scholarly journals Comparative Study of Abnormalities of the Spermocytogram between Manual Techniques versus Automated SCA (Sperm Class Analyzer)

2021 ◽  
Vol 04 (12) ◽  
Author(s):  
Zahra Ait Yachou ◽  
Keyword(s):  
Comparative Study ◽  
Sperm Morphology ◽  
Diagnostic Value ◽  
Human Semen ◽  
Morphological Evaluation ◽  
Sperm Abnormalities ◽  
Normal Sperm ◽  
Abnormal Sperm

It is already well established that the percentage of normal sperm and specific sperm abnormalities has diagnostic value in vivo. The spermocytogram is an important part of the analysis of human semen, this kind of analysis, simple at first sight, brings real difficulties because the results from one laboratory to another are very relatively reliable. When analyzing abnormal sperm morphology, the observer has to estimate the respective size of the different spermatozoa or their components, the length of all the spermatozoa (too small or too big) and to identify the shape (sperm with a coiled flagellum, or no flagellum as an example). The objective of our work is first of all to make a comparative study of the results of the morphological evaluation of 50 semen samples between the manual technique versus the automated one (SCA) in order to find a possible correlation between these two techniques. According to the analytical stage of the manual and automatic results, the two latter led us to obtain significantly different percentages.

The employment of IVF techniques for establishment of sodium, copper and selenium impact upon human sperm quality

2016 ◽  
Vol 28 (10) ◽  
pp. 1518 ◽  
Author(s):  
Urszula Marzec-Wróblewska ◽  
Piotr Kamiński ◽  
Paweł Łakota ◽  
Marek Szymański ◽  
Karolina Wasilow ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Morphology ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Human Sperm ◽  
Human Semen ◽  
Glutathione Peroxidase Activity ◽  
Normal Sperm ◽  
Group I ◽  
Gpx Activity

We analysed sodium (Na), copper (Cu) and selenium (Se) levels in human semen and glutathione peroxidase activity (GPx) in seminal plasma and examined their relationships with sperm quality. Semen samples were obtained from men (n = 168) undergoing routine infertility evaluation. The study design included two groups based on standard ejaculate parameters: Group I (n = 39) with normal ejaculates (normozoospermia) and Group II (n = 129) with a pathological spermiogram. Se concentration (but not Na or Cu) and GPx activity were significantly higher in normozoospermic males than in those with a pathological spermiogram and also in males with correct sperm motility and normal sperm morphology than in asthenozoospermic and teratozoospermic males. There were significant correlations between sperm motility, Se and GPx, between rapid progressive motility and Cu, between sperm motility and Na, between normal sperm morphology and Se and Cu and between sperm concentration and Cu and GPx. Significant correlations were found between Na and Cu, between Na and Se and between Cu and Se in human semen in relation to alcohol consumption and tobacco use. Na, Cu, Se and GPx are related to sperm characteristics and male fertility and their survey could improve male infertility diagnosis.

scholarly journals Correlation between age, testosterone and adiponectin concentrations, and sperm abnormalities in Simmental bulls

2021 ◽  
pp. 2124-2130
Author(s):  
Abdullah Baharun ◽  
Syahruddin Said ◽  
Raden Iis Arifiantini ◽  
Ni Wayan K. Karja
Keyword(s):  
Sperm Morphology ◽  
Study Data ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Adiponectin Concentration ◽  
Testosterone Concentration ◽  
Carbol Fuchsin ◽  
Sperm Abnormalities ◽  
Abnormal Sperm ◽  
The Relationship

Background and Aim: Capacity for sperm production is affected by age, which is related to the morphology of sperm abnormalities and can affect fertility. The aim of this study was to evaluate the relationship between age and concentrations of testosterone and adiponectin with sperm abnormalities in Simmental bulls. Materials and Methods: The study used 11 bulls, separated into three groups. The first group consisted of five bulls aged 4-5 years, and the second and third groups each consisted of three bulls, aged 6-7 and 8-10 years, respectively. The average sperm motility of the animals ranged from 57.66±2.60% to 70.17±0.22%. Blood samples were obtained from the coccygeal region of the animals. Testosterone and adiponectin analysis was performed using the enzyme-linked immunosorbent assay method. Sperm morphology was evaluated using carbol fuchsin-eosin staining according to the Williams method. Finally, correlations between testosterone and adiponectin concentrations, age, and sperm abnormalities were analyzed using Pearson's correlation analysis. Results: The findings revealed a significant correlation (p<0.01) between the concentrations of testosterone and adiponectin (–0.538), age (–0.588), and abnormal sperm morphology (–0.912). Moreover, they revealed that the concentration of testosterone in the bulls aged 8-10 years was lower, at 21.89±4.56 ng/mL, compared to that in the bulls aged 4-5 years, at 36.15±1.29 ng/mL, and 6-7 years, at 35.16±5.39 ng/mL. The findings also revealed a positive correlation between adiponectin concentration and age (0.529) and sperm abnormalities (0.506). The increase in testosterone concentration was inversely related to the adiponectin concentration (–0.538). Moreover, the mean amount of abnormal sperm increased with increasing age: 3.82±0.33% in the group aged 4-5 years, and 4.40±0.72% and 10.20±1.97% in the groups aged 6-7 years and 8-10 years, respectively. Conclusion: The study data indicate that there is a decrease in testosterone concentration, a high adiponectin concentration, and an increase in abnormal sperm with increasing age in bulls.

scholarly journals Abnormal sperm morphology in mouse germ cells after short-term exposures to acetamiprid, propineb, and their mixture

2014 ◽  
Vol 65 (1) ◽  
pp. 47-56 ◽  
Author(s):  
Pinar Göç Rasgele
Keyword(s):  
Germ Cells ◽  
Sperm Morphology ◽  
Molecular Level ◽  
Fungal Infections ◽  
Genotoxic Effects ◽  
Short Term ◽  
Sperm Abnormalities ◽  
Abnormal Sperm ◽  
Neonicotinoid Insecticide ◽  
Insight Into

Summary Pesticides are one of the most potent environmental contaminants, which accumulate in biotic and abiotic components of ecosystems. Acetamiprid (Acm), a neonicotinoid insecticide, and Propineb (Pro), a dithiocarbamate fungicide, are widely used to control sucking insects and fungal infections on crops, respectively. The present study was undertaken to investigate the genotoxic effects of these compounds, individually and in mixtures, in mouse germ cells by using the sperm morphology assay. Mice were injected intraperitoneally with 0.625, 1.25, and 2.50 μg mL-1 of Acm, 12.5, 25, and 50 μg mL-1 of Pro, and their mixture at the same concentrations over 24 and 48 h. Acm did not significantly increase the percentage of abnormal sperm at any concentration. The frequency of abnormal sperm significantly increased after 24 and 48 h of exposure to 50 μg mL-1 of Pro. The mixtures of 2.50 μg mL-1 of Acm and 50 μg mL-1 of Pro induced sperm abnormalities antagonistically both after 24 and 48 h of exposure. Results suggest that Acm was non-genotoxic for mouse germ cells, while Pro may have been a germ cell mutagen due to the observed increase in the frequency of sperm abnormalities. However, to gain better insight into the mutagenicity and DNA damaging potential of both of these pesticides, further studies at molecular level should be done.

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