Effect of Modification Protocols on the Effectiveness of Gold Nanoparticles as Drug Delivery Vehicles for Killing of Breast Cancer Cells

The current study evaluated the potential of gold nanoparticles (AuNPs) for the delivery of Taxol to breast cancer cells (T47D) using an in vitro cell culture model. For this study, new loading approaches and novel chemical attachments were investigated. Five different gold nanoparticle-based complexes were used to determine their cytotoxicity towards T47D cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) viability assay. There was no significant decrease (P > 0.05) in cell viability when T47D cells were treated with AuNPs that did not contain Taxol. However, cells were significantly killed by gold nanoparticles chemically conjugated to Taxol using three different approaches and one novel hybrid AuNP-Taxol nanoparticle, wherein no chemical bonds were involved. These Taxol-loaded AuNPs were more effective at inducing cell death in vitro than a solution of free Taxol used to treat cells. This result demonstrated that Taxol could be released from the particles in the cell culture media for subsequent therapeutic action. Additionally, the experiments proved that the Taxol-loaded AuNPs were more toxic in a dose dependent manner than Taxol as a formulation for the treatment of breast cancer cells. The results of this study suggest that gold nanoparticles have potential for the efficient delivery of Taxol to breast cancer cells. This could provide a future solution as an alternative application method to overcome adverse side effects resulting from current high-dose treatment regimes.

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Impact of Morphine on Viability of MCF-7 and T47D Breast Cancer Cells

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Animal Science and Biotechnologies ◽

10.15835/buasvmcn-asb:0021.19 ◽

2019 ◽

Vol 76 (2) ◽

pp. 123

Author(s):

Ileana MICLEA ◽

Ana-Maria PUI ◽

Marius ZĂHAN

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Exposure Time ◽

Breast Cancer Cells ◽

Culture Media ◽

Colorimetric Method ◽

Breast Cancer Cell Lines ◽

T47d Cells ◽

Mcf 7

Morphine, a highly potent analgesic, is prescribed for the treatment of severe pain associated with cancer. Several in vitro and animal studies suggest that morphine is involved both in promoting and inhibiting tumor growth. Our aim was to test the outcome of adding morphine to the culture media of cells from two of the most widely used breast cancer cell lines. MCF-7 and T47D cells were seeded into 96-well microplates and cultured for 24 hours in MEM and RPMI-1640 media respectively. Afterwards, cells were exposed for 24, 48 or 72 hours to media containing morphine at the following concentrations: 0.05, 0.075, 0.1, 0.25, 0.5, 0.75, 1 μM. Cell viability was assessed by the MTT colorimetric method. After exposure of MCF-7 cells to morphine for 24 and 48 hours, viability was similar to the control while, after 72 hours, this parameter was significantly enhanced at 0.75 μM and 1 μM. Survival of T47D cells in the first 24 hours was significantly (p<0.05) increased by the presence of 1 μM morphine, while an increased exposure time did not improve the outcome. Our results show that morphine can increase viability of breast cancer cells, depending on concentration, exposure time and cell origin.

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Targeted metabolomics in the cell culture media reveals increased uptake of branched amino acids by breast cancer cells

Analytical Biochemistry ◽

10.1016/j.ab.2021.114192 ◽

2021 ◽

pp. 114192

Author(s):

Fang Kou ◽

Bangjie Zhu ◽

Wenbin Zhou ◽

Chunming Lv ◽

Yu Cheng ◽

...

Keyword(s):

Breast Cancer ◽

Amino Acids ◽

Cell Culture ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Culture Media ◽

Targeted Metabolomics ◽

Cell Culture Media ◽

Branched Amino Acids

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A New Highlight of Ephedra alata Decne Properties as Potential Adjuvant in Combination with Cisplatin to Induce Cell Death of 4T1 Breast Cancer Cells In Vitro and In Vivo

Cells ◽

10.3390/cells9020362 ◽

2020 ◽

Vol 9 (2) ◽

pp. 362 ◽

Cited By ~ 6

Author(s):

Fairouz Sioud ◽

Souheila Amor ◽

Imène ben Toumia ◽

Aida Lahmar ◽

Virginie Aires ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Protective Action ◽

Dependent Manner ◽

4T1 Breast Cancer ◽

Induced Apoptosis ◽

Cellular Modifications

Despite major advances in the last 10 years, whether in terms of prevention or treatment, the 5 year survival rate remains relatively low for a large number of cancers. These therapeutic failures can be the consequence of several factors associated with the cellular modifications or with the host by itself, especially for some anticancer drugs such as cisplatin, which induces a nephrotoxicity. In the strategy of research for active molecules capable both of exerting a protective action against the deleterious effects of cisplatin and exerting a chemosensitizing action with regard to cancer cells, we tested the potential effects of Ephedra alata Decne extract (E.A.) rich in polyphenolic compounds towards a 4T1 breast cancer model in vitro and in vivo. We showed that E.A. extract inhibited cell viability of 4T1 breast cancer cells and induced apoptosis in a caspase-dependent manner, which involved intrinsic pathways. Very interestingly, we observed a synergic antiproliferative and pro-apoptotic action with cisplatin. These events were associated with a strong decrease of breast tumor growth in mice treated with an E.A./cisplatin combination and simultaneously with a decrease of hepato- and nephrotoxicities of cisplatin.

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Toxicological Effects of Traumatic Acid and Selected Herbicides on Human Breast Cancer Cells: In Vitro Cytotoxicity Assessment of Analyzed Compounds

Molecules ◽

10.3390/molecules24091710 ◽

2019 ◽

Vol 24 (9) ◽

pp. 1710 ◽

Cited By ~ 4

Author(s):

Agata Jabłońska-Trypuć ◽

Urszula Wydro ◽

Elżbieta Wołejko ◽

Andrzej Butarewicz

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Breast Cancer Cell Lines ◽

Dependent Manner ◽

Food Contaminants ◽

Food Ingredient ◽

Concentration Dependent Manner ◽

Traumatic Acid

The main consequence of herbicides use is the presence of their residues in food of plant origin. A growing body of evidence indicates that herbicides cause detrimental effects upon human health while demonstrating a direct link of pesticides exposure with the occurrence of human chronic diseases, including cancer. There is a pressing need to develop our knowledge regarding interactions of food contaminants and food components both in vitro and in vivo. Pesticides are highly undesirable food contaminants, and traumatic acid (TA) is a very beneficial food ingredient, therefore we decided to study if TA may act as a compound that delays the stimulatory effect of pesticides on breast cancer cells. To analyze the potential effects that selected herbicides (MCPA, mesotrione, bifenox and dichlobenil) may have upon cancerous cells, we conducted studies of the cytotoxicity of physiological concentrations of four pesticides and the mix of TA with tested herbicides in three different breast cancer cell lines (MCF-7, ZR-75-1 and MDA-MB-231) and one normal healthy breast cell line MCF-12A. Based on the obtained results we conclude that TA in a concentration-dependent manner might influence selected effects of the studied herbicides for particular cancer cells lines.

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VP-128, a novel oestradiol-platinum(II) hybrid with selective anti-tumour activity towards hormone-dependent breast cancer cells in vivo

Endocrine Related Cancer ◽

10.1677/erc-09-0113 ◽

2009 ◽

Vol 16 (4) ◽

pp. 1185-1195 ◽

Cited By ~ 29

Author(s):

Céline Van Themsche ◽

Sophie Parent ◽

Valérie Leblanc ◽

Caroline Descôteaux ◽

Anne-Marie Simard ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Nuclear Accumulation ◽

Dependent Manner ◽

Anti Tumour Activity ◽

Tumour Activity ◽

Mcf 7

We have previously reported the synthesis of VP-128, a new 17β-oestradiol (E2)-linked platinum(II) hybrid with high affinity for oestrogen receptor α (ERα). In the present study, we have investigated the anti-tumour activity of VP-128 towards breast cancer cells in vitro and in vivo. We used human ERα-positive (MCF-7) and -negative (MDA-MB-468) cells as a model for treatment with increasing doses of VP-128, cisplatin or E2 in vitro and for xenograft experiments in nude mice in vivo. Compared with cisplatin, VP-128 showed markedly improved in vitro and in vivo anti-tumour activity towards ERα-positive MCF-7 breast cancer cells, without increased systemic toxicity. In these caspase-3-deficient cells, treatment with VP-128 overcame weak cellular sensitivity to cisplatin in vitro and in vivo. In these cells, only the hybrid induced apoptosis in an ERα-dependent manner, inactivated both X-linked inhibitor of apoptosis protein and Akt, and induced selective nuclear accumulation of ERα and the expression of ER-regulated genes c-myc and tff1, which was blocked by ERα-specific antagonist ICI 282 780. In the case of ERα-negative MDA-MB-468 cells, VP-128, but not cisplatin, induced nuclear accumulation of apoptosis-inducing factor and inhibited c-myc expression. However, VP-128 did not show enhanced in vivo anti-tumour activity compared with cisplatin. These results reveal two different modes of action for VP-128 in ERα-positive and -negative breast cancer cells, and highlight the promising therapeutic value of this unique E2-platinum hybrid for selective targeting of hormone-dependent cancers.

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SAT-124 Optimization of Experimental Conditions for Mimicking Hypoxia in Cultured Breast Cancer Cells by Using Cobalt(II) Chloride (CoCl2)

Journal of the Endocrine Society ◽

10.1210/jendso/bvaa046.1318 ◽

2020 ◽

Vol 4 (Supplement_1) ◽

Author(s):

Karin Chen ◽

Leo Satlof ◽

Udithi Kothapalli ◽

Noah Ziluck ◽

Maribel Lema ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Cell Apoptosis ◽

Breast Cancer Cells ◽

Nuclear Translocation ◽

Breast Cancer Cell Lines ◽

Mrna Levels ◽

Dependent Manner ◽

Experimental Conditions

Abstract Hypoxia is a common phenomenon in solid tumor development caused by a decrease in either oxygen concentration or oxygen pressure as a result of rapid tumor cell growth. Hypoxia is characterized by stabilization of the alpha subunit of the hypoxia-inducible factor (HIF-1α) and its nuclear translocation and heterodimerization with HIF-1β. Activation of this signaling pathway involves multiple downstream effectors including carbonic anhydrase 9 (CA9, s. CAIX). A reliable method to mimic hypoxia utilizes cobalt(II) chloride (CoCl2), which directly induces the expression of HIF-1α. The aim of this study was to optimize the experimental conditions for CoCl2 treatment of breast cancer cells in vitro using three human breast cancer cell lines (MDA-MB-231, T-47D, and MCF-7 cells). We performed time- and concentration-response experiments, using various concentrations of CoCl2 (50, 100, 200, and 300 μM) for 24 and 48 hours, and measured the expression of HIF-1α and CA9 by qRT-PCR and Western blot analyses. Results demonstrated that CoCl2 downregulated HIF-1α mRNA levels but upregulated CA9 mRNA levels in a concentration- and time-dependent manner. Concomitantly, CoCl2 treatment resulted in a significant induction of HIF-1α protein levels. We further investigated the effect of the CoCl2 concentrations listed above on cell apoptosis using an in situ apoptosis detection kit. The results demonstrated that concentrations of CoCl2 up to 100 μM had no significant effect on cell apoptosis.

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PFKFB4 Promotes Breast Cancer Metastasis via Induction of Hyaluronan Production in a p38-Dependent Manner

Cellular Physiology and Biochemistry ◽

10.1159/000495055 ◽

2018 ◽

Vol 50 (6) ◽

pp. 2108-2123 ◽

Cited By ~ 3

Author(s):

Ruifang Gao ◽

Yanhua Liu ◽

Dan Li ◽

Jing Xun ◽

Wei Zhou ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Breast Cancer Cells ◽

Tumor Metastasis ◽

Dependent Manner ◽

Fructose 2

Background/Aims: The bi-functional enzyme 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase-4 (PFKFB4) is highly expressed in many types of cancer and its requirement for tumor survival has been demonstrated in glioma, lung, and prostate cancers. However, whether PFKFB4 plays a role in the tumor metastasis remains uncertain. This study explores the role of PFKFB4 in tumor metastasis and its underlying mechanisms in breast cancer cells. Methods: The expression of PFKFB4 was first analyzed using the Cancer Genome Atlas (TCGA) dataset, and confirmed by immunohistochemical staining of tissue microarray and breast cancer tissues from patient samples. Gain- and loss-of- function approaches were used to investigate the effects of PFKFB4 on breast cancer cell migration in vitro. Orthotopic xenograft model and experimental metastasis model were used to assess the effects of PFKFB4 on breast cancer cell metastasis in vivo. ELISA and immunofluorescence staining were used to examine HA production. Quantitative RT-PCR and western blotting were used to explore the mRNA and protein levels of HAS2, respectively. Results: We found that PFKFB4 enhances the migration/invasiveness of breast cancer cells in vitro as well as in vivo. Notably, the effects of PFKFB4 on migration are mediated by induction of HAS2 expression and HA production. Moreover, PFKFB4-induced HAS2 up-regulation depends upon the activation of p38 signaling. Conclusion: PFKFB4 promotes the metastasis of breast cancer cells via induction of HAS2 expression and HA production in a p38-dependent manner. Therefore, the PFKFB4/p38/HAS2 signaling pathway may serve as a potential therapeutic target for metastatic breast cancer.

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The potency of Pentagamavunone‐0 (PGV‐0) as chemopreventive agent for the formation and growth of breast cancer as revealed in 3D model

Indonesian Journal of Biotechnology ◽

10.22146/ijbiotech.51759 ◽

2020 ◽

Vol 25 (1) ◽

pp. 21

Author(s):

Wulandari Wulandari ◽

Muthi’ Ikawati ◽

Edy Meiyanto

Keyword(s):

Breast Cancer ◽

Cell Culture ◽

Anticancer Activity ◽

Cancer Cells ◽

Breast Cancer Cells ◽

3D Model ◽

3D Culture ◽

Cancer Cell Line ◽

Dependent Manner ◽

Chemopreventive Agent

Pentagamavunone‐0 (PGV‐0) or 2,5‐bis(4’‐hydroxy‐3‐methoxybenzylidine)‐cyclopentanone is a curcumin analogue that exhibits anticancer activity in breast cancer cells. However, most of previous reports are limited to the use of two‐dimensional (2D) cell culture. The use of three‐dimensional (3D) cell culture model in cancer research can represent the real condition of cancer growth in patients better than the 2D culture. The purpose of this study was to determine the anticancer activity of PGV‐0 on a 3D model of HCC 1954 breast cancer cells. HCC 1954 cells were grown in the 3D culture in the presence of PGV‐0, and the spheroid formation and growth of formed spheroids were observed using microscope at 24 and 96 h, respectively. The cytotoxic effects were measured by MTT assay. PGV‐0 inhibited the formation and growth of spheroids at the concentration as low as 60 µM. The cytotoxic effect of PGV‐0 appeared in a dose‐dependent manner with the IC50 value of 70.9 µM. The results of this study indicate that PGV‐0 has an anticancer activity on a 3D model of HCC 1954 breast cancer cell line. Therefore, the result supported the potency of PGV‐0 as cancer chemopreventive agent.

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