A validated LC–MS/MS method for fast detection of thiodiglycolic acid in aqueous samples

Author(s):  
Igor Rodin ◽  
Arcady Braun ◽  
Andrey Stavrianidi ◽  
Timur Baygildiev ◽  
Igor Rybalchenko ◽  
...  
Author(s):  
Jia Lu ◽  
Xiaohou Shao ◽  
Chao Yin ◽  
Xinyu Mao ◽  
Long Wang ◽  
...  

2004 ◽  
Vol 4 (2) ◽  
pp. 103-106
Author(s):  
R. Santos ◽  
S. Gonçalves ◽  
F. Macieira ◽  
F. Oliveira ◽  
R. Rodrigues ◽  
...  

In recent years, non-tuberculous mycobacteria (NTM), once considered merely environmental saprophytes, have emerged as a major cause of opportunistic infections. There is no evidence of human-to-human transmission but they have been found in several environmental water samples. It is, therefore, of the utmost importance to develop methods of rapidly and accurately detecting non-tuberculous mycobacteria in water samples. To obtain a maximum recovery rate and a reduction of Mycobacterium spp. detection time in water samples, different decontamination, enrichment procedures and antibiotics supplements were tested before the inoculation into the Bactec® system. The proposed method of sample treatment (decrease in the decontamination time, followed for a peptone pre-enrichment step and an aztreonam and cefepime supplement) before the inoculation into the Bactec® system proved to be a good option for reliable and fast detection of Mycobacterium spp. in water samples.


2019 ◽  
Vol 26 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Jen Bohon

Background: First developed in the 1990’s at the National Synchrotron Light Source, xray synchrotron footprinting is an ideal technique for the analysis of solution-state structure and dynamics of macromolecules. Hydroxyl radicals generated in aqueous samples by intense x-ray beams serve as fine probes of solvent accessibility, rapidly and irreversibly reacting with solvent exposed residues to provide a “snapshot” of the sample state at the time of exposure. Over the last few decades, improvements in instrumentation to expand the technology have continuously pushed the boundaries of biological systems that can be studied using the technique. Conclusion: Dedicated synchrotron beamlines provide important resources for examining fundamental biological mechanisms of folding, ligand binding, catalysis, transcription, translation, and macromolecular assembly. The legacy of synchrotron footprinting at NSLS has led to significant improvement in our understanding of many biological systems, from identifying key structural components in enzymes and transporters to in vivo studies of ribosome assembly. This work continues at the XFP (17-BM) beamline at NSLS-II and facilities at ALS, which are currently accepting proposals for use.


2010 ◽  
Vol 75 (5) ◽  
pp. 563-575 ◽  
Author(s):  
Moslem Mohammadi ◽  
Mehdi Khodadadian ◽  
Mohammad K. Rofouei

A plasticized poly(vinyl chloride) membrane electrode based on 4-[(5-mercapto-1,3,4-thiadiazol-2-ylimino)methyl]benzene-1,3-diol (L) for highly selective determination of palladium(II) (in PdCl42– form) is developed. The electrode showed a good Nernstian response (29.6 ± 0.4 mV per decade) over a wide concentration range (3.1 × 10–7 to 1.0 × 10–2 mol l–1). The limit of detection was 1.5 × 10–7 mol l–1. The electrode has a response time of about 20 s, and it can be used for at least 2 months without observing any considerable deviation from Nernstian response. The proposed electrode could be used in the pH range of 2.5–5.5. The practical utility of the electrode has been demonstrated by its use for the estimation of palladium content in aqueous samples.


2010 ◽  
Vol 24 (7) ◽  
pp. 2065-2075 ◽  
Author(s):  
E. Zhang ◽  
J. Antoni ◽  
R. Pintelon ◽  
J. Schoukens

2021 ◽  
Vol 11 (14) ◽  
pp. 6269
Author(s):  
Wang Jing ◽  
Wang Leqi ◽  
Han Yanling ◽  
Zhang Yun ◽  
Zhou Ruyan

For the fast detection and recognition of apple fruit targets, based on the real-time DeepSnake deep learning instance segmentation model, this paper provided an algorithm basis for the practical application and promotion of apple picking robots. Since the initial detection results have an important impact on the subsequent edge prediction, this paper proposed an automatic detection method for apple fruit targets in natural environments based on saliency detection and traditional color difference methods. Combined with the original image, the histogram backprojection algorithm was used to further optimize the salient image results. A dynamic adaptive overlapping target separation algorithm was proposed to locate the single target fruit and further to determine the initial contour for DeepSnake, in view of the possible overlapping fruit regions in the saliency map. Finally, the target fruit was labeled based on the segmentation results of the examples. In the experiment, 300 training datasets were used to train the DeepSnake model, and the self-built dataset containing 1036 pictures of apples in various situations under natural environment was tested. The detection accuracy of target fruits under non-overlapping shaded fruits, overlapping fruits, shaded branches and leaves, and poor illumination conditions were 99.12%, 94.78%, 90.71%, and 94.46% respectively. The comprehensive detection accuracy was 95.66%, and the average processing time was 0.42 s in 1036 test images, which showed that the proposed algorithm can effectively separate the overlapping fruits through a not-very-large training samples and realize the rapid and accurate detection of apple targets.


2021 ◽  
pp. 418-424
Author(s):  
Aisha Al Busaidi ◽  
Ahmed Al-Hinai

We experienced an atypical endophthalmitis occurring post consecutively performed in-office procedures; an intravitreal injection (IVI) of ranibizumab followed by an anterior chamber (AC) paracentesis performed twice in an eye with neovascular glaucoma (NVG). A 52-year-old diabetic male who was asymptomatic developed signs of endophthalmitis and decreased vision without pain in his left eye a few days post-IVI and AC paracentesis. The condition worsened after an initial vitreous tap and injection of antibiotics. Cultures of vitreous and aqueous samples were negative. Complete resolution occurred after a pars plana vitrectomy with IVI of antibiotics and steroid with removal of a dense “yellowish-brown” fibrinous plaque. The absence of pain, presence of a peculiar colored fibrin, mild-to-moderate vitritis without retinitis, negative cultures, and complete recovery despite the fulminant presentation; favor a diagnosis of inflammation over infection. We hypothesize that a micro-leak from a 26-gauge AC tap tract might have served as an entry port for 5% povidone-iodine from the ocular surface thus inciting inflammation. However, an exuberant inflammatory response that can be typically seen in NVG eyes after intraocular procedures cannot be excluded. Various causes of inflammation post-procedures, both toxic and nontoxic should be considered in atypical culture-negative fulminant endophthalmitis cases with good outcome posttreatment. Any minor ocular procedure may carry a risk of such complication. Patient counseling and care must be exercised in performing these procedures.


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