Optimisation of a cost-effective culture medium for the large-scale production ofBacillus subtilisUTB96

2013 ◽  
Vol 46 (13) ◽  
pp. 1552-1563 ◽  
Author(s):  
Soleiman Ghasemi ◽  
Masoud Ahmadzadeh
Keyword(s):  
Large Scale ◽  
Culture Medium ◽  
Cost Effective ◽  
Scale Production ◽  
Large Scale Production

scholarly journals Adaptation of Mycobacterium smegmatis to an Industrial Scale Medium and Isolation of the Mycobacterial PorinMspA

2013 ◽  
Vol 7 (1) ◽  
pp. 92-98 ◽  
Author(s):  
Sebastian O Wendel ◽  
Ayomi S Perera ◽  
Peter H Pfromm ◽  
Peter Czermak ◽  
Stefan H Bossmann
Keyword(s):  
Growth Medium ◽  
Mycobacterium Smegmatis ◽  
Large Scale ◽  
Culture Medium ◽  
Cell Mass ◽  
Cost Effective ◽  
Scale Production ◽  
Large Scale Production ◽  
Simple Growth ◽  
Effective Growth

The adaptation of the organism to a simple and cost-effective growth medium is mandatory in developing a process for large scale production of the octamericporinMspA, which is isolated fromMycobacterium smegmatis. A fermentation optimization with the minimal nutrients required for growth has been performed. During the fermentation, the iron- and ammonium chloride concentrations in the medium were varied to determine their impact on the observed growth rates and cell mass yields. Common antibiotics to control contamination were eliminated in favor of copper sulfate to reduce costs. MspA has been successfully isolated from the harvestedM. smegmatisusingaqueous nOPOE (n-octyloligooxyethylene) at 65°C. Because of the extraordinary stability of MspA, it is possible to denature and precipitate virtually all other proteins and contaminants by following this approach. To further purify the product, acetone is used for precipitation. Gel electrophoresis confirmed the presence and purity of MspA. A maximum of 840µg (via Bradford assay) of pure MspA per liter of the optimized simple growth medium has been obtained. This is a 40% increase with respect to the previously reported culture medium for MspA.

scholarly journals Transgenic goats producing an improved version of cetuximab in milk

2020 ◽  
Author(s):  
Götz Laible ◽  
Sally Cole ◽  
Brigid Brophy ◽  
Paul Maclean ◽  
Li How Chen ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Large Scale ◽  
Growth Factor Receptor ◽  
Cost Effective ◽  
Scale Production ◽  
Cancer Treatments ◽  
Large Scale Production ◽  
Immunogenic Epitope ◽  
Dependent Cell ◽  
Transgenic Goats

ABSTRACTTherapeutic monoclonal antibodies (mAbs) represent one of the most important classes of pharmaceutical proteins to treat human diseases. Most are produced in cultured mammalian cells which is expensive, limiting their availability. Goats, striking a good balance between a relatively short generation time and copious milk yield, present an alternative platform for the cost-effective, flexible, large-scale production of therapeutic mAbs. Here, we focused on cetuximab, a mAb against epidermal growth factor receptor, that is commercially produced under the brand name Erbitux and approved for anti-cancer treatments. We generated several transgenic goat lines that produce cetuximab in their milk. Two lines were selected for detailed characterization. Both showed stable genotypes and cetuximab production levels of up to 10g/L. The mAb could be readily purified and showed improved characteristics compared to Erbitux. The goat-produced cetuximab (gCetuximab) lacked a highly immunogenic epitope that is part of Erbitux. Moreover, it showed enhanced binding to CD16 and increased antibody-dependent cell-dependent cytotoxicity compared to Erbitux. This indicates that these goats produce an improved cetuximab version with the potential for enhanced effectiveness and better safety profile compared to treatments with Erbitux. In addition, our study validates transgenic goats as an excellent platform for large-scale production of therapeutic mAbs.

ChipsetT: Embedded Die Substrate Applications

2013 ◽  
Vol 2013 (DPC) ◽  
pp. 000377-000397
Author(s):  
Jon G. Aday ◽  
Ted Tessier ◽  
Kazuhisa Itoi ◽  
Satoshi Okude
Keyword(s):  
Large Scale ◽  
Cost Effective ◽  
Scale Production ◽  
Robust Solution ◽  
Sintered Metal ◽  
Design Flexibility ◽  
Fine Pitch ◽  
Large Scale Production ◽  
Bottom Side ◽  
Board Level

Embedded die substrate technologies are being developed in an assortment of configurations and for different market segments. The technology being discussed in this paper will be focused on both a fan out technology – ChipsetT Fan-Out and a system in package approach (ChipsetT SiP) in which a multiple component bill of materials (BOM) is used. The Chipset process is based on the WABE (Wafer and Board Level Embedding) technology. WABE technology is based on co-lamination of multilayer polyimide flex wiring and conductive z-axis sintered metal interconnections. This ChipsetT Fan Out technology allows for large scale production of fan out type solutions which can allow for very thin packages in addition to unique pin out solutions such as pin compatibility for a competitor part. The ChipsetT SiP also allows embedding of single or multiple silicon die and/or components. Additional components can also be placed using conventional SMT on the top or bottom side of the package. There is a great deal of design flexibility with this technology which makes it a great solution for applications trying to reduce their x-y size or z-height. When utilizing RDL technology on the embedded die we are able to do the fine pitch routing in order to allow the substrate to route at larger pitches ensuring an overall cost effective solution. This paper will focus on the different classes of applications that have benefited from this technology and will discuss the benefits and tradeoffs of the different solutions that have been engineered. Assembly and reliability data will be presented on several of the applications showing a robust solution set.

Electro-reduction of hematite using water as the redox mediator

2019 ◽  
Vol 21 (2) ◽  
pp. 198-204 ◽  
Author(s):  
Kaiyu Xie ◽  
Ali Reza Kamali
Keyword(s):  
Molten Salt ◽  
Large Scale ◽  
Cost Effective ◽  
Redox Mediator ◽  
Electrolytic Reduction ◽  
Scale Production ◽  
Large Scale Production ◽  
Cost Effective Approach

Molten salt electrolytic reduction of Fe2O3 in the presence of water is proposed as a sustainable and cost-effective approach for large-scale production of iron.

Aluminium and Aluminium Alloy Powders for P/M Applications

2007 ◽  
Vol 534-536 ◽  
pp. 773-776
Author(s):  
R. Cook ◽  
I.T.H. Chang ◽  
C. Lucien Falticeanu
Keyword(s):  
Particle Size ◽  
Aluminium Alloy ◽  
Large Scale ◽  
Cost Effective ◽  
Powder Particle Size ◽  
Scale Production ◽  
Matrix Composites ◽  
Weight Saving ◽  
Large Scale Production ◽  
Particle Size And Structure

P/M aluminium components are attracting interest in an increasing variety of industries due to the possibilities for weight saving in engineering parts. There are many processes for manufacturing from powder feedstocks that are either in production, becoming commercialised or still undergoing development. The nature of these processes and the required properties of the end products mean that powders of different particle size, shape, composition and microstructure must be produced. The requirements of various processes requiring aluminium and aluminium alloy powders for metal matrix composites, laser sintering, powder forging and metal injection moulding are discussed in relation to powder particle size and structure. The key requirement of the powder manufacturer is to supply cost effective materials for these different processes. This may require compromises to be made by the supplier and consumer while the techniques evolve from development to large scale production.

scholarly journals Biochemical and morpho-anatomical analyses of strawberry vitroplants hyperhydric tissues affected by BA and gelling agents

Revista CERES ◽  
2013 ◽  
Vol 60 (2) ◽  
pp. 152-160 ◽  
Author(s):  
Leticia Mascarenhas Pereira Barbosa ◽  
Vespasiano Borges de Paiva Neto ◽  
Leonardo Lucas Carnevalli Dias ◽  
Reginaldo Alves Festucci-Buselli ◽  
Rodrigo Sobreira Alexandre ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Large Scale ◽  
Culture Medium ◽  
Shoot Proliferation ◽  
Cellular Level ◽  
Fragaria X Ananassa ◽  
Scale Production ◽  
Ms Medium ◽  
Large Scale Production

In vitro propagation has become an effective practice for large-scale production of strawberry plants. The objective of this study was to evaluate the hyperhydricity and the multiplication capacity of two strawberry varieties (Fragaria x ananassa Duch. 'Dover' and 'Burkley') propagated in vitro. Plants maintained in MS medium supplemented with 1.0 mg L-1 BA were individualized and transferred to the same medium solidified with Agar (6.5 g L-1) or Phytagel® (2.5 g L-1) and BA at different concentrations (0; 0.5; 1.0; 2.0 and 3.0 mg L-1). Biochemical and anatomical analyses were carried out, as well as the analysis of the morphological hyperhydricity characteristics. The analysis of data showed: a) the increase in cytokinin concentration increased hyperhydricity frequency in both varieties; b) at concentrations up to 2.0 mg L-1 BA, the replacement of Agar by Phytagel® induced a higher formation of hyperhydric shoots; and c) the addition of BA induced oxidative stress, which is characterized by increased antioxidant activity and lipid peroxidation, as well as alterations at the cellular level, such as malformation of stomata and epidermal cells. In conclusion, the culture medium containing 0.5 mg L-1 BA solidified with Agar provided lower hyperhydricity percentages in association with higher rates of shoot proliferation in strawberry.

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