scholarly journals Mechanism of oligonucleotide uptake by cells: involvement of specific receptors?

1989 ◽  
Vol 86 (17) ◽  
pp. 6454-6458 ◽  
Author(s):  
L A Yakubov ◽  
E A Deeva ◽  
V F Zarytova ◽  
E M Ivanova ◽  
A S Ryte ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Mouse Fibroblast ◽  
Carcinoma Cells ◽  
Dna And Rna ◽  
Chondroitin Sulfates ◽  
Double Stranded Dna ◽  
L929 Mouse Fibroblast ◽  
Specific Receptors ◽  
L929 Mouse ◽  
Oligonucleotide Derivatives

We have investigated the interaction of oligonucleotides and their alkylating derivatives with mammalian cells. In experiments with L929 mouse fibroblast and Krebs 2 ascites carcinoma cells, it was found that cellular uptake of oligodeoxynucleotide derivatives is achieved by an endocytosis mechanism. Uptake is considerably more efficient at low oligomer concentration (less than 1 microM), because at this concentration a significant percentage of the total oligomer pool is absorbed on the cell surface and internalized by a more efficient absorptive endocytosis process. Two modified proteins were detected in mouse fibroblasts that were treated with the alkylating oligonucleotide derivatives. The binding of the oligomers to the proteins is inhibited by other oligodeoxynucleotides, single- and double-stranded DNA, and RNA. The polyanions heparin and chondroitin sulfates A and B do not inhibit binding. These observations suggest the involvement of specific receptor proteins in binding of oligomers to mammalian cells.

A nano chitosan membrane barrier prepared via Nanospider technology with non-toxic solvent for peritoneal adhesions’ prevention

2021 ◽  
pp. 088532822110081
Author(s):  
Shuo Zhang ◽  
Zhuoyue Xu ◽  
Xuejun Wen ◽  
Changzheng Wei
Keyword(s):  
Mouse Fibroblast ◽  
Electrospun Membrane ◽  
Adhesion Barrier ◽  
Peritoneal Adhesions ◽  
Elongation At Break ◽  
In Vivo Experiments ◽  
L929 Mouse Fibroblast ◽  
Membrane Barrier ◽  
L929 Mouse

Peritoneal adhesion is one of the most common postsurgical complications and can cause bowel obstruction, pelvic pain, and infertility. Setting up a physical barrier directly between the injured site and surrounding tissues is an effective solution for preventing this adverse situation. This study investigated a chitosan electrospun membrane (CSEM) as a potent anti-adhesion barrier, which was prepared by a needleless technology called Nanospider. Scanning electron microscopy revealed that CSEM is a laminated nanofiber with good mechanical properties. The fiber is uniform with the diameter distributing in the range of 100–120 nm. The tensile strength can reach 27.45 ± 6.30 MPa with a maximum elongation at break of 18.50 ± 1.44%, which makes it stick easily to damaged parts but not to be easily damaged by tissue friction. The growth of S. aureus on CSEM was 59.18% lower than the control at 10 h, which indicates its better antibacterial property. In addition, CSEM has good coagulant and biocompatibility characteristics. It can perform hemostatic function within 10 min and the L929 mouse fibroblast viability on it was 92.18% ± 1.08% on the seventh day. In vivo experiments indicated that CSEM significantly prevented peritoneal adhesions within four weeks after surgery with wound surface coverage. These results indicate that CSEM is a promising anti-adhesion barrier material.

scholarly journals A new series of 2,4-thiazolidinediones endowed with potent aldose reductase inhibitory activity

2021 ◽  
Vol 19 (1) ◽  
pp. 347-357
Author(s):  
Belgin Sever ◽  
Mehlika Dilek Altıntop ◽  
Yeliz Demir ◽  
Cüneyt Türkeş ◽  
Kaan Özbaş ◽  
...  
Keyword(s):  
Aldose Reductase ◽  
In Silico ◽  
Aromatic Aldehydes ◽  
Docking Studies ◽  
Mouse Fibroblast ◽  
L929 Mouse Fibroblast ◽  
Orally Bioavailable ◽  
L929 Mouse ◽  
Solvent Free Reaction

Abstract In an effort to identify potent aldose reductase (AR) inhibitors, 5-(arylidene)thiazolidine-2,4-diones (1–8), which were prepared by the solvent-free reaction of 2,4-thiazolidinedione with aromatic aldehydes in the presence of urea, were examined for their in vitro AR inhibitory activities and cytotoxicity. 5-(2-Hydroxy-3-methylbenzylidene)thiazolidine-2,4-dione (3) was the most potent AR inhibitor in this series, exerting uncompetitive inhibition with a K i value of 0.445 ± 0.013 µM. The IC50 value of compound 3 for L929 mouse fibroblast cells was determined as 8.9 ± 0.66 µM, pointing out its safety as an AR inhibitor. Molecular docking studies suggested that compound 3 exhibited good affinity to the binding site of AR (PDB ID: 4JIR). Based upon in silico absorption, distribution, metabolism, and excretion data, the compound is predicted to have favorable pharmacokinetic features. Taking into account the in silico and in vitro data, compound 3 stands out as a potential orally bioavailable AR inhibitor for the management of diabetic complications as well as nondiabetic diseases.

A Synthetic Polyanion Polymer as a Growth Promoter for L929 Mouse Fibroblast

1996 ◽  
pp. 285-286
Author(s):  
Tomohiro Sawa ◽  
Yukihisa Okumura ◽  
Jian L. Ding ◽  
Raphael M. Ottenbrite ◽  
Junzo Sunamoto
Keyword(s):  
Mouse Fibroblast ◽  
Growth Promoter ◽  
L929 Mouse Fibroblast ◽  
L929 Mouse

scholarly journals Blends of Thermoplastic Polyurethane and Polydimethylsiloxane Rubber: Assessment of Biocompatibility and Suture Holding Strength of Membranes

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Krishna Prasad Rajan ◽  
Ahmed Al-Ghamdi ◽  
Ramesh Parameswar ◽  
G. B. Nando
Keyword(s):  
Cell Proliferation ◽  
Thermoplastic Polyurethane ◽  
Mouse Fibroblast ◽  
Tissue Cell ◽  
Connective Tissue Cell ◽  
L929 Mouse Fibroblast ◽  
Subcutaneous Connective Tissue ◽  
L929 Mouse ◽  
Reactive Compatibilizer

In the present investigation, a compatibilized blend of thermoplastic polyurethane (TPU) and polydimethylsiloxane (PDMS) is prepared by using copolymer of ethylene and methyl acrylate (EMA) as a reactive compatibilizer. Detailedin vitrobiocompatibility studies were carried out for this compatibilized blend and the material was found noncytotoxic towards L929 mouse fibroblast subcutaneous connective tissue cell line. Microporosity was created on the surface of membranes prepared from the blend material by adopting the crazing mechanism. Cell proliferation and growth studies on the membranes surface showed that the microporous surface favoured ingrowth of the cells compared with a nonmicroporous surface. Suture holding strength studies indicate that the microporous membranes have enough strength to withstand the cutting and tearing forces through the suture hole. This blend material could be evaluated further to find its suitability in various implant applications.

scholarly journals Synthesis of the Macrolactone Cores of Maltepolides via a Diene–Ene Ring-Closing Metathesis Strategy

2021 ◽  
Author(s):  
Man Ki Sit ◽  
Hui Hui Cao ◽  
Yan-Dong Wu ◽  
Tsz Chun Yip ◽  
Lars Eric Bendel ◽  
...  
Keyword(s):  
Ring Opening ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Epoxide Ring ◽  
Fibroblast Cell Line ◽  
Ring Closing Metathesis ◽  
Double Bond Migration ◽  
L929 Mouse Fibroblast ◽  
Mouse Fibroblast Cell Line ◽  
L929 Mouse

Synthesis of the C19-truncated maltepolide E has been accomplished via a diene–ene RCM strategy without damage to the C11–C14 alkenyl epoxy unit. Upon release of the C17-OH group, it attacked at the C14 position with double bond migration and epoxide ring-opening to furnish the C19-truncated maltepolide A and B as proposed for the biosynthesis of maltepolides. Preliminary cytotoxicity data of the synthesized C19-truncated maltepolides against L929 mouse fibroblast cell line suggest irrelevance of the vinyl epoxide and importance of the conjugated dienyl keto unit for the observed anticancer activity.

scholarly journals In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells

2021 ◽  
Vol 25(5) (25(5)) ◽  
pp. 608-617
Author(s):  
Rosa Mhlanga CHINHEYA ◽  
Murat YILMAZ ◽  
Aylin ÜSTÜNDAĞ ◽  
Seda İPEK ◽  
Yalçın DUYDU ◽  
...  
Keyword(s):  
Mouse Fibroblast ◽  
Fibroblast Cells ◽  
Genotoxic Effects ◽  
Pulp Capping ◽  
L929 Mouse Fibroblast ◽  
In Vitro Investigation ◽  
L929 Mouse

GLUT1 is associated with sphingolipid-organized, cholesterol-independent domains in L929 mouse fibroblast cells

Biochimie ◽  
2019 ◽  
Vol 162 ◽  
pp. 88-96
Author(s):  
Lauren E. Rylaarsdam ◽  
Grace N. Johnecheck ◽  
Brendan D. Looyenga ◽  
Larry L. Louters
Keyword(s):  
Mouse Fibroblast ◽  
Fibroblast Cells ◽  
L929 Mouse Fibroblast ◽  
L929 Mouse

scholarly journals Genotoxicity and cytotoxicity of mineral trioxide aggregate and calcium enriched mixture cements on L929 mouse fibroblast cells

2014 ◽  
Vol 33 (1) ◽  
pp. 64-69 ◽  
Author(s):  
Neda NAGHAVI ◽  
Jamileh GHODDUSI ◽  
Hamid R. SADEGHNIA ◽  
Elham ASADPOUR ◽  
Saeed ASGARY
Keyword(s):  
Mineral Trioxide Aggregate ◽  
Mouse Fibroblast ◽  
Fibroblast Cells ◽  
L929 Mouse Fibroblast ◽  
L929 Mouse

scholarly journals Cytotoxic Effects of Bulk-Fill Composites on L929 Fibroblast Cells

2021 ◽  
Vol 24 (1) ◽  
Author(s):  
Numan Aydın ◽  
Serpil Karaoğlanoğlu ◽  
Elif Aybala Oktay ◽  
Aysun Kılıç Süloğlu
Keyword(s):  
Cell Viability ◽  
Composite Resin ◽  
Mouse Fibroblast ◽  
Composite Resins ◽  
Control Group ◽  
Fibroblast Cells ◽  
Cytotoxic Effects ◽  
L929 Mouse Fibroblast ◽  
L929 Mouse ◽  
Composite Samples

Objective: Unlike traditional composite resins, bulk-fill composite resins could be polymerized as thicker layers. This study aims to contribute to the field by investigating the cytotoxic effects of various bulk-fill composite resins on L929 mouse fibroblast cells in vitro. Material and Methods: In our study, six bulk fill and one conventional composite resin were used. Composite resin samples (8×4 mm) were prepared in a sterile cabinet by using a glass mod and polymerizing with a led light device (DTE LUX E, Germany). Composite samples (n:3) of which surface area was calculated according to ISO 10993-12: 2012 standards (3 cm2/ml), were kept in media for 24 h and 72 h in 37 oC incubator, their extracts were filtered in 1:1 and 1:2 proportion and were added on L929 mouse fibroblast cells. Cell viability was examined by the MTT assay and cell death by the LDH test. Cell viability results were evaluated using one-way analysis of variance (ANOVA) test (p<0.05). Results: When the 1:1 extracts from 4 mm thick bulk-fill composite samples were applied on L929 mouse fibroblast cells, cell viability rates showed significant differences compared to the control group at the end of 24 h and 72 h (except for Estelite Bulk Fill Flow). Although the extracts of the tested composite samples at 1:1 and 1:2 ratio at the end of 72 hours caused a decrease in L929 mouse fibroblast cell viability, the cell viability rate of only PRG-containing bulk fill composite and conventional composite remained below the cell viability ratio (70%) specified in ISO standards. Bulk fill composites did not produce toxic effects (except Beautifil Bulk Restorative) according to the LDH test. Conclusions: Despite decreasing in general the cell viability, bulk-fill composite resins used in 4 mm thick layers provided cell viability rates over the acceptability level, except PRG-containing bulk fill composite (Beautifil Bulk Restorative), which was cytotoxic to L929 mouse fibroblasts. Keywords Bulk fill composite; Cytotoxicity; L929 cells; LDH assay.

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