scholarly journals Associative diazotrophic bacteria from forage grasses in the Brazilian semi-arid region are effective plant growth promoters

2019 ◽  
Vol 70 (10) ◽  
pp. 899 ◽  
Author(s):  
Gabiane dos Reis Antunes ◽  
Sheilla Rios Assis Santana ◽  
Indra Elena Costa Escobar ◽  
Marivaine da Silva Brasil ◽  
Gherman Garcia Leal de Araújo ◽  
...  
Keyword(s):  
Plant Growth ◽  
Arid Region ◽  
Growth Promotion ◽  
Nifh Gene ◽  
Forage Grasses ◽  
Bacterial Isolates ◽  
Diazotrophic Bacteria ◽  
Semi Arid Region ◽  
Semi Arid

The study of plant growth-promoting bacteria (PGPB) can identify outstanding bacteria for crops. For forage grasses adapted to drylands, the selection of PGPB can increase the field performance of pastures. The aim of this study was to isolate, and characterise at molecular, biochemical and symbiotic levels, diazotrophic bacteria obtained from buffel grass (Cenchrus ciliaris), sorghum (Sorghum bicolor) and Tifton 85 (Cynodon spp.) from Brazilian semi-arid region fields. Field-grown plants were collected, and the roots were surface-disinfected, crushed and inoculated in a semi-solid medium. After the formation and confirmation of microaerophilic pellicles, the bacteria were isolated and purified. All bacterial isolates were subjected to nifH gene amplification and identified by their partial 16S rRNA gene sequences. The bacteria were evaluated for the production of auxins and siderophores, calcium phosphate solubilisation, and diazotrophic ability as ‘in vitro’ plant growth-promotion traits. A plant inoculation assay was conducted to assess the plant growth-promotion abilities of the bacterial isolates. Twenty-one bacterial isolates harboured the nifH gene (nifH+), among which nine were obtained from sorghum, eight from buffel grass, and four from Tifton 85. The bacterial isolates were classified as Bacillus (8), Stenotrophomonas (7), Agrobacterium (4), Cellulomonas (1) and Paenibacillus (1). All were shown to be auxin producers, with 14 isolates showing diazotrophic capacity ‘in vitro’. Fourteen isolates increased plant N content, but the bacterial strains ESA 392 and ESA 398 (Bacillus), ESA 397 and ESA 407 (Stenotrophomonas), and ESA 401 (Agrobacterium) were shown to promote both plant growth and N nutrition. These strains are candidates for further assays to evaluate their agronomic performance under field conditions, aiming inoculant production for forage grasses in drylands.

Isolation and characterization of plant growth-promoting rhizobacteria from Opuntia ficus-indica and their effect on Wheat growth

2020 ◽  
Vol 9 (6) ◽  
pp. 255-265
Author(s):  
Draou N ◽  
Selami N ◽  
Karkachi N ◽  
Kacem Mourad ◽  
Kaid Harche M
Keyword(s):  
Plant Growth ◽  
Arid Region ◽  
Antagonistic Effect ◽  
Phytopathogenic Fungus ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Isolation And Characterization ◽  
Semi Arid Region ◽  
Semi Arid

Endophytic bacteria are found inside host plants without causing disease symptoms, and form a range of di erent bene cial rela onships. In this study, four bacterial strains were isolated from roots, cladodes and non gus- tatory fruits of Opun a cus-indicaL. collected from the Algerian semi-arid region. The bacteria were able to grow at temperatures as 45 °C. 16SrRNA sequencing revealed the iden ty of isolates as Pseudomonas pu da(E1)with 98% similarity,P. brassicacearum(E2) and Klebsiella pneumonia (E3,E4)with 99% similarity. The strains were tested in vitro for their biocontrol ac vity against phytopathogenic fungus, and found to have the ability to inhibit the Fusarium oxysporum f. sp. albedinis and Aspergillus niger growth. We noticed that the most important inhibitory effect recorded with P. pu da (69.78±3.93 and 65.15±2.8), and that the feeble antagonistic effect recorded with P. brassicacearum (20.57±6.04 and 8.52 ±2.8) against of F.oxysporum f.sp albdeinis and A.niger. The plant growth promo ng (PGP) proper es were demonstrated through wheat (Triticum aestivum L.) based bioassay under hydroponic condi ons.The inocula on of bacteria showed an increase in density, length of roots and stems, indica ng the PGP abili es of these bacteria. P. pu da showed a poten al for growth of wheat plant.We no ced respectively the length of both stems and roots (17.34 ± 0.54 mm; 11.07 ± 0.67 mm), and we found that fresh and dry weight was (0.23 ± 0.029 g; 0.0375 ±0.003 g). Overall the results showed that isolates characterized were able to improve the growth of plants, and inhibit the propaga on of phytopathogenic fungi. To our knowledge, this is the rst report describing the isola on of bacteria from O. cus-indica(L.) in Algerian semi-arid region.

scholarly journals Multifarious Indigenous Diazotrophic Rhizobacteria of Rice (Oryza sativa L.) Rhizosphere and Their Effect on Plant Growth Promotion

2022 ◽  
Vol 8 ◽  
Author(s):  
Mohammad Imran Mir ◽  
Bee Hameeda ◽  
Humera Quadriya ◽  
B. Kiran Kumar ◽  
Noshin Ilyas ◽  
...  
Keyword(s):  
Plant Growth ◽  
Phosphate Solubilization ◽  
Nitrogenase Activity ◽  
Hydrolytic Enzymes ◽  
Seed Vigor ◽  
Bacterial Isolates ◽  
Diazotrophic Bacteria ◽  
Pgp Traits ◽  
Rice Plants

A diverse group of rhizobacteria persists in the rhizospheric soil, on the surface of roots, or in association with rice plants. These bacteria colonize plant root systems, enhance plant growth and crop yield. Indigenous rhizobacteria are known to promote soil health, grain production quality and serve as sustainable bioinoculant. The present study was aimed to isolate, identify and characterize indigenous plant growth promoting (PGP) diazotrophic bacteria associated with the rhizosphere of rice fields from different areas of Jammu and Kashmir, India. A total of 15 bacteria were isolated and evaluated for various PGP traits, antagonistic activity against phytopathogens, production of hydrolytic enzymes and biofilm formation under in-vitro conditions. The majority of the isolated bacteria were Gram-negative. Out of 15 bacterial isolates, nine isolates produced IAA (12.24 ± 2.86 to 250.3 ± 1.15 μg/ml), 6 isolates exhibited phosphate solubilization activity (36.69 ± 1.63 to 312.4 ± 1.15 μg/ml), 7 isolates exhibited rock phosphate solubilization while 5 isolates solubilized zinc (10–18 mm), 7 isolates showed siderophore production, 8 isolates exhibited HCN production, 6 isolates exhibited aminocyclopropane-1-carboxylate (ACC) deaminase activity, 13 isolates exhibited cellulase activity, nine isolates exhibited amylase and lipase activity and six isolates exhibited chitinase activity. In addition, 5 isolates showed amplification with the nifH gene and showed a significant amount of nitrogenase activity in a range of 0.127–4.39 μmol C2H4/mg protein/h. Five isolates viz., IHK-1, IHK-3, IHK-13, IHK-15 and IHK-25 exhibited most PGP attributes and successfully limited the mycelial growth of Rhizoctonia solani and Fusarium oxysporum in-vitro. All the five bacterial isolates were identified based on morphological, biochemical and 16S rDNA gene sequencing study, as Stenotrophomonas maltophilia, Enterobacter sp., Bacillus sp., Ochrobactrum haematophilum and Pseudomonas aeruginosa. Rice plants developed from seeds inoculated with these PGP strains individually had considerably higher germination percentage, seed vigor index and total dry biomass when compared to control. These findings strongly imply that the PGP diazotrophic bacteria identified in this work could be employed as plant growth stimulators in rice.

scholarly journals Exploitation of endophytic Pseudomonas sp. for plant growth promotion and colonization in rice

2017 ◽  
Vol 9 (3) ◽  
pp. 1310-1316
Author(s):  
Gurjot Kaur ◽  
Poonam Sharma ◽  
Deepika Chhabra ◽  
Kailash Chand ◽  
Gurjit Singh Mangat
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Pseudomonas Sp ◽  
Bacterial Isolates ◽  
Pgp Traits ◽  
P Solubilization ◽  
Fluorescent Pigment

The present investigation was carried out to exploit bacterial endophytes associated with root and leaf tissue of rice plant for plant growth promotion (PGP) and colonization study in vitro. Total 10 endophytic bacterial isolates (Pseudomonas sp.) were evaluate for PGP traits like P solubilization, production of Indole acetic acid (IAA), siderophore, ACC deaminase, protease, cellulase, fluorescent pigment, urease and denitrification activity. Out of 10 endophytic bacteria 30 %, 60 %, 20 %, 70 %, 10 % and 10 % were positive for siderophore, protease, cellulase, fluorescent pigment, urease and denitrification respectively. Maximum IAA production was recorded with isolate LRBLE7 (18.8 μgml-1) followed by LRBRE4 (16.0 μgml-1) and maximum P-solubilization was recorded with isolate LRBRE4 (5.8 mg 100 ml-1) followed by LRBLE7 (4.4 mg 100 ml-1). ACC deaminase production was recorded with isolate LRBLE6 (O.D=0.352 nm) followed by LRBRE5 (O.D=0.324nm). Three potential isolates (LRBRE4, LRBRE6 and LRBLE7) were selected on the basis of multiple PGP traits and were subjected to colonization study of rice seedling in vitro. Potential bacterial isolates can be exploited for improving growth and productivity in rice under sustainable management system.

scholarly journals In vitro antagonistic potential, plant growth-promoting activity and indole-3-acetic acid producing trait of bacterial isolates from spent mushroom substrate of Agaricus bisporus

2020 ◽  
Vol 25 (2) ◽  
pp. 22
Author(s):  
Rethinasamy Velazhahan ◽  
Shima Nasser Hamed Al-Mamari ◽  
Abdullah Mohammed Al-Sadi ◽  
Issa Hashil Al-Mahmooli ◽  
S. P. Sathish Babu
Keyword(s):  
Plant Growth ◽  
Agaricus Bisporus ◽  
Growth Promotion ◽  
Inhibition Zone ◽  
Bacterial Isolates ◽  
Plant Growth Promoting ◽  
Antagonistic Potential ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

Spent mushroom substrate (SMS) is widely used as fertilizer and to control plant diseases. The microorganisms surviving in SMS play a crucial role in plant growth promotion and biocontrol properties of SMS. In this study, an effort was made to isolate and characterize the bacterial species present in the SMS of Agaricus bisporus and to study their antagonistic potential, plant growth-promoting ability and indole-3-acetic acid (IAA) producing trait. Six different bacterial isolates exhibiting morphological variabilities were obtained from the SMS by serial dilution technique. On the basis of 16S rRNA gene sequences, these isolates were identified as Staphylococcus epidermidis (Sh1 and Sh3), S. aureus (Sh2), Bacillus albus (Sh4), Delftia lacustris (Sh6) and Comamonas aquatica (Sh7). These bacterial strains were assayed for their antagonism against Pythium aphanidermatum, a phytopathogenic oomycete. The results of in vitro dual culture assay revealed that all the 6 bacterial isolates showed low levels of suppression of P. aphanidermatum and recorded less than 5 mm inhibition zone. Among the bacterial isolates, S. epidermidis Sh3 recorded the maximum inhibition zone of 4.2 mm. Plant growth promotion test using roll paper towel method revealed that C. aquatica Sh7, B. albus Sh4, D. lacustris Sh6 and S. epidermidis Sh3 caused a significant increase in seedling vigour of cucumber compared to control. The seeds treated with the bacterial isolate C. aquatica Sh7 showed the maximum seedling vigor. Assessment of in vitro production of IAA by the bacterial isolates revealed that the bacterial isolates highly varied (ranging from 0.28 to 9.25 mg L-1) in their potential for production of IAA. The maximum amount of IAA was produced by C. aquatica Sh7 (9.25 mg L-1), while the minimum was produced by S. epidermidis Sh1 (0.28 mg L-1).

scholarly journals Characterization ofPseudomonasSpecies Isolated from the Rhizosphere of Plants Grown in Serozem Soil, Semi-Arid Region of Uzbekistan

2005 ◽  
Vol 5 ◽  
pp. 501-509 ◽  
Author(s):  
Dilfuza Egamberdiyeva
Keyword(s):  
Arid Region ◽  
Pathogenic Fungi ◽  
Plant Pathogenic Fungi ◽  
Salt Tolerant ◽  
Pseudomonas Spp ◽  
Pot Experiments ◽  
Pseudomonas Alcaligenes ◽  
Semi Arid Region ◽  
Semi Arid

Collections of nativePseudomonasspp. are kept at the NCAM of Uzbekistan. Some of those organisms were isolated from the rhizosphere of cotton, wheat, corn, melon, alfalfa, and tomato grown in field locations within a semi-arid region of Uzbekistan. Strains used for this study were Pseudomonas alcaligenes,P. aurantiaca,P. aureofaciens,P. denitrificans,P. mendocina,P. rathonis, andP. stutzeri. Some of the pseudomonads have been characterized in this report. These strains produced enzymes, phytohormone auxin (IAA), and were antagonist against plant pathogenic fungi inin vitroexperiments. Most of the strains were salt tolerant and temperature resistant. Some of thePseudomonas spp. isolated in this study have been found to increase the growth of wheat, corn, and tomato in pot experiments.

scholarly journals Diazotrophic bacteria from maize exhibit multifaceted plant growth promotion traits in multiple hosts

2020 ◽  
Author(s):  
Shawn M. Higdon ◽  
Tania Pozzo ◽  
Emily J. Tibbett ◽  
Colleen Chiu ◽  
Richard Jeannotte ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Genomic Analysis ◽  
Comparative Genomic ◽  
Marker Genes ◽  
Diazotrophic Bacteria ◽  
In Planta ◽  
Pgp Traits

AbstractSierra Mixe maize is a geographically remote landrace variety grown on nitrogen-deficient fields in Oaxaca, Mexico that meets its nutritional requirements without synthetic fertilizer by associating with free-living diazotrophs comprising the microbiota of its aerial root mucilage. We selected nearly 500 diazotrophic bacteria isolated from Sierra Mixe maize mucilage and sequenced their genomes. Comparative genomic analysis demonstrated that isolates represented diverse genera and possessed multiple marker genes for mechanisms of direct plant growth promotion (PGP). In addition to nitrogen fixation, we examined deamination of 1-amino-1-cyclopropanecarboxylic acid, biosynthesis of indole-3-acetic acid, and phosphate solubilization. Implementing in vitro colorimetric assays revealed each isolate’s potential to confer the alternative PGP activities that corroborated genotype and pathway content. We examined the ability of mucilage diazotrophs to confer PGP by direct inoculation of clonally propagated potato plants in planta, which led to the identification of bio-stimulant candidates that were tested for PGP by inoculating a conventional maize variety. The results indicate that, while many diazotrophic isolates from Sierra Mixe maize possessed genotypes and in vitro phenotypes for targeted PGP traits, a subset of these organisms promoted the growth of potato and conventional maize using multiple promotion mechanisms.

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