Observations on the Aleurone Layer. II. Fluorescence Microscopy of the Aleurone-Sub-Aleurone Junction With Emphasis on Possible β-1,3-Glucan Deposits in Barley
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The structural organization of the cell walls at the aleurone-sub-aleurone junction has been examined by fluorescence microscopy in ungerminated seeds of two varieties of Hordeum vulgare L. (cultivars Himalaya and Vanier). The sub-aleurone cell walls that are immediately adjacent to the aleurone layer are considerably thicker than the remainder of endosperm walls and contain extensive deposits of aniline blue-positive material. The latter was not significantly affected by periodate oxidation and was removed from tissue sections only by β-1,3-glucanases or hot dimethyl sulphoxide. These deposits may represent the primary substrate for endo-β-1,3-glucanases secreted by the aleurone layer during germination.
2003 ◽
Vol 35
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pp. 1261-1270
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2019 ◽
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1994 ◽
Vol 5
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pp. 385-396
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2018 ◽
Vol 50
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pp. 139-144
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2015 ◽
Vol 57
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pp. 55-66
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1992 ◽
Vol 50
(1)
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pp. 844-845
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2016 ◽
Vol 51
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pp. 335-342
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