Doubled haploid wheat lines with high molecular weight glutenin alleles derived from microspore cultures

2017 ◽  
Vol 46 (3) ◽  
pp. 198-211
Author(s):  
Mohamed N. Barakat ◽  
Abdullah A. Al-Doss ◽  
Abdelhalim I. Ghazy ◽  
Khaled A. Moustafa ◽  
Adel A. Elshafei ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Doubled Haploid ◽  
Wheat Lines

Evaluation of baking properties and gluten protein composition of field grown transgenic wheat lines expressing high molecular weight glutenin gene 1Ax1

2001 ◽  
Vol 158 (4) ◽  
pp. 521-528 ◽  
Author(s):  
Indra K. Vasil ◽  
Scott Bean ◽  
Jianmin Zhao ◽  
Patrick McCluskey ◽  
George Lookhart ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Protein Composition ◽  
Transgenic Wheat ◽  
Gluten Protein ◽  
Wheat Lines

scholarly journals Grain quality indices in common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis.

2021 ◽  
Vol 29 ◽  
pp. 87-91
Author(s):  
N. A. Kozub ◽  
I.O. Sozinov ◽  
H.Ya. Bidnyk ◽  
N.O. Demianova ◽  
O.I. Sozinova ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Protein Content ◽  
High Molecular Weight ◽  
Common Wheat ◽  
Grain Quality ◽  
Grain Protein Content ◽  
Grain Protein ◽  
Quality Indices ◽  
Sedimentation Volume ◽  
Wheat Lines

Aim. The aim of this study was to analyze grain quality indices in winter common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis. marked by storage protein loci. Methods. Acid polyacrylamide gel electrophoresis and SDS-electrophoresis of storage proteins were performed to identify introgressions. Grain quality indices (SDS sedimentation volume and grain protein content) were analyzed in lines with introgressed chromosome 1U or its arm 1UL, as well as in the cultivars Panna and Bezostaya 1. Results. SDS-sedimentation volume in the cultivars and lines depended on year’s conditions. The studying of the lines during two years has demonstrated that the presence of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis was associated with a high volume of SDS-sedimentation SDS30 (higher than that in the cultivar Bezostaya 1). The introgressive lines show high grain protein content. Conclusions. The effect of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis on SDS-sedimentation value is similar to that of the high-quality allele Glu-B1al. The lines with the introgressed allele at Glu-U1 from Ae. biuncialis are valuable initial material for breeding for quality.Keywords: Triticum aestivum, Aegilops biuncialis, high molecular weight glutenin subunits, SDS-sedimentation, protein content.

Microtubule motors and other maps

1990 ◽  
Vol 48 (3) ◽  
pp. 1-1
Author(s):  
Richard B. Vallee
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Cytoplasmic Dynein ◽  
Organelle Transport ◽  
Structural Components ◽  
Microtubule Associated Proteins ◽  
Microtubule Motors ◽  
Associated Proteins ◽  
The Subject ◽  
Intracellular Motility

Microtubules are involved in a number of forms of intracellular motility, including mitosis and bidirectional organelle transport. Purified microtubules from brain and other sources contain tubulin and a diversity of microtubule associated proteins (MAPs). Some of the high molecular weight MAPs - MAP 1A, 1B, 2A, and 2B - are long, fibrous molecules that serve as structural components of the cytamatrix. Three MAPs have recently been identified that show microtubule activated ATPase activity and produce force in association with microtubules. These proteins - kinesin, cytoplasmic dynein, and dynamin - are referred to as cytoplasmic motors. The latter two will be the subject of this talk.Cytoplasmic dynein was first identified as one of the high molecular weight brain MAPs, MAP 1C. It was determined to be structurally equivalent to ciliary and flagellar dynein, and to produce force toward the minus ends of microtubules, opposite to kinesin.

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