acceptors in medium 3.47 mM (concentration of SO42- in medium of standard composition). Cells were also seeded to media with 3.47 mM Na2SO4×10H2O, NaNO3 or K2Cr2O7 to investigate their growth in media with SO42-, NO3- or Cr2O72- as sole electron acceptor (control). Biomass was determined by turbidymetric method, content of sulfate, nitrate, dichromate, chromium (III) ions, hydrogen sulfide or ammonia ions in cultural liquid – by spectrophotometric method. It was found that K2Cr2O7 inhibits growth (2.2 and 1.3 times) and level of reduction by bacteria sulfate or nitrate ions (4.2 and 3.0 times, respectively) at simultaneous addition into cultivation medium of 1.74 mM SO42- or NO3- and 1.74 mM Cr2O72-, compared with growth and level of reduction of sulfate or nitrate ions in medium only with SO42- or NO3- as sole electron acceptor. Revealed that during cultivation of bacteria in presence of equimolar amount of SO42- or NO3- and Cr2O72-, last used by bacteria faster, content of Cr3+ during whole period of bacteria cultivation exceeded content H2S or NH4+. K2Cr2O7 in medium has most negative influence on dissimilatory reduction by bacteria SO42- than NO3-, since level of nitrate ions reduction by cells in medium with NO3- and Cr2O72- was a half times higher than level of sulfate ions reduction by it in medium with SO42- and Cr2O72-. The ability of bacteria Desulfovibrio sp. to priority reduction of Cr2O72- and after their exhaustion − NO3- and SO42- in the processes of anaerobic respiration can be used in technologies of complex purification of environment from toxic compounds.

Removal of lead by sulfate-reducing bacteria in anaerobic continuous stirred tank reactors

2016 ◽  
Vol 14 (3) ◽  
pp. 557-561
Author(s):  
Nguyễn Thị Yên ◽  
Kiều Thị Quỳnh Hoa
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Stirred Tank ◽  
Synthetic Wastewater ◽  
Terminal Electron Acceptor ◽  
Stirred Tank Reactors ◽  
Sulfate Reducing ◽  
Sulfide Production ◽  
Continuous Stirred Tank ◽  
Continuous Stirred Tank Reactors ◽  
Reducing Bacteria

Lead contaminated wastewater negatively impacts to living organisms as well as humans. In recent years, a highly promising biological process using the anaerobic production of sulfide ions by sulfate-reducing bacteria has presented itself as an alternative option for the removal of lead. This process is based on microbial utilization of electron donors, such as organic compounds (carbon sources), and sulfate as the terminal electron acceptor for sulfide production. The biogenic hydrogen sulfide reacts with dissolved heavy metals to form insoluble metal sulfide precipitates Removal of lead by an enriched consortium of sulfate-reducing bacteria (DM10) was evaluated sulfate reduction, sulfide production and lead precipitation. Four parallel anaerobic continuous stirred tank reactors (CSTR, V = 2L) (referred as R1 - R4) were fed with synthetic wastewater containing Pb2+ in the concentrations of 0, 100, 150 and 200 mg L-1 of lead and operated with a hydraulic retention time of 5 days for 40 days. The loading rates of each metal in R1- R4 were 0, 20, 30 and 40 mg L-1 d-1, respectively. The results showed that there was no inhibition of SRB growth and that lead removal efficiencies of 99-100% for Pb2+ were achieved in R2 (100 mg L-1) and R3 (150 mg L-1) throughout the experiment. For the highest lead concentration of  200 mg L-1, a decrease in efficiency of removal (from 100 to 96%) was observed at the end of the experiment. The obtained result of this study might help for a better control operation and performance improvements of reactors.

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