Effect of Flemingia macrophylla silage on in vitro fermentation characteristics and reduced methane production

2020 ◽  
Vol 60 (16) ◽  
pp. 1918 ◽  
Author(s):  
Bounnaxay Viennasay ◽  
Metha Wanapat ◽  
Pajaree Totakul ◽  
Burarat Phesatcha ◽  
Thiwakorn Ampapon ◽  
...  
Keyword(s):  
Methane Production ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Flemingia Macrophylla ◽  
In Vitro Gas Production ◽  
Feeding Trials ◽  
Dietary Treatments ◽  
Kinetics Of

Fodder shrub, Flemingia macrophylla silage (FMS), was nutritionally evaluated for rumen fermentation characteristics using an in vitro gas production technique. Dietary treatments were imposed in a 2 × 7 factorial arrangement in a completely randomised design. The first factor was two ratios of roughage:concentrate (R:C at 60:40 and 40:60), and the second factor was seven levels of FMS supplementation at 0, 20, 40, 60, 80, 100 and 120 g/kg of dietary substrate. The results revealed that a R:C ratio at 40:60 increased the kinetics of gas production (P < 0.001). Moreover, supplementation of FMS increased in vitro degradability and the concentration of propionic acid (P < 0.001), whereas acetic acid concentration, protozoal population and methane production were reduced (P < 0.001). In conclusion, supplementation of FMS at 120 g/kg of dietary substrate improved rumen fermentation and subsequently reduced methane production. However, in vivo feeding trials using FMS should be conducted to gain more information for further implementations.

Comparison of four in vitro gas production methods to study rumen fermentation kinetics of starch rich feeds

1997 ◽  
Vol 1997 ◽  
pp. 196-196
Author(s):  
S. Fakhri ◽  
A. R. Moss ◽  
D.I. Givens ◽  
E. Owen
Keyword(s):  
Rumen Fermentation ◽  
Gas Production ◽  
Production Methods ◽  
Fermentation Kinetics ◽  
In Vitro Gas Production ◽  
Production Techniques ◽  
Kinetics Of

Recently, the automatic in vitro gas production techniques (e.g. Cone. 1994; Theodorou et al., 1994) have been developed to study rumen fermentation kinetics. Many approaches have been taken. This work investigates the suitability of different methods for estimating the rumen fermentation of two starch rich feedstuffs.

scholarly journals Effect of Ginger Essential Oil on in Vitro Gas Production, Rumen Fermentation and Methane Production

2021 ◽  
pp. 509-515
Author(s):  
Erdinç ALTINÇEKİÇ ◽  
Önder CANBOLAT ◽  
Şeniz ÖZİŞ ALTINÇEKİÇ
Keyword(s):  
Essential Oil ◽  
Methane Production ◽  
Rumen Fermentation ◽  
Gas Production ◽  
In Vitro Gas Production

Relationship between in vitro gas production profiles of forages and in vivo rumen fermentation patterns in beef steers fed those forages

2002 ◽  
Vol 98 (1-2) ◽  
pp. 13-24 ◽  
Author(s):  
V.E Brown ◽  
C Rymer ◽  
R.E Agnew ◽  
D.I Givens
Keyword(s):  
Rumen Fermentation ◽  
Gas Production ◽  
Beef Steers ◽  
In Vitro Gas Production

Using krabok (Irvingia malayana) seed oil and Flemingia macrophylla leaf meal as a rumen enhancer in an in vitro gas production system

2017 ◽  
Vol 57 (2) ◽  
pp. 327 ◽  
Author(s):  
Sungchhang Kang ◽  
Metha Wanapat ◽  
Kampanat Phesatcha ◽  
Thitima Norrapoke ◽  
Suban Foiklang ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Propionic Acid ◽  
Methane Production ◽  
Production System ◽  
Seed Oil ◽  
Gas Production ◽  
Flemingia Macrophylla ◽  
Production Kinetics ◽  
In Vitro Gas Production

An in vitro gas production system was conducted to investigate the effect of krabok (Irvingia malayana) seed oil (KSO) and Flemingia (Flemingia macrophylla) leaf powder (FLM) supplementation on gas production kinetics, volatile fatty acid (VFA) and methane production with different ratios of rice straw to cassava chip (RS : CC). The treatments were arranged according to a 4 × 2 × 2 factorial arrangement in a completely randomised design using four different ratios of RS : CC (100 : 0; 60 : 40; 20 : 80; and 0 : 100), two levels of KSO supplement (0% and 2.5% of total dietary substrate) and two levels of FLM supplement (0% and 2.5% of total dietary substrate). The gas production kinetics were affected by RS : CC, KSO and FLM supplementation (P < 0.05). However, there was no interaction between RS : CC*FLM, FLM*KSO, or RS : CC*FLM*KSO; with the exception of RS : CC*KSO (P < 0.05). Cumulative gas at 96 h post incubation was increased with increasing RS : CC especially at 0 : 100. However, KSO supplementation suppressed gas production whereas FLM could enhance gas production from feed fraction (P < 0.05). Increasing RS : CC ratio resulted in increasing total VFA, propionic acid and butyric acid whereas acetic acid concentration was reduced; therefore, ratio of acetic acid : propionic acid was reduced. However, KSO supplementation depressed VFA production whereas the FLM supplement had no effect. The methane production was reduced with increasing level of RS : CC especially with supplementation of KSO. Based on this study, it is concluded that KSO addition could reduce methane production whereas FLM could enhance the gas production and fermentation end products; hence, the combined use is potentially beneficial. However, further research under in vivo conditions should be conducted.

scholarly journals Effects of Microalgae Species on In Vitro Rumen Fermentation Pattern and Methane Production

2020 ◽  
Vol 20 (1) ◽  
pp. 207-218 ◽  
Author(s):  
Ekin Sucu
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Corn Silage ◽  
Fermentation Pattern ◽  
Proximate And Mineral Composition

AbstractThis experiment was conducted to establish the effects of two types of microalgae [Chlorella vulgaris (AI), C. variabilis (AII) and their combination (AI+AII)] with two substrates (wheat and corn silages) on rumen fermentation, gas and methane production. To each substrate, one of 3 algae treatment was supplemented at 0% and 25% of the total incubated dry matter. A series of 5 measurement points (3, 6, 12, 24 and 48 h) were completed and the gas production was monitored. The proximate and mineral composition of microalgae and substrates were examined. At 48 h incubation rumen fermentation variables and CH4 production were also assessed. When compared with wheat silage, corn silage caused an increase in gas production (P<0.05). Ruminal gas production decreased in the algae groups when compared to the controls (0% algae, wheat and corn silages, P<0.05). Among algae, C. vulgaris had the strongest effect, decreasing gas production by 34%. Among algae, the total volatile fatty acids (VFA) and CH4 production were found to be lower in C. variabilis (P<0.001). Ammonia-N increased with the algae inclusion (P<0.05). But, the ruminal gas production, pH, acetate, the total VFA, CH4 and rumen fermentation efficiency were not affected by the substrate and algae interaction (P>0.05). The propionate was the highest (P<0.05) for corn silage when incubated with C. vulgaris. Ruminal butyrate was the lowest for the wheat silage when incubated with the mixture of algae (P<0.05). The NH3-N was the highest in corn silage when incubated with all algae types (P<0.05). Careful selection and combination of substrate and algae may positively manipulate rumen fermentation and may inhibit CH4 production. Further research is needed to validate these results in vivo.

scholarly journals Residue of propolis extract in bovine diets with increasing levels of protein on rumen fermentation

2020 ◽  
Vol 55 ◽  
Author(s):  
Roberto Junior Teixeira Nascimento ◽  
Rafael Monteiro Araújo Teixeira ◽  
Thierry Ribeiro Tomich ◽  
Luiz Gustavo Ribeiro Pereira ◽  
Tânia Dayana do Carmo ◽  
...  
Keyword(s):  
Volatile Fatty Acids ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Propolis Extract ◽  
In Vitro Gas Production ◽  
Dry Matter Degradation ◽  
Kinetics Of

Abstract: The objective of this work was to evaluate the effect of the residue from the extraction of propolis, added to bovine diets with increasing levels of protein, on ruminal fermentation in vitro. For this, the in vitro gas production technique was used. Incubation was carried out with inocula from three fistulated cows, in three periods. In each period, a cow received a daily dose of 100 g propolis residue. Four diets were evaluated: corn silage (control); and 25, 50, and 75% concentrate based on soybean meal. The following were determined: kinetics of rumen fermentation; dry matter degradation; production of gases, volatile fatty acids (acetate, propionate, and butyrate), methane, and ammonia nitrogen; and pH. The inclusion of 14.4, 15.1, and 9.5% propolis residue, respectively, to 25, 50, and 75% concentrate increased the production of gases from the degradation of fibrous carbohydrates, when compared with the control. The propolis residue reduces methane production and the acetate:propionate ratio at all tested concentrate inclusion levels.

The prediction of in vivo methane production and animal performance from the in vitro gas production technique

2003 ◽  
Vol 2003 ◽  
pp. 157-157
Author(s):  
A. Bortolozzo ◽  
D. K. Lovett ◽  
S. Lovell ◽  
L. Stack ◽  
F. P. O’Mara
Keyword(s):  
Chemical Composition ◽  
Methane Production ◽  
Gas Production ◽  
Animal Performance ◽  
Production Technique ◽  
In Vitro Gas Production ◽  
Ad Libitum

The in vivo determination of methane (CH4) production requires specialist equipment which is costly to maintain. Whilst the in vitro gas production technique has been demonstrated to show potential to rank diets for their methanongenic potential at maintenance planes of nutrition (Moss and Givens, 1997) no study has investigated this relationship when feedstuffs are fed ad libitum. The objective of this study was to assess the ability of the technique to predict in vivo CH4 production and animal performance from six diets differing in their chemical composition.

scholarly journals Simulation of rumen fermentation kinetics of by-products from the biodiesel industry with in vitro gas production technique

2015 ◽  
Vol 36 (6) ◽  
pp. 3851 ◽  
Author(s):  
Alex Lopes da Silva ◽  
Marcos Inácio Marcondes ◽  
Cristina Mattos Veloso ◽  
Fernanda Campos de Sousa ◽  
Leonardo Sidney Knupp
Keyword(s):  
Degradation Rate ◽  
Palm Kernel ◽  
Rumen Fermentation ◽  
Gas Production ◽  
In Vitro Gas Production ◽  
The Mean ◽  
Kinetics Of ◽  
By Products ◽  
Biodiesel Industry

The objective of this study was to investigate the rumen fermentation kinetics of 18 by-products from the biodiesel industry exhibiting potential for use in the feeding of ruminants via the in vitro gas production technique. The following feeds were investigated: cottonseed, canudo de pito, crambe, sunflower, castor seed (detoxified with lime) and soybean meals and cottonseed, peanut, babassu, crambe, palm kernel, sunflower, licuri nut, macaúba, forage radish and jatropha cakes. The evaluated parameters were total gas production (VfT), gas production from fibrous carbohydrates (VfFC), gas production from non-fibrous carbohydrates (VfNFC), the degradation rate of fibrous carbohydrates (kdFC), the degradation rate of non-fibrous carbohydrates (kdNFC) and lag time (lag). The feeds were grouped into six different groups according to rumen fermentation kinetic parameters and adopting an R2 of 0.8. Forage radish cake and the meals of cottonseed, soybean, crambe and sunflower composed the first group, while the cakes of babassu and sunflower formed the second group. Canudo de pito and castor seed meals and the cakes of cottonseed, licuri and jatropha I and II formed the third group. The fourth group was composed by the cakes of crambe, palm kernel and peanut I. The fifth group was formed by peanut cake II, while macauba fruit cake formed the sixth group. The VfNFC and VfFC varied from 16.72 to 200.07 mL and from 53.09 to 242.12 mL, respectively. The mean kdFC and kdNFC values varied from 0.002 to 0.039% h-1and from 0.022 to 0.430% h-1, respectively. The mean lag and VfT varied from 0.0001 to 5.2029 hours and 136.94 to 301.44 mL, respectively. A number of the products exhibited the potential to replace soybean meal, especially the forage radish cake and cottonseed, crambe and sunflower meals.

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