The pelleting process or treatment with sodium hydroxide do not detoxify the toxins of annual ryegrass toxicity.

Author(s):  
J. G. Allen ◽  
M. Hoxey ◽  
C. Thompson ◽  
A. M. Masters ◽  
J. T. B. Milton ◽  
...  
2006 ◽  
Vol 57 (7) ◽  
pp. 731 ◽  
Author(s):  
A. M. Masters ◽  
A. R. Gregory ◽  
R. J. Evans ◽  
J. E. Speijers ◽  
S. S. Sutherland

An enzyme-linked immunosorbent assay (ELISA) for Rathayibacter toxicus is described. The development of a monoclonal antibody for a specific antigen from R. toxicus and a polyclonal antibody raised against the same R. toxicus preparation enabled a capture assay format. The assay is specific for a soluble polysaccharide produced by the bacterium and was found to be sensitive enough to detect antigen equivalent to less than one gall per kilogram of hay. The applicability of the assay to samples of pasture or hay is demonstrated. Cost-effective testing of large numbers of samples for the presence of R. toxicus is possible with the ELISA. This will assist stockowners, hay producers, and hay exporters in the management of the risk of annual ryegrass toxicity.


1994 ◽  
Vol 45 (4) ◽  
pp. 841 ◽  
Author(s):  
IT Riley

Biological factors contributing to the decline in the incidence of annual ryegrass toxicity (ARGT) in Western Australia were investigated. Annual ryegrass and soil samples were collected in the area where ARGT outbreaks first occurred in Western Australia, an area where the incidence of ARGT has markedly declined. The nematode vector, Anguina funesta, was found in 68% of samples, whereas the toxigenic bacterium, Clavibacter toxicus, was detected in fewer than 2% of samples. The population densities of the nematode were low, but other attributes assayed were normal. The fungus, Dilophospora alopecuri, also associated with A. funesta, was found at 58% of the sampling sites. The abundance of D. alopecuri suggests that it is an important contributor to decline in ARGT. Therefore, the distribution of D. alopecuri in Western Australia was determined in the following season. D. alopecuri was common in the southerly range of A. funesta, but absent in the northerly range where ARGT outbreaks are still common. In the south, D. alopecuri appears to have displaced C. toxicus and reduced A. funesta populations. The findings suggest that there is potential to use D. alopecuri to control ARGT


1983 ◽  
Vol 34 (4) ◽  
pp. 403 ◽  
Author(s):  
AC McKay ◽  
JM Fisher ◽  
AJ Dube

To control the nematode Anguina funesta (considered by some to be A. agrostis), the vector in annual ryegrass toxicity, pasture management treatments were applied before and after gall initiation. Before gall initiation, the herbicides paraquat and diclofop methyl were used to remove ryegrass. These treatments gave good control of the nematode, but reduced pasture production in winter when feed is generally scarce. Diclofop methyl had no detrimental effect on legume growth, and was more effective on actively growing ryegrass than was paraquat, which severely retarded subterranean clover. Desiccating the ryegrass heads, with mechanical topping or the desiccant herbicide paraquat, after gall initiation, but before hatching of the second-stage juveniles ( J2s ) , gave good control of the nematode population. The main limitations with mechanical topping are that the ryegrass heads must be tall enough to cut before the J2s hatch and not all paddocks are suitable for topping. With paraquat, the main limitations are that treated material was susceptible to weathering and there was less regrowth to set seed. Oxamyl, a systemic nematicide applied after gall initiation, had no effect on the nematode, nor had spraying pasture with the nematode parasitic fungus Verticillium sp.


1996 ◽  
Vol 117 (2) ◽  
pp. 393-400 ◽  
Author(s):  
M. S. Johnston ◽  
S. S. Sutherland ◽  
C. C. Constantine ◽  
D. J. Hampsons

SummaryMultilocus enzyme electrophoresis was used to examine the relatedness of 52 isolates ofClavibacter toxicus, the agent of annual ryegrass toxicity. These included 37 Western Australian (WA) field isolates sampled in 3 distinct locations over a 2-year period, and 15 isolates sampled from 6 different host plant species in 3 states in Australia over approximately 8 years. Seventeen reference strains for the related generaCurtobacterium, RhodococcusandArthrobacterwere examined for comparison. The 69 isolates were divided into 29 electrophoretic types (ETs), separated by genetic distances of 0·06 to 0·81. TheC. toxicusisolates fell into 12 ETs, 11 of which formed a tightly clustered group separated by a genetic distance of 0·23 or less. Thirty-one of the WA field isolates ofC. toxicusfell into a single ET, and four into another ET.Clavibacter toxicustherefore formed a closely related group which was genetically distinct from the other plant pathogenic species, and a dominant widely disseminated strain of the species was identified in WA.


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