Twenty microsatellite loci for population and conservation genetic studies of the wedge-tailed eagle (Aquila audax)

2014 ◽  
Vol 62 (3) ◽  
pp. 235 ◽  
Author(s):  
J. J. Austin ◽  
L. Olivier ◽  
D. Nankervis ◽  
W. E. Brown ◽  
M. G. Gardner ◽  
...  
Keyword(s):  
Population Structure ◽  
Linkage Disequilibrium ◽  
Gene Flow ◽  
Conservation Genetics ◽  
Microsatellite Loci ◽  
Population Differentiation ◽  
Genetic Studies ◽  
Genotype Frequencies ◽  
Conservation Genetic ◽  
Hardy Weinberg Equilibrium

Twenty di- to pentanucleotide microsatellites are reported for the wedge-tailed eagle (Aquila audax), a large raptor from Australia, Tasmania, and New Guinea. These loci were tested for variation among 49 individuals. All loci are polymorphic with 2–14 alleles per locus, and observed heterozygosities ranged between 0.021 and 0.898. Genotype frequencies for all loci did not differ significantly from Hardy–Weinberg equilibrium and there was no evidence of linkage disequilibrium. These markers will be used to assess population structure and conservation genetics of this species, focusing on population differentiation and gene flow between Tasmanian and mainland populations and conservation genetics of the endangered Tasmanian population.

scholarly journals Development and characterisation of polymorphic microsatellite markers in the pearl oyster Pteria penguin (Roding, 1798)

2016 ◽  
Vol 63 (4) ◽  
Author(s):  
Nan Zhang ◽  
Zhenhua Ma ◽  
Dianchang Zhang ◽  
Huayang Guo ◽  
Lin Lin ◽  
...  
Keyword(s):  
Linkage Disequilibrium ◽  
Microsatellite Markers ◽  
Pearl Oyster ◽  
Significant Linkage Disequilibrium ◽  
Genetic Studies ◽  
Pteria Penguin ◽  
Conservation Genetic ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite ◽  
Significant Linkage

We developed and characterised 12 polymorphic microsatellite loci for Pteria penguin (Roding, 1798), an important pearl oyster species for pearl production in China. The number of observed alleles per locus ranged from 5 to 26 within 46 individuals. The observed and expected heterozygosities per locus ranged from 0.2 to 0.925 and 0.707 to 0.945, respectively. The polymorphism information content values per locus ranged from 0.656 to 0.929. Ten loci were in accordance with Hardy-Weinberg equilibrium and no significant linkage disequilibrium was detected between loci. These new microsatellite markers will be useful for population and conservation genetic studies of P. penguin.

Fifteen microsatellite loci for use in non-invasive sampling studies of the antilopine wallaroo (Macropus antilopinus)

2013 ◽  
Vol 61 (5) ◽  
pp. 399
Author(s):  
Jessica J. Wadley ◽  
Jeremy J. Austin ◽  
Michael G. Gardner ◽  
Damien A. Fordham
Keyword(s):  
Population Structure ◽  
Microsatellite Loci ◽  
Sampling Studies ◽  
Non Invasive ◽  
Genotype Frequencies ◽  
Eastern Australia ◽  
North Eastern ◽  
Hardy Weinberg Equilibrium ◽  
Two Populations

A set of 15 microsatellite loci was optimised for multilocus genotyping of non-invasively collected samples of Macropus antilopinus (antilopine wallaroo). Primers were combined in three PCR multiplexes in order to increase the quality of genotypes from scat samples and to allow for replication. In a screen of 104 scat samples from two populations in north-eastern Australia, three loci were found to be monomorphic while the remaining 12 loci had 2–10 alleles. Genotype frequencies for all 12 microsatellite loci from the two populations did not differ significantly from Hardy–Weinberg equilibrium, and there was no evidence of linkage disequilibrium. These informative markers are specifically designed for non-invasive samples and will be used to assess population structure and conservation genetics of this species in the future.

Identification of seventeen microsatellite loci for conservation genetic studies of the endemic wrasse Coris bulbifrons

2012 ◽  
Vol 5 (2) ◽  
pp. 363-366 ◽  
Author(s):  
Martin H. van der Meer ◽  
Michael G. Gardner ◽  
Michael L. Berumen ◽  
Jean-Paul A. Hobbs ◽  
Lynne van Herwerden
Keyword(s):  
Microsatellite Loci ◽  
Genetic Studies ◽  
Conservation Genetic

Genetic Variation at the Malic Enzyme-2 Locus and Age at Maturity in Sea-run Atlantic Salmon (Salmo salar)

1990 ◽  
Vol 47 (9) ◽  
pp. 1672-1677 ◽  
Author(s):  
W. C. Jordan ◽  
A. F. Youngson ◽  
J. H. Webb
Keyword(s):  
Population Structure ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
Malic Enzyme ◽  
Commercial Fishery ◽  
Age At Maturity ◽  
Frequency Distributions ◽  
Genotype Frequencies ◽  
Hardy Weinberg Equilibrium ◽  
Selective Influence

Variation in the diallelic polymorphism at the malic enzyme-2 locus (Me-2) in Atlantic salmon is used extensively in descriptions of the population structure of this species. Genotypes at the Me-2 locus were examined in adult Atlantic salmon captured at the Girnock Burn, a tributary of the Aberdeenshire Dee, over 3 yr (1986–88), and in salmon caught in the commercial fishery in the estuary of the River Tay in 2 yr (1987–88). Initial observations on the Girnock Burn data suggested that Me-2 genotype frequency distributions differed between one-sea-winter fish (grilse) and multi-sea-winter fish (salmon) groups from the same hatch-year class. Data from the River Tay supported hypotheses constructed on the basis of the Girnock Burn data. Genotype frequencies in some grilse groups at both sites differed significantly from those expected under Hardy–Weinberg equilibrium, while those in salmon groups did not. The frequency of heterozygotes was generally significantly higher in grilse than in salmon from the same hatch-year class. The data suggest a variable association between Me-2 genotype and age at maturity, and a selective influence on the Me-2 locus. The implications of selective influences on this locus for estimates of gene flow among Atlantic salmon populations are discussed.

Contrasting population structures of three Pristis sawfishes with different patterns of habitat use

2017 ◽  
Vol 68 (3) ◽  
pp. 452 ◽  
Author(s):  
N. M. Phillips ◽  
J. A. Chaplin ◽  
S. C. Peverell ◽  
D. L. Morgan
Keyword(s):  
Population Structure ◽  
Gene Flow ◽  
Habitat Use ◽  
Microsatellite Loci ◽  
Spatial Scales ◽  
Marine Waters ◽  
Male And Female ◽  
Entire Life ◽  
Population Structures ◽  
Using Data

This research demonstrates how population structure differs in elasmobranchs with different patterns of habitat use. Population structure was assessed using data at microsatellite loci in three species of Pristis sawfishes in northern Australian waters. Statistically significant population structure was found in each of P. clavata (FST = 0.021, F′ST = 0.151, P < 0.001) and P. zijsron (FST = 0.026, F′ST = 0.130, P < 0.001), which spend their entire life in marine waters. In contrast, there was no evidence of significant population structure in P. pristis, which uses freshwater rivers as juveniles and marine waters as adults (FST = 0.004, F′ST = 0.029, P = 0.210). When combined with the results of mtDNA analyses from a previous study, the results suggested that dispersal in P. pristis is male-biased, whereas both male and female gene flow are restricted at large spatial scales in each of P. clavata and P. zijsron in Australian waters. The present study has provided the first evidence of sex-biased dispersal in a sawfish.

Isolation and characterisation of 16 polymorphic microsatellite loci for bight redfish, Centroberyx gerrardi (Actinopterygii : Berycidae), and cross-amplification in two other Centroberyx species

2015 ◽  
Vol 63 (4) ◽  
pp. 275
Author(s):  
Andrea Bertram ◽  
P. Joana Dias ◽  
Sherralee Lukehurst ◽  
W. Jason Kennington ◽  
David Fairclough ◽  
...  
Keyword(s):  
Population Structure ◽  
Linkage Disequilibrium ◽  
Spatial Scales ◽  
Weinberg Equilibrium ◽  
Cross Amplification ◽  
Stock Assessments ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite ◽  
Population Genetic Analyses ◽  
Upper Slope

Bight redfish, Centroberyx gerrardi, is a demersal teleost endemic to continental shelf and upper slope waters of southern Australia. Throughout most of its range, C. gerrardi is targeted by a number of separately managed commercial and recreational fisheries across several jurisdictions. However, it is currently unknown whether stock assessments and management for this shared resource are being conducted at appropriate spatial scales, thereby requiring knowledge of population structure and connectivity. To investigate population structure and connectivity, we developed 16 new polymorphic microsatellite markers using 454 shotgun sequencing. Two to 15 alleles per locus were detected. There was no evidence of linkage disequilibrium between pairs of loci and all loci except one were in Hardy–Weinberg equilibrium. Cross-amplification trials in the congeneric C. australis and C. lineatus revealed that 11 and 16 loci are potentially useful, respectively. However, deviations from Hardy–Weinberg equilibrium and linkage disequilibrium between pairs of loci were detected at several of the 16 markers for C. australis, and therefore the number of markers useful for population genetic analyses with C. lineatus is likely considerably lower than 11.

Characterization of 22 microsatellite loci for conservation genetic studies of an endemic anemonefish, Amphiprion latezonatus

2014 ◽  
Vol 7 (1) ◽  
pp. 95-97 ◽  
Author(s):  
Rosemary Steinberg ◽  
Martin van der Meer ◽  
Jean Paul Hobbs ◽  
Michael L. Berumen ◽  
Lynne van Herwerden
Keyword(s):  
Microsatellite Loci ◽  
Genetic Studies ◽  
Conservation Genetic

scholarly journals Range-Wide and Regional Patterns of Population Structure and Genetic Diversity in the Gopher Tortoise

2017 ◽  
Vol 8 (2) ◽  
pp. 497-512 ◽  
Author(s):  
D. Gaillard ◽  
J.R. Ennen ◽  
B.R. Kreiser ◽  
C.P. Qualls ◽  
S.C. Sweat ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Gene Flow ◽  
Microsatellite Loci ◽  
Spatial Scales ◽  
Demographic History ◽  
Genetic Admixture ◽  
Gopher Tortoise ◽  
Regional Patterns ◽  
Genetic Groups

Abstract The gopher tortoise (Gopherus polyphemus) has experienced dramatic population declines throughout its distribution in the southeastern United States and is federally listed as threatened in the area west of the Tombigbee and Mobile rivers. While there is molecular support for recognizing the listed portion of the range as genetically distinct, other research has suggested that additional population structure exists at both range-wide and regional scales. In this study, we sought to comprehensively define genetic population structure at both spatial scales by doubling the data available in terms of the number of sampling sites, individuals, and microsatellite loci compared to previously published work. We also compared patterns of genetic diversity, gene flow, and demographic history across the range. We collected 933 individuals from 47 sampling sites across the range and genotyped them for 20 microsatellite loci. Our range-wide analyses supported the recognition of five genetic groups (or regions) delineated by the Tombigbee and Mobile rivers, Apalachicola and Chattahoochee rivers, and the transitional areas between several physiographic province sections of the Coastal Plains (i.e., Eastern Gulf, Sea Island, and Floridian). We found genetic admixture at sampling sites along the boundaries of these genetically defined groups. We detected some degree of additional genetic structure within each of the five regions. Notably, within the range listed as threatened under the Endangered Species Act, we found some support for two additional genetic groups loosely delineated by the Pascagoula and Chickasawhay rivers, and we detected four more genetic groups within the Florida region that seemed to reflect the influence of the local physiography. Additionally, our range-wide analysis found the periphery of the range had lower levels of genetic diversity relative to the core. We suggest that the five main genetic groups delineated in our study warrant recognition as management units in terms of conservation planning. Intraregional population structure also points to the potential importance of other barriers to gene flow at finer spatial scales, although additional work is needed to better delineate these genetic groups.

scholarly journals Isolation and Characterization of Twenty Polymorphic Microsatellite Loci for Moringa oleifera (Moringaceae)

HortScience ◽  
2010 ◽  
Vol 45 (4) ◽  
pp. 690-692 ◽  
Author(s):  
Jiang-Chong Wu ◽  
Jing Yang ◽  
Zhi-Jian Gu ◽  
Yan-Ping Zhang
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Population Genetic ◽  
Moringa Oleifera ◽  
Microsatellite Primers ◽  
Genetic Studies ◽  
Isolation And Characterization ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite

By using a modified biotin-streptavidin capturing method, a total of 20 polymorphic microsatellite markers were developed from Moringa oleifera Lam. (Moringaceae), a useful multipurpose tree. Twenty-four domesticated individuals, with germplasms of India and Myanmar, were used to screen polymorphism of these 20 microsatellite markers. The number of alleles per locus ranged from two to six. The expected and observed heterozygosity varied from 0.3608 to 0.7606 and from 0.0000 to 0.8750, respectively. Seven loci were significantly deviated from Hardy-Weinberg equilibrium. The availability of these microsatellite primers would provide a powerful tool for aspects of detailed population genetic studies of M. oleifera.

scholarly journals Microsatellite Loci Transferability in Chestnut

2008 ◽  
Vol 133 (5) ◽  
pp. 692-700 ◽  
Author(s):  
Ying Wang ◽  
Ming Kang ◽  
Hongwen Huang
Keyword(s):  
Microsatellite Loci ◽  
Castanea Sativa ◽  
Breeding Programs ◽  
Genetic Studies ◽  
European Chestnut ◽  
Expected Heterozygosity ◽  
Conservation Genetic

Cross-species amplification of 55 microsatellite loci developed in european chestnut (Castanea sativa Mill.) and japanese chestnut (C. crenata Sieb & Zucc.) was tested in three chestnut species from China [C. mollissima Blume, C. seguinii Dode, and C. henryi (Skan.) Rehder & Wilson]. Among all the tested loci, 47 (85.5%), 47 (85.5%), and 44 (80%) were successfully amplified in each of the three Chinese species, respectively. All microsatellite loci tested from C. crenata successfully amplified in the Chinese species, while only 80.5%, 80.5%, and 73.2% of the loci originating from C. sativa amplified in the three Chinese species. The level of polymorphism and mean number of alleles was 58.2% and 4.12 for C. mollissima, 60% and 4.64 for C. seguinii, and 60% and 4.76 for C. henryi, with mean observed heterozygosity ranging from 0.440 to 0.549 and mean expected heterozygosity ranging from 0.506 to 0.615. Transferability of Castanea Mill. microsatellites provides a powerful tool for chestnut breeding programs and conservation genetic studies of Castanea species.

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