Leukocyte activation and leukocyte procoagulant activities after blood contact with polystyrene and polyethylene glycol–immobilized polystyrene beads

Abstract The immunoreactivity of anti-triiodothyronine (anti-T3) IgG, pretreated at acidic pH and adsorbed onto polystyrene beads, was significantly greater than that determined for native anti-T3 IgG immobilized at optimum pH 3.5. Acidic-pH-pretreated IgG, adsorbed at pH 7.0 onto ethanol-treated beads, had less immunoreactivity than that on untreated beads and gave values similar to those of native IgG adsorbed onto activated beads at pH 9.6. The rate of immobilization onto treated beads was significantly greater than onto untreated ones, and the binding was reproducible (intra- and inter-batch coating CV, 3.7-4.6% and 7.2%, respectively) and very resistant to successive washings. A post-washing incubation in 10 mg/L bovine serum albumin solution was required to eliminate the decreased immobilized immunoreactivity caused by some washing reagents. For solid-phase radioimmunoassays of T3, 40% less antibody was needed when acidic-pH-pretreated IgG was adsorbed onto untreated beads or when native IgG was adsorbed onto ethanol-treated beads, in comparison with native IgG adsorbed onto untreated beads. Results and assay characteristics with such beads were comparable with results for solid-phase and liquid-phase polyethylene glycol assay of T3.

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Complement inhibition reduces material-induced leukocyte activation with PEG modified polystyrene beads (Tentagel™) but not polystyrene beads

Journal of Biomedical Materials Research Part A ◽

10.1002/jbm.a.30354 ◽

2005 ◽

Vol 74A (4) ◽

pp. 511-522 ◽

Cited By ~ 15

Author(s):

M. B. Gorbet ◽

M. V. Sefton

Keyword(s):

Leukocyte Activation ◽

Complement Inhibition ◽

Polystyrene Beads ◽

Modified Polystyrene

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Immunocytochemical detection of hepatic carbohydrate metabolic enzymes: Improved resolution with polyethylene glycol embedding and visio-bond semithin sections

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100124367 ◽

1992 ◽

Vol 50 (1) ◽

pp. 792-793

Author(s):

Kuixiong Gao ◽

Randal E. Morris ◽

Bruce F. Giffin ◽

Robert R. Cardell

Keyword(s):

Polyethylene Glycol ◽

Glycogen Phosphorylase ◽

Phosphoenolpyruvate Carboxykinase ◽

Glycogen Synthase ◽

Metabolic Enzymes ◽

Silver Stain ◽

Accurate Localization ◽

Immunocytochemical Detection ◽

Semithin Sections ◽

Parenchymal Cells

Several enzymes are involved in the regulation of anabolic and catabolic pathways of carbohydrate metabolism in liver parenchymal cells. The lobular distribution of glycogen synthase (GS), phosphoenolpyruvate carboxykinase (PEPCK) and glycogen phosphorylase (GP) was studied by immunocytochemistry using cryosections of normal fed and fasted rat liver. Since sections of tissue embedded in polyethylene glycol (PEG) show good morphological preservation and increased detectability for immunocytochemical localization of antigenic sites, and semithin sections of Visio-Bond (VB) embedded tissue provide higher resolution of cellular structure, we applied these techniques and immunogold-silver stain (IGSS) for a more accurate localization of hepatic carbohydrate metabolic enzymes.

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Directional observation on lipid of male nectary of Lindera megaphylla hemsl. by the rapid embedding method with polyethylene glycol (PEG)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100161151 ◽

1990 ◽

Vol 48 (3) ◽

pp. 718-719

Author(s):

Dai Dalin ◽

Guo Jianmin

Keyword(s):

Electron Microscope ◽

Polyethylene Glycol ◽

Chemical Reaction ◽

Traditional Method ◽

Osmium Tetroxide ◽

Unsaturated Lipid ◽

Embedding Method ◽

Long Period ◽

New Methods

Lipid cytochemistry has not yet advanced far at the EM level. A major problem has been the loss of lipid during dehydration and embedding. Although the adoption of glutaraldehyde and osmium tetroxide accelerate the chemical reaction of lipid and osmium tetroxide can react on the double bouds of unsaturated lipid to from the osmium black, osmium tetroxide can be reduced in saturated lipid and subsequently some of unsaturated lipid are lost during dehydration. In order to reduce the loss of lipid by traditional method, some researchers adopted a few new methods, such as the change of embedding procedure and the adoption of new embedding media, to solve the problem. In a sense, these new methods are effective. They, however, usually require a long period of preparation. In this paper, we do research on the fiora nectary strucure of lauraceae by the rapid-embedding method wwith PEG under electron microscope and attempt to find a better method to solve the problem mentioned above.

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Imaging sub-micron objects with light microscopy: Visualization of the T-4 bacteriophage

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100156158 ◽

1989 ◽

Vol 47 ◽

pp. 834-835

Author(s):

Malcolm Brown ◽

Reynolds M. Delgado ◽

Michael J. Fink

Keyword(s):

Electron Microscopy ◽

Light Microscopy ◽

Focal Plane ◽

Phase Contrast ◽

Dark Field ◽

E Coli ◽

Polystyrene Beads ◽

Television Camera ◽

Transmission Electron ◽

Universal Microscope

While light microscopy has been used to image sub-micron objects, numerous problems with diffraction-limitations often preclude extraction of useful information. Using conventional dark-field and phase contrast light microscopy coupled with image processing, we have studied the following objects: (a) polystyrene beads (88nm, 264nm, and 557mn); (b) frustules of the diatom, Pleurosigma angulatum, and the T-4 bacteriophage attached to its host, E. coli or free in the medium. Equivalent images of the same areas of polystyrene beads and T-4 bacteriophages were produced using transmission electron microscopy.For light microscopy, we used a Zeiss universal microscope. For phase contrast observations a 100X Neofluar objective (N.A.=1.3) was applied. With dark-field, a 100X planachromat objective (N.A.=1.25) in combination with an ultra-condenser (N.A.=1.25) was employed. An intermediate magnifier (Optivar) was available to conveniently give magnification settings of 1.25, 1.6, and 2.0. The image was projected onto the back focal plane of a film or television camera with a Carl Zeiss Jena 18X Compens ocular.

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