scholarly journals Targeted Quantitative Analysis ofStreptococcus pyogenesVirulence Factors by Multiple Reaction Monitoring

2008 ◽  
Vol 7 (8) ◽  
pp. 1489-1500 ◽  
Author(s):  
Vinzenz Lange ◽  
Johan A. Malmström ◽  
John Didion ◽  
Nichole L. King ◽  
Björn P. Johansson ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Multiple Reaction Monitoring ◽  
Reaction Monitoring

Determination and validation of aprepitant in rat plasma using LC−MS/MS

Bioanalysis ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 363-372
Author(s):  
Nazlı Erdoğar ◽  
Tuba Reçber ◽  
Alper B İskit ◽  
Erem Bilensoy ◽  
Sedef Kır ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Formic Acid ◽  
Multiple Reaction Monitoring ◽  
Rat Plasma ◽  
Pharmacokinetic Parameters ◽  
Reaction Monitoring ◽  
Bioanalytical Method Validation ◽  
Bioanalytical Method ◽  
Acceptable Range ◽  
Multiple Reaction Monitoring Mode

Aim: The assessment of efficacy should be paralleled with extensive pharmacokinetic parameters, and a valid bioanalytical method is a pre-condition for accurate plasma concentration. Materials & methods: A simple, specific, rapid and sensitive LC−MS/MS method has been developed for quantitative analysis of aprepitant in rat plasma. A C18 column was used as stationary phase and the mobile phase consisted of a mixture of formic acid in water and formic acid in acetonitrile. Quantification was performed using multiple reaction monitoring mode. Results: The selectivity, linearity, accuracy, precision, robustness and ruggedness of the method were evaluated in accordance with bioanalytical method validation guideline of ICH and all results were within the acceptable range. Conclusion: The validated LC−MS/MS method was found to be useful for the quantitative analysis of aprepitant in rat plasma samples.

scholarly journals EISA-MRM: Multiple Reaction Monitoring with Single Quadrupole Mass Spectrometry

2021 ◽  
Author(s):  
Jingchuan Xue ◽  
Rico J.E. Derks ◽  
William Wwebb ◽  
Aries Aisporna ◽  
Martin Giera ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Dynamic Range ◽  
Matrix Effects ◽  
Multiple Reaction Monitoring ◽  
Mass Spectrometry Analysis ◽  
Reaction Monitoring ◽  
Quadrupole Mass ◽  
Fragment Ions ◽  
Specificity And Sensitivity

<p>Enhanced in-source fragmentation annotation combined with single quadrupole multiple reaction monitoring (EISA-MRM) has been designed for quantitative mass spectrometry analysis. EISA contrasts to traditional electrospray as a soft ionization technology and is proving to be advantageous since the resulting fragment ions are identical as those generated in tandem mass spectrometry. Criteria established by the European Union Commission Directive 2002/657/EC for electron ionization single quadrupole quantitative analysis was used for the EISA-MRM quantitative analyses and experiments were performed on multiple types of complex samples that included a mixture of 50 standards, as well as cell and plasma extracts. The dynamic range for quantitative analysis was comparable to QqQ-MRM analyses at up to 5 orders of magnitude and the EISA-MRM and QqQ-MRM of the cell and plasma extracts showed similar matrix effects. Amino acid and fatty acid measurements performed from certified NIST 1950 plasma with isotopically labelled standards demonstrated EISA-MRM accuracy in the range of 91-110% for the amino acids, 76-129% for the fatty acids, and precision with a CV < 10%. In order to enhance specificity and sensitivity, a newly developed Correlated SIM Chromatogram (CSC) algorithm was designed to facilitate MRM quality analyses. The EISA-MRM quantitative analysis with CSC informatics enables both precursor and in-source fragment ions to be correlated within a single quadrupole mass spectrometer.</p>

scholarly journals Single Quadrupole Multiple Reaction Monitoring Q-MRM enables Quantitative Mass Spectrometry

2021 ◽  
Author(s):  
Jingchuan Xue ◽  
Rico J.E. Derks ◽  
William Wwebb ◽  
Aries Aisporna ◽  
Martin Giera ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Matrix Effects ◽  
Multiple Reaction Monitoring ◽  
Mass Spectrometry Analysis ◽  
Good Precision ◽  
Reaction Monitoring ◽  
Font Size ◽  
Quantitative Mass Spectrometry ◽  
Fragment Ions

<p> </p><p>A single quadrupole combined with enhanced in-source fragmentation/annotation (EISA) was used to perform multiple reaction monitoring (Q-MRM) for quantitative mass spectrometry analysis. EISA amplifies fragmentation of traditional soft electrospray ionization to produce fragment ions that have been found to be identical to those generated in tandem mass spectrometry. We have combined EISA fragmentation data with criteria established by the European Union Commission Directive 2002/657/EC for electron ionization single quadrupole quantitative analysis to perform quantitative Q-MRM experiments. These experiments were performed on multiple types of complex samples that included a mixture of 50 standards, as well as cell and plasma extracts. The dynamic range for Q-MRM quantitative analysis was comparable to triple quadrupole multiple reaction monitoring (QqQ-MRM) analyses at up to 5 orders of magnitude with the cell and plasma extracts showing similar matrix effects across both platforms. Amino acid and fatty acid measurements performed from certified NIST 1950 plasma with isotopically labelled standards demonstrated Q-MRM accuracy in the range of 91-110% for the amino acids, 76-129% for the fatty acids, and good precision (coefficient of variation < 10%). In order to enhance specificity, a newly developed Correlated Ion Monitoring (CIM) algorithm was designed to facilitate these analyses. CIM autonomously processes, aligns, filters, and compiles multiple ions within one chromatogram enabling both precursor and in-source fragment ions to be correlated within a single chromatogram, also enabling the detection of co-eluting species based on precursor and fragment ion ratios. Q-MRM and CIM with single quadrupole instrumentation has been designed as an alternative to QqQ-MRM technology by correlating precursor and fragment ions to facilitate high sensitivity Q-MRM quantitative analysis on existing instrumentation that are generally inexpensive, easy to operate, and technically less complex. </p> <p>@font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:3 0 0 0 1 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0in; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman";}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:11.0pt; mso-ansi-font-size:11.0pt; mso-bidi-font-size:11.0pt; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:DengXian; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-fareast-language:ZH-CN;}.MsoPapDefault {mso-style-type:export-only; margin-bottom:8.0pt; line-height:107%;}div.WordSection1 {page:WordSection1;}</p>

scholarly journals EISA-MRM: Multiple Reaction Monitoring with Single Quadrupole Mass Spectrometry

2021 ◽  
Author(s):  
Jingchuan Xue ◽  
Rico J.E. Derks ◽  
William Wwebb ◽  
Aries Aisporna ◽  
Martin Giera ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Dynamic Range ◽  
Matrix Effects ◽  
Multiple Reaction Monitoring ◽  
Mass Spectrometry Analysis ◽  
Reaction Monitoring ◽  
Quadrupole Mass ◽  
Fragment Ions ◽  
Specificity And Sensitivity

<p>Enhanced in-source fragmentation annotation combined with single quadrupole multiple reaction monitoring (EISA-MRM) has been designed for quantitative mass spectrometry analysis. EISA contrasts to traditional electrospray as a soft ionization technology and is proving to be advantageous since the resulting fragment ions are identical as those generated in tandem mass spectrometry. Criteria established by the European Union Commission Directive 2002/657/EC for electron ionization single quadrupole quantitative analysis was used for the EISA-MRM quantitative analyses and experiments were performed on multiple types of complex samples that included a mixture of 50 standards, as well as cell and plasma extracts. The dynamic range for quantitative analysis was comparable to QqQ-MRM analyses at up to 5 orders of magnitude and the EISA-MRM and QqQ-MRM of the cell and plasma extracts showed similar matrix effects. Amino acid and fatty acid measurements performed from certified NIST 1950 plasma with isotopically labelled standards demonstrated EISA-MRM accuracy in the range of 91-110% for the amino acids, 76-129% for the fatty acids, and precision with a CV < 10%. In order to enhance specificity and sensitivity, a newly developed Correlated SIM Chromatogram (CSC) algorithm was designed to facilitate MRM quality analyses. The EISA-MRM quantitative analysis with CSC informatics enables both precursor and in-source fragment ions to be correlated within a single quadrupole mass spectrometer.</p>

scholarly journals Polarity-extended quantitative analysis of bear bile and its analogues using serially coupled reversed phase-hydrophilic interaction liquid chromatography-tailored multiple reaction monitoring

RSC Advances ◽  
2017 ◽  
Vol 7 (83) ◽  
pp. 52822-52831 ◽  
Author(s):  
Qingqing Song ◽  
Wenjing Liu ◽  
Yu Yan ◽  
Peng Li ◽  
Jun Li ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Multiple Reaction Monitoring ◽  
Reversed Phase ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Reaction Monitoring ◽  
Hydrophilic Interaction ◽  
Bear Bile

Polarity-extended quantitative analysis of bear bile and its analogues was achieved using serially coupled reversed phase-hydrophilic interaction liquid chromatography-tailored multiple reaction monitoring.

Potential of hyphenated ultra-high performance liquid chromatography-scheduled multiple reaction monitoring algorithm for large-scale quantitative analysis of traditional Chinese medicines

RSC Advances ◽  
2015 ◽  
Vol 5 (71) ◽  
pp. 57372-57382 ◽  
Author(s):  
Qingqing Song ◽  
Yuelin Song ◽  
Na Zhang ◽  
Jun Li ◽  
Yong Jiang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
High Performance ◽  
Large Scale ◽  
Multiple Reaction Monitoring ◽  
Core Shell ◽  
Traditional Chinese Medicines ◽  
Reaction Monitoring ◽  
High Quality ◽  
Chinese Medicines ◽  
Monitoring Algorithm

The combination of a core–shell ADME column with a sMRM algorithm offers the potential for large-scale quantitative analysis of the components in TCMs by providing not only high quality quantitative signals but also reliable MS2 spectra.

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