A Label-Free Colorimetric Biosensor for 17β-Estradiol Detection Using Nanoparticles Assembled by Aptamer and Cationic Polymer

2016 ◽  
Vol 69 (1) ◽  
pp. 12 ◽  
Author(s):  
Dongwei Zhang ◽  
Weilin Zhang ◽  
Jiayun Ye ◽  
Shenshan Zhan ◽  
Bing Xia ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Detection Limit ◽  
Colorimetric Detection ◽  
Cationic Polymer ◽  
Label Free ◽  
Human Beings ◽  
Remarkable Change ◽  
Environment Effects ◽  
Diallyldimethylammonium Chloride ◽  
17Β Estradiol

Concern is mounting regarding the human health and environment effects of 17β-estradiol (E2), a natural oestrogen excreted by human beings and animals. In this paper, a sensitive and selective biosensor for the detection of E2 using gold nanoparticles (AuNPs), label-free E2-specific aptamer, and poly(diallyldimethylammonium chloride) (PDDA) was developed. In the absence of E2, PDDA can electrostatically interact with E2-specific aptamer, and the charge responsible for inducing AuNPs aggregation was destroyed. However, the introduction of E2 can specifically interact with the aptamer to form E2–aptamer complex so that PDDA can aggregate AuNPs and cause a remarkable change in colour from wine red to blue, which enables colorimetric detection of E2 with selectivity and a detection limit of 1.57 nM.

A Rapid and Sensitive Aptasensor for Cyromazine Detection in Raw Milk Based on a Nanogold Probe and G-Quadruplex Formation

2019 ◽  
Vol 72 (7) ◽  
pp. 555
Author(s):  
Xu Dang ◽  
Wenchao Gu ◽  
Xiyin Zheng ◽  
Xuelian Fei ◽  
Fuxiang Tian ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Limit Of Detection ◽  
Raw Milk ◽  
Cationic Polymer ◽  
Remarkable Change ◽  
Detection Range ◽  
Naked Eye ◽  
Diallyldimethylammonium Chloride ◽  
G Quadruplex ◽  
Quadruplex Formation

Herein, a rapid, facile, and colourimetric sensor for the detection of cyromazine in raw milk is reported using an aptamer based on gold nanoparticles (AuNPs). A sequence-specific aptamer for cyromazine called Tcyr1 is designed to absorb on the surface of AuNPs and electrostatically interacts with poly(diallyldimethylammonium chloride) (PDDA), which prevents AuNPs from aggregating. It can also self-assemble to form a G-quadruplex-CYR complex with cyromazine. Because of its specificity and stability, the introduction of cyromazine in raw milk would influence the protection thus the following cationic polymer could aggregate AuNPs and cause a remarkable change in colour. According to this, the presence of cyromazine can be determined by the naked eye and means of absorbance. This sensor is selective for the detection of cyromazine in raw milk and has a limit of detection of 200 ppb by the naked eye and of 5.8 ppb by spectrophotometer, and has a detection range from 0.1 to 1 ppm.

scholarly journals Disulfide-induced self-assembled targets: A novel strategy for the label free colorimetric detection of DNAs/RNAs via unmodified gold nanoparticles

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ehsan Shokri ◽  
Morteza Hosseini ◽  
Mehdi D. Davari ◽  
Mohammad R. Ganjali ◽  
Maikel P. Peppelenbosch ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Detection ◽  
Label Free ◽  
Self Assembled ◽  
Novel Strategy

scholarly journals Highly sensitive colorimetric detection of 17β-estradiol using split DNA aptamers immobilized on unmodified gold nanoparticles

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Jinchuan Liu ◽  
Wenhui Bai ◽  
Shucao Niu ◽  
Chao Zhu ◽  
Shuming Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Detection ◽  
Dna Aptamers ◽  
Highly Sensitive ◽  
17Β Estradiol

scholarly journals Enhancement of the Peroxidase-Like Activity of Iodine-Capped Gold Nanoparticles for the Colorimetric Detection of Biothiols

Biosensors ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 113 ◽  
Author(s):  
Chia-Chen Chang ◽  
Tsz-Lian Hsu ◽  
Chie-Pein Chen ◽  
Chen-Yu Chen
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Detection Limit ◽  
Enzymatic Activity ◽  
Catalytic Properties ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Synergetic Effect ◽  
Vis Spectroscopy ◽  
Wide Range

A colorimetric assay was developed for the detection of biothiols, based on the peroxidase-like activity of iodine-capped gold nanoparticles (AuNPs). These AuNPs show a synergetic effect in the form of peroxidase-mimicking activity at the interface of AuNPs, while free AuNPs and iodine alone have weak catalytic properties. Thus, iodine-capped AuNPs possess good intrinsic enzymatic activity and trigger the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB), leading to a change in color from colorless to yellow. When added to solution, biothiols, such as cysteine, strongly bind to the interface of AuNPs via gold-thiol bonds, inhibiting the catalytic activity of AuNPs, resulting in a decrease in oxidized TMB. Using this strategy, cysteine could be linearly determined, at a wide range of concentrations (0.5 to 20 μM), with a detection limit of 0.5 μM using UV-Vis spectroscopy. This method was applied for the detection of cysteine in diluted human urine.

Colorimetric Detection of Trace Arsenic(III) in Aqueous Solution Using Arsenic Aptamer and Gold Nanoparticles

2014 ◽  
Vol 67 (5) ◽  
pp. 813 ◽  
Author(s):  
Minglei Yu
Keyword(s):  
Aqueous Solution ◽  
Gold Nanoparticles ◽  
Detection Limit ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Colour Change ◽  
Colorimetric Sensing ◽  
Detection Range ◽  
Colorimetric Indicator ◽  
Blue Solution

In this study, trace arsenic(iii) (AsIII) in aqueous solution was detected by applying a classical aptamer-based gold nanoparticles colorimetric sensing strategy. An arsenic aptamer was used as a sensing probe and gold nanoparticles as a colorimetric indicator. In the absence of AsIII, the gold nanoparticles were stabilised by the arsenic aptamer and remained dispersed at high NaCl concentrations, displaying a red solution. Contrarily, in the presence of AsIII, the gold nanoparticles were prone to aggregation, owing to the formation of aptamer–AsIII complex between the arsenic aptamer and AsIII, and thus exhibited a blue solution. By monitoring the colour change, a simple and fast colorimetric assay for AsIII was established with a detection range of 1.26–200 ppb and a detection limit of 1.26 ppb. Because this colorimetric assay only involves common reagents and can be assessed visually, it holds great potential for arsenic(iii) monitoring in environment-related and other applications.

Colorimetric Detection of DNA Sequences Using an Organic Solvent to Induce the Aggregation of Label-Free Gold Nanoparticles

2013 ◽  
Vol 13 (6) ◽  
pp. 3805-3809 ◽  
Author(s):  
Huning Jia ◽  
Xiaomei Cao ◽  
Cong Fu ◽  
Jianping Wang ◽  
Zhiyao Chen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Organic Solvent ◽  
Dna Sequences ◽  
Colorimetric Detection ◽  
Label Free ◽  
Free Gold

Selection of a DNA aptamer for cadmium detection based on cationic polymer mediated aggregation of gold nanoparticles

The Analyst ◽  
2014 ◽  
Vol 139 (6) ◽  
pp. 1550-1561 ◽  
Author(s):  
Yuangen Wu ◽  
Shenshan Zhan ◽  
Lumei Wang ◽  
Pei Zhou
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Detection ◽  
Cationic Polymer ◽  
Dna Aptamer ◽  
Recognition Element ◽  
Cadmium Detection ◽  
Selection Of

The DNA aptamer toward cadmium was selected by a novel SELEX strategy and further considered as a recognition element for the colorimetric detection of Cd(ii).

Colorimetric detection of heparin with high sensitivity based on the aggregation of gold nanoparticles induced by polymer nanoparticles

2017 ◽  
Vol 41 (19) ◽  
pp. 10592-10597 ◽  
Author(s):  
Fei Qu ◽  
Yanqun Liu ◽  
Haili Lao ◽  
Yaping Wang ◽  
Jinmao You
Keyword(s):  
Gold Nanoparticles ◽  
Au Nanoparticles ◽  
Colorimetric Detection ◽  
High Sensitivity ◽  
Cationic Polymer ◽  
Polymer Nanoparticles

The negatively charged heparin hinders the aggregation of Au nanoparticles induced by the cationic polymer nanodots.

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