DETERMINATION OF LABORATORY SPECIFIC PERCENT NORMAL SPERM MORPHOLOGY VALUE BASED ON PREVASECTOMY ("FERTILE") EJACULATES

BACKGROUND Semen analysis is an integral part of work up for infertility in men, with sperm morphology being an important qualitative parameter. Qualitative defects can affect any part of the sperm and are classified as defects in the head, middle piece, and tail, based on morphology. The focus of the study was to assess qualitative defects in sperms by light microscopy, in semen with normal sperm counts. METHODS This study is hospital based, descriptive, retrospective study. Of the semen samples received in the clinical laboratory, fifty with normal sperm counts were included in the study and processed according to standard protocol. For evaluation of qualitative defects by sperm morphology, smears were fixed in ethanol, stained with Papanicolaou stain [PAP], and assessed under light microscope. RESULTS The 50 semen samples included in the study had sperm counts ranging from 15 to 80 million / ml. Thirty samples had less than 10 % abnormal forms, fourteen samples had 11 - 20 % abnormal forms, five samples had 21 - 30 % abnormal forms and one sample had 40 % abnormal sperms. Qualitative defects were classified as morphological abnormalities in head, neck, and tail. Of the fifty cases, most defects were found in the head, followed by those in the neck and tail. Common defects noted were double head (44 %), abnormal sized heads, and bent neck (48 %). Coiling was a common defect noted in the tail (10 %). Most sperms showed a combination of defects. CONCLUSIONS Qualitative defects in sperm morphology are often seen in samples with normal sperm counts. Assessment of microscopic characteristics of human spermatozoa is as important as count and motility in the complete evaluation and work-up of semen samples in cases of infertility. KEY WORDS Semen, Sperm, Quality, Microscopy, Morphology

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Male traits and herd reproductive capability in tropical beef cattle. 2. Genetic parameters of bull traits

Animal Production Science ◽

10.1071/an12163 ◽

2013 ◽

Vol 53 (2) ◽

pp. 101 ◽

Cited By ~ 32

Author(s):

N. J. Corbet ◽

B. M. Burns ◽

D. J. Johnston ◽

M. L. Wolcott ◽

D. H. Corbet ◽

...

Keyword(s):

Body Condition ◽

Semen Quality ◽

Body Condition Score ◽

Progressive Motility ◽

Scrotal Circumference ◽

Normal Sperm ◽

Igf I ◽

Condition Score ◽

Mass Activity ◽

Percent Normal

A total of 4063 young bulls of two tropical genotypes (1639 Brahman and 2424 Tropical Composite) raised in northern Australia were evaluated for a comprehensive range of production and reproduction traits up to 24 months of age. Prior to weaning, peripheral blood concentrations of luteinising hormone (LH) and inhibin were measured at 4 months of age. At weaning (6 months) blood insulin-like growth factor-1 (IGF-I) and flight time were recorded. Body composition traits of fat depth and eye-muscle area were determined by ultrasonography at 15 months of age when additional measurements of liveweight, hip height and body condition score were recorded. Bull breeding soundness was evaluated at ~12, 18 and 24 months of age when measurements of scrotal circumference, sheath score, semen mass activity, progressive motility of individual sperm and percent morphologically normal sperm were recorded. Magnitude of heritability and genetic correlations changed across time for some traits. Heritability of LH, inhibin, IGF-I and of 18-month scrotal circumference, mass activity, progressive motility and percent normal sperm was 0.31, 0.74, 0.44, 0.75, 0.24, 0.15 and 0.25, respectively, for Brahmans and 0.48, 0.72, 0.36, 0.43, 0.13, 0.15 and 0.20, respectively, for Tropical Composites. Inhibin and IGF-I had moderate genetic association with percent normal sperm at 24 months in Brahmans but low to negligible associations in Tropical Composites. Body condition score in Brahmans and sperm motility (mass and individual) traits in both genotypes had moderate to strong genetic correlation with percent normal sperm and may prove useful candidates for indirect selection. There is scope to increase scrotal circumference by selection and this will be associated with favourable correlated responses of improved semen quality in both genotypes. The lack of genetic antagonism among bull traits indicates that selection for improved semen quality will not adversely affect other production traits.

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Normal sperm morphology and chromatin packaging: comparison between aniline blue and chromomycin A3 staining

Andrologia ◽

10.1046/j.1439-0272.1999.00290.x ◽

1999 ◽

Vol 31 (6) ◽

pp. 361-366 ◽

Cited By ~ 69

Author(s):

D. R. Franken ◽

C. J. Franken ◽

H. de la Guerre ◽

A. de Villiers

Keyword(s):

Sperm Morphology ◽

Aniline Blue ◽

Chromomycin A3 ◽

Normal Sperm

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R-027. Normal sperm morphology predicts the outcome of IUI

Human Reproduction ◽

10.1093/humrep/12.suppl_2.248-a ◽

1997 ◽

Vol 12 (Suppl_2) ◽

pp. 248-249

Author(s):

C. Keck ◽

C. Gerber-Schafer ◽

J. Neulen ◽

N. Breckwoldt

Keyword(s):

Sperm Morphology ◽

Normal Sperm

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Effect of normal sperm morphology rate (NSMR) on clinical outcomes and fertilization methods selection in the ultra-short-term GnRH-a protocol

Gynecological Endocrinology ◽

10.3109/09513590.2015.1092514 ◽

2015 ◽

Vol 32 (2) ◽

pp. 120-123 ◽

Cited By ~ 3

Author(s):

Mingzhao Li ◽

Chun Ma ◽

Xia Xue ◽

Wanqiu Zhao ◽

Silin Zhang ◽

...

Keyword(s):

Clinical Outcomes ◽

Sperm Morphology ◽

Short Term ◽

Normal Sperm

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The employment of IVF techniques for establishment of sodium, copper and selenium impact upon human sperm quality

Reproduction Fertility and Development ◽

10.1071/rd15041 ◽

2016 ◽

Vol 28 (10) ◽

pp. 1518 ◽

Cited By ~ 4

Author(s):

Urszula Marzec-Wróblewska ◽

Piotr Kamiński ◽

Paweł Łakota ◽

Marek Szymański ◽

Karolina Wasilow ◽

...

Keyword(s):

Sperm Motility ◽

Sperm Morphology ◽

Sperm Quality ◽

Sperm Concentration ◽

Human Sperm ◽

Human Semen ◽

Glutathione Peroxidase Activity ◽

Normal Sperm ◽

Group I ◽

Gpx Activity

We analysed sodium (Na), copper (Cu) and selenium (Se) levels in human semen and glutathione peroxidase activity (GPx) in seminal plasma and examined their relationships with sperm quality. Semen samples were obtained from men (n = 168) undergoing routine infertility evaluation. The study design included two groups based on standard ejaculate parameters: Group I (n = 39) with normal ejaculates (normozoospermia) and Group II (n = 129) with a pathological spermiogram. Se concentration (but not Na or Cu) and GPx activity were significantly higher in normozoospermic males than in those with a pathological spermiogram and also in males with correct sperm motility and normal sperm morphology than in asthenozoospermic and teratozoospermic males. There were significant correlations between sperm motility, Se and GPx, between rapid progressive motility and Cu, between sperm motility and Na, between normal sperm morphology and Se and Cu and between sperm concentration and Cu and GPx. Significant correlations were found between Na and Cu, between Na and Se and between Cu and Se in human semen in relation to alcohol consumption and tobacco use. Na, Cu, Se and GPx are related to sperm characteristics and male fertility and their survey could improve male infertility diagnosis.

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Observations on spermatogenesis, sperm phenotype, and fertility in white-tailed × mule deer hybrids and a yak × cow hybrid

Canadian Journal of Zoology ◽

10.1139/z88-240 ◽

1988 ◽

Vol 66 (7) ◽

pp. 1664-1671 ◽

Cited By ~ 8

Author(s):

William D. Wishart ◽

Francis Hrudka ◽

Sheila M. Schmutz ◽

Peter F. Flood

Keyword(s):

Sperm Morphology ◽

Parental Species ◽

Mule Deer ◽

Odocoileus Hemionus ◽

Species Interaction ◽

The Other ◽

Sperm Production ◽

Histological Techniques ◽

Normal Sperm ◽

Testicular Differentiation

Fertility, spermatogenesis, and sperm phenotype were investigated in three hybrid deer (Odocoileus virginianus dacotensis × Odocoileus hemionus hemionus) and a yak × domestic cow hybrid (Bos mutus (grunniens) × Bos primigenius (taurus)) using histological techniques. All of the hybrids studied were infertile but varied in the degree of testicular differentiation, spermatogenic activity, and sperm production. The hybrid yak was the least developed and the white-tailed deer × F1 hybrid was the most advanced. F1 backcrossing improved spermatogenesis, output, and morphology of sperm, but not the fertility of the donor, indicating that normal sperm morphology alone does not assure fertility. Two deer hybrids that produced sperm differed in sperm phenotype from each other and from the parental species. Interaction of a Y chromosome from one species and autosomal sex-determining genes from the other species is suggested as a possible explanation of sterility in male hybrids.

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Comparative Study of Abnormalities of the Spermocytogram between Manual Techniques versus Automated SCA (Sperm Class Analyzer)

INTERNATIONAL JOURNAL OF MULTIDISCIPLINARY RESEARCH AND ANALYSIS ◽

10.47191/ijmra/v4-i12-31 ◽

2021 ◽

Vol 04 (12) ◽

Author(s):

Zahra Ait Yachou ◽

Keyword(s):

Comparative Study ◽

Sperm Morphology ◽

Diagnostic Value ◽

Human Semen ◽

Morphological Evaluation ◽

Sperm Abnormalities ◽

Normal Sperm ◽

Abnormal Sperm

It is already well established that the percentage of normal sperm and specific sperm abnormalities has diagnostic value in vivo. The spermocytogram is an important part of the analysis of human semen, this kind of analysis, simple at first sight, brings real difficulties because the results from one laboratory to another are very relatively reliable. When analyzing abnormal sperm morphology, the observer has to estimate the respective size of the different spermatozoa or their components, the length of all the spermatozoa (too small or too big) and to identify the shape (sperm with a coiled flagellum, or no flagellum as an example). The objective of our work is first of all to make a comparative study of the results of the morphological evaluation of 50 semen samples between the manual technique versus the automated one (SCA) in order to find a possible correlation between these two techniques. According to the analytical stage of the manual and automatic results, the two latter led us to obtain significantly different percentages.

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PENGARUH JAMU DENGAN Tribulus terrestris TERHADAP KUALITAS SPERMA TIKUS WISTAR JANTAN (Rattus norvegicus)

Jurnal e-Biomedik ◽

10.35790/ebm.3.2.2015.8861 ◽

2015 ◽

Vol 3 (2) ◽

Author(s):

Delano Pelealu ◽

Lydia Tendean ◽

Benny Wantouw

Keyword(s):

Experimental Study ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Sperm Morphology ◽

Sperm Quality ◽

Control Group ◽

Tribulus Terrestris ◽

Normal Morphology ◽

Normal Sperm ◽

Male Wistar Rats

Abstract: It is known that Tribulus terrestris can improve sperm quality. One of the Indonesian traditional medicine, jamu, contains this Tribulus terrestris. This study aimed to investigate the effect of jamu containing Tribulus terrestris on sperm quality which consisted of concentration, motility, and morphology of spermatozoa of male wistar rats (Rattus norvegicus). This was an experimental study. Nine male wistar rats were randomly divided into 3 groups of 3 rats each: Group P0 (control), group P1 (given 5 mg of Tribulus terrestris), and group P2 (given 10 mg of Tribulus terrestris). This study was conducted for 52 days. The results showed that the spermatozoa concentration of P1 was 49x106 sperm/ml suspension and of P2 53x106 sperm/ml suspension; both were lower than of P0 (59x106 sperm/ml suspension). The sperm motility category (A+B) of P1 was 33% and of P2 (37%); both were lower than of P0 (45%). The motility category A was not found in P1 and P2. The percentage of spermatozoa with normal morphology of P1 was 45% and the abnormal ones was 55%, meanwhile in P2 there was 49% of normal sperm morphology and 51% of abnormal morphology. Conclusion: Jamu containing Tribulus terrestris had no effect in improvement of the sperm quality.Keywords: jamu, Tribulus terrestris, sperm qualityAbstrak: Tribulus terrestris dikenal sebagai bahan yang dapat memperbaiki kualitas sperma. Salah satu jenis jamu yang diproduksi di Indonesia mengandung Tribulus terrestris Penelitian ini bertujuan untuk mengetahui pengaruh jamu dengan Tribulus terrestris terhadap konsentrasi, motilitas, dan morfologi spermatozoa tikus wistar jantan (Rattus norvegicus). Penelitian ini menggunakan metode eksperimental. Sampel 9 ekor tikus wistar jantan (Rattus norvegicus) dibagi menjadi 3 kelompok yakni, kelompok P0 (kontrol); kelompok P1 (pemberian Tribulus terrestris 5 mg); dan kelompok P2 (pemberian Tribulus terrestris 10 mg). Penelitian dilakukan selama 52 hari. Hasil penelitian menunjukkan bahwa konsentrasi spermatozoa P1 49x106 sperma/ml suspensi dan P2 53x106 sperma/ml suspensi lebih rendah dibandingkan P0 59x106 sperma/ml suspensi. Motilitas spermatozoa kategori A+B pada P1 (33%) dan P2 (37%) lebih rendah dibandingkan pada P0 (45%). Tidak ditemukan motilitas kategori A pada P1 dan P2. Morfologi spermatozoa normal pada P1 sebesar 45% dan abnormal 55%. P2 memiliki morfologi spermatozoa normal sebesar 49% dan abnormal 51%. Simpulan: Pemberian jamu dengan Tribulus terrestris tidak meningkatkan kualitas sperma yang mencakup konsentrasi, motilitas, dan morfologi spermatozoa.Kata kunci: jamu, Tribulus terrestris, kualitas sperma

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