scholarly journals Transcriptional Analysis of the Glutamate Dehydrogenase Gene in the Primitive Eukaryote,Giardia lamblia

2000 ◽  
Vol 275 (15) ◽  
pp. 11432-11439 ◽  
Author(s):  
Janet Yee ◽  
Michael R. Mowatt ◽  
Patrick P. Dennis ◽  
Theodore E. Nash
Keyword(s):  
Glutamate Dehydrogenase ◽  
Giardia Lamblia ◽  
Transcriptional Analysis ◽  
Dehydrogenase Gene

scholarly journals Molecular detection of glutamate dehydrogenase gene of Giardia lamblia isolated from food handlers in Erbil city

2021 ◽  
Vol 25 (1) ◽  
pp. 431-437
Author(s):  
Hataw Saber ◽  
Hawri Bakr
Keyword(s):  
Glutamate Dehydrogenase ◽  
Nested Pcr ◽  
Giardia Lamblia ◽  
Diarrheal Disease ◽  
Asymptomatic Carrier ◽  
Positive Sample ◽  
Pcr Analysis ◽  
Food Handler ◽  
Food Handlers ◽  
Dehydrogenase Gene

Background and objective: Giardia lamblia is the intestinal flagellated protozoan parasite that infects vertebrates, including humans. Giardiasis is the major diarrheal disease found worldwide. It can be symptomatic or may be an asymptomatic carrier that led to chronic disease. This study aimed to determine the proportion of giardiasis among food handlers and evaluate the correlation between two laboratory methods for identifying the Giardia lamblia. Methods: A total of 308 stool samples were collected from food handlers that annually attend the central laboratory in Erbil City. Wetmount microscopic examination was performed for the diagnosis of cysts and trophozoites of the Giardia parasite. Molecular analysis done for positive samples, DNA extraction performed using the QIAamp Fast DNA Stool Mini Kit (Qiagen Company, Germany). Nested PCR analysis was done targeting the Glutamate dehydrogenase gene using two sets of primers for amplification of 734bp fragment. Gel electrophoresis was performed for visualizing the amplified DNA by Ultraviolet light. Results: The mean age of food handler participants was 29 years. Most (93.8%) of the food handlers were males, and the majority (98.6%) of the participants did not have any signs and symptoms. Four (7.4%) microscopy positive sample participants were highly educated. There was no association between educational level and positive rate by microscopy (P = 0.066). The majority of participants did not receive treatments, particularly most of the microscopic positive samples. The food handlers did not take any antiparasitic treatments 9 (3.4%) (P = 0.676). From 11 (3.6%) microscopically positive samples, 10 (90.9%) Giardia lamblia gdh gene 734 bp fragments were amplified by nested PCR. Conclusion: Amplification of 734bp of gdh gene by the nested PCR is the most specific and sensitive method for identifying Giardia lamblia. Food handlers were important people to care about sanitation and preparing food, particularly for avoiding diseases transmitted by food. Keywords: Giardiasis; Genetic characterization; gdh gene; Nested PCR.

scholarly journals A glutamate dehydrogenase gene sequence

1989 ◽  
Vol 17 (24) ◽  
pp. 10500-10500 ◽  
Author(s):  
J. Mark Cock ◽  
Robert R. Schmidt
Keyword(s):  
Glutamate Dehydrogenase ◽  
Gene Sequence ◽  
Dehydrogenase Gene

Glucocorticoid upregulation of glutamate dehydrogenase gene expression in vitro in astrocytes

1996 ◽  
Vol 37 (1-2) ◽  
pp. 324-328 ◽  
Author(s):  
H. Hardin-Pouzet ◽  
P. Giraudon ◽  
M.F. Belin ◽  
M. Didier-Bazes
Keyword(s):  
Gene Expression ◽  
Glutamate Dehydrogenase ◽  
Dehydrogenase Gene

scholarly journals Using RFLP-PCR Technique in Determining Genotypes of Giardia lamblia from Diarrhea Cases in Children in AL-Diwaniyah City, Iraq

2020 ◽  
Vol 1 (2) ◽  
pp. 29-34
Author(s):  
Hadi M. Hamza AL-Mayali ◽  
Lubna Abdul-Kadir AL-Ibrahim
Keyword(s):  
Rural Areas ◽  
Giardia Lamblia ◽  
Urban Areas ◽  
Age Groups ◽  
Intestinal Protozoa ◽  
Gender And Age ◽  
Dehydrogenase Gene ◽  
Urban And Rural Areas ◽  
Child Hospital ◽  
Genotype A

Introduction: Giardia lamblia is one of the most prevalent intestinal protozoa in the world, which affect children in both undeveloped and developing countries. This study aimed to determine genotypes of the Giardia lamblia using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP)-PCR techniques. Additionally, the relationship between genotype patterns and their geographical distribution, gender, and age was investigated. Methods: The current study included 926 samples of faeces of children suffering from diarrhoea, who visits the internal clinics at Teaching Hospital, and Child Hospital in AL- Diwaniyah City from November 2012 - Jun 2013. For age groups of 1-12 years exclusively. The samples were examined using a direct mount wet smear, The positive samples were preserved without adding preservatives at a temperature of -20°C until the DNA extraction for G. lamblia genotyping by using PCR and RFLP-PCR technique. Results: Giardia lamblia was detected in 2.15% (20/926) of samples from diarrhea cases in children by amplification of glutamate dehydrogenase gene (gdh) using two specific primers GDHiR and GDHiF. It was revealed that 7 samples belonged to genotype A (35%) and 13 samples belonged to genotype B (65%). All genotype A samples belonged to subgenotype AII (100%), while genotype B samples belonged to subgenotypes BIII (53.61 %) and BIV (47.38 %). Genotype A was detected in children of 1-6 years of age while B genotype was detected in all age groups. Both of the genotypes have been detected in both genders (male and female) and genotype B was found in both urban and rural areas; however, its prevalence was higher in rural areas than in urban areas (100% and 30%, respectively). Conclusion: There are two genotypes of Giardia lamblia, genotype A and genotype B, each of which has secondary genetic patterns which include AII, BIII, and BIV.

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