scholarly journals Confocal Laser Raman Microspectroscopy of Biomineralization Foci in UMR 106 Osteoblastic Cultures Reveals Temporally Synchronized Protein Changes Preceding and Accompanying Mineral Crystal Deposition

2008 ◽  
Vol 284 (11) ◽  
pp. 7100-7113 ◽  
Author(s):  
Chuanyi Wang ◽  
Yong Wang ◽  
Nichole T. Huffman ◽  
Chaoying Cui ◽  
Xiaomei Yao ◽  
...  
Keyword(s):  
Raman Microspectroscopy ◽  
Confocal Laser ◽  
Crystal Deposition ◽  
Laser Raman ◽  
Mineral Crystal ◽  
Umr 106

scholarly journals Multimolecular Salivary Mucin Complex Is Altered in Saliva of Cigarette Smokers: Detection of Disulfide Bridges by Raman Spectroscopy

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Motoe Taniguchi ◽  
Junko Iizuka ◽  
Yukari Murata ◽  
Yumi Ito ◽  
Mariko Iwamiya ◽  
...  
Keyword(s):  
Disulfide Bonds ◽  
Raman Microspectroscopy ◽  
Confocal Laser ◽  
Oral Epithelium ◽  
Amino Acid Residues ◽  
Disulfide Bridges ◽  
Cigarette Smokers ◽  
Laser Raman ◽  
Salivary Mucin ◽  
Premenopausal Female

Saliva contains mucins, which protect epithelial cells. We showed a smaller amount of salivary mucin, both MG1 and MG2, in the premenopausal female smokers than in their nonsmoking counterparts. Smokers' MG1, which contains almost 2% cysteine/half cystine in its amino acid residues, turned out to be chemically altered in the nonsmoker’s saliva. The smaller acidic glycoprotein bands were detectable only in smoker’s saliva in the range of 20–25 kDa and at 45 kDa, suggesting that degradation, at least in part, caused the reduction of MG1 mucin. This is in agreement with the previous finding that free radicals in cigarette smoke modify mucins in both sugar and protein moieties. Moreover, proteins such as amylase and albumin are bound to other proteins through disulfide bonds and are identifiable only after reduction with DTT. Confocal laser Raman microspectroscopy identified a disulfide stretch band of significantly stronger intensity per protein in the stimulated saliva of smokers alone. We conclude that the saliva of smokers, especially stimulated saliva, contains significantly more oxidized form of proteins with increased disulfide bridges, that reduces protection for oral epithelium. Raman microspectroscopy can be used for an easy detection of the damaged salivary proteins.

Application of confocal laser Raman spectroscopy on marine sediment microplastics

2020 ◽  
Vol 38 (5) ◽  
pp. 1502-1516
Author(s):  
Jing Liu ◽  
Xin Zhang ◽  
Zengfeng Du ◽  
Zhendong Luan ◽  
Lianfu Li ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Marine Sediment ◽  
Laser Raman Spectroscopy ◽  
Confocal Laser ◽  
Laser Raman

Confocal Laser Raman Microprobe Studies of Keratin Fibers

1995 ◽  
Vol 65 (10) ◽  
pp. 593-600 ◽  
Author(s):  
Lucia E. Jurdana ◽  
Kenneth P. Ghiggino ◽  
Kerry W. Nugent ◽  
Ian H. Leaver
Keyword(s):  
Confocal Laser ◽  
Laser Raman ◽  
Raman Microprobe ◽  
Keratin Fibers

scholarly journals Guangzhou Tongcao painting in late China Qing Dynasty（1840—1912 AD）: technology revealed by Analytical Approaches

2020 ◽  
Author(s):  
Chan Zhang ◽  
Jianbin Huang ◽  
Tiequan Zhu ◽  
Ronglin Zhang
Keyword(s):  
Prussian Blue ◽  
Qing Dynasty ◽  
Polarized Light ◽  
Confocal Laser ◽  
Western World ◽  
Late Qing Dynasty ◽  
Laser Raman ◽  
Stereo Microscope ◽  
Synthetic Pigments ◽  
Analytical Approaches

Abstract Guangzhou Tongcao painting in Qing Dynasty of China witnessed the exchange of economy, culture, art and technology between China and foreign countries, which was an important exported product in historical China. In this paper, by using Optical Stereo Microscope, Polarized Light Microscope, Scanning Electron Microscope attached Energy Dispersive Spectroscopy and micro confocal laser Raman Spectroscope, a Qing Dynasty Tongcao painting with the subject of family life of an official's female relative was analyzed. Our study revealed that: 1) the hexagonal cell morphology which was easily observed in the Tongcao pith could contain more pigments and increase the stereo sense of painting, and the cell of historical Tongcao pith had started to degrade, which indicated an urgent requirement on conservation of Tongcao painting; 2) alum was needed as the fixing agent in the preparation of pigments and the painting process; 3) both the China domestic pigments including red lead, calcite, lead white, carbon black and gamboge, and the synthetic pigments imported from Europe (synthetic ultramarine, prussian blue and emerald green) were applied in this painting, which reflected the bi-directional communication of culture and technology between China and western world. Meanwhile, the existence of synthetic ultramarine, prussian blue and emerald green was helpful to further identify the production date of this painting to late Qing Dynasty, mainly the mid-late 19th century.

scholarly journals Multi-Analytical Research on the Caisson Painting of Dayu Temple in Hancheng, Shaanxi, China

Coatings ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1372
Author(s):  
Jiaxin Li ◽  
Bingjie Mai ◽  
Peng Fu ◽  
Gele Teri ◽  
Yanli Li ◽  
...  
Keyword(s):  
Energy Dispersive Spectrometer ◽  
Optical Microscope ◽  
Scientific Data ◽  
Depth Of Field ◽  
Confocal Laser ◽  
Animal Glue ◽  
Analytical Methodology ◽  
X Ray ◽  
Laser Raman ◽  
Corroborative Evidence

In this research, the caisson painting of Dayu Temple in Hancheng, Shaanxi, China, was analyzed via a multi-analytical methodology, using a pH meter, an ultra-depth-of-field optical microscope, a scanning electron microscope–energy dispersive spectrometer (SEM-EDS), a high-resolution X-ray diffractometer (XRD), a micro-confocal laser Raman spectrometer, a gas chromatography mass spectrometer (GC-MS), and X-ray fluorescence spectroscopy (XRF). With the corroborative evidence derived from the above analyses, it could be determined that the caisson painting of Dayu Temple was painted on bamboo paper and attached to hemlock wood substrate of the Pinaceae Tsuga genus using starch paste, with common colorants such as carbon black, cinnabar mixed with a small amount of red lead, ultramarine, and ultramarine mixed with Paris green, with animal glue having been adopted as a sizing agent. These results provide important scientific data for the production craft of precious caisson paintings, contributing to the revelation of their historic, artistic, and scientific value, and should enable conservators to make informed decisions in restoration.

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