Effects of oxygen concentration on the metabolism of

1989 ◽  
Vol 1 (2) ◽  
pp. 99 ◽  
Author(s):  
NK Khurana ◽  
RG Wales
Keyword(s):  
Carbon Dioxide ◽  
Oxygen Concentration ◽  
Glycogen Content ◽  
Carbon Dioxide Production ◽  
Morphological Development ◽  
Low Oxygen ◽  
Beneficial Effects ◽  
Low Oxygen Concentration ◽  
Oxygen Concentrations

The utilization of the acid-soluble glycogen pool in pulse-labelled embryos was significantly enhanced during 24- and 48-h chase culture under low oxygen concentrations of 5, 2.5 and 1%. The lower the oxygen tension the greater was the turnover in the pool. The morphological development of embryos was equally as good at very low oxygen concentrations as when embryos were cultured in 5-20% oxygen. Reduction in oxygen concentration enhanced the oxidative utilization of substrate, as measured by rate of carbon dioxide production. The present study could provide an explanation for the discrepancy in glycogen content between mouse blastocysts developing in utero and in vitro and for the reported beneficial effects of low oxygen concentration during development of embryos in culture.

Effects of oxygen concentrations on developmental competence and transcriptomic profile of yak oocytes

Zygote ◽  
2020 ◽  
Vol 28 (6) ◽  
pp. 459-469
Author(s):  
Ruizhe Li ◽  
Yuzhu Luo ◽  
Jingtao Xu ◽  
Yonggang Sun ◽  
Zhijie Ma ◽  
...  
Keyword(s):  
Oxygen Concentration ◽  
Enrichment Analysis ◽  
Oocyte Quality ◽  
Signalling Pathway ◽  
Developmental Competence ◽  
Low Oxygen ◽  
Transcriptomic Profile ◽  
Low Oxygen Concentration ◽  
Oxygen Concentrations

SummaryOxygen concentration influences oocyte quality and subsequent embryo development, but it remains unclear whether oxygen concentrations affect the developmental competence and transcriptomic profile of yak oocytes. In this study, we investigated the effects of different oxygen concentrations (5% versus 20%) on the developmental competence, reactive oxygen species (ROS) levels, glutathione (GSH) content, and transcriptomic profile of yak oocytes. The results showed that a low oxygen concentration significantly increased the maturation rate of yak oocytes (81.2 ± 2.2% vs 75.9 ± 1.3%) and the blastocyst quality of yak in vitro fertilized embryos. Analysis of ROS and GSH showed that a low oxygen concentration reduced ROS levels and increased the content of GSH (75.05 ± 7.1 ng/oocyte vs 50.63 ± 5.6 ng/oocyte). Furthermore, transcriptomic analysis identified 120 differentially expressed genes (DEGs) between the two groups of oocytes. Gene enrichment analysis of the DEGs indicated multiple cellular processes, including oxidative phosphorylation, transcription regulation, mitochondrial regulation, oestrogen signalling pathway, HIF-1 signalling pathway, TNF signalling pathway, were involved in the response to oxygen concentration alterations. Taken together, these results indicated that a low oxygen concentration improved the developmental competence of yak oocytes.

scholarly journals High-Sustained Concentrations of Organisms at Very low Oxygen Concentration Indicated by Acoustic Profiles in the Oxygen Deficit Region Off Peru

2021 ◽  
Vol 8 ◽  
Author(s):  
Aurelien Paulmier ◽  
Gerard Eldin ◽  
José Ochoa ◽  
Boris Dewitte ◽  
Joël Sudre ◽  
...  
Keyword(s):  
Oxygen Concentration ◽  
Acoustic Doppler Current Profiler ◽  
Vertical Gradient ◽  
Time Of Day ◽  
Oxygen Deficit ◽  
Low Oxygen ◽  
Current Profiler ◽  
Organic Particles ◽  
Low Oxygen Concentration ◽  
Oxygen Concentrations

The oxygen deficient mesopelagic layer (ODL) off Peru has concentrations below 5 μmol O2 kg–1 and is delimited by a shallow upper oxycline with strong vertical gradient and a more gradual lower oxycline (lOx). Some regions show a narrow band of slightly increased oxygen concentrations within the ODL, an intermediate oxygen layer (iO2). CTD, oxygen and lowered Acoustic Doppler Current Profiler (LADCP, 300 kHz) profiles were taken on the shelf edge and outside down to mostly 2000 m. We evaluate here the acoustic volume backscatter strength of the LADCP signal representing organisms of about 5 mm size. Dominant features of the backscatter profiles were a minimum backscatter strength within the ODL, and just below the lOx a marked backscatter increase reaching a maximum at less than 3.0 μmol O2 kg–1. Below this maximum, the acoustic backscatter strength gradually decreased down to 1000 m below the lOx. The backscatter strength also increased at the iO2 in parallel to the oxygen concentration perturbations marking the iO2. These stable backscatter features were independent of the time of day and the organisms represented by the backscatter had to be adapted to live in this microaerobic environment. During daylight, these stable structures were overlapped by migrating backscatter peaks. Outstanding features of the stable backscatter were that at very low oxygen concentrations, the volume backscatter was linearly related to the oxygen concentration, reaching half peak maximum at less than 2.0 μmol O2 kg–1 below the lOx, and the depth-integrated backscatter of the peak below the lOx was higher than the integral above the Ox. Both features suggest that sufficient organic material produced at the surface reaches to below the ODL to sustain the major fraction of the volume backscatter-producing organisms in the water column. These organisms are adapted to the microaerobic environment so they can position themselves close to the lower oxycline to take advantage of the organic particles sinking out of the ODL.

MAINTENANCE OF ASCARIS LUMBRICOIDES IN VITRO: A BIOCHEMICAL AND STATISTICAL APPROACH

1962 ◽  
Vol 40 (6) ◽  
pp. 991-1011 ◽  
Author(s):  
R. P. Harpur
Keyword(s):  
Carbon Dioxide ◽  
Amino Acids ◽  
Carbon Dioxide Production ◽  
Gas Production ◽  
Ion Concentration ◽  
Ammonium Ions ◽  
High Potassium ◽  
Beneficial Effects ◽  
The Media

The anaerobic carbon dioxide production of minced ascaris muscle decreases markedly when worms are kept in vitro for 3 days. Using this gas production as an index, and a factorial design, the effects of nitrogen, oxygen, carbon dioxide, antibiotics, vitamins, amino acids, glucose, potassium, and ammonium ions were investigated. For this study the worms were kept in media which were changed every 3 hours and the gaseous phases were maintained by bubbling gas continuously through the media. Under these conditions 20% oxygen was extremely toxic but carbon dioxide (5%) provided some relief from this effect. Even 5% oxygen caused more decrease in the index than did nitrogen alone, but this was not true when a mixture of amino acids and glucose was present. Ammonium ions had a beneficial effect in nitrogen but a detrimental effect in the presence of 20% oxygen. A high potassium ion concentration (24 mM) was detrimental in the absence of carbon dioxide but appeared to enhance the beneficial effects of carbon dioxide.

Effect of low oxygen concentration on activation of inflammation by Helicobacter pylori

2021 ◽  
Vol 560 ◽  
pp. 179-185
Author(s):  
Adiza Abass ◽  
Tokuju Okano ◽  
Kotchakorn Boonyaleka ◽  
Ryo Kinoshita-Daitoku ◽  
Shoji Yamaoka ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Oxygen Concentration ◽  
Low Oxygen ◽  
Low Oxygen Concentration

Thermogravimetric and infrared spectral analysis of candle coal pyrolysis under low-oxygen concentration

2021 ◽  
Vol 696 ◽  
pp. 178840
Author(s):  
Liangzhou Chen ◽  
Xuyao Qi ◽  
Jian Yang ◽  
Haihui Xin
Keyword(s):  
Spectral Analysis ◽  
Oxygen Concentration ◽  
Coal Pyrolysis ◽  
Low Oxygen ◽  
Infrared Spectral ◽  
Low Oxygen Concentration

The Constant Low Oxygen Concentration in All the Target Cells for Mouse Tail Radionecrosis

1982 ◽  
Vol 92 (1) ◽  
pp. 172 ◽  
Author(s):  
J. H. Hendry ◽  
J. V. Moore ◽  
B. W. Hodgson ◽  
J. P. Keene
Keyword(s):  
Oxygen Concentration ◽  
Target Cells ◽  
Low Oxygen ◽  
Low Oxygen Concentration

Beneficial effects of carbon dioxide on development of gerbera and rose plantlets grown in vitro

1990 ◽  
Vol 44 (3-4) ◽  
pp. 341-345 ◽  
Author(s):  
Ernst J. Woltering
Keyword(s):  
Carbon Dioxide ◽  
Beneficial Effects
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