scholarly journals Anti-cooperative Biphasic Equilibrium Binding of Transcription Factor Upstream Stimulatory Factor to Its Cognate DNA Monitored by Protein Fluorescence Changes

1995 ◽  
Vol 270 (33) ◽  
pp. 19325-19329 ◽  
Author(s):  
Ma Sha ◽  
Adrian R. Ferré-D'Amaré ◽  
Stephen K. Burley ◽  
Dixie J. Goss
Keyword(s):  
Transcription Factor ◽  
Upstream Stimulatory Factor ◽  
Protein Fluorescence ◽  
Equilibrium Binding ◽  
Stimulatory Factor

scholarly journals A yeast protein possesses the DNA-binding properties of the adenovirus major late transcription factor.

1989 ◽  
Vol 9 (2) ◽  
pp. 820-822 ◽  
Author(s):  
L A Chodosh ◽  
S Buratowski ◽  
P A Sharp
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Binding Site ◽  
Upstream Stimulatory Factor ◽  
Binding Properties ◽  
Yeast Saccharomyces Cerevisiae ◽  
Human Promoter ◽  
Late Transcription ◽  
Stimulatory Factor ◽  
Dna Binding Properties

The adenovirus major late transcription factor (MLTF), or upstream stimulatory factor, is a human promoter-specific transcription factor which recognizes the near-palindromic sequence GGCCACGTGACC (R. W. Carthew, L. A. Chodosh, and P. A. Sharp, Cell 43:439-448, 1985; L. A. Chodosh, R. W. Carthew, and P. A. Sharp, Mol. Cell. Biol. 6:4723-4733, 1986; M. Sawadogo and R. G. Roeder, Cell 43:165-175, 1985). We describe here a protein found in the yeast Saccharomyces cerevisiae which possesses DNA-binding properties that are virtually identical to those of human MLTF. These two proteins recognize the same DNA-binding site, make the same purine nucleotide contacts, and are affected in the same manner by mutations in the MLTF-binding site.

An auxiliary peptide required for the function of two activation domains in upstream stimulatory factor 2 (USF2) transcription factor

1997 ◽  
Vol 1 (2) ◽  
pp. 87-97 ◽  
Author(s):  
Laurence Gourdon ◽  
Anne-Marie Lefrançois-Martinez ◽  
Benoît Viollet ◽  
Antoine Martinez ◽  
Axel Kahn ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Upstream Stimulatory Factor ◽  
Activation Domains ◽  
Stimulatory Factor

scholarly journals Cyclin-Dependent Kinase 5 (CDK5)-Mediated Phosphorylation of Upstream Stimulatory Factor 2 (USF2) Contributes to Carcinogenesis

Cancers ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 523 ◽  
Author(s):  
Tabughang Chi ◽  
Tina Horbach ◽  
Claudia Götz ◽  
Thomas Kietzmann ◽  
Elitsa Dimova
Keyword(s):  
Transcription Factor ◽  
Cancer Cells ◽  
Tumor Development ◽  
Cellular Growth ◽  
Regulatory Mechanisms ◽  
Cyclin Dependent Kinase ◽  
Upstream Stimulatory Factor ◽  
And Migration ◽  
Stimulatory Factor ◽  
Cyclin Dependent Kinase 5

The transcription factor USF2 is supposed to have an important role in tumor development. However, the regulatory mechanisms contributing to the function of USF2 are largely unknown. Cyclin-dependent kinase 5 (CDK5) seems to be of importance since high levels of CDK5 were found in different cancers associated with high USF2 expression. Here, we identified USF2 as a phosphorylation target of CDK5. USF2 is phosphorylated by CDK5 at two serine residues, serine 155 and serine 222. Further, phosphorylation of USF2 at these residues was shown to stabilize the protein and to regulate cellular growth and migration. Altogether, these results delineate the importance of the CDK5-USF2 interplay in cancer cells.

A yeast protein possesses the DNA-binding properties of the adenovirus major late transcription factor

1989 ◽  
Vol 9 (2) ◽  
pp. 820-822
Author(s):  
L A Chodosh ◽  
S Buratowski ◽  
P A Sharp
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Binding Site ◽  
Upstream Stimulatory Factor ◽  
Binding Properties ◽  
Yeast Saccharomyces Cerevisiae ◽  
Human Promoter ◽  
Late Transcription ◽  
Stimulatory Factor ◽  
Dna Binding Properties

The adenovirus major late transcription factor (MLTF), or upstream stimulatory factor, is a human promoter-specific transcription factor which recognizes the near-palindromic sequence GGCCACGTGACC (R. W. Carthew, L. A. Chodosh, and P. A. Sharp, Cell 43:439-448, 1985; L. A. Chodosh, R. W. Carthew, and P. A. Sharp, Mol. Cell. Biol. 6:4723-4733, 1986; M. Sawadogo and R. G. Roeder, Cell 43:165-175, 1985). We describe here a protein found in the yeast Saccharomyces cerevisiae which possesses DNA-binding properties that are virtually identical to those of human MLTF. These two proteins recognize the same DNA-binding site, make the same purine nucleotide contacts, and are affected in the same manner by mutations in the MLTF-binding site.

Sign in / Sign up

Export Citation Format

Share Document