Acid Phosphatase Activity in the Digestive System of the Desert Locust Schistocerca Gregaria (Forskal)

Acid phosphatase activity was determined biochemically in a homogenate of the digestive tract of the desert locust, S. gregaria. p-Nitrophenol was used as colorimetric standard and disodium p.nitrophenyl phosphate as substrate.

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Electron-cytochemical localization of acid phosphatase activity in the digestive caeca of the desert locust

Journal of the Royal Microscopical Society ◽

10.1111/j.1365-2818.1968.tb00613.x ◽

1968 ◽

Vol 88 (2) ◽

pp. 279-289 ◽

Cited By ~ 27

Author(s):

I. D. BOWEN

Keyword(s):

Acid Phosphatase ◽

Phosphatase Activity ◽

Acid Phosphatase Activity ◽

Desert Locust ◽

Cytochemical Localization

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PHOSPHOMONOESTERASE ACTIVITY IN THE LIFE-CYCLE STAGES OF SCHISTOCERCA GREGARIA

The Canadian Entomologist ◽

10.4039/ent100649-6 ◽

1968 ◽

Vol 100 (6) ◽

pp. 649-655 ◽

Cited By ~ 3

Author(s):

S. N. H. Naqvi ◽

Shahid H. Ashrafi ◽

M. A. H. Qadri

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Embryonic Development ◽

Phosphatase Activity ◽

Alkaline Phosphatase Activity ◽

Acid Phosphatase Activity ◽

Schistocerca Gregaria ◽

Adult Males ◽

First Instar ◽

Almost All

AbstractThe acid and alkaline phosphatase activity was measured in the developing egg and in the alimentary canal of aging nymphs as well as adult males and females of different ages. Para-nitrophenol was used as colorimetric standard and disodium p-nitrophenyl phosphate as substrate. Activity was measured in terms of micromoles of p-nitrophenol liberated from the substrate as a result of enzyme action.Acid phosphatase activity was noticed to increase with the embryonic development and was higher than in the case of alkaline phosphatase. The alkaline phosphatase activity was lowest in the freshly laid egg, but increased more sharply than acid phosphatase during embryonic development.The activity of both the acid and alkaline phosphatases was highest in the first instar and declined gradually to the fifth instar. The activity of acid phosphatase was higher than alkaline phosphatase in all stages except the first instar where it was almost equal. The activity of both the enzymes was higher during the intermoulting period and declined at each moult indicating a hormone–enzyme relationship.In adults, activity of both the enzymes increased up to the maturation period after which the activity gradually decreased. Acid phosphatase activity was generally higher in males whereas alkaline phosphatase activity was generally higher in females. In almost all cases, the acid phosphatase activity was found to be higher than the alkaline phosphatase.

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Erythrophagocytosis in the Liver in Hemolytic Anemias

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100100664 ◽

1968 ◽

Vol 26 ◽

pp. 184-185

Author(s):

O. T. Minick ◽

E. Orfei ◽

F. Volini ◽

G. Kent

Keyword(s):

Acid Phosphatase ◽

Oxidative Damage ◽

Phosphatase Activity ◽

Kupffer Cells ◽

Acid Phosphatase Activity ◽

Common Type ◽

Red Cell ◽

Cell Fragments ◽

Reticulo Endothelial System ◽

Important Site

Hemolytic anemias were produced in rats by administering phenylhydrazine or anti-erythrocytic (rooster) serum, the latter having agglutinin and hemolysin titers exceeding 1:1000.Following administration of phenylhydrazine, the erythrocytes undergo oxidative damage and are removed from the circulation by the cells of the reticulo-endothelial system, predominantly by the spleen. With increasing dosage or if animals are splenectomized, the Kupffer cells become an important site of sequestration and are greatly hypertrophied. Whole red cells are the most common type engulfed; they are broken down in digestive vacuoles, as shown by the presence of acid phosphatase activity (Fig. 1). Heinz body material and membranes persist longer than native hemoglobin. With larger doses of phenylhydrazine, erythrocytes undergo intravascular fragmentation, and the particles phagocytized are now mainly red cell fragments of varying sizes (Fig. 2).

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