Relationship between in vitro gas production profiles of forages and in vivo rumen fermentation patterns in beef steers fed those forages

2002 ◽  
Vol 98 (1-2) ◽  
pp. 13-24 ◽  
Author(s):  
V.E Brown ◽  
C Rymer ◽  
R.E Agnew ◽  
D.I Givens
Keyword(s):  
Rumen Fermentation ◽  
Gas Production ◽  
Beef Steers ◽  
In Vitro Gas Production

Effect of Flemingia macrophylla silage on in vitro fermentation characteristics and reduced methane production

2020 ◽  
Vol 60 (16) ◽  
pp. 1918 ◽  
Author(s):  
Bounnaxay Viennasay ◽  
Metha Wanapat ◽  
Pajaree Totakul ◽  
Burarat Phesatcha ◽  
Thiwakorn Ampapon ◽  
...  
Keyword(s):  
Methane Production ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Flemingia Macrophylla ◽  
In Vitro Gas Production ◽  
Feeding Trials ◽  
Dietary Treatments ◽  
Kinetics Of

Fodder shrub, Flemingia macrophylla silage (FMS), was nutritionally evaluated for rumen fermentation characteristics using an in vitro gas production technique. Dietary treatments were imposed in a 2 × 7 factorial arrangement in a completely randomised design. The first factor was two ratios of roughage:concentrate (R:C at 60:40 and 40:60), and the second factor was seven levels of FMS supplementation at 0, 20, 40, 60, 80, 100 and 120 g/kg of dietary substrate. The results revealed that a R:C ratio at 40:60 increased the kinetics of gas production (P < 0.001). Moreover, supplementation of FMS increased in vitro degradability and the concentration of propionic acid (P < 0.001), whereas acetic acid concentration, protozoal population and methane production were reduced (P < 0.001). In conclusion, supplementation of FMS at 120 g/kg of dietary substrate improved rumen fermentation and subsequently reduced methane production. However, in vivo feeding trials using FMS should be conducted to gain more information for further implementations.

Comparison of four in vitro gas production methods to study rumen fermentation kinetics of starch rich feeds

1997 ◽  
Vol 1997 ◽  
pp. 196-196
Author(s):  
S. Fakhri ◽  
A. R. Moss ◽  
D.I. Givens ◽  
E. Owen
Keyword(s):  
Rumen Fermentation ◽  
Gas Production ◽  
Production Methods ◽  
Fermentation Kinetics ◽  
In Vitro Gas Production ◽  
Production Techniques ◽  
Kinetics Of

Recently, the automatic in vitro gas production techniques (e.g. Cone. 1994; Theodorou et al., 1994) have been developed to study rumen fermentation kinetics. Many approaches have been taken. This work investigates the suitability of different methods for estimating the rumen fermentation of two starch rich feedstuffs.

scholarly journals Effect of Eucalyptus globulus leaves extracts on in vitro rumen fermentation, methanogenesis, degradability and protozoa population

2018 ◽  
Vol 18 (3) ◽  
pp. 753-767 ◽  
Author(s):  
Amina Boussaada ◽  
Rabah Arhab ◽  
Serena Calabrò ◽  
Raffaella Grazioli ◽  
Maria Ferrara ◽  
...  
Keyword(s):  
Organic Matter ◽  
Ethyl Acetate ◽  
Eucalyptus Globulus ◽  
Ethyl Acetate Extract ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Aqueous Extracts ◽  
Ch4 Production ◽  
In Vitro Gas Production

Abstract The aim of the research was to evaluate the effect of three Eucalyptus globulus extracts rich in phenolic compounds, especially flavonoids, on rumen fermentation, methane (CH4) production, organic matter degradability and protozoa population using an in vitro gas production technique. Four concentrations (0, 50, 75 and 100 mg) of three Eucalyptus extracts (ethyl acetate, n-butanol and aqueous) were added to a diet of ruminants (forage: concentrate ratio 60:40) and incubated at 39°C under anaerobiosis with buffered rumen fluid. After 24 h, the fermentation fluid was analysed for ammonia-N and volatile fatty acids (VFA). Organic matter degradability (OMD) and protozoa were also determined; in vitro gas production was also recorded and CH4 concentration was measured. Compared to the control, CH4 production was significantly lower for ethyl acetate extract (P<0.05), but higher for n-butanol and aqueous extracts. Production of ammonia- N was lower in all Eucalyptus extracts (P<0.05). Propionate production (P<0.05) increased for ethyl acetate and n-butanol extracts, whereas no effect was registered for VFA, for all Eucalyptus extracts. Ethyl acetate extract decreased in vitro OMD (P<0.05), whereas n-butanol and aqueous extracts were comparable to the control. Protozoa population decreased (P<0.05) for all extracts in comparison with the control. Eucalyptus ethyl acetate extract might be promising to be used as a potent anti-methanogenic additive. Moreover, the assessment of the right dosage seems to be important to decrease methane production, without reducing feed nutritional value.

scholarly journals Rumen fermentation kinetics as impacted by red amaranth (Amaranthus cruentus, L) leaf powder using in vitro gas production technique

2020 ◽  
Author(s):  
Thiwakorn Ampapon ◽  
Bounnaxay Viennasay ◽  
Metha Wanapat
Keyword(s):  
Rumen Fermentation ◽  
Gas Production ◽  
Amaranthus Cruentus ◽  
Randomized Design ◽  
In Vitro Gas Production ◽  
Two Factors ◽  
Dry Matter Degradation ◽  
Leaf Powder ◽  
Completely Randomized Design

Abstract Background A need for research searching for alternative rumen enhancers warrants immediate attention. The in vitro fermentation experiment was conducted using factorial arrangement of two factors of roughage to concentrate and seven level of red amaranth leaf powder percentage of total substrate in a Completely randomized design (CRD). Two factors, namely Factor A was two ratio of roughage (R) to concentrate (C) at 60:40 and 40:60 and Factor B was level of red amaranth (Amaranthus cruentus, L) leaf powder (RALP) supplementation at 0, 2, 4, 6, 8, 10, and 12% of total dietary substrate. Results Red amaranth leaf powder (RALP) contained phytonutrients both condensed tannins and saponins in addition with high macro minerals (Ca, K, and Mg). This experiment revealed innovations of the RALP supplementation by enhancing rumen propionate (C3) production, reducing acetate (C2) to (C3) ratio, reducing protozoal population and mitigating methane (CH4) production. Furthermore, rumen dry matter degradation percentages were remarkably enhanced (P < 0.001) by increasing RALP supplementation. Conclusion Plants rich in phytonutrients and minerals such as red amaranth leaf powder (RALP) have a vital and promising role in modulating rumen fermentation, mitigating methane production, as well as increasing substrate degradability.

Fermentation profiles and degradability measurements in extrusa diet samples collected from brome-suppressed and undisturbed pastures and their relationship to weight gain of steers

2004 ◽  
Vol 84 (1) ◽  
pp. 105-111 ◽  
Author(s):  
M. Blümmel ◽  
E. E. Grings ◽  
M. R. Haferkamp
Keyword(s):  
Weight Gain ◽  
Gompertz Model ◽  
Gas Production ◽  
Beef Steers ◽  
N Level ◽  
Nutritive Quality ◽  
In Vitro Gas Production ◽  
Grazing Period ◽  
Gas Volume

The effects of suppression of annual bromes (Bromus japonicus Thunb. and Bromus tectorum L.) by atrazine application on the nutritive quality of extrusa diet samples (EDS) collected from the esophagus were investigated, and EDS quality estimates were compared with weight gain of grazing steers. Analysis on EDS included crude protein (CP), in vitro organic matter degradability (IVOMD), and gas production profiles in N supplemented and unsupplemented incubation media. Brome-suppression tended (P = 0.07) to increase CP content but effects on gas production kinetics and IVOMD were dependent on incubation medium N-level. In N-unsupplemented incubations, asymptotic gas production was less and rates of gas production were greater in EDS from brome-suppressed compared to undisturbed pasture. No such differences were found for N-supplemented incubations. Weight gains of steers grazing brome-suppressed pastures were 16% greater (P = 0.007) than from control pastures. The R2 for the comparison of predicted and measured gains were 0.90 (P < 0.0001), 0.96 (P < 0.0001), and 0.90 (P < 0.0001) using CP, IVOMD (N-low), and IVOMD (N-rich) as the predicting variable, respectively. Best predictions using in vitro gas production measurements were obtained from 24 h gas volume recording (R2 = 0.93, P < 0.0001). Best-fit model (sigmoidal vs. exponential) depended on grazing period and N-level, and the sigmoidal Gompertz model best described most gas production profiles. Key words: Forage quality, gas production, weight gain, beef steers

scholarly journals Determination of the In Vitro Gas Production and Potential Feed Value of Olive, Mulberry and Sour Orange Tree Leaves

2018 ◽  
Vol 13 (1) ◽  
pp. 269-278 ◽  
Author(s):  
Mustafa Olfaz ◽  
Unal Kilic ◽  
Mustafa Boga ◽  
Abdiwali Mohamoud Abdi
Keyword(s):  
Nutritive Value ◽  
Condensed Tannin ◽  
Gas Production ◽  
Net Energy ◽  
Sour Orange ◽  
Tree Leaves ◽  
Randomized Design ◽  
In Vitro Gas Production

AbstractThis study was conducted to determine the potential nutritive value andin vitrogas production (IVGP) parameters ofOlea europaea L. (Olive = OL),Morus alba L. (Mulberry = ML) andCitrus aurantium L. (Sour orange = SOL) tree leaves. Hohenheim gas test was used to determine thein vitrogas productions of the leaves. The gas production of samples over time was recorded for 3, 6, 9, 12, 24, 48, 72 and 96 h after incubation. Completely Randomized Design was used to compare gas production, and gas production kinetics of samples. The findings of the present study suggested that there were differences among the tree leaves in terms of crude protein, NDF,in vitrogas productions, organic matter digestibility (OMD), metabolisable energy (ME), net energy lactation (NEL) and relative feed values (RFV) (P<0.01). ML had the highest condensed tannin contents (P<0.05),in vitrogas production (IVGP), OMD and energy values (P<0.01). SOL had highest RFV values. OL showed the lowest IVGP when compared to SOL and ML. Low NDF and ADF contents of SOL would probably increase the voluntary intake, digestibility and relative feed values of these leaves by ruminants. In conclusion, it was determined that OL, ML and SOL used in the study have lowin vitrogas production and can be utilized as alternative roughage feed in ruminants. However, it is recommended that the results obtained from this research should be tested inin vivostudies.

Effect of type and inclusion level of cold-pressed oilseed cakes on in vitro rumen fermentation

2014 ◽  
Vol 54 (10) ◽  
pp. 1709 ◽  
Author(s):  
H. Benhissi ◽  
A. García-Rodríguez ◽  
I. Beltrán de Heredia
Keyword(s):  
Rumen Fermentation ◽  
Gas Production ◽  
Barley Straw ◽  
High Fat ◽  
In Vitro Gas Production ◽  
Fat Level ◽  
Total Fat ◽  
Cold Pressed ◽  
In Vitro Rumen Fermentation

The current study was conducted to explore the impact of inclusion of different types and levels of cold-pressed oilseed cakes on in vitro rumen fermentation and gas production parameters. Two batch fermentation trials (24 and 96 h) were conducted using the in vitro gas production technique. In each trial, three types of lipid sources [palm fat (PF), cold-pressed rapeseed cake (CPRC) and cold-pressed sunflower cake (CPSC)] × two levels of total fat (30 and 60 g/kg DM) were evaluated. Incubated substrates were made from iso-energetic and isoproteic basal mixtures of barley straw and concentrate (ratio 10 : 90). During the 24-h incubation trial, samples were collected 24 h post-incubation to measure volatile fatty acids (VFA) and methane production. The 96-h incubation was used to assess the rate and extent of in vitro gas production. CPSC showed lower total VFA (P < 0.001) and methane (P < 0.001) production, compared with PF and CPRC. High fat level decreased total VFA (P < 0.001) and methane (P < 0.001) production compared with low level of inclusion. Molar proportions of individual VFA and acetate to propionate ratio were not affected by lipid supplementation. CPSC reduced (P = 0.006) asymptotic gas production at high fat level, compared with PF and CPRC. Neither supplementation level nor type of lipid affected the fractional gas production rate or lag time. In conclusion, there was no difference in ruminal fermentation when either CPRC or PF were added but CPSC altered microbial fermentation in vitro at a total fat level of 60 g/kg DM.

scholarly journals Effect of Replacing Alfalfa Hay with Acacia Foliage on the Growth Performance, In Vitro Gas Production and Rumen Fermentation in Goats

2019 ◽  
Vol 7 (9) ◽  
Author(s):  
Ahmed M. El-Waziry ◽  
Saeid M. Basmaeil ◽  
Abdallah N. Al-Owaimer ◽  
Hassan M. Metwally ◽  
Muttaher H. Ali ◽  
...  
Keyword(s):  
Growth Performance ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Alfalfa Hay ◽  
In Vitro Gas Production
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