Isoelectric focusing of proteins.

Root Knot Nematodes ◽

Abstract This chapter focuses on the efficacy of isoelectric focusing, which is a technique used for separating charged molecules by differences in their isoelectric point, in the examination of general proteins and specific enzymatic differences among plant parasitic nematodes, particularly the potato cyst and root knot nematodes.

Isoelectric focusing of proteins.

Techniques for work with plant and soil nematodes ◽

10.1079/9781786391759.0206 ◽

2021 ◽

pp. 206-217

Author(s):

Janete A. Brito

Keyword(s):

Isoelectric Focusing ◽

Isoelectric Point ◽

Parasitic Nematodes ◽

Root Knot Nematodes ◽

Marigolds (Tagetes spp.) for Nematode Management

EDIS ◽

10.32473/edis-ng045-2007 ◽

2007 ◽

Vol 2007 (19) ◽

Author(s):

R. Krueger ◽

K. E. Dover ◽

Robert McSorley ◽

K. H. Wang

Keyword(s):

Mode Of Action ◽

Cover Crop ◽

Parasitic Nematodes ◽

Root Knot Nematodes ◽

Fact Sheet ◽

Nematode Management ◽

ENY-056, an 8-page fact sheet by R. Krueger, K. E. Dover, R. McSorley, and K. -H. Wang, introduces homeowners to the problem of root-knot nematodes, the use of marigolds as an allelopathic cover crop for nematode suppression. It describes the mode of action, planting tips, considerations, and frequently asked questions. Includes references and tables showing susceptibility of marigold varieties to root-knot and plant-parasitic nematodes in Florida. Published by the UF Department of Entomology and Nematology, August 2007. ENY-056/NG045: Marigolds (Tagetes spp.) for Nematode Management (ufl.edu)

Occurrence of plant parasitic nematodes and non‐pathogenic species ofFusariumin tomato plants in Kenya and their role as mutualistic synergists for biological control of root‐knot nematodes

International Journal of Pest Management ◽

10.1080/09670879409371907 ◽

1994 ◽

Vol 40 (4) ◽

pp. 321-325 ◽

Cited By ~ 40

Author(s):

J. Hallmann ◽

R. A. Sikora

Keyword(s):

Biological Control ◽

Parasitic Nematodes ◽

Pathogenic Species ◽

Root Knot Nematodes ◽

Tomato Plants ◽

Community Analysis of Nematodes Associated with Banana, Identification of root knot nematode and Evaluation the Susceptibility of Some Cultivars to Infection

10.21203/rs.3.rs-740928/v1 ◽

2021 ◽

Author(s):

Radwa G. Mostafa ◽

Aida M. El-Zawahry ◽

Ashraf E. M. Khalil ◽

Ameer E. Elfarash ◽

Ali D. A. Allam

Keyword(s):

Meloidogyne Javanica ◽

Nematode Species ◽

Common Species ◽

Molecular Techniques ◽

Control Measures ◽

Parasitic Nematodes ◽

Root Knot Nematode ◽

Root Knot Nematodes ◽

Abstract Background Plant-parasitic nematodes are extremely dangerous pests in a variety of economically important crops. The purpose of this study was a survey of all nematode species existing in banana from three sites in Assiut Governorate, Egypt and to characterize the most common species by morphological, morphometric and molecular techniques (PCR with species-specific primers). Then, study of resistance or sensitivity of some banana cultivars to root-knot nematodes.Methods and Results Four nematodes, Meloidogyne, Rotylenchulus reniformis, Helicotylenchus and Pratylenchus were isolated and identified from soil and root samples collected from banana plants. Most frequently occurring of plant parasitic nematode species in banana was Meloidogyne. Former research found differences in species and in resistance to root-knot nematodes among the examined plant cultivars. Identification of Root-knot nematodes by Characterize of morphometric, molecularly, morphological isolate of Meloidogyne related to banana plants. The results revealed that the identified nematode species, Meloidogyne javanica, is the most common plant-parasitic nematodes in all locations. Data on the susceptibility of the tested banana cultivars to M. javanica revealed that Grand Naine was highly susceptible (HS) however, Magraby was susceptible (S) but Williams and Hindi cultivars were moderately resistant (MR).Conclusions we concluded that a survey revealed the significant prevalence of Meloidogyne javanica, the most important nematodes on banana in Assiut. The morphometric, morphological, and molecular identification were harmonic with one another. In addition to the host response of certain banana cultivars, to M. javanica that resistance is of significance and can be helpful to incorporate through planning control measures for root- knot nematodes.

Attachment tests of Pasteuria penetrans to the cuticle of plant and animal parasitic nematodes, free living nematodes and srf mutants of Caenorhabditis elegans

Journal of Helminthology ◽

10.1017/s0022149x99000098 ◽

1999 ◽

Vol 73 (1) ◽

pp. 67-71 ◽

Cited By ~ 15

Author(s):

P. Mendoza de Gives ◽

K.G. Davies ◽

M. Morgan ◽

J.M. Behnke

Keyword(s):

Caenorhabditis Elegans ◽

Parasitic Nematodes ◽

Pasteuria Penetrans ◽

Cyst Nematodes ◽

Free Living ◽

Root Knot Nematodes ◽

C Elegans ◽

Spore Attachment ◽

Populations of Pasteuria penetrans isolated from root-knot nematodes (Meloidogyne spp.) and cyst nematodes (Heterodera spp.) were tested for their ability to adhere to a limited selection of sheathed and exsheathed animal parasitic nematodes, free living nematodes, including Caenorhabditis elegans wild type and several srf mutants, and plant parasitic nematodes. The attachment of spores of Pasteuria was restricted and no spores were observed adhering to any of the animal parasitic nematodes either with or without their sheath or to any of the free living nematodes including C. elegans and the srf mutants. All spore attachment was restricted to plant parasitic nematodes; however, spores isolated from cyst nematodes showed the ability to adhere to other genera of plant parasitic nematodes which was not the case with spores isolated from root-knot nematodes. The results are discussed in relationship to cuticular heterogeneity.

Plant-Parasitic Nematodes Infecting Grapevine in Southern Spain and Susceptible Reaction to Root-Knot Nematodes of Rootstocks Reported as Moderately Resistant

Plant Disease ◽

10.1094/pdis-91-9-1147 ◽

2007 ◽

Vol 91 (9) ◽

pp. 1147-1154 ◽

Cited By ~ 18

Author(s):

Daniel Téliz ◽

Blanca B. Landa ◽

Hava F. Rapoport ◽

Fernando Pérez Camacho ◽

Rafael M. Jiménez-Díaz ◽

...

Keyword(s):

Vascular System ◽

Southern Spain ◽

Parasitic Nematodes ◽

Histopathological Study ◽

Population Densities ◽

Root Knot Nematodes ◽

Meloidogyne Spp ◽

Plant Parasitic ◽

Susceptible Response

Incidence and nematode population densities of plant-parasitic nematodes were determined in 64 samples of soil and grapevine roots collected from commercial vineyards in southern Spain between October 2003 and May 2005. In addition, a histopathological study was done of root-stock roots naturally infected by root-knot nematodes (Meloidogyne spp.). Nematodes infecting the rootstocks were identified according to conventional procedures, and the Meloidogyne spp. were furthermore identified by sequence characterized amplified region-polymerase chain reaction (SCAR-PCR) and isozyme esterase analyses. The most important plant-parasitic nematodes detected, in order of decreasing frequency of total soil infestation and root infection (percentage of samples), were Mesocriconema xenoplax (34.4%), Meloidogyne incognita (26.6%), Meloidogyne javanica (14.1%), Xiphinema index (12.5%), Xiphinema italiae (10.9%), Pratylenchus vulnus (6.3%), and Meloidogyne arenaria (1.6%). No disease symptoms were observed on aboveground plant parts of the infected grapevines, except for plants in some fields where soil was infested with the virus-vector nematodes X. index and X. italiae. Those grapevines showed a yellow mosaic pattern in leaves early in the growing season and the internode shortening characteristic of infections by Grapevine fanleaf virus. Rootstocks infected by root-knot nematodes (Meloidogyne spp.) showed distorted feeder roots and large- to moderate-sized root galls, present either singly or in clusters. Histopathology of galled roots showed a typical susceptible response to infection by root-knot nematodes: cellular alterations were induced in the cortex, endodermis, pericycle, and vascular system, including giant-cell formation and severe distortion of vascular tissues. Most Meloidogyne egg masses ocurred on the surface of the galled root tissues, a position that could facilitate dispersion of the nematode eggs and juveniles and the occurrence of secondary infections. Some of the grapevine rootstocks surveyed in this study (Paulsen 1103, Richter 110, Rupestris du Lot, and SO4) had previously been reported to be resistant to Meloidogyne spp.; however, the population densities of these nematodes found in soil and roots sampled in the present study, as well as the compatible host-parasite relationship revealed by histopathology, indicate a susceptible response to Meloidogyne spp. from southern Spain.

Sedentary endoparasitic nematodes as a model for other plant parasitic nematodes

Nematology ◽

10.1163/156854100508827 ◽

2000 ◽

Vol 2 (1) ◽

pp. 113-121 ◽

Cited By ~ 11

Author(s):

Godelieve Gheysen ◽

Jan De Meutter ◽

Tom Tytgat ◽

August Coomans

Keyword(s):

Plant Root ◽

Complex Interaction ◽

The Other ◽

Parasitic Nematodes ◽

Root Knot Nematodes ◽

Plant Parasitism ◽

Severe Reduction ◽

Different Types ◽

AbstractPlant parasitic nematodes are known to cause a severe reduction in crop yield. Recently much effort is being put to engineering new nematode-resistant crop cultivars. Plant parasitic nematodes occur in three widely separated orders: Triplonchida, Dorylaimida and Tylenchida. All triplonchid and dorylaimid plant parasitic nematodes are migratory ectoparasites of roots. Within the Tylenchida, several different types of plant parasitism can be recognised. The sedentary endoparasites have the most complex interaction with their host, and are responsible for the vast majority of the agricultural damage. This causes most research to be concentrated on two groups of the sedentary endoparasitic nematodes: cyst- and root-knot nematodes. Both induce specialised feeding structures in the vascular cylinder of the plant root. The mechanism of phytoparasitism of the cyst- and root-knot nematodes is reviewed, of which some aspects will be applicable to the study of the other plant parasitic nematodes. Les nématodes parasites de plantes sont connus pour provoquer de sévères réductions dans les rendements des cultures. Actuellement, un effort se développe pour créer de nouveaux cultivars résistants aux nématodes. Les nématodes parasite de plantes appartiennent à trois ordres très éloignés: Triplonchida, Dorylaimida et Tylenchida. Tous les nématodes parasites de plantes chez les Triplonchida et Dorylaimida sont des ectoparasites migrateurs. Chez les Tylenchida, plusieurs types différents de parasitisme peuvent être identifiés. Les endoparasites sédentaires ont l’interaction la plus complexe avec leur hôte et sont responsables de la plus grande part des dégâts agricoles. C’est la raison pour laquelle la plupart des recherches sont concentrées sur deux groupes de nématodes endoparasites sédentaires, les nématodes à kystes et les nématodes galligènes. Ces deux groupes induisent des structures d’alimentation spécialisées dans les tissus vasculaires de la racine végétale. Le mécanisme parasitaire des nématodes à kystes et galligènes est revu, certaines de leurs caractéristiques pouvant être applicables à l’étude des autres nématodes phytoparasites.

Root-Knot Nematode Management in Caladium

EDIS ◽

10.32473/edis-in1325-2021 ◽

2021 ◽

Vol 2021 (4) ◽

Author(s):

Mengyi Gu ◽

Johan Desaeger

Keyword(s):

Management Strategies ◽

Commercial Production ◽

Parasitic Nematodes ◽

Root Knot Nematode ◽

Root Knot Nematodes ◽

Nematode Management ◽

Florida leads the commercial production of caladiums, a popular ornamental. In Florida, root-knot, sting, and stubby root nematodes are found in caladium fields. Root-knot nematodes are considered the most important of all pests in caladiums that are grown in sand. This publication will help caladium growers understand what plant-parasitic nematodes are and current nematode management strategies for the caladium industry.

Parasitic nematodes of wild and cultivated subtropical fruit plants in Central Asia

Russian Journal of Parasitology ◽

10.31016/1998-8435-2021-15-1-98-102 ◽

2021 ◽

Vol 15 (1) ◽

pp. 98-102

Author(s):

Sh. Kh. Khurramov ◽

A. S. Bekmuradov

Keyword(s):

Central Asia ◽

Parasitic Nematodes ◽

Fruit Crops ◽

Root Knot Nematodes ◽

Central Asian ◽

Fruit Plants ◽

Subtropical Fruit ◽

Central Asian Republics ◽

The purpose of the research is studying the species composition and control measures against parasitic nematodes of wild and cultivated subtropical fruit plants of Central Asia.Materials and methods. We studied subtropical fruit crops in 198 farms and 1985 household plots located in various soil and climatic zones of over 20,675 hectares in Central Asia. The materials were collected in the autumn (September-October), spring (April-May) and summer (June-August) months in 1970–1990 by the route method. Study subjects were plant parasitic nematodes of 8 species of wild and cultivated subtropical fruit plants in the Central Asian republics. A modified Baermann funnel method was used to isolate plant nematodes from plants and soil near roots. In total, we collected and analyzed more than 5,400 plant and soil samples. To identify the species of plant parasitic nematodes, we used the Atlas of plant parasitic nematodes compiled at the Institute of Parasitology of the Russian Academy of Sciences, as well as morphometric indicators obtained according to the generally accepted De Mann formulae.Results and discussion. More than 129,000 specimens of plant parasites of 98 species were found in the studied wild and cultivated subtropical fruit crops and soil near their roots. In many farms of the Central Asian republics, we found southern root-knot nematodes, peanut root-knot nematodes, javanese root-knot nematodes, and cotton root-knot nematodes in mixed populations. In Uzbekistan, the prevalence in these crops was from 8 to 61.3%, in Tajikistan – from 78 to 98%, in Turkmenistan – from 6 to 98%, and in Kyrgyzstan – 33%. In addition to root-knot nematodes, representatives of ecto- and endoparasites of the genera Tylenchorhynchus, Merlinius, Quinisulcius, Rotylenchus, Helicotylenchus, Pratylenchus, Paratylenchus, Macroposthonia, Labocriconema and Xiphinema were parasitizing in the above subtropical cultures. Under production conditions, we tested Furadan at a dose of 40 kg/ha, Heterophos at a dose of 60 and 120 kg/ha, and Aldicarb at a dose of 40 kg/ha to control root-knot and other parasitic nematodes. Their efficacy ranged from 89 to 100%. We also used the soil solarization method developed by us, the efficacy of which was 95–98%.

Distribution of plant parasitic nematodes in irrigated vineyards at Merbein and Robinvale

Australian Journal of Experimental Agriculture ◽

10.1071/ea9620008 ◽

1962 ◽

Vol 2 (4) ◽

pp. 8 ◽

Cited By ~ 4

Author(s):

MR Sauer

Keyword(s):

Parasitic Nematodes ◽

Root Knot Nematodes ◽

Irrigation Districts ◽

Citrus Nematode ◽

Surveys made through the irrigation districts of Merbein and Robinvale, settlements established at different times on similar soils, showed that several genera of plant parasitic nematodes were widely associated with growing of grape vines. These genera included Meloidogyne, Tylenchulus, Pratylenchus, Paratylenchus and Criconemoides. Meloidogyne was more widely distributed in the newer area of Robinvale (37 per cent of samples infested) than in the older area of Merbein (22 per cent infested). This is most likely due to increasing infestation of vine numerics. In one com- paratively new section of Merbein where vegetable growing among vines has been common practice, half the samples contained Meloidogyne larvae. Root knot nematodes were most common in light textured soils. Over 90 per cent of samples from Merbein contained Tylenchulus, probably became citrus is grown or has been grown on very many properties. At Robinvale a little over 20 per cent of samples contained citrus nematode. Plant parasitic nematodes are sufficiently common at the present time in the irrigation areas to constitute an important problem in grape production.