Body composition in vivo. VII. Relation between red cell volume and total body water in ewes

1965 ◽  
Vol 16 (4) ◽  
pp. 661 ◽  
Author(s):  
BA Panaretto ◽  
DA Little

The relationship between total body water and red cell volume was determined in a group of non-pregnant crossbred ewes, in an endeavour to obtain a method which did not necessitate the use of radioisotopes for determining body composition. Total body water could be predicted from red cell volume. The 95% confidence interval for a mean value for total body water, predicted from the red cell volume, was ±18.8% of the mean. All other relations which can be derived between the red cell volume and other body parameters depended on this relation. The calculation of body composition from measurements of the red cell volume is discussed.

1962 ◽  
Vol 8 (3) ◽  
pp. 283-289 ◽  
Author(s):  
R. D. Hyde ◽  
N. F. Jones

Blood ◽  
1962 ◽  
Vol 19 (1) ◽  
pp. 89-98 ◽  
Author(s):  
SERGIO PIOMELLI ◽  
DAVID G. NATHAN ◽  
JAMES F. CUMMINS ◽  
FRANK H. GARDNER ◽  
Alvera L. Limauro

Abstract 1. Studies of red cell volume, total body water and extracellular water have been carried out in a group of octogenarians and in a group of young men. 2. Differences in total red cell volume, blood volume and the distribution of body water were detected. 3. The implications and interpretations of these findings are discussed.


1963 ◽  
Vol 14 (6) ◽  
pp. 926 ◽  
Author(s):  
BA Panaretto ◽  
AR Till

The antipyrine, tritiated water, and N-acetyl-4-aminoantipyrine spaces were determined simultaneously in goats which had been deprived of feed and water for 48 hr. The animals were then killed, minced, and analysed for water, fat, protein, and ash contents. The compositions of the whole and empty bodies of the goats were calculated, and the relationships between the bodily components were compared with those reported for cattle, sheep, and some monogastric species. The relationships found between the components of the whole bodies compared favourably with those derived from the empty bodies. The relationships of the spaces determined in vivo to total body water, fat, and protein were found, and confidence statements were placed on predicted estimates.


1988 ◽  
Vol 59 (1) ◽  
pp. 109-124 ◽  
Author(s):  
R. J. Johnson ◽  
D. J. Farrell

1. Birds (n169) which varied in age, live weight, nutritional history, physiological state and genotype were slaughtered and analysed for total body water. Before slaughter, birds were injected with the water isotopes tritiated water (TOH) or deuterium oxide (D2O), or both, to determine TOH space or D2O space, or both, as estimates of total body water in vivo.2. At the mean total body water of all birds determined by desiccation, of 1096·4 (SD 424·1) g, TOH space and D2O space overestimated total body water by 10·4 and 8·5 % respectively. The difference between the isotopes was significant (P< 0·05).3. Based on recovery of isotope it was postulated that the main reason for the observed overestimation of total body water in vivo was incomplete recovery of isotope due to the vacuum sublimation technique. The mean recovery (%) of added isotope to whole blood after vacuum sublimation was 93·0 (SD 2·6) and 92·4 (SD 5·5) of the theoretical concentrations of TOH and D2O respectively.4. Nevertheless, accurate prediction of total body water was obtained from regression equations which included live weight and isotope-dilution space. Values required logarithmic (base 10) transformation before derivation of linear and multiple linear regression equations, and the precision of prediction was determined by the residual standard deviation (RSD).5. Total body water could be predicted with nearly equal accuracy from live weight or isotope-dilution space (RSD 0·025 and 0·020 respectively). Prediction of carcass protein was more accurate from live weight (RSD 0·033) than from TOH space (RSD 0·036), and inclusion of both variables resulted in only a marginal decrease in RSD to 0·031.6. The prediction of carcass fat and energy was markedly improved by the inclusion of isotope-dilution space in conjunction with live weight compared with live weight alone.7. The relations show the developmental nature of body composition of domestic fowl given diets adequate in nutrients. The prediction equations demonstrate the precision possible for studies in which estimates of body composition in poultry are required without slaughter.


2001 ◽  
Vol 2001 ◽  
pp. 206-206 ◽  
Author(s):  
R. E. Agnew ◽  
W J McCaughey ◽  
J.D. McEvoy ◽  
D C Patterson ◽  
M G Porter ◽  
...  

San Pietro and Rittenberg (1953) reported that urea appeared to meet all the requirements of a satisfactory tracer. Urea is non toxic, not foreign to the body and it shows an even and rapid distribution throughout the total body water without any physiological effect. For these reasons in addition to its easy and accurate measurement, urea is an ideal candidate tracer to estimate empty body water in vivo. Total body water volume (urea space) can be estimated by dividing the total amount of urea infused by the increase in plasma urea concentration from prior to infusion until 12 or 30 minutes after mean infusion time. Kock and Preston (1973) reported significant relationships between urea space measurements and percentage of empty body fat and water in cattle. However, Andrew et al. (1995) using 21 Holstein cows showed that prediction of empty body water using the urea space technique only explained 31 % of the variation. The objective of this experiment was to use the urea dilution technique to estimate the body composition of lactating dairy cows and produce relationships between urea space and body fat and protein content.


1968 ◽  
Vol 19 (2) ◽  
pp. 267 ◽  
Author(s):  
BA Panaretto

Correlations are described between tritiated water space, total body water, fat, and protein in sheep subjected to 18–21 hr of fasting. These provide a system for estimating the body composition of living ruminants.


1999 ◽  
Vol 1999 ◽  
pp. 50-50
Author(s):  
S. De Campeneere ◽  
L.O. Fiems ◽  
J.M. Vanacker ◽  
B.G. Cottyn ◽  
Ch.V. Boucqué

Urea is non-toxic, not foreign to the body and it shows an even and rapid distribution throughout the total body water without any physiological effect or toxic manifestation. For these reasons and for its easy and accurate measurement, urea is an ideal tracer to estimate body composition in vivo. Total body water volume (urea space) can be estimated by dividing the total amount of urea infused by the increase in plasma urea concentration between prior to infusion and 12, 18 or 24 min after mean infusion time (Preston and Kock, 1973). In this experiment the urea infusion technique was evaluated to estimate body composition of Belgian Blue double-muscled bulls.


1965 ◽  
Vol 45 (1) ◽  
pp. 14-21 ◽  
Author(s):  
T. D. D. Groves ◽  
A. J. Wood

The method of Keston et al. (J. Biol. Chem. 122, 227) for the in-vivo determination of total body water when applied to the growing piglet has been evaluated and found to produce values in good agreement with those obtained by desiccation of the same animals.The densitometric method for the determination of deuterium oxide provides results of sufficient precision when considered in relation to the other unavoidable errors involved in work with live animals. The relative simplicity of the techniques and equipment in the present investigation recommend them for more extensive use in sequential studies of the body composition of growing animals.


1993 ◽  
Vol 70 (2) ◽  
pp. 433-438 ◽  
Author(s):  
N. Battistini ◽  
F. Virgili ◽  
G. Bedogni ◽  
G. R. Gambella ◽  
A. Bini

Total body electrical conductivity (TOBEC) is a simple and non-invasive method for the assessment of body composition in vivo. Information regarding the applicability of TOBEC in the condition of abnormal fluid balance is scarce. In the present paper we give the results of the comparison between TOBEC and total body water (TBW; assessed by the tritium dilution technique) in three groups of animals: (1) healthy (n 17), (2) expanded fluid volume by secondary biliary cirrhosis (SBC; n 9) and (3) Fiirosemide®-treated rats (n 9). The TOBEC score and TBW by tritium dilution were found to be highly correlated in the pooled sample (r 0·90) and in normal (r 0.·87), SBC (r 0·73) and Furosemide-treated (r 0·89) rats. However, the relationship between TOBEC and TBW, described by least-squares regression analysis, was found to be similar for SBC and normal rats but was significantly different for Furosemide-treated and normal rats. These findings suggest that TOBEC is unable to track TBW accurately when the ratio between intracellular and extracellular water is chronically or acutely altered.


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