Isobaric tags for relative and absolute quantitation-based quantitative proteomics analysis provides novel insights into the mechanism of cross-incompatibility between tree peony and herbaceous peony

2019 ◽  
Vol 46 (5) ◽  
pp. 417 ◽  
Author(s):  
Dan He ◽  
Xue-Yuan Lou ◽  
Song-Lin He ◽  
Ya-Kai Lei ◽  
Bo-Va Lv ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Pollen Tube ◽  
Molecular Mechanisms ◽  
Interspecific Hybridisation ◽  
Tree Peony ◽  
Absolute Quantitation ◽  
Herbaceous Peony ◽  
Isobaric Tags ◽  
Shock Proteins

Interspecific hybridisation is the main method for improvement and breeding of tree peony (Paeonia ostii T.Hong & J.X.Zhang), but cross-incompatibility as the major factor restricting the rapid development of interspecific hybridisation. To better understand the molecular mechanisms involved in cross-incompatibility between tree peony (Paeonia ostii cv. Fengdanbai) and herbaceous peony (Paeonia lactiflora Pall. cv. Fenyunu), a quantitative proteomic analysis using isobaric tags for relative and absolute quantitation (iTRAQ) technology was performed on the stigma 24h after pollination. Of the 2900 proteins whose levels were quantitated, 685 proteins were differentially expressed in the stigma after hybrid pollination, in contrast to self-pollination. Functional annotation analysis showed that dysregulated proteins involved in RNA degradation, the Ca signalling pathway, the phosphatidylinositol signalling system and the mitogen-activated protein kinase (MAPK) signalling pathway may have made contributions to cross-incompatibility. The downregulated expression of enolase, DnaK (Heat Shock Proteins, HSP70), GroEL (Heat Shock Proteins, HSP60), calmodulin and glyoxalase I, and the upregulated expression of adenine nucleotide translocator indicated that the energy synthesis required by pollen tube growth, the signal pathway and the metabolic pathway related to the growth polarity of the pollen tube were blocked after hybrid pollination. Eight genes were selected to confirm their expression by quantitative real-time PCR. Compared with the STRING database, a protein–protein interaction network of the chosen proteins was constructed. These results provide fundamental and important information for research into the molecular mechanisms of cross-incompatibility in peony and should facilitate interspecific hybridisation in agricultural practice.

scholarly journals Proteomic Analysis Identifies Markers of Exposure to Cadmium Sulphide Quantum Dots (CdS QDs)

Nanomaterials ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1214
Author(s):  
Valentina Gallo ◽  
Vaibhav Srivastava ◽  
Vincent Bulone ◽  
Andrea Zappettini ◽  
Marco Villani ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Time Course ◽  
Cadmium Sulphide ◽  
Atp Synthesis ◽  
Absolute Quantitation ◽  
Cds Qds ◽  
Isobaric Tags ◽  
Shock Proteins

The use of cadmium sulphide quantum dot (CdS QD)-enabled products has become increasingly widespread. The prospect of their release in the environment is raising concerns. Here we have used the yeast model Saccharomyces cerevisiae to determine the potential impact of CdS QD nanoparticles on living organisms. Proteomic analyses and cell viability assays performed after 9 h exposure revealed expression of proteins involved in oxidative stress and reduced lethality, respectively, whereas oxidative stress declined, and lethality increased after 24 h incubation in the presence of CdS QDs. Quantitative proteomics using the iTRAQ approach (isobaric tags for relative and absolute quantitation) revealed that key proteins involved in essential biological pathways were differentially regulated over the time course of the experiment. At 9 h, most of the glycolytic functions increased, and the abundance of the number of heat shock proteins increased. This contrasts with the situation at 24 h where glycolytic functions, some heat shock proteins as well as oxidative phosphorylation and ATP synthesis were down-regulated. It can be concluded from our data that cell exposure to CdS QDs provokes a metabolic shift from respiration to fermentation, comparable to the situation reported in some cancer cell lines.

Induced Expression of the Heat Shock Protein Genes uspA and grpE during Starvation at Low Temperatures and Their Influence on Thermal Resistance of Escherichia coli O157:H7

2003 ◽  
Vol 66 (11) ◽  
pp. 2045-2050 ◽  
Author(s):  
YI ZHANG ◽  
MANSEL W. GRIFFITHS
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Stress Responses ◽  
Thermal Tolerance ◽  
Molecular Mechanisms ◽  
Fluorescent Protein ◽  
Bacterial Cells ◽  
Induced Expression ◽  
Green Fluorescent ◽  
Shock Proteins

Heat shock proteins play an important role in protecting bacterial cells against several stresses, including starvation. In this study, the promoters for two genes encoding heat shock proteins involved in many stress responses, UspA and GrpE, were fused with the green fluorescent protein (gfp) gene. Thus, the expression of the two genes could be quantified by measuring the fluorescence emitted by the cells under different environmental conditions. The heat resistance levels of starved and nonstarved cells during storage at 5, 10, and 37°C were compared with the levels of expression of the uspA and grpE genes. D52-values (times required for decimal reductions in count at 52°C) increased by 11.5, 14.6, and 18.5 min when cells were starved for 3 h at 37°C, for 24 h at 10°C, and for 2 days at 5°C, respectively. In all cases, these increases were significant (P < 0.01), indicating that the stress imposed by starvation altered the ability of E. coli O157:H7 to survive subsequent heat treatments. Thermal tolerance was correlative with the induction of UspA and GrpE. At 5°C, the change in the thermal tolerance of the pathogen was positively linked to the induced expression of the grpE gene but negatively related to the expression of the uspA gene. The results obtained in this study indicate that UspA plays an important role in starvation-induced thermal tolerance at 37°C but that GrpE may be more involved in regulating this response at lower temperatures. An improvement in our understanding of the molecular mechanisms involved in these cross-protection responses may make it possible to devise strategies to limit their effects.

scholarly journals Molecular mechanisms of heat shock proteins (HSPs) involved in neoplasm development

2019 ◽  
Vol 34 (2) ◽  
pp. 455-474
Author(s):  
Rafael Guerrero-Rojas ◽  
Carlos Guerrero-Fonsecaz
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Molecular Mechanisms ◽  
Shock Proteins

scholarly journals Type 2 diabetes and metabolic syndrome: identification of the molecular mechanisms, key signaling pathways and transcription factors aimed to reveal new therapeutical targets

2018 ◽  
Vol 21 (5) ◽  
pp. 364-375 ◽  
Author(s):  
Ivan I. Dedov ◽  
Vsevolod A. Tkachuk ◽  
Nikolai B. Gusev ◽  
Vladimir P. Shirinsky ◽  
Aleksandr V. Vorotnikov ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Heat Shock ◽  
Endothelial Dysfunction ◽  
Heat Shock Proteins ◽  
Molecular Mechanisms ◽  
Small Heat Shock Proteins ◽  
Target Cells ◽  
Beige Fat ◽  
Shock Proteins

Type 2 diabetes mellitus (T2DM) is a socially important disease with only symptomatic therapy developed due to lack of knowledge about its pathogenesis and underlying mechanism. Insulin resistance (IR) is the first link of T2DM pathogenesis and results in decrease of ability of insulin to stimulate glucose uptake by target cells. Development of IR involves genetic predisposition, excessive nutrition, stress, obesity or chronic inflammation due to disruption of insulin signaling within cells. Molecular mechanisms and markers of IR are characterized rather poorly, which prevents early diagnosis and creation of preventive therapy. Euglycemic clamp test is still a golden standard for IR diagnosis in clinic. Hyperglycemia is a distant consequence of IR in which damaging effect of oxidative and carbonyl stress is realized and diagnosis of T2DM is stipulated. Molecular chaperones and small heat-shock proteins have a protective effect at the early stages of T2DM pathogenesis, preventing development of reticulum stress and apoptosis. Endothelial dysfunction is related to T2DM and its cardiovascular complications, however, it is unknown on which stage of pathogenesis these changes occur and what are their molecular inductors. Finally, transcriptional activity and adipogenic differentiation play an important role in formation of new fat depots from predecessor cells and activation of brown and beige fat demonstrating hypolipidemic and hypoglycemic properties. The aim of this study was investigation of pathophysiological mechanisms of development of IR and endothelial dysfunction, role of transcription factor Prep1 and small heat shock proteins, evaluation of novel methods of diagnostics of IR and therapeutic potential of brown and beige fat, determination of biotargets for new antidiabetic drugs.

Molecular Mechanisms of Heat Shock Proteins for Sustainable Plant Growth and Production

2021 ◽  
pp. 141-169
Author(s):  
Maria Kidwai ◽  
Puja Singh ◽  
Prasanna Dutta ◽  
Khushboo Chawda ◽  
Debasis Chakrabarty
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Plant Growth ◽  
Molecular Mechanisms ◽  
Shock Proteins

Genetic regulation during heat shock and function of heat-shock proteins: a review

1983 ◽  
Vol 61 (6) ◽  
pp. 387-394 ◽  
Author(s):  
R. M. Tanguay
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Posttranscriptional Regulation ◽  
Translational Regulation ◽  
Cellular Localization ◽  
Molecular Mechanisms ◽  
Genetic Regulation ◽  
Protective Function ◽  
Shock Proteins ◽  
And Function

The induction by thermal stress of certain specific genes (heat-shock genes) first described in Drosophila has recently been observed in a wide variety of unicellular and multicellular organisms, emphasizing the basic importance of this ubiquitous response. Recent data dealing with the molecular mechanisms involved in the intensive transcriptional and posttranscriptional regulation during heat shock is reviewed with emphasis on the induction of the response and the putative function of the heat-shock proteins. A model showing the various interactions of cellular regulatory mechanisms operating in the heat-shocked cell is presented. While the list of agents or treatments inducing heat-shock proteins (hsp's) in various organisms is increasing, the identification of a hypothetical common inducing factor is elusive. The recently described reorganization of some cytoskeletal elements upon heat shock is discussed both in terms of its potential involvement in transcriptional and (or) translational regulation and of its putative relation with the cellular localization of the hsp's. Studies on the cellular localization of hsp's in various organisms do not show a clear uniform pattern which could help in elucidating the function of hsp's. On the other hand, studies on the thermal resistance of various cells types show a strong correlation between the induction of hsp's and the development of transitory thermotolerance. Such a protective function for hsp's can probably be extended to other types of cellular aggression.

scholarly journals Combination of transcriptome sequencing and iTRAQ proteome reveals the molecular mechanisms determining petal shape in herbaceous peony (Paeonia lactiflora Pall.)

2018 ◽  
Vol 38 (6) ◽  
Author(s):  
Yanqing Wu ◽  
Yuhan Tang ◽  
Yu Jiang ◽  
Daqiu Zhao ◽  
Jiali Shang ◽  
...  
Keyword(s):  
Transcriptome Sequencing ◽  
Molecular Mechanisms ◽  
Differentially Expressed ◽  
Paeonia Lactiflora ◽  
Absolute Quantitation ◽  
Herbaceous Peony ◽  
Polymerase Chain ◽  
Petal Shape ◽  
Isobaric Tags ◽  
Rapid Amplification

The molecular mechanisms controlling petal shape in a herbaceous peony, Paeonia lactiflora Pall., a popular high-grade cut flower worldwide, remain unclear. Here, we selected inner and outer petals from P. lactiflora ‘ZiFengyu’ with an anemone type as the study object. Using transcriptome sequencing and isobaric tags for relative and absolute quantitation proteome, 979 differentially expressed genes and 266 differentially expressed proteins were detected within the inner and outer petals. Of these, the present study identified a key gene APETALA2 that regulates flower shape development. In addition, we obtained a 1935 bp full-length cDNA sequence of APETALA2 by rapid amplification of cDNA ends amplification. Through further validation using quantitative real-time polymerase chain reaction and Western blot analysis, APETALA2 showed a markedly higher expression in outer than that in inner petals. Therefore, the present study indicates that the increased expression of APETALA2 contributes to the formation of petals in P. lactiflora.

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