scholarly journals Synthesis of the Macrolactone Cores of Maltepolides via a Diene–Ene Ring-Closing Metathesis Strategy

2021 ◽  
Author(s):  
Man Ki Sit ◽  
Hui Hui Cao ◽  
Yan-Dong Wu ◽  
Tsz Chun Yip ◽  
Lars Eric Bendel ◽  
...  
Keyword(s):  
Ring Opening ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Epoxide Ring ◽  
Fibroblast Cell Line ◽  
Ring Closing Metathesis ◽  
Double Bond Migration ◽  
L929 Mouse Fibroblast ◽  
Mouse Fibroblast Cell Line ◽  
L929 Mouse

Synthesis of the C19-truncated maltepolide E has been accomplished via a diene–ene RCM strategy without damage to the C11–C14 alkenyl epoxy unit. Upon release of the C17-OH group, it attacked at the C14 position with double bond migration and epoxide ring-opening to furnish the C19-truncated maltepolide A and B as proposed for the biosynthesis of maltepolides. Preliminary cytotoxicity data of the synthesized C19-truncated maltepolides against L929 mouse fibroblast cell line suggest irrelevance of the vinyl epoxide and importance of the conjugated dienyl keto unit for the observed anticancer activity.

scholarly journals Cytotoxicity of latex and non-latex orthodontic elastomeric ligatures on L929 mouse fibroblasts

2010 ◽  
Vol 21 (3) ◽  
pp. 205-210 ◽  
Author(s):  
Rogério Lacerda dos Santos ◽  
Matheus Melo Pithon ◽  
Fernanda Otaviano Martins ◽  
Maria Teresa Villela Romanos ◽  
Antônio Carlos de Oliveira Ruellas
Keyword(s):  
Cell Viability ◽  
Fibroblast Cell ◽  
Neutral Red ◽  
Mouse Fibroblast ◽  
Fibroblast Cell Line ◽  
Dye Uptake ◽  
L929 Mouse Fibroblast ◽  
Mouse Fibroblast Cell Line ◽  
Red Dye ◽  
L929 Mouse

This study investigated the cytotoxicity exists between latex and non-latex Orthodontic elastomeric ligatures. Six elastomeric ligatures (1 latex, 2 latex-free and 3 polyurethane) from different manufacturers were divided into 6 groups of 15 elastics each: A (Latex-free, American Orthodontics), M (Polyurethane, Morelli), G (Polyurethane,GAC International), Te (Polyurethane, Tecnident), TP (Natural latex,TP Orthodontics) and U (Latex-free,3M Unitek). The cytotoxicity assay was performed using cell cultures (L929 mouse fibroblast cell line), which were subjected to the cell viability test with neutral red ("dye-uptake") at 1, 2, 3, 7 and 28 days. Data were analyzed statistically by ANOVA and Tukey's test (α=0.05). No statistically significant differences (p>0.05) were observed between Groups M and Te in all experimental periods, except at 2 days. No significant differences (p>0.05) in cell viability were found either among Groups A, G, TP and U or between Groups M and Te at 24 h or among Groups CC, A, G, TP and U at 2 and 28 days. It may be concluded that latex-free elastomeric ligatures from American Orthodontics and Unitek trademarks induced less cell lysis compared to latex and polyurethane ligatures.

Rosette formation by a mouse fibroblast cell line

Nature ◽  
1974 ◽  
Vol 248 (5448) ◽  
pp. 514-515 ◽  
Author(s):  
E. V. ELLIOTT ◽  
R. S. KERBEL ◽  
B. J. PHILLIPS
Keyword(s):  
Cell Line ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Rosette Formation ◽  
Fibroblast Cell Line ◽  
Mouse Fibroblast Cell ◽  
Mouse Fibroblast Cell Line

scholarly journals Evaluation of cytotoxicity of 5-n-alkylresorcinol homologs and fraction on mouse fibroblast cell line L929

2016 ◽  
Vol 243 (7) ◽  
pp. 1137-1148 ◽  
Author(s):  
Izabela Biskup ◽  
Ewa Zaczynska ◽  
Miroslawa Krauze-Baranowska ◽  
Izabela Fecka
Keyword(s):  
Cell Line ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Fibroblast Cell Line ◽  
Mouse Fibroblast Cell ◽  
Mouse Fibroblast Cell Line

scholarly journals Cytotoxicity and Morphological Effects of Aqueous Areca Nut Crude Extract on L929 Fibroblast Cell Line

2020 ◽  
Vol 4 (1) ◽  
pp. 34-41
Author(s):  
Noor Fadilatul Akmal Mat Salleh ◽  
Ahmad Azlina ◽  
Masitah Hayati Harun ◽  
Badr Abdullah Al-Tayar ◽  
Siti Nurnasihah Md Hashim ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cell Line ◽  
Aqueous Extract ◽  
Crude Extract ◽  
L929 Cell ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Areca Nut ◽  
Fibroblast Cell Line ◽  
Mouse Fibroblast Cell Line

Betel quid chewing is a detrimental recreational habit amongst Asians and a risk factor for oral cancer. Arecoline, a component of areca nut (a major constituent of betel quid) is a known carcinogen. However, the effect of areca nut crude extract is not much studied. To evaluate the cytotoxicity and morphologic effects of areca nut aqueous extract on mouse fibroblast cell line (L929). Dried raw areca nut obtained from a local market in Kota Bharu, Kelantan was prepared and suspended in DMEM (Dulbecco’s Modified Eagle’s medium), prior to serial dilution of 1.56, 0.781, 0.39, 0.195, 0.0976, 0.0488, and 0.0244 mg/ml. The L929 was then exposed to each of the aqueous areca nut extract dilutions and incubated at 37 °C for 24, 48 and 72 hours. Following incubation, the cytotoxicity level of treated L929 was measured using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium] assay. Five highest concentrations of areca nut extract showed significantly decreased L929 cell viability (1.56, 0.781, 0.39, 0.195, 0.0976 mg/ml) for all incubation periods compared to untreated cells (p<0.05). The IC50 values of aqueous areca nut extract on L929 were 0.1516, 0.1040, and 0.09136 mg/ml at 24, 48 and 72 hours, respectively. The L929 cell showed altered morphology when cultured in the extract for 24 hours. Higher concentrations of the areca nut aqueous extract is cytotoxic to L929. Prolonged exposure to the extract reduced the IC50 value. Investigation on the role of areca nut in cell proliferation could be further undertaken to assess its association with oral cancer.       Keywords: areca nut, L929, mouse fibroblast cell line, cytotoxicity, MTT assay

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