Molecular genetic diversity and population structure in Eucalyptus pauciflora subsp. pauciflora (Myrtaceae) on the island of Tasmania

2014 ◽  
Vol 62 (3) ◽  
pp. 175 ◽  
Author(s):  
Archana Gauli ◽  
Dorothy A. Steane ◽  
René E. Vaillancourt ◽  
Brad M. Potts
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Molecular Genetic ◽  
Glacial Refugia ◽  
Altitudinal Distribution ◽  
The North ◽  
Chloroplast Haplotype ◽  
Molecular Genetic Diversity ◽  
Nuclear Microsatellite ◽  
Chloroplast Dna Markers

Genetic diversity and population structure of Tasmanian populations of Eucalyptus pauciflora were assessed using chloroplast and nuclear microsatellite markers. Maternal trees and open-pollinated progeny from 37 populations were sampled across the species’ geographic and altitudinal distribution in Tasmania. The distribution of chloroplast haplotype richness showed a clear geographic structure with suggestion of three major refugia (Storm Bay, Tamar Valley and St Pauls River Valley), two of which are consistent with previously reported glacial refugia. Chloroplast haplotype affinities provided evidence of migration of populations from the north and east towards the south and west of Tasmania. High nuclear microsatellite diversity was observed across the species’ range. Most of this variation was distributed within populations with low but significant FST, suggesting high gene flow among populations that is more pronounced in mature stands. Higher nuclear genetic diversity in newly colonised areas compared with lowland putative refugial regions, and the converse in chloroplast DNA markers, suggest limited seed dispersal into newly colonised regions combined with high pollen flow between different source populations in newly colonised areas. Our results do not support the suggestion that highland populations of E. pauciflora originate from in situ high-altitude refugia, but instead argue they originate from lowland refugia.

scholarly journals Molecular genetic diversity and relationship of indigenous sheep breeds of Pakistan based on nuclear microsatellite loci

2019 ◽  
Vol 30 (1) ◽  
pp. 54
Author(s):  
T Hussain ◽  
M M. Musthafa ◽  
M E. Babar ◽  
M Shaheen ◽  
F M. Marikar
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci ◽  
Molecular Genetic ◽  
Sheep Breeds ◽  
Indigenous Sheep ◽  
Molecular Genetic Diversity ◽  
Nuclear Microsatellite ◽  
Relationship Of

<p>En Pakistán los recursos genéticos ovinos son abundantes, disponiéndose de varias diferentes castas extendidas en todo el país. No obstante, la diversidad molecular de las razas de ovejas es poco conocida en la región. Por ello, en el presente estudio se investigaron 16 marcadores microsatelitales de las razas indígenas Buchi y Hashtnagri, en las provincias de Pendjab y Balochistan respectivamente. Para indagar la diversidad genética se obtuvieron muestras de sangre de 25 animales de cada raza. El promedio del número de alelos en Buchi y Hashtnagri fue de 3,375±1,455 y 3,50±1,591 respectivamente. Los promedios de heterocigosis registradas fueron de 0,878±0,204 para la raza Buchi y 0,885±0,218 para Hashtnagri. Los índices de Shannon fueron 1,032±0,371 para Buchi y 1,070±0,412 para Hashtnagri, respectivamente. La estimación de los coeficientes de endogamia (FIS y FIT) mostró valores negativos, en tanto que el flujo genético (migración) fue de 10,09 y el promedio de la diferencia poblacional (FST) resultó de 2,4%. En ambas razas, el índice de información polimórfica fue 0,56, indicando el valor del marcador del tablero. Entre las ovejas Buchi y Hashtnagri, la distancia genética estándar de Nei (Ds) fue 0,0218. Según estos resultados, ambas razas mostraron considerable diversidad genética. Los datos obtenidos permiten vislumbrar una promisoria mejoría en el área de la conservación y en el diseño de las estrategias para la cría de ovejas en el futuro cercano.</p>

Molecular genetic diversity of the French-American grapevine hybrids cultivated in North America

Genome ◽  
2003 ◽  
Vol 46 (6) ◽  
pp. 1037-1048 ◽  
Author(s):  
Patrick Pollefeys ◽  
Jean Bousquet
Keyword(s):  
Genetic Diversity ◽  
North America ◽  
Genetic Background ◽  
Molecular Genetic ◽  
Group Method ◽  
Dna Profile ◽  
Vitis Riparia ◽  
The North ◽  
Pair Group ◽  
Molecular Genetic Diversity

French-American hybrid grapevines are most popular in eastern and mid-western North America: they are hardy cultivars derived from crosses between the European Vitis vinifera and American wild vines. The aim of this study was to characterize their genetic background using 6 microsatellite (SSR) markers and a set of 33 diagnostic RAPD markers. The latter were reproducible with different PCR thermal cyclers. Two SSR loci were found to be synonymous, VrZAG47 and VVMD27. The DNA profile frequencies estimated for each cultivar were much lower with multi-locus SSR data than that obtained from multi-fragment RAPD data. There was no significant correlation between the multi-locus DNA profile frequencies derived from SSRs and those from RAPDs. Estimates of genetic diversity derived from SSRs were generally higher and the average similarity between cultivars was generally lower than values reported for subgroups of V. vinifera, in accordance with expectations for hybrid cultivars. The phenetic relationships depicted by UPGMA (unweighted pair-group method with arithmetic averaging) and neighbor-joining analyses of microsatellite data were congruent and, to a large extent, in agreement with the known pedigree or history of each cultivar. A major dichotomy was observed between one group where the known genetic background was dominated by the North American Vitis riparia and Vitis labrusca, and another one where the genetic background was dominated by the European V. vinifera. Two Kulhmann varieties thought to be synonymous were found to be different, though closely related.Key words: French-American hybrids, genetic diversity, RAPD, SSR, Vitis.

Molecular genetic diversity and population structure in Lycium accessions using SSR markers

2010 ◽  
Vol 333 (11-12) ◽  
pp. 793-800 ◽  
Author(s):  
Wei-Guo Zhao ◽  
Jong-Wook Chung ◽  
Young-Il Cho ◽  
Won-Hee Rha ◽  
Gi-An Lee ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Ssr Markers ◽  
Molecular Genetic ◽  
Molecular Genetic Diversity

scholarly journals Molecular genetic diversity and population structure of Ethiopian white lupin landraces: Implications for breeding and conservation

PLoS ONE ◽  
2017 ◽  
Vol 12 (11) ◽  
pp. e0188696 ◽  
Author(s):  
Mulugeta Atnaf ◽  
Nasser Yao ◽  
Kyalo Martina ◽  
Kifle Dagne ◽  
Dagne Wegary ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Molecular Genetic ◽  
White Lupin ◽  
Molecular Genetic Diversity

scholarly journals Molecular Genetic Diversity and Population Structure in Ethiopian Chickpea Germplasm Accessions

Diversity ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 247
Author(s):  
Tsegaye Getahun ◽  
Kassahun Tesfaye ◽  
Asnake Fikre ◽  
Teklehaimanot Haileslassie ◽  
Annapurna Chitikineni ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Ssr Markers ◽  
Molecular Genetic ◽  
Conservation Strategies ◽  
Molecular Genetic Diversity ◽  
Population Structure Analysis ◽  
Trait Improvement ◽  
Population Structures ◽  
Germplasm Accessions

Chickpea (Cicer arietinum L.) is a cheap source of protein and rich in minerals for people living in developing countries. In order to assess the existing molecular genetic diversity and determine population structures in selected Ethiopian chickpea germplasm accessions (118), a set of 46 simple sequence repeat (SSR) markers equally distributed on the chickpea genome were genotyped. A total of 572 alleles were detected from 46 SSR markers, and the number of alleles per locus varied from 2 (ICCM0289) to 28 (TA22). The average number of alleles per locus, polymorphism information content, and expected heterozygosity were 12, 0.684, and 0.699, respectively. Phylogenetic analysis grouped the 118 chickpea genotypes from diverse sources into three evolutionary and/or biological groups (improved desi, improved kabuli, and landraces). The population structure analysis revealed six sub-populations from 118 chickpea genotypes studied. AMOVA revealed that 57%, 29%, and 14% of the total genetic variations were observed among individuals, within populations, and among populations. The insights into the genetic diversity at molecular levels in the Ethiopian germplasm lines can be used for designing conservation strategies as well as the diverse germplasm lines identified in this study can be used for trait dissection and trait improvement.

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