EFFECT OF NITROGEN AND CARBON SOURCES ON THE INORGANIC PHOSPHATE SOLUBILIZATION BY DIFFERENTASPERGILLUS NIGERSTRAINS

2004 ◽  
Vol 191 (8) ◽  
pp. 1043-1052 ◽  
Author(s):  
S. SESHADRI ◽  
S. IGNACIMUTHU ◽  
C. LAKSHMINARASIMHAN
Keyword(s):  
Inorganic Phosphate ◽  
Phosphate Solubilization ◽  
Carbon Sources

scholarly journals Inorganic Phosphate Solubilization by a Novel Isolated Bacterial Strain Enterobacter sp. ITCB-09 and Its Application Potential as Biofertilizer

Agriculture ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 383 ◽  
Author(s):  
Gustavo Enrique Mendoza-Arroyo ◽  
Manuel Jesús Chan-Bacab ◽  
Ruth Noemi Aguila-Ramírez ◽  
Benjamín Otto Ortega-Morales ◽  
René Efraín Canché Solís ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Inorganic Phosphate ◽  
Extracellular Polymeric Substances ◽  
Phosphate Solubilization ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Habanero Pepper ◽  
Phosphate Solubilizing

The excessive use of fertilizers in agriculture is mainly due to the recognized plant requirements for soluble phosphorus. This problem has limited the implementation of sustainable agriculture. A viable alternative is to use phosphate solubilizing soil microorganisms. This work aimed to isolate inorganic phosphorus-solubilizing bacteria from the soils of agroecosystems, to select and identify, based on sequencing and phylogenetic analysis of the 16S rRNA gene, the bacterium with the highest capacity for in vitro solubilization of inorganic phosphate. Additionally, we aimed to determine its primary phosphate solubilizing mechanisms and to evaluate its effect on Habanero pepper seedlings growth. A total of 21 bacterial strains were isolated by their activity on Pikovskaya agar. Of these, strain ITCB-09 exhibited the highest ability to solubilize inorganic phosphate (865.98 µg/mL) through the production of organic acids. This strain produced extracellular polymeric substances and siderophores that have ecological implications for phosphate solubilization. 16S rRNA gene sequence analysis revealed that strain ITCB-09 belongs to the genus Enterobacter. Enterobacter sp. ITCB-09, especially when immobilized in beads, had a positive effect on Capsicum chinense Jacq. seedling growth, indicating its potential as a biofertilizer.

Influence of Salinity and pH on Inorganic Phosphate Solubilization by Bacteria of the Genus Burkholderia

2014 ◽  
Author(s):  
J.A.T. Silva ◽  
C.G.G. Santos ◽  
F.G. Silva ◽  
I.B. Santos ◽  
M.B.G.S. Freire ◽  
...  
Keyword(s):  
Inorganic Phosphate ◽  
Phosphate Solubilization

scholarly journals Crc Regulates Succinate-Mediated Repression of Mineral Phosphate Solubilization in Acinetobacter sp. SK2 by Modulating Membrane Glucose Dehydrogenase

2021 ◽  
Vol 12 ◽  
Author(s):  
Krishna Bharwad ◽  
Niharika Ghoghari ◽  
Shalini Rajkumar
Keyword(s):  
Glucose Oxidation ◽  
Phosphate Solubilization ◽  
Glucose Dehydrogenase ◽  
Carbon Sources ◽  
Shoot Length ◽  
Wild Type ◽  
Mineral Phosphate Solubilization ◽  
Root And Shoot Length ◽  
Mineral Phosphate ◽  
Encoding Gene

The plant growth-promoting Acinetobacter sp. SK2 isolated from Vigna radiata rhizosphere was characterized for mineral phosphate solubilization (MPS). To understand the contribution of the membrane glucose dehydrogenase (mGDH) and soluble glucose dehydrogenase (sGDH) in glucose oxidation and MPS, insertional inactivation of the corresponding genes was carried out. The disruption of mGDH encoding gene gdhA resulted in complete loss of mGDH activity, which confirmed its role in periplasmic glucose oxidation and gluconate-mediated MPS phenotype. The inactivation of sGDH encoding gene gdhB resulted in loss of sGDH activity, which did not alter the MPS or mGDH activity. Thus, it was also concluded that the sGDH was dispensable in gluconate-mediated MPS. Supplementation of succinate in glucose-containing medium suppressed the activity of mGDH (and sGDH) and therefore repressed the MPS phenotype. The catabolite repression control protein (Crc) of Pseudomonas was implicated in Acinetobacter sp. for a similar function in the presence of preferred and non-preferred carbon sources. To understand the regulatory linkage between Crc and genes for glucose oxidation, crc mutants were generated. The inactivation of crc resulted in increased activity of the mGDH in glucose + succinate-grown cells, indicating derepression. An increase in phosphate solubilization up to 44% in glucose + succinate-grown crc– compared with glucose-grown cells was recorded, which was significantly repressed in the wild-type strain under similar conditions. It is therefore proposed that in Acinetobacter sp. SK2, Crc is involved in the succinate-provoked repression of the MPS phenotype. The gene expression data indicated that Hfq may also have a regulating role in preferential utilization of carbon source by perhaps modulating Crc–Hfq functionality. V. radiata plants inoculated with the wild type improved both root and shoot length by 1.3 to 1.4-fold. However, crc– increased the root and shoot length by 1.6-fold, compared with the uninoculated controls. In mimicking the soil condition (in the presence of multiple carbon sources, e.g., succinate along with glucose), the crc– strain of Acinetobacter sp. SK2 performed better in supporting the growth of V. radiata in pot experiments.

Evaluation of Inorganic Phosphate Solubilizing Efficiency and Multiple Plant Growth Promoting Properties of Endophytic Bacteria Isolated From Root Nodules Erythrina Brucei

2021 ◽  
Author(s):  
Belay Berza ◽  
Jegan Sekar ◽  
Prabavathy VR ◽  
Marcela C Pagano ◽  
Fassil Assefa
Keyword(s):  
Bacillus Thuringiensis ◽  
Inorganic Phosphate ◽  
Phosphate Solubilization ◽  
Root Nodules ◽  
Achromobacter Xylosoxidans ◽  
Bacterial Endophytes ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing ◽  
Acinetobacter Soli

Abstract Background: The majority of phosphorous in the soil is fixed and unavailable to plant nutrition, hence in scarcity. Phosphate solubilizing bacteria, the ecological engineers, are considered as the best, sustainable and eco-friendly options. The objectives of this study were to screen and evaluate inorganic phosphate solubilizing efficiency and assess multiple plant growth promoting traits of E. brucei root nodule bacterial endophytes.Results: A total of 304 nodule bacterial endophytes were screened for phosphate solubilization potential on solid PA medium among which 119 (39%) were potential tricalcium phosphate solubilizers. None of these isolates were able to form clearly visible halos on aluminum phosphate (AlPO4), Al-P or iron phosphate (FePO4), Fe-P supplemented PA medium. Out of 119 inorganic phosphate solubilizing endophytes, 40.3% were IAA producers. Based on phosphate solubilization index, the potential bacterial endophytes were identified to Gluconobacter cerinus, Acinetobacter soli, Achromobacter xylosoxidans and Bacillus thuringiensis using the 16S rRNA gene sequences analysis. All the selected isolates were potential solubilizers of the three inorganic phosphates (Al-P, Fe-P and tricalcium phosphate, TCP) included in liquid NBRIP medium. The highest values of solubilized TCP were recorded by isolates AU4 and RG6 (A. soli), 108.96 mg L-1 and 107.48 mg L-1, respectively at sampling day3 and 120.36 mg L-1 and 112.82 mg L-1, respectively at day 6. The highest values of solubilized Al-P and Fe-P were recorded by isolate RG6, 102.14 mg L-1 and 96.07 mg L-1, respectively at sampling days 3 and 6, respectively. The highest IAA, 313.61µg mL-1 was recorded by isolate DM17 (B. thuringiensis). These selected potential isolates were also HCN, NH3, and hydrolytic enzymes producers. The isolates were also varied in tolerance to eco-physiological stressors and exhibited versatility to carbon and nitrogen substrate utilization. Conclusions: The genera and species Gluconobacter cerinus, Acinetobacter soli, Achromobacter xylosoxidans and Bacillus thuringiensis are the first reports from E .brucei root nodules and Gluconobacter is also the first report to the science as phosphate solubilizer. Isolates AU4 and RG (A. soli) could be potential bio-inoculant candidates for the growth enhancement of the host plant for better agro-forestry practices in acidic and alkaline soils in Ethiopia.

Inorganic phosphate solubilization by rhizosphere bacteria in a Zostera marina community

1982 ◽  
Vol 28 (6) ◽  
pp. 605-610 ◽  
Author(s):  
Pamela A. Craven ◽  
Steven S. Hayasaka
Keyword(s):  
Acetic Acid ◽  
Calcium Phosphate ◽  
Zostera Marina ◽  
Inorganic Phosphate ◽  
Phosphate Solubilization ◽  
Dry Weight ◽  
Rhizosphere Bacteria ◽  
Bacterial Isolates ◽  
Minimum Concentration ◽  
Phosphate Solubilizing

Actively growing Zostera marina plants had a greater rhizosphere inorganic phosphate solubilizing potential than dormant plants. Rhizosphere bacteria that were capable of calcium phosphate solubilization were obligate aerobes and numbered approximately 4 × 108 colony-forming units/g dry weight root. Bacterial isolates solubilized calcium phosphate when cultured with glucose as the sole carbon and energy source but not when cultured with amino acids. Both calcium phosphate (hydroxyapatite) and glucose were found in sea grass bed sediment. Acetic acid was also detected from roots plus clinging sediment, from sediment, and from cultured bacterial isolates in a glucose-supplemented medium. The minimum concentration of acetic acid that showed detectable solubilization of calcium phosphate was 10−5 M. It is suggested that acetic acid, a product of glucose metabolism in the rhizosphere flora, is responsible for phosphate solubilization in the environment.

scholarly journals Effect of Carbon Sources on Different Strains of Phytase-Producing Bacteria Isolated from Malaysia’s Hot Spring

2021 ◽  
Vol 12 (2) ◽  
pp. 29-38
Author(s):  
Nurul Izyan Che Mohamood ◽  
Nadiawati Alias ◽  
Nurul Asma Hasliza Zulkifly
Keyword(s):  
Phytic Acid ◽  
Inorganic Phosphate ◽  
Hot Springs ◽  
Animal Feed ◽  
Hot Spring ◽  
Carbon Sources ◽  
Feed Additives ◽  
Phytase Activity ◽  
Phytase Production ◽  
Different Strains

Animal feed from cereal grains and oilseed meals mainly containing phytic acid which has adverse effects on animal nutrition and its environment. Ruminants can easily digest the phytic acid as they have fungi and bacteria in their guts which can produce phytase to degrade the phytic acid. Meanwhile, phytic acid in non-ruminant animals is poorly digested due to the lack of sufficient phytase in their guts. Thus, the feed must be supplemented with inorganic phosphate to ensure it can absorb adequate nutrients. This study aimed to determine the effects of using different carbon sources to the growth of different strains of phytase producing bacteria based on optical density (OD), colony forming unit (CFU), and their phytase production. All four strains of potentially producing-phytase bacteria  have been isolated from several hot springs in Malaysia. The bacteria were grown in modified Phytase Screening Medium (PSM) with glucose and lactose as a carbon source and under optimum culture conditions (pH 5.5, 37˚C, 200 rpm) for 72 hours. For quantitative screening of phytase production, the bacterial cultures were harvested to obtain the supernatants that were used to measure the amount of inorganic phosphorus released by the bacterial strains. Among these carbon sources, glucose has shown consistency between their CFU counts and the observed ODs whereas lactose shown inconsistency. Meanwhile, the maximum phytase activity was recorded for all strains in the presence of glucose in which bacteria strain L3 (0.0404 U/mL), RT (0.0359 U/mL), B9 (0.0262 U/mL), and A (0.0263 U/mL). As for the overall, strain L3 (Labis, Johor) gave a promising rate of inorganic phosphate released with optimum phytase activity value of 0.0404 U/mL in presence of glucose and lactose. The optimisation of the fermentation medium can contribute to more economical production of industrial enzyme as phytase has the potential to produce feed additives for poultry feeding.

scholarly journals Screening and optimization of indole-3-acetic acid production and phosphate solubilization by rhizobacterial strains isolated from Acacia cyanophylla root nodules and their effects on its plant growth

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Sara Lebrazi ◽  
Karsten Niehaus ◽  
Hanna Bednarz ◽  
Mouhcine Fadil ◽  
Marwa Chraibi ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Inorganic Phosphate ◽  
Phosphate Solubilization ◽  
Crop Yields ◽  
Root Nodules ◽  
Iaa Production ◽  
Acacia Cyanophylla ◽  
Indole 3 Acetic Acid

Abstract Background Plant growth-promoting rhizobacteria (PGPR) are known to improve plant growth and are used as biofertilizers, thanks to their numerous benefits to agriculture such as phosphorus solubilization and phytohormone production. In this paper, four rhizospheric bacteria (Phyllobacterium sp., Bacillus sp., Agrobacterium sp., and Rhizobium sp.) isolated from surface-sterilized root nodules of Acacia cyanophylla were tested for their ability to solubilize inorganic phosphate and to produce indole-3-acetic acid (IAA) under laboratory conditions. Then, the best IAA producer (Rhizobium sp.) was selected to test optimized conditions for IAA production. Finally, the effect of the four strains on plant growth for A. cyanophylla was evaluated in vivo. Results The results showed that the totality of the tested isolates had solubilized inorganic phosphate (P) in both NBRIP (National Botanical Research Institute Phosphate) and PVK (Pikovskaya) media. Bacillus sp. was a high P-solubilizer and showed maximum solubilization in PVK (519 μg ml-1) and NBRIP (782 μg ml-1). The optimization of maximum phosphate solubilization was done using different sources of carbon (1%) and nitrogen (0.1%). Glucose and ammonium sulfate were selected to be the best carbon and nitrogen source for phosphate solubilization by all tested strains, except for Phyllobacterium sp., which recorded the highest phosphate solubilization with ammonium nitrate. The IAA production by the tested strains indicated that Rhizobium sp. produced the highest amount of IAA (90.21 μg ml-1) in culture media supplemented with L-tryptophan. The best production was observed with L-Trp concentration of 0.2% (116.42 μg ml-1) and at an initial pH of 9 (116.07 μg ml-1). The effect of NaCl on IAA production was tested at concentrations of 0 to 5% and the maximum production of  89.43 μg ml-1 was found at 2% NaCl. The extraction of crude IAA from this strain was done and purity was confirmed with Thin Layer Chromatography (TLC) analysis. A specific spot from the extracted IAA production was found to correspond with a standard spot of IAA with the same Rf value. Finally, the tested PGPR demonstrated growth stimulatory effects on Acacia cyanophylla seedlings in vivo, with a great increase of shoots’ and roots’ dry weights, and shoot length compared to control. The rhizobacterial isolates were identified by 16S rDNA sequence analysis as Agrobacterium sp. NA11001, Phyllobacterium sp. C65, Bacillus sp. CS14, and Rhizobium sp. V3E1. Conclusion This study highlights the importance of the use of phosphate solubilizing and IAA producer microorganisms as biofertilizers to increase crop yields. The studied strains showed a significant phosphate solubilization potential and IAA production. The use of selected strains as inoculants would be interesting, in particular with a view of promoting sustainable agriculture. However, further studies to verify the efficacy of the best isolates in situ is certainly required.

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