Relationships between the activity of respiratory-chain complexes in pre- (biopsy) or post-slaughter muscle samples and feed efficiency in random-bred Ghezel lambs

2017 ◽  
Vol 57 (8) ◽  
pp. 1674
Author(s):  
M. J. Zamiri ◽  
R. Mehrabi ◽  
G. R. Kavoosi ◽  
H. Rajaei Sharifabadi
Keyword(s):  
Respiratory Chain ◽  
Feed Intake ◽  
Enzyme Activities ◽  
Feed Efficiency ◽  
Average Daily Gain ◽  
Chain Complex ◽  
Respiratory Chain Complex ◽  
Respiratory Chain Complexes ◽  
Chain Complexes ◽  
Daily Gain

The present study was conducted to determine the relationship between the activity of mitochondrial respiratory chain complexes in pre- and post-slaughter muscle samples and residual feed intake (RFI) in Ghezel male lambs born as a result of random mating. The study was based on the hypothesis that random-bred lambs with lower feed (or higher) RFI have lower (or higher) respiratory chain-complex activity in muscle samples. Lambs (n = 30) were fed a diet consisting of 70% concentrate and 30% alfalfa hay during a 70-day period. Individual feed intake and average daily gain were recorded to calculate the RFI, feed-conversion ratio (FCR) and adjusted FCR (aFCR). On the basis of these calculations, the lambs were classified into low and high groups for RFI, with FCR and aFCR (n = 22) being one standard deviation above or below the means; this was corroborated by Student’s t-test (P < 0.01). At the end of the experiment, a 10-g biopsy sample was taken from the posterior side of the left femoral biceps. After 24 h, the lambs were slaughtered, and a sample from the posterior side of the right femoral biceps was dissected for determination of mitochondrial protein and respiratory chain-complex activities (Complexes I–V). The RFI was not correlated with the metabolic bodyweight and average daily gain, but was positively correlated (r = 0.56) with the average daily feed intake (P < 0.01); mean daily feed intake in the low-RFI group was 200 g less than that in the high-RFI group. The FCR and aFCR were not significantly (P > 0.05) correlated with average daily feed intake (r = 0.39 and r = 0.36 respectively), but showed a negative correlation (P < 0.01) with average daily gain (r = –0.73 and r = –0.76 respectively). Although very high negative correlations were recorded between the activities of all five respiratory-chain complexes and RFI in muscle samples obtained before (–0.91 to –0.97) and after (–0.92 to –0.97) slaughter, Complexes I and V showed small negative correlations (–0.40) with FCR or aFCR (P < 0.05). Enzyme activities of the respiratory-chain Complexes I, III and V were not significantly different between the pre- and post-slaughter biopsy samples; however, the enzyme activities of respiratory-chain Complexes II and IV were slightly higher in post-slaughter samples (P < 0.01). These results suggested that it may be possible to use the enzymatic activity of respiratory-chain complexes in muscle biopsy samples for screening of lambs for RFI, providing a useful procedure for genetic selection of lambs for this component of feed efficiency. These encouraging results need to be verified in further experiments using other sheep breeds and a larger number of lambs.

scholarly journals AML cells have low spare reserve capacity in their respiratory chain that renders them susceptible to oxidative metabolic stress

Blood ◽  
2015 ◽  
Vol 125 (13) ◽  
pp. 2120-2130 ◽  
Author(s):  
Shrivani Sriskanthadevan ◽  
Danny V. Jeyaraju ◽  
Timothy E. Chung ◽  
Swayam Prabha ◽  
Wei Xu ◽  
...  
Keyword(s):  
Respiratory Chain ◽  
Metabolic Stress ◽  
Chain Complex ◽  
Reserve Capacity ◽  
Respiratory Chain Complex ◽  
Respiratory Chain Complexes ◽  
Mitochondrial Mass ◽  
Chain Complexes ◽  
Key Points ◽  
Complex Activities

Key Points AML cells have increased mitochondrial mass, low respiratory chain complex activities, and low spare reserve capacity compared with normal cells. AML cells have heightened sensitivity to inhibitors of the respiratory chain complexes and oxidative stressors.

scholarly journals Effects of Xingpi Kaiyu Fang on ATP, Na/K-ATPase, and Respiratory Chain Complexes of Hippocampus and Gastrocnemius Muscle in Depressed Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Qingjie Yuan ◽  
Yang Li ◽  
Xiaofei Deng ◽  
Huawei Shi ◽  
Zhenwu Zhao ◽  
...  
Keyword(s):  
Atpase Activity ◽  
Respiratory Chain ◽  
Gastrocnemius Muscle ◽  
Chain Complex ◽  
Respiratory Chain Complex ◽  
Atp Content ◽  
Mitochondrial Ultrastructure ◽  
Respiratory Chain Complexes ◽  
Chain Complexes ◽  
Ultrastructural Damage

Objective. To clarify the effectiveness and mechanism of the Chinese herbal formula Xingpi Kaiyu Fang (XPKYF) which is composed of American ginseng (Xi-Yang-shen), Radix curcumae (Yu-Jin), Acori tatarinowii rhizoma (Shi-Chang-pu), and Hypericum perforatum (Guan-Ye-lian-qiao) in depressed rats. Methods. The rat model of depression was established by chronic unpredictable mild stress (CUMS) method for 6 weeks. Rats were randomly divided into six groups: control group, CUMS group, CUMS+XPKYF (3.6g/kg/d, 7.2g/kg/d, 14.4g/kg/d) groups, and CUMS+sertraline (4.5mg/kg/d) group. The sucrose preference test and the forced swimming test were performed to assess the rats’ depression behavior. Mitochondrial ultrastructure was observed by transmission electron microscope and adenosine triphosphate (ATP) content, sodium potassium ATPase (Na/K-ATPase) activity, and mitochondrial respiratory chain complexes activities in hippocampus and gastrocnemius muscle were measured at the 14th and 42nd day. Results. Rats subjected to six weeks of CUMS exhibited decreased sucrose preference ratio and prolonged immobility time. CUMS reduced ATP content in hippocampus, decreased Na/K-ATPase activity and respiratory chain complex I, III, and IV activities in hippocampus and gastrocnemius muscle, and damaged mitochondrial ultrastructure of hippocampus and gastrocnemius muscle. XPKYF at 14.4g/kg, the efficacy trend of which was better than the other drug groups, could prevent the stress-induced depressed behavior changes, inhibit the decrease of Na/K-ATPase activity in hippocampus, inhibit the decrease of respiratory chain complex III activities in hippocampus and gastrocnemius muscle, and protect mitochondria from ultrastructural damage. Conclusions. Energy deficiency and damaged mitochondrial ultrastructure were found in hippocampus and gastrocnemius muscle of depressed rats established by CUMS. XPKYF could partly reverse alterations in ATP, Na/K-ATPase, and respiratory chain complexes of hippocampus and gastrocnemius muscle and protect mitochondria from ultrastructural damage. This provides another experimental evidence for the clinical application of XPKYF in the treatment of depression.

scholarly journals 268 Administering an appeasing substance to optimize welfare and performance of receiving cattle

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 193-193
Author(s):  
Eduardo Colombo ◽  
Reinaldo F Cooke ◽  
Alice Brandão ◽  
Jacob Wiegand ◽  
Kelsey Schubach ◽  
...  
Keyword(s):  
Feed Intake ◽  
Feed Efficiency ◽  
Treatment Effects ◽  
Average Daily Gain ◽  
Plasma Concentrations ◽  
Feedlot Cattle ◽  
Skin Area ◽  
And Performance ◽  
Antimicrobial Treatments ◽  
Daily Gain

Abstract This experiment evaluated the impacts of bovine appeasing substance (BAS) administration on performance, health, and physiological responses of feedlot cattle during a 45-d receiving period. A total of 342 recently-weaned Angus-influenced steers, originating from 16 cow-calf operations, were obtained from an auction yard on d -1 and road-transported (12 h) to the feedlot. Upon arrival on d 0, body weight (BW) was recorded and steers were ranked by BW and source and assigned to receive BAS (Nutricorp, Araras, SP, Brazil; n = 171) or placebo (diethylene glycol monoethyl ether; CON; n = 171). Treatments (5 mL) were topically applied to the nuchal skin area of each animal. Within treatment, calves were allocated to 1 of 24 drylot pens (12 pens/treatment) and received a free choice total-mixed ration from d 1 to 45. Calves were assessed for bovine respiratory disease (BRD) signs and feed intake was recorded from each pen daily. Steer BW was again recorded on d 1, 7, 17, 31, and 45, whereas blood samples were collected from 5 steers/pen concurrently with each BW assessment. Average daily gain was greater (P = 0.05) in BAS vs. CON calves, although final BW did not differ (P = 0.36) between treatments. No treatment effects were detected for feed intake (P = 0.95), resulting in greater (P = 0.05) feed efficiency in BAS vs. CON steers. No treatment effects were detected (P ≥ 0.37) for plasma concentrations of haptoglobin, whereas plasma cortisol concentrations were greater (P = 0.05) in CON vs. BAS steers on d 7 (treatment × day; P = 0.07). Incidence of BRD was greater (P ≤ 0.05) in BAS vs. CON on d 6 to 10 and d 18 to 21 (treatment × day; P < 0.01), although overall BRD incidence did not differ (P = 0.24) between treatments. The number of antimicrobial treatments required per steer diagnosed with BRD symptoms to recover from sickness was greater (P = 0.04) in CON vs. BAS calves. No treatment differences were detected (P ≥ 0.41) for mortality incidence, or proportion of steers removed from the experiment due to extreme sickness. Results from this experiment indicate BAS administration upon feedlot entry improved average daily gain by enhancing feed efficiency. Administration of BAS facilitated earlier detection of BRD and reduced the need for antimicrobial treatments. Collectively, these results suggest BAS administration as a promising strategy to benefit performance and immunocompetence of feedlot receiving cattle.

PSIV-2 A comparison of feedlot growth and performance of beef x Holstein crossbred steers and Holstein steers

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 298-299
Author(s):  
Bailey L Basiel ◽  
Chad D Dechow ◽  
Tara L Felix
Keyword(s):  
Feed Intake ◽  
Feed Efficiency ◽  
Average Daily Gain ◽  
Corn Silage ◽  
Department Of Agriculture ◽  
Feedlot Performance ◽  
Performance Variables ◽  
Dried Distillers Grains ◽  
And Performance ◽  
Daily Gain

Abstract Objectives were to compare feedlot performance and carcass traits of F1 beef × Holstein steers and Holstein steers. Angus or Limousin × Holstein crossbred [n = 27; age = 12 ± 3 months; body weight (BW) = 435 ± 8 kg] and Holstein (n = 20; age = 11 ± 2 months; BW = 400 ± 9 kg) steers were fed at the Pennsylvania Department of Agriculture Livestock Evaluation Center for 111 days. Feed intake was recorded using the GrowSafe Feed Intake Monitoring System (Model 4000E, GrowSafe Systems Ltd., Calgary AB, Canada). The diet contained corn silage, dried distillers grains, soybean meal, and cracked corn and was formulated to meet or exceed the requirements of beef cattle (NASEM, 2016). Growth performance variables of interest and carcass measurements were analyzed with the Mixed procedure of SAS (SAS 9.4; SAS Institute Inc., Cary, NC). USDA assigned quality grades (QG) and yield grades (YG) were analyzed the with GLIMMIX procedure of SAS. Breed was a fixed effect in all models. There were no differences (P > 0.05) between breeds in average daily gain or feed efficiency. Crossbreds exceeded Holsteins in initial (P < 0.01) and final BW (P = 0.01), dry matter intake (P = 0.03), hot carcass weight (P < 0.01), backfat (P = 0.03), and ribeye area (REA; P < 0.01). Thirty-five percent (35%) of the Holsteins received a QG of Choice or above while 74% of crossbreds graded USDA Choice or above (P = 0.01). However, 75% of Holsteins were YG 2 or lower while only 45% of crossbreds achieved YG 2 or less (P = 0.05). There was no improvement in efficiency when crossbreds were compared to Holsteins; however, carcasses from crossbreds were more likely to grade USDA Choice or above while yielding greater REA and backfat than Holsteins.

Feeding frequency and its effect on feedlot performance in steers

1995 ◽  
Vol 75 (2) ◽  
pp. 255-257 ◽  
Author(s):  
L. A. Goonewardene ◽  
D. F. Engstrom ◽  
D. R. ZoBell
Keyword(s):  
Feed Intake ◽  
Feed Efficiency ◽  
Average Daily Gain ◽  
Mixed Diet ◽  
Feeding Frequency ◽  
Feedlot Performance ◽  
Or Efficiency ◽  
Beneficial Effects ◽  
Feedlot Steers ◽  
Daily Gain

One hundred and twenty commercial feedlot steers were penned and randomly allocated to three treatments: fed once, twice and three times per day, over a grower through finisher period of 196 d. Feeding frequency had no effect (P > 0.05) on average daily gain, feed intake or efficiency overall. We conclude that there are no beneficial effects in feeding a total mixed diet more frequently to feedlot steers. Key words: Feeding, frequency, gain, steers, feed efficiency

scholarly journals Glutathione and respiratory chain complex activity in duodenal mitochondria of broilers with low and high feed efficiency

2005 ◽  
Vol 84 (5) ◽  
pp. 782-788 ◽  
Author(s):  
C. Ojano-Dirain ◽  
M. Iqbal ◽  
T. Wing ◽  
M. Cooper ◽  
W. Bottje
Keyword(s):  
Respiratory Chain ◽  
Feed Efficiency ◽  
Chain Complex ◽  
Respiratory Chain Complex ◽  
Complex Activity ◽  
High Feed

scholarly journals Effect of inhibitors of mitochondrial respiratory chain complexes on the electromechanical activity in human myocardium

Medicina ◽  
2010 ◽  
Vol 46 (10) ◽  
pp. 679
Author(s):  
Vida Gendvilienė ◽  
Irma Martišienė ◽  
Danguolė Zablockaitė ◽  
Jonas Jurevičius
Keyword(s):  
Respiratory Chain ◽  
Control Level ◽  
Chain Complex ◽  
Mitochondrial Respiratory Chain ◽  
Human Myocardium ◽  
Contraction Force ◽  
Respiratory Chain Complexes ◽  
Complex Iv ◽  
Resting Tension ◽  
Chain Complexes

The aim of the study was to investigate the effect of inhibitors of mitochondrial respiratory chain complexes I, III, and IV on the electromechanical activity in human myocardium. Material and methods. The experiments were performed on the human myocardial strips obtained from patients with heart failure (NYHA class III or IV) using a conventional method of registration of myocardial electromechanical activity. Under the perfusion with physiological Tyrode solution (control), contraction force (P) was 0.94±0.12 mN (n=16), relaxation time (t50) was 173.38±5.03 ms (n=15), action potential durations measured at 50% (AP50) and 90% (AP90) repolarization were 248.96±13.38 ms and 398.59±17.93 ms, respectively (n=13). Results. The inhibition of respiratory chain complex I by rotenone (3×10–5 M, the highest concentration applied) decreased contraction force of human myocardium to 48.99%±14.74% (n=3) (P<0.05); AP50, to 81.34%±15.81%; and AP90, to 87.28%±7.25% (n=3) (P>0.05) of control level, while relaxation time and resting tension remained almost unchanged. Antimycin A, an inhibitor of complex III, applied at the highest concentration (3×10–4 M) reduced P to 41.66%±8.8% (n=5) (P<0.001) and marginally increased t50 and decreased the durations of AP. Anoxia (3 mM Na2S2O4) that inhibits the activity of complex IV reduced the contraction force to 9.23%±3.56% (n=6) (P<0.001), AP50 and AP90 to 65.46%±9.95% and 71.07%±8.39% (n=5) (P<0.05) of control level, respectively; furthermore, the resting tension augmented (contracture developed). Conclusions. Our results show that the inhibition of respiratory chain complex IV had the strongest inhibitory effect on the electromechanical activity of failing human myocardium.

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