scholarly journals Effect of Carbon Sources on Different Strains of Phytase-Producing Bacteria Isolated from Malaysia’s Hot Spring

2021 ◽  
Vol 12 (2) ◽  
pp. 29-38
Author(s):  
Nurul Izyan Che Mohamood ◽  
Nadiawati Alias ◽  
Nurul Asma Hasliza Zulkifly
Keyword(s):  
Phytic Acid ◽  
Inorganic Phosphate ◽  
Hot Springs ◽  
Animal Feed ◽  
Hot Spring ◽  
Carbon Sources ◽  
Feed Additives ◽  
Phytase Activity ◽  
Phytase Production ◽  
Different Strains

Animal feed from cereal grains and oilseed meals mainly containing phytic acid which has adverse effects on animal nutrition and its environment. Ruminants can easily digest the phytic acid as they have fungi and bacteria in their guts which can produce phytase to degrade the phytic acid. Meanwhile, phytic acid in non-ruminant animals is poorly digested due to the lack of sufficient phytase in their guts. Thus, the feed must be supplemented with inorganic phosphate to ensure it can absorb adequate nutrients. This study aimed to determine the effects of using different carbon sources to the growth of different strains of phytase producing bacteria based on optical density (OD), colony forming unit (CFU), and their phytase production. All four strains of potentially producing-phytase bacteria  have been isolated from several hot springs in Malaysia. The bacteria were grown in modified Phytase Screening Medium (PSM) with glucose and lactose as a carbon source and under optimum culture conditions (pH 5.5, 37˚C, 200 rpm) for 72 hours. For quantitative screening of phytase production, the bacterial cultures were harvested to obtain the supernatants that were used to measure the amount of inorganic phosphorus released by the bacterial strains. Among these carbon sources, glucose has shown consistency between their CFU counts and the observed ODs whereas lactose shown inconsistency. Meanwhile, the maximum phytase activity was recorded for all strains in the presence of glucose in which bacteria strain L3 (0.0404 U/mL), RT (0.0359 U/mL), B9 (0.0262 U/mL), and A (0.0263 U/mL). As for the overall, strain L3 (Labis, Johor) gave a promising rate of inorganic phosphate released with optimum phytase activity value of 0.0404 U/mL in presence of glucose and lactose. The optimisation of the fermentation medium can contribute to more economical production of industrial enzyme as phytase has the potential to produce feed additives for poultry feeding.

scholarly journals Effect of pH on Different Strains of Phytase Producing Bacteria from Malaysia’s Hot Spring

2020 ◽  
Vol 11 (1S) ◽  
Author(s):  
Ainin Sofiya Kholed ◽  
Nurul Asma Hasliza Zulkifly ◽  
Afnani Alwi@Ali ◽  
Tajul Afif Abdullah ◽  
Nadiawati Alias
Keyword(s):  
Culture Condition ◽  
Hot Spring ◽  
Feed Additives ◽  
Phytase Activity ◽  
Future Research ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Phytase Production ◽  
Sodium Phytate ◽  
The Media

In the recent research, the optimisation of culture condition for phytase production rarely done for Acetinobacter baumanii. The optimisation of the phytase production from the bacterial strains largely contributed by Bacillus sp. The study on the phytase originated from hot spring are limited and the species that identified from the hot spring samples are not in the same species from the previous study and mainly the species isolated from Bacillus sp. In this study, four potential strains of bacteria producing phytase isolated from hot spring in several regions in Malaysia. For enrichment of the bacterial, Nutrient Agar was used, meanwhile for batch culture optimisation, the bacteria producing phytase grown in modified liquid Phytase Screening Media with soy extract as agro residual substrate as a replacement for sodium phytate, the chemical substrate. The bacteria were screened for their ability to produce clear zone in solid PSM with sodium phytate as substrate. Optimisation of media through its physical factor that is pH of the media carried out using shake flask scale in laboratory. The growth of the bacterial strains and phytase activity measured quantitatively through the two different pH of media at pH 5.5 and pH 7. The analysis of colony-forming unit and pH determination after fermentation was carried out in this study. From the study, bacterial strain L3 from Labis, Johor has the highest phytase activity in the two parameters studied where the inorganic phosphate released at pH 5.5 (0.21953 U/mL) and pH 7 (0.2047 U/mL). Optimisation carried out through manipulating the culture condition that is pH of the media to determine at which condition has the highest phytase production. Several effects on enzyme activity caused by culture conditions identified. The optimisation of the fermentation medium able to contribute to the less cost production of the industrial enzyme as phytase has potential production for feed additives for poultry feeding. In the future research, molecular identification of the bacterial strains from the better-quality bacteria producing phytase grown in optimised culture media to investigate the molecular identity of the bacterial.

scholarly journals Comparison on Effects of Temperature on Different Strains of Phytase Producing Bacteria Isolated from Malaysia’s Hot Spring

2020 ◽  
Vol 11 (1S) ◽  
Author(s):  
Anis Adilah Mustafa ◽  
Nurul Asma Hasliza Zulkifly ◽  
Afnani Alwi@Ali ◽  
Tajul Afif Abdullah ◽  
Nadiawati Alias
Keyword(s):  
Enzyme Activity ◽  
Hot Springs ◽  
Ph Value ◽  
Hot Spring ◽  
Culture Method ◽  
Phytase Activity ◽  
Bacterial Strains ◽  
Temperature Optimization ◽  
Biochemical Research ◽  
Different Strains

The main purpose of this research was to find the best growth curve for bacterial growth and the optimum temperature for the production of phytase from different potential phytase producing bacterial strains. A total of four strains used were originally isolated from hot springs in Malaysia, which were in Labis, Johor (L3), Dusun Tua, Selangor (RT), Ulu Legong, Kedah (A) and Ranau, Sabah (B9). Nutrient Agar (NA) and modified Phytase Screening Medium (PSM) liquid media were used for the culture enrichment while optimisation was carried out through batch culture method using a shake-flask scale. Strains growth and enzyme activity were quantitatively measured at different temperatures at (30°C and 37°C) values. Enzyme activity was determined according to the reaction of the phytase with its substrate (sodium phytate) and expressed in units of phytase activity (U/ mL). As for the overall, strain L3 (from Labis, Johor) exhibit promising rate of Pi released in the media at 30°C and 37°C, with optimum phytase activity values of 0.2047 U/mL and 0.2195 U/mL, respectively. The pH of the cultures was also measured, where it shows that strains grown in cultures at 37°C produced a higher phytase activity and resulting a lower reading of pH compared when grown at 30°C. All around, L3 strains has the lowest value of pH when cultured at 30°C and 37°C, with the pH value of 3.62 and 2.37, respectively.  From the result obtained, the lower pH indicates the process of phytic acid degradation take place by the phytase in producing inorganic phosphate (Pi) due to the accumulation of organic acid. Since these bacterial strains were originally taken from Hot Springs, further analysis of temperature optimization using 55°C and even 60°C should be carried out. In the future, biochemical research and molecular identification may also be carried out to identify molecular identity in the strains.

scholarly journals Pengaruh Variasi Media terhadap Aktivitas Fitase Burkholderia Sp. Strain HF. 7

bionature ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Hafsan Hafsan ◽  
Muhammad Maslan ◽  
Mashuri Masri ◽  
Laily Agustina ◽  
Asmuddin Natsir ◽  
...  
Keyword(s):  
Phytic Acid ◽  
Rice Bran ◽  
Growth Phase ◽  
Animal Feed ◽  
Phytase Activity ◽  
Cereal Crops ◽  
Phytase Production ◽  
Descriptive Approach ◽  
Current Value ◽  
Low Activity

Abstract.  Cereals and legumes used as animal feed containing phytic acid which cannot be absorbed by the digestive tract of monogastric animals because phytic acid (C6H18O24P6) is an antinutrient that binds approximately 80% P in feed, also binds to proteins, vitamins and minerals (Mg++, Fe++, Zn++, Mn++, Ca++). Then, one option to overcome this problem is the application of phytase enzymes from various sources, including those produced by bacteria. Phytase enzymes can hydrolyse phytic acid in the feed. This study was aimed to determine the growth phase of Burkholderia sp. HF.7 and optimisation of production of phytase activity from variations of Phytate Production Media (PPM) media. This research used as a descriptive approach. The study design used a completely randomised design (CRD) with a factorial pattern consisting of 2 factors, each variation in phytate sources: calcium (Ca) phytate, rice bran, corn bran, and soybeans. Nitrogen source: (NH4) 2SO4, yeast extract, and peptone. Burkholderia sp. HF.7 Growth phase as a standard for phytase production is the 62-hour stationary phase with an OD value of 2.060 log/cell. The optimum phytase production in the variation of PPM media is soybean-peptone with a protein content value of 46.5 mg/mL and an activity value of 8.20 U/mL under conditions of pH 7 with incubation of 37oC for 62 hours. So, phytase activity produced by PPM of cereal crops has a higher current value compared to PPM Ca-phytate media with low activity value.Keywords: Burkholderia, Phytate, Phytase, enzymes.

scholarly journals Seleksi dan Optimasi Karakter Fisik Bakteri Penghasil Fitase yang Diisolasi dari Sumber Air Panas di Guci, Tegal

2017 ◽  
Vol 14 (1) ◽  
pp. 92
Author(s):  
Catur Suci Purwati ◽  
Sajidan Sajidan ◽  
Adi Ratriyanto ◽  
Adi Magna Patriadi Nuhriawangsa
Keyword(s):  
Phytic Acid ◽  
Incubation Time ◽  
Substrate Concentration ◽  
Hot Springs ◽  
Hot Spring ◽  
Relative Activity ◽  
Extracellular Phytase ◽  
Metal Cofactor ◽  
The Difference ◽  
Acid Substrate

<p>The aimed of this study to isolate, select and characterize physically phytase-producing bacteria<br />from hot springs in the Guci, Tegal. Isolation and enrichment of bacteria using Lurya Betani media<br />with 2% of phytic acid substrate. Extraction of extracellular phytase thus obtained extract coarse and<br />optimization by loking at the relative activity at the difference in the resulting product. Phytaseproducing<br />bacteria can be isolated from the hot springs of the Guci, Tegal with the highest relative<br />activity in AG2 and AG2-1 colonies. The highest relative activity at a temperature of 55°C, pH 6, the<br />incubation time of 90 minutes, the substrate concentration of 3% and a metal cofactor Ca2+ at a<br />concentration of 10-4M. Phytase-producing bacteria can be isolated from a hot spring in Guci, Tegal<br />and have certain physical characteristics.</p>

scholarly journals Effects of Nitrogen Sources in Phytase Production on Bacterial Strains Isolated from Malaysia’s Hot Spring

2021 ◽  
Vol 12 (1S) ◽  
pp. 31-39
Author(s):  
Nur Dinie Zailan ◽  
Nurul Asma Hasliza Zulkifly ◽  
Afnani Alwi ◽  
Siti Noor Syuhada Muhammad Amin ◽  
Nadiawati Alias
Keyword(s):  
Nitrogen Source ◽  
Yeast Extract ◽  
Hot Springs ◽  
Hot Spring ◽  
Inositol Phosphates ◽  
Nitrogen Sources ◽  
Bacterial Strains ◽  
Phytase Production ◽  
Ph Values ◽  
Soybean Extract

Efficient strategies for phytase production gained increasing importance as more applications require high amounts of phytase for the market. Four phytase-producing bacterial strains isolated from Malaysia’s hot springs were used in this study to determine the effect of nitrogen sources on phytase production. All of the strains were screened out by applying halozone method which shows all of the strains were definitely positive phytase producer. Phytase Screening Medium (PSM) with soybean extract as substrate was used as a cultivation medium. Optimised condition with 1.0 % (w/v) of glucose (as carbon source), pH 5.5 and 37°C temperature was applied. Yeast extract and peptone were used to identify optimum nitrogen source in maximum phytase production. Quantitative analysis observed were optical density, colony forming unit, pH values and phytase activity to identify the effect of nitrogen source in phytase production. The finding was bacterial strain L3 as the best producer in producing maximum phytase (0.2162 U/mL) with optimised condition using yeast extract as nitrogen source. Findings in this study proved that yeast extract act as the optimum nitrogen source which contribute to maximum phytase production as supported by previous studies. This study can provide an efficient strategy to produce maximum phytase as few studies stated that phytase is an application tool in functional food production that consists of myo-inositol phosphates that is believed to have important pharmacological effects.

scholarly journals SCREENING OF THERMOPHYLIC MICROORGANISM FROM IJEN CRATER BANYUWANGI AS PHYTASE ENZYME PRODUCER

2010 ◽  
Vol 9 (3) ◽  
pp. 470-473
Author(s):  
Aline Puspita Kusumadjaja ◽  
Tutuk Budiati ◽  
Ni Nyoman Tri Puspaningsih ◽  
Sajidan Sajidan
Keyword(s):  
Phytic Acid ◽  
Hot Spring ◽  
Enzymatic Reaction ◽  
Thermostable Enzyme ◽  
Spring Water ◽  
Phytase Activity ◽  
Cell Type ◽  
Thermophilic Microorganism ◽  
Feed Industry ◽  
Industry Needs

Phytase is enzyme which hydrolysis phytic acid to anorganic phosphate and myo-inositol pentakis-, tetrakis-, tris-, bis-, and monophosphate. The use of phytase in feed industry can overcome environment and nutrition problems which were arisen from unmetabolism phytic acid or its salt by poultry, swine and fish. The feed industry needs a thermostable enzyme due to the need of high temperature in pelleting process, i.e. 81 °C. By using thermostabile phytase, the pelleting process will not affect the enzyme activity. Thermostabile phytase can be isolated from microorganism live in hot spring water or volcano crater. In this study, the screening of thermophylic microorganism having thermostabile phytase activity in Ijen Crater, Banyuwangi, has been done. From this process, it was obtained 33 isolates that produce phytase enzyme. Isolate was code by AP-17 yields highest phytase activity, that is 0.0296 U/mL, so this isolate was choosen for further study. The activity of crude phytase enzyme was measured based on the amount of anorganic phosphate that was produced in enzymatic reaction using UV-VIS spectrophotometer at 392 nm. Based on morphology test to identify the gram type of microorganism, isolate AP-17 has a bacill cell type and identified as positive gram bacteria. This isolate was assumed as Bacillus type.   Keywords: Phytase, thermophilic microorganism, phytase activity

scholarly journals Endophytic bacteria isolated from Phaseolus vulgaris produce phytases with potential for biotechnology application

2018 ◽  
Vol 5 (11) ◽  
pp. 657-671 ◽  
Author(s):  
Leonardo Emanuel de Oliveira Costa ◽  
Thamy Lívia Ribeiro Corrêa ◽  
Janaina Aparecida Teixeira ◽  
Elza Fernandes de Araújo ◽  
Marisa Vieira de Queiroz
Keyword(s):  
Phaseolus Vulgaris ◽  
Phytic Acid ◽  
Endophytic Bacteria ◽  
Animal Feed ◽  
Divalent Cations ◽  
Inorganic Phosphorus ◽  
Divalent Metal Ions ◽  
Feed Supplement ◽  
Phytase Production ◽  
Endophytic Microorganisms

Currently, endophytic microorganisms have become a good source of different enzymes and others metabolites of industrial interest. Among a huge spectral of molecules, enzymes as phytases have been emphasized by the ability to hydrolyze the phytic acid that represents the largest storage form of inorganic phosphorus in cereals, which are the staple diet of monogastric animals such as swine and poultry. Moreover, phytic acid acts as an antinutrient by chelating divalent metal ions, and it is interesting provide phytase as an animal feed supplement for those monogastric animals. In the current study, 158 endophytic bacteria isolated from the leaves of three cultivars of Phaseolus vulgaris were assessed for the ability to produce phytase. Among them, four isolates belonging to the Pseudomonas, Stenotrophomonas, Microbacterium and Rhodococcus genera were highlighted, due their phytase production. The phytase produced by Microbacterium foliorum BAC1157 exhibited activity at 70 oC and stability in the presence of divalent cations, indicating that this phytase has a promising use in the animal feed industry. To the authors' knowledge, this is the first report on phytase production by bacteria of the Microbacterium genera.

scholarly journals 115 Functionality of a novel consensus bacterial 6-phytase variant on ileal phytate degradation in weaned piglets fed diets without inorganic phosphate

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 104-105
Author(s):  
Rachael Hardy ◽  
Yueming Dersjant-Li ◽  
Trine Christensen ◽  
Deepak Velayudhan ◽  
Janet C Remus
Keyword(s):  
Phytic Acid ◽  
Inorganic Phosphate ◽  
Animal Feed ◽  
Body Weight Gain ◽  
Block Design ◽  
Control Diet ◽  
Dependent Manner ◽  
Feed Conversion ◽  
Large White ◽  
Treatment Comparison

Abstract Microbial phytases are used in animal feed to hydrolyze phytic acid (myo-inositol hexakisphosphate, IP6) and phytate (salt of phytic acid), to improve phosphorus (P) bioavailability. This study evaluated the efficacy of a novel consensus bacterial 6-phytase variant (PhyG) on ileal IP6 degradation in piglets. A positive control diet was formulated based on corn and soybean meal with adequate digestible P (2.9g/kg) and calcium (Ca, 7.0g/kg). A negative control (NC) diet was formulated without inorganic P (1.1 g digestible P/kg), reduced in Ca (5.0 g/kg) with analyzed phytate P of 2.6g/kg. This diet was supplemented with PhyG at 250, 500 and 1,000 FTU/kg. A total of 90 crossed Pietrain x (large White x Landrace) 21-d-old piglets were fed adaptation diets until 42 d old and then assigned to pens (2 pigs/pen and 9 pens/treatment) in a completely randomized block design. Piglets were fed mash diets ad libitum for 28 d, at the end of the study 1 piglet/pen was euthanized for collecting ileal digesta samples for determination of IP6 digestibility, using TiO2 as a marker. Data was analyzed by one-way ANOVA using JMP 14.0 with treatment comparison by Tukey test and dose response by curve fitting. The ileal IP6 content was reduced with increasing phytase dose in a dose dependent manner (Plinear &lt; 0.0001; Pexponential = 0.08). Similarly, the ileal IP6 digestibility increased exponentially (P &lt; 0.05) with increasing phytase dose (49.1% for NC and 89.3% for PhyG at 1,000 FTU/kg). Improved ileal IP6 digestibility is closely related to improved bone ash, body weight gain and feed conversion ratio (R2 &gt; 0.91). The in vitro IP6 degradation profile supports these in vivo observations. The results demonstrated that the novel consensus phytase variant is highly efficient in hydrolyzing phytate and increasing the bio-availability of phytate bound P in piglets fed diets without inorganic phosphate.

scholarly journals Isolation and optimization of the growth conditions of thermophilic microorganism from hot springs

2018 ◽  
Vol 17 (03) ◽  
pp. 55-60
Author(s):  
Tri H. Nguyen
Keyword(s):  
Carbon Dioxide ◽  
Hot Springs ◽  
Hot Spring ◽  
Carbon Sources ◽  
Basal Medium ◽  
Growth Conditions ◽  
High Similarity ◽  
Thermophilic Microorganism ◽  
Effects Of Temperature ◽  
Morphology Characteristics

The aim of this study was to isolate and optimize the growth conditions of thermophilic microorganism from hot springs. The isolation was conducted by using the mineral salt basal medium supplemented with 0.6% yeast extract at 50oC. Totally, 33 isolates of thermophilic microorganism were isolated from hot springs at Truong Xuan (Khanh Hoa province) and Binh Chau (Ba Ria - Vung Tau province). The effects of temperature (45 - 80oC), pH (pH 6 - 9) and carbon sources (malate, pyruvate, acetate, glucose, fructose, or carbon dioxide) on the growth of isolates were examined. In addition, the isolate morphology was also investigated by Gram and spore staining. The isolated thermophilic microorganism showed the diversity in colony morphology and color appearance. Most of them were rod shaped, spore-forming and most grew well at 50oC and pH 7. The highest growth of all isolates was observed under malate, glucose, or fructose, as an organic carbon source and unable to use carbon dioxide. Six out of 33 thermophilic microorganism isolates (namely BM7, BS5, NS1, NS3, NS4, and NW6) grew rapidly under high temperatures from 50 - 55oC and their morphology characteristics showed high similarity to Bacillus sp. The study evidenced the polymorphic diversity of thermophiles in the geothermal hot spring ecosystems.

scholarly journals Isolation and Characterization of Phytase from Chicken Manure Bacteria

2015 ◽  
Vol 15 (2) ◽  
pp. 99
Author(s):  
Mega Pratiwi Irawan ◽  
Zeily Nurachman
Keyword(s):  
Phytic Acid ◽  
Animal Feed ◽  
Specific Activity ◽  
Phytase Activity ◽  
Chicken Manure ◽  
Tree Analysis ◽  
Isolation And Characterization ◽  
Extracellular Phytase

Cereals in animal feed contain anti-nutrients of phytic acid that has capability of chelating proteins and cations. Phytasecan be employed to reduce phytic acid through hydrolyzing phytic acid into free phosphate group and lower derivate ofinositol phosphate. The aim of the study was to isolate and characterize phytase obtained from chicken manure bacteria.The study included the screening of phytase-producing bacteria from chicken manure, the homology analysis of bacterium,and the determination of phytase activity. Phytase activity was measured from concentration of free phosphate. The resultsshowed that one phytase-producing isolate obtained from chicken manure grew in the medium containing 5% rice branextract at 37°C for 5 d. Based on a phylogeny is tree analysis of the genes related to 16S rRNA, the isolate was identified asAcinetobacter sp. TZ1. The extracellular phytase expressed by Acinetobacter sp. TZ1 exhibited optimum reactions at pH5 and 50°C. The enzyme showed activity of 64,6 nmol mL–1min–1 and specific activity of 236 nmol min–1mg–1. Relativemolecular mass of phytase TZ1 was ~35 kDa. Phytase obtained is potential to improve animal feed quality by hydrolyzingphytic acid.

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