scholarly journals The effect of supplements of groundnut flour or groundnut protein isolate fortified with calcium salts and vitamins or of skim-milk powder on the digestibility coefficient, biological value and net utilization of the proteins of poor Indian diets given to undernourished children

1963 ◽  
Vol 17 (1) ◽  
pp. 13-18
Author(s):  
Kantha Joseph ◽  
P. K. Tasker ◽  
M. Narayanarao ◽  
M. Swaminathan ◽  
A. Sreenivasan ◽  
...  
Keyword(s):  
Milk Powder ◽  
Skim Milk ◽  
Skim Milk Powder ◽  
Protein Isolate ◽  
Calcium Salts ◽  
Biological Value

THE EFFECT OF FORTIFICATION OF PROCESSED SOYA FLOUR WITH dl-METHIONINE HYDROXY ANALOGUE OR dl-METHIONINE ON THE DIGESTIBILITY, BIOLOGICAL VALUE, AND NET PROTEIN UTILIZATION OF THE PROTEINS AS STUDIED IN CHILDREN

1964 ◽  
Vol 42 (3) ◽  
pp. 377-384 ◽  
Author(s):  
H. N. Parthasarathy ◽  
T. R. Doraiswamy ◽  
Myna Panemangalore ◽  
M. Narayana Rao ◽  
B. S. Chandrasekhar ◽  
...  
Keyword(s):  
Daily Intake ◽  
Milk Powder ◽  
Skim Milk ◽  
Skim Milk Powder ◽  
Soya Flour ◽  
Protein Utilization ◽  
Biological Value ◽  
True Digestibility ◽  
The Mean ◽  
Net Protein

The true digestibility coefficient, biological value, and net available protein of diets based on processed soya flour supplemented with dl-methionine hydroxy analogue (MHA) or dl-methionine (at a level of 1.2 g/16 g N) have been determined in children aged 8–9 years. The mean daily intake of protein by the children on the different diets was maintained at a level of about 1.2 g/kg body weight. Supplementation of soya flour with dl-methionine brought about a marked increase in the biological value and net protein utilization of the proteins. MHA was, however, slightly less effective than dl-methionine in this respect. The biological value and net protein utilization of the different proteins were as follows: soya flour, 63.5 and 53.3; soya flour + MHA, 71.5 and 61.4; soya flour + methionine, 74.9 and 64.7; and skim milk powder, 82.6 and 72.0.

QUALITY OF PROTEIN IN MILK REPLACERS FOR YOUNG CALVES. IV. RENNET (CHYMOSIN, RENNIN) COAGULATION OF RECONSTITUTED SKIM MILK POWDER CONTAINING ADDED PROTEINS, FAT, CALCIUM, PHOSPHATE AND CITRATE

1976 ◽  
Vol 56 (2) ◽  
pp. 339-345 ◽  
Author(s):  
D. B. EMMONS ◽  
J. D. JONES ◽  
E. E. LISTER
Keyword(s):  
Soy Protein Isolate ◽  
Milk Powder ◽  
Skim Milk ◽  
High Heat ◽  
Skim Milk Powder ◽  
Protein Isolate ◽  
Active Principle ◽  
Total Solids ◽  
Ph Values ◽  
Milk Replacers

The effects of added nutrient sources on curd firmness of reconstituted skim milk powder (10% total solids) were studied. Defatted rapeseed flour (4%) had little effect whereas whey powder (4%) and fish protein concentrate (2%) decreased gel strength of the reconstituted skim milk by approximately 30%. Soybean meal (4%) and soy protein isolate (2%) markedly reduced curd firmness; addition of CaCl2 restored coagulability and curd firmness. However, addition of CaCl2 did not restore gel firmness in reconstituted, severely heated skim milk powder to that of a low-temperature product. Addition of 0.1% CaCl2 slightly increased firmness of reconstituted low- and high-heat powder at pH 6.1; further addition to 0.4% CaCl2 decreased firmness slightly. It appears that when there is sufficient available Ca, further additions have no effect on curd firmness. The results indicate that milk replacers which coagulate with rennet (containing chymosin or rennin as active principle) can probably be produced even when some of the skim milk protein is replaced by other less expensive proteins. Addition of citrate (14 mM), followed by adjustment of the pH, prevented coagulation at pH 6.1 and markedly reduced firmness at pH 5.6 and 5.2. Addition of citrate at 5.7 mM did not prevent coagulation at those pH values. Addition of phosphate had little effect on curd firmness at these pH values. Addition of fat to skim milk containing 15 or 20% total solids, followed by homogenization, had little effect on curd firmness at pH 6.1. Fat additions, however, decreased curd firmness of skim milk containing 10% total solids and nearly prevented coagulation in skim milk containing 5% total solids.

Use of an infant food formula based on groundnut protein isolate and skim milk powder in feeding infants

1963 ◽  
Vol 30 (11) ◽  
pp. 365-368 ◽  
Author(s):  
T. R. Doraiswamy ◽  
M. R. Chandrasekhara ◽  
B. H. Subbaraya ◽  
A. N. Sankaran ◽  
M. Swaminathan ◽  
...  
Keyword(s):  
Milk Powder ◽  
Skim Milk ◽  
Skim Milk Powder ◽  
Protein Isolate ◽  
Infant Food

THE EFFECT OF SUPPLEMENTING A RICE DIET WITH LYSINE, METHIONINE, AND THREONINE ON THE DIGESTIBILITY COEFFICIENT, BIOLOGICAL VALUE, AND NET PROTEIN UTILIZATION OF THE PROTEINS AND ON THE RETENTION OF NITROGEN IN CHILDREN

1964 ◽  
Vol 42 (3) ◽  
pp. 385-393 ◽  
Author(s):  
H. N. Parthasarathy ◽  
Kantha Joseph ◽  
V. A. Daniel ◽  
T. R. Doraiswamy ◽  
A. N. Sankaran ◽  
...  
Keyword(s):  
Body Weight ◽  
Milk Powder ◽  
Skim Milk ◽  
Skim Milk Powder ◽  
Protein Utilization ◽  
N Retention ◽  
Biological Value ◽  
True Digestibility ◽  
Net Protein ◽  
Rice Diet

The effect of supplementing a rice diet providing about 1.3–1.4 g protein per kg body weight with lysine, methionine, and threonine individually or together on true digestibility coefficient (DC), biological value (BV), and net protein utilization (NPU) of the proteins has been studied in girls aged 8–9 years. The retention of nitrogen on the rice diet was very low (9.5% of intake in the first series and 8.5% in the second series). The BV and NPU of the proteins of rice diet were 64.1 and 52.9 in the first series and 66.6 and 54.9 in the second series. Supplementation of the rice diet with lysine or methionine or lysine + methionine brought about a significant improvement in N retention (12.6, 12.0, 13.5% of intake) and in the BV (68.3, 66.3, 69.3) and NPU (54.8, 55.7, 55.8). When the rice diet was supplemented with lysine and threonine, a highly significant improvement in the N retention (18.9% of intake) and in the BV (77.4) and NPU (63.4) was observed. Addition of methionine to rice diet containing lysine and threonine resulted in a further improvement in N retention, BV, and NPU of the diets. The net available protein (g/kg body weight) from the different diets were as follows: rice diet, 0.71; rice diet + lysine, 0.76; rice diet + methionine, 0.74; rice diet + lysine + methionine, 0.77; rice diet + lysine + threonine, 0.85; rice diet + lysine + threonine + methionine, 0.91; and skim milk powder diet, 0.96–0.98.

THE METABOLISM OF NITROGEN AND THE DIGESTIBILITY COEFFICIENT AND BIOLOGICAL VALUE OF THE PROTEINS AND NET PROTEIN UTILIZATION IN POOR RICE DIET SUPPLEMENTED WITH METHIONINE-FORTIFIED SOYA FLOUR OR SKIM MILK POWDER

1964 ◽  
Vol 42 (5) ◽  
pp. 641-650 ◽  
Author(s):  
Myna Panemangalore ◽  
H. N. Parthasarathy ◽  
Kantha Joseph ◽  
A. N. Sankaran ◽  
M. Narayana Rao ◽  
...  
Keyword(s):  
Milk Powder ◽  
Skim Milk ◽  
Nitrogen Retention ◽  
Skim Milk Powder ◽  
Protein Supplement ◽  
Soya Flour ◽  
Protein Utilization ◽  
Biological Value ◽  
Net Protein ◽  
Rice Diet

The effect of supplementing a poor rice diet commonly used in India with methionine-fortified soya flour or skim milk powder was studied in seven girls aged 8–9 years, using as criteria: the retention of nitrogen, digestibility coefficient, biological value, and net protein utilization of the proteins. The retention of nitrogen on the rice diet was very low (20.3 mg/kg day). Supplementation of the rice diet with soya flour, methionine-fortified soya flour or skim milk powder, so as to provide about 1 g/kg extra protein, made up the protein deficiency in the rice diet and resulted in a significant increase in nitrogen retention (95.6, 112.1, and 113.6 mg/kg day). The retention of nitrogen on the rice diet supplemented with methionine-fortified soya flour (112.1 mg/kg day) or skim milk powder (113.6 mg/kg day) was nearly the same and significantly higher than that (95.6 mg/kg day) observed with a diet supplemented with soya flour. The biological value and net protein utilization of the mixed proteins of rice – skim milk powder diet and rice–methionine-fortified soya flour diet (62.4 and 62.2 and 53.3 and 54.0 respectively) were nearly the same and significantly higher than those (58.3 and 49.9) of rice – soya flour diet. The results show that methionine-fortified soya flour is almost as good as skim milk powder and significantly superior to soya flour as a protein supplement to rice diet.

343. Studies of the Biological Value of the Proteins (Nitrogen) of Dried Skim Milk: Value As An Addition to Bread; Tests With Young and Olderrats; Effects of Storage

1946 ◽  
Vol 14 (3) ◽  
pp. 403-414 ◽  
Author(s):  
Kathleen M. Henry ◽  
S. J. Rowland
Keyword(s):  
Total Nitrogen ◽  
Milk Powder ◽  
Skim Milk ◽  
Skim Milk Powder ◽  
Biological Value ◽  
Young Rats ◽  
A Value ◽  
True Digestibility

1. A sample of roller-dried skim-milk powder, found to possess the very low biological value of 55.2, supplemented slightly but definitely the proteins of national wheatmeal bread when it supplied 23% of the total nitrogen. No such effect was observed with another sample of milk with a biological value of 71.1 when it supplied 17 % of the total nitrogen, or with a further sample of milk with a biological value of 81.3 when added to bread at a 6 % level supplying about 15 % of the total nitrogen. On the first two occasions the biological value for two different samples of national wheatmeal bread was 48.9 and 55.0 respectively for young rats; for the same rats when mature the values were 55.8 and 64.6. No differences attributable to age were found with milk. Baking did not affect the biological value of the proteins of the milk added to the flour.2. The biological value of a sample of spray.dried skim milk declined from a value of 88.5, observed 18 months after manufacture, to 71.1 some 36 months later. The true digestibility remained unchanged.3. Commercial samples of spray.dried and roller.dried skim milk of good quality, and of over.neutralized and overheated roller.dried skim milk, yielded biological values of 78.6, 80.9, 78.2 and 76.2 respectively and true digestibilities of 90.5, 90.1, 89.4 and 88.8 respectively.

scholarly journals Determination of Immunoglobulin G in Bovine Colostrum and Milk Powders, and in Dietary Supplements of Bovine Origin by Protein G Affinity Liquid Chromatography: Collaborative Study

2010 ◽  
Vol 93 (2) ◽  
pp. 622-627 ◽  
Author(s):  
Grant Abernethy ◽  
Don Otter ◽  
K Arnold ◽  
J Austad ◽  
S Christiansen ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Collaborative Study ◽  
Milk Powder ◽  
Skim Milk ◽  
Skim Milk Powder ◽  
Protein Isolate ◽  
Whey Protein Concentrate ◽  
Protein G ◽  
Low Protein ◽  
Dairy Powders

Abstract An AOAC collaborative study was conducted to evaluate an affinity LC procedure for measuring immunoglobulin G (IgG) in selected dairy powders. The powders were extracted with 0.15 M sodium chloride solution and the pH was adjusted to 4.6 to precipitate caseins, which would otherwise lead to an overestimation of IgG. The analyte was then bound to a commercially available Protein G affinity cartridge and selectively eluted with a glycine buffer at pH 2.5. Detection was at 280 nm and quantification was made against a calibration curve prepared from bovine serum IgG. The samples analyzed included the likely matrixes for which this assay will find commercial use, namely, high- and low-protein-content colostrum powders, tablets containing colostrum powder, and some IgG-containing dairy powders; milk protein isolate, whey protein concentrate, and skim milk powder. Eleven laboratories provided data for the study and assayed blind duplicates of six materials. The repeatability RSD values ranged from 2.1 to 4.2 and the reproducibility RSD values ranged from 6.4 to 18.5. The Protein G method with casein removal has adequate reproducibility for measuring IgG in colostrum-derived powders that are traded on the basis of IgG content as a colostral marker.

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