The RAD51 gene family in bread wheat is highly conserved across eukaryotes, with RAD51A upregulated during early meiosis

2008 ◽  
Vol 35 (12) ◽  
pp. 1267 ◽  
Author(s):  
Kelvin H. P. Khoo ◽  
Hayley R. Jolly ◽  
Jason A. Able
Keyword(s):  
Gene Family ◽  
Bread Wheat ◽  
Higher Plants ◽  
Triticum Aestivum L ◽  
Chromosome Group ◽  
Rad51 Gene ◽  
Homology Searching ◽  
Structural Conservation ◽  
Group 2 ◽  
High Level

The RADiation sensitive protein 51 (RAD51) recombinase is a eukaryotic homologue of the bacterial Recombinase A (RecA). It is required for homologous recombination of DNA during meiosis where it plays a role in processes such as homology searching and strand invasion. RAD51 is well conserved in eukaryotes with as many as four paralogues identified in vertebrates and some higher plants. Here we report the isolation and preliminary characterisation of four RAD51 gene family members in hexaploid (bread) wheat (Triticum aestivum L.). RAD51A1, RAD51A2 and RAD51D were located on chromosome group 7, and RAD51C was on chromosome group 2. Q-PCR gene expression profiling revealed that RAD51A1 was upregulated during meiosis with lower expression levels seen in mitotic tissue, and bioinformatics analysis demonstrated the evolutionary linkages of this gene family to other eukaryotic RAD51 sequences. Western blot analysis of heterologously expressed RAD51 from bread wheat has shown that it is detectable using anti-human RAD51 antibodies and that molecular modelling of the same protein revealed structural conservation when compared with yeast, human, Arabidopsis and maize RAD51A orthologues. This report has widened the knowledge base of this important protein family in plants, and highlighted the high level of structural conservation among RAD51 proteins from various species.

Protein disulphide isomerase family in bread wheat (Triticum aestivum L.): genomic structure, synteny conservation and phylogenetic analysis

2011 ◽  
Vol 9 (2) ◽  
pp. 342-346 ◽  
Author(s):  
E. d'Aloisio ◽  
A. R. Paolacci ◽  
A. P. Dhanapal ◽  
O. A. Tanzarella ◽  
E. Porceddu ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Gene Family ◽  
Bread Wheat ◽  
Plant Species ◽  
Genomic Structure ◽  
Triticum Aestivum L ◽  
Protein Disulphide Isomerase ◽  
Homoeologous Genes ◽  
Genes Encoding ◽  
High Level

Eight genes encoding protein disulphide isomerase (PDI)-like proteins in bread wheat were cloned and characterized and their genomic structure was compared with that of homoeologous genes isolated from other plant species. Fourteen wheat cDNA sequences of PDI-like genes were amplified and cloned; eight of them were relative to distinct PDI-like genes, whereas six corresponded to homoeologous sequences. Also, the genomic sequences of the eight non-homoeologous genes were amplified and cloned. Phylogenetic analysis, which included eight genes encoding PDI-like proteins and the gene encoding the typical PDI, assigned at least one of them to each of the eight major clades identified in the phylogenetic tree of the PDI gene family of plants. The close chromosome synteny between wheat and rice was confirmed by the location of the homoeologous genes of the PDI family in syntenic regions of the two species. Within the same phylogenetic group, a high level of conservation, in terms of sequence homology, genomic structure and domain organization, was detected between wheat and the other plant species. The high level of conservation of sequence and genomic organization within the PDI gene family, even between distant plant species, might be ascribed to the key metabolic roles of their protein products.

scholarly journals High level of conservation between genes coding for the GAMYB transcription factor in barley (Hordeum vulgare L.) and bread wheat (Triticum aestivum L.) collections

2008 ◽  
Vol 117 (3) ◽  
pp. 321-331 ◽  
Author(s):  
Grit Haseneyer ◽  
Catherine Ravel ◽  
Mireille Dardevet ◽  
François Balfourier ◽  
Pierre Sourdille ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Transcription Factor ◽  
Hordeum Vulgare ◽  
Bread Wheat ◽  
Triticum Aestivum L ◽  
Hordeum Vulgare L ◽  
High Level

Molecular mapping of wheat. Homoeologous group 2

Genome ◽  
1995 ◽  
Vol 38 (3) ◽  
pp. 516-524 ◽  
Author(s):  
James C. Nelson ◽  
Allen E. Van Deynze ◽  
Mark E. Sorrells ◽  
Enrique Autrique ◽  
Yun Hai Lu ◽  
...  
Keyword(s):  
Bread Wheat ◽  
Molecular Mapping ◽  
Triticum Aestivum L ◽  
Homoeologous Group ◽  
Hordeum Vulgare L ◽  
Crop Species ◽  
D Genome ◽  
Wheat Group ◽  
Group 2 ◽  
Stem Rust Resistance Genes

A molecular-marker map of bread wheat having many markers in common with other grasses in the Gramineae family is a prerequisite for molecular level genetic studies and breeding in this crop species. We have constructed restriction fragment length polymorphism maps of the A-, B-, and D-genome chromosomes of homoeologous group 2 of hexaploid wheat (Triticum aestivum L. em. Thell) using 114 F7 lines from a synthetic × bread wheat cross and clones from 11 libraries. Chromosomes 2A, 2B, and 2D comprise 57, 60, and 56 markers and each spans about 200 cM. Comparisons between chromosomes are facilitated by 26 sets of homoeoloci. Genes mapped include a heterologous abscisic acid responsive locus cloned as pBS128, the epidermal waxiness inhibitor W21, and two presumed leaf rust and stem rust resistance genes. Anomalies suggesting ancestral rearrangements in chromosome 2B are pointed out and features of wheat group 2 chromosomes that are common to barley (Hordeum vulgare L.), rice (Oryza spp.), and T. tauschii are discussed.Key words: RFLP, wheat, waxy, rust.

scholarly journals Genetic variability assessment in bread wheat (Triticum aestivum) grown in Algeria using microsatellites SSR markers

2020 ◽  
Vol 21 (6) ◽  
Author(s):  
KARIMA KARA ◽  
MALIKA RACHED-KANOUNI ◽  
SAMAH MNASRI ◽  
Hichem Khammar ◽  
M’BAREK BEN NACEUR
Keyword(s):  
Triticum Aestivum ◽  
Genetic Variability ◽  
Bread Wheat ◽  
Ssr Markers ◽  
Genetic Similarity ◽  
Triticum Aestivum L ◽  
Breeding Programs ◽  
High Level ◽  
Derived Data ◽  
Selection Of

Abstract. Kara K, Rached-Kanouni M, Mnasri S, Khammar H, Ben Naceur MB. 2020. Genetic variability assessment in bread wheat (Triticum aestivum) grown in Algeria using microsatellites SSR markers. Biodiversitas 21: 2638-2644. The assessment of genetic diversity is a key step in evaluating the adaptation of populations to new environmental conditions, and thus in the selection of new cultivars. The present investigation aimed to assess genetic variability of bread wheat (Triticum aestivum L.) genotypes grown in Algeria. The 17 hexaploid genotypes of bread wheat were assessed using 16 molecular microsatellites of SSR (Simple Sequence Repeat). Among the 16 microsatellite markers tested, only 11 markers were the most polymorphic and reproducible. The Polymorphism Information Content (PIC) values per locus varied from 0.14 to 0.70 with an average of 0.48 and 0.49. Genetic similarity between genotypes varied from 0.27 and 0.92 with an average of 0.60. The highest genetic distance value of 0.92 has been scored between Kauz/Pastor/Fiscal and Wbll1*2/Brambling. The lowest value of 0.27 has been scored between Cham6 and Pastor/Wbll1. Genetic similarity was calculated by molecular derived data and used to produce a dendrogram. The genotypes were grouped in two clear clusters according to their origin and pedigree. The first cluster included wheat genotypes Ain Abid (local genotypes), Attila/2 Pastor, and 5119 (introduced genotypes). The second group revealed high polymorphism and was subdivided into four sub-groups. This high level of diversity revealed among the accessions of wheat, grown in Algeria could be used in breeding programs.

scholarly journals Molecular Similarity Relationships Among Iranian Bread Wheat Cultivars and Breeding Lines Using ISSR Markers

2012 ◽  
Vol 40 (2) ◽  
pp. 254 ◽  
Author(s):  
Reza DARVISHZADEH ◽  
Iraj BERNOUSI
Keyword(s):  
Bread Wheat ◽  
Geographic Origin ◽  
Triticum Aestivum L ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Wheat Breeding ◽  
Dice Similarity Coefficient ◽  
Breeding Lines ◽  
Information Index ◽  
High Level

Inter simple sequence repeat (ISSR) markers were used to characterize and assess genetic diversity of Iranian bread wheat (Triticum aestivum L.) using 101 cultivars and breeding lines. Twenty-three ISSR primers amplified a total of 267 loci, of which 224 (83.9%) were polymorphic among the genotypes. The percentage of polymorphic loci (PPL) for primers ranged from 54% (UBC808) to 100% (441, A12 and UBC820). The mean of expected heterozygosity (He) for the primers varied from 0.26 (UBC808, UBC827 and A14) to 0.48 (A12, 441). The PPL, He, Shannon’s information index (I), and number of effective alleles (Ne) for breeding lines were slightly higher than those of cultivars. The Dice similarity coefficient for the germplasm ranged from 0.76 (between two breeding lines) to 0.91 (between two breeding lines). The dendrogram grouped samples in four main clusters; most cultivars were placed into the same cluster close to each other with regard to their geographic origin. The genotypes in different groups might be used as potential parents in bread wheat breeding programs. Also, a high level of genetic similarity detected in this collection may demonstrate the narrow genetic base of Iranian wheat germplasm.

Leaf rust resistance gene Lr1, isolated from bread wheat (Triticum aestivum L.) is a member of the large psr567 gene family

2007 ◽  
Vol 65 (1-2) ◽  
pp. 93-106 ◽  
Author(s):  
Sylvie Cloutier ◽  
Brent D. McCallum ◽  
Caroline Loutre ◽  
Travis W. Banks ◽  
Thomas Wicker ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Gene Family ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Bread Wheat ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Triticum Aestivum L ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene
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