Monitoring population levels ofPseudomonas fluorescenslabeled with green fluorescent protein, using fluorescence microscopy and direct fluorescence scanning techniques

2006 ◽  
Vol 28 (1) ◽  
pp. 125-130 ◽  
Author(s):  
R. H. Etebarian ◽  
P. L. holberg
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescence Microscopy ◽  
Fluorescent Protein ◽  
Direct Fluorescence ◽  
Green Fluorescent

scholarly journals Phagocytosed Bordetella pertussis Fails To Survive in Human Neutrophils

2000 ◽  
Vol 68 (2) ◽  
pp. 956-959 ◽  
Author(s):  
Derrick H. Lenz ◽  
Christine L. Weingart ◽  
Alison A. Weiss
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescence Microscopy ◽  
Bordetella Pertussis ◽  
Fluorescent Protein ◽  
Survival Rates ◽  
Polymyxin B ◽  
Immune Serum ◽  
Human Neutrophils ◽  
Green Fluorescent

ABSTRACT Previous studies have reported that phagocytosed Bordetella pertussis survives in human neutrophils. This issue has been reexamined. Opsonized or unopsonized bacteria expressing green fluorescent protein (GFP) were incubated with adherent human neutrophils. Phagocytosis was quantified by fluorescence microscopy, and the viability of phagocytosed bacteria was determined by colony counts following treatment with polymyxin B to kill extracellular bacteria. Only 1 to 2% of the phagocytosed bacteria remained viable. Opsonization with heat-inactivated immune serum reduced the amount of attachment and phagocytosis of the bacteria but did not alter survival rates. In contrast to previous reports, these data suggest that phagocytosed B. pertussis bacteria are killed by human neutrophils.

scholarly journals Constitutive and Inducible Expression of Green Fluorescent Protein in Brucella suis

1999 ◽  
Vol 67 (12) ◽  
pp. 6695-6697 ◽  
Author(s):  
Stephan Köhler ◽  
Safia Ouahrani-Bettache ◽  
Marion Layssac ◽  
Jacques Teyssier ◽  
Jean-Pierre Liautard
Keyword(s):  
Flow Cytometry ◽  
Green Fluorescent Protein ◽  
Fluorescence Microscopy ◽  
Gene Fusion ◽  
Fluorescent Protein ◽  
Fusion System ◽  
Chromosomal Dna ◽  
Brucella Suis ◽  
Green Fluorescent ◽  
Inducible Genes

ABSTRACT A gene fusion system based on plasmid pBBR1MCS and the expression of green fluorescent protein was developed for Brucella suis, allowing isolation of constitutive and inducible genes. Bacteria containing promoter fusions of chromosomal DNA togfp were visualized by fluorescence microscopy and examined by flow cytometry. Twelve clones containing gene fragments induced inside J774 murine macrophages were isolated and further characterized.

Use of Green Fluorescent Protein To Detect Expression of an Endopolygalacturonase Gene of Colletotrichum lindemuthianum during Bean Infection

1999 ◽  
Vol 65 (4) ◽  
pp. 1769-1771 ◽  
Author(s):  
Bernard Dumas ◽  
Sylvie Centis ◽  
Nathalie Sarrazin ◽  
Marie-Thérèse Esquerré-Tugayé
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescence Microscopy ◽  
Fluorescent Protein ◽  
Colletotrichum Lindemuthianum ◽  
Fungal Genome ◽  
Appressorium Formation ◽  
Gene Encoding ◽  
Green Fluorescent ◽  
Microscopy Examination

ABSTRACT The 5′ noncoding region of clpg2, an endopolygalacturonase gene of the bean pathogenColletotrichum lindemuthianum, was fused to the coding sequence of a gene encoding a green fluorescent protein (GFP), and the construct was introduced into the fungal genome. Detection of GFP accumulation by fluorescence microscopy examination revealed thatclpg2 was expressed at the early stages of germination of the conidia and during appressorium formation both in vitro and on the host plant.

Spectral Imaging for Separation of Fluorescent Signals from Autofluorescence

2000 ◽  
Vol 6 (S2) ◽  
pp. 838-839
Author(s):  
Richard M. Levenson
Keyword(s):  
Green Fluorescent Protein ◽  
Connective Tissue ◽  
Fluorescence Microscopy ◽  
Broad Spectrum ◽  
Fluorescent Protein ◽  
Fluorescence Signal ◽  
Post Processing ◽  
Neuronal Tissue ◽  
Green Fluorescent ◽  
Pathology Specimen

Autofluorescence , also known as adventitious fluorescence or background fluorescence, ofter poses a significant problem in many applications of fluorescence microscopy. It contributes to unwanted noise and can swamp the desired signal. Particularly difficult samples to image include many pathology specimen that have been processed using crosslinking fixatives (typically formaldehyde). This procedure dramatically increase the autofluorescence level, leading to bright, broad spectrum emissions, particularly from connective tissue components. Unprocessed plant tissue and neuronal tissue also have extremely high levels of endogenous autofluorescence that can make many convenient labeling strategies, including most (green fluorescent protein (GFP) labels, extremely problematic. Various solutions have been proposed for the reduction or elimination of autofluorescence. These include using narrow bandpass emission filters to try to isolate the desired fluorescence signal, the use of labels which can be excited at wavelengths that are much less likely to induce autofluorescence (moving the excitation towards the NIR is effective), and post-processing aldehyde-fixed samples with such reagents as sodium borohydride or toluidine blue to chemically suppress the autofluorescence signal.However, in many cases, these approaches are either infeasible or ineffective.

scholarly journals Dual‐color‐emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy

Luminescence ◽  
2013 ◽  
Vol 28 (4) ◽  
pp. 582-591 ◽  
Author(s):  
Katsunori Ogoh ◽  
Takashi Kinebuchi ◽  
Mariko Murai ◽  
Takeo Takahashi ◽  
Yoshihiro Ohmiya ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescence Microscopy ◽  
Fluorescent Protein ◽  
Ph Indicator ◽  
Dual Color ◽  
Green Fluorescent

scholarly journals Green Fluorescent Protein as a Marker in Plasmodium berghei Transformation

1999 ◽  
Vol 67 (5) ◽  
pp. 2602-2606 ◽  
Author(s):  
Ali A. Sultan ◽  
Vandana Thathy ◽  
Victor Nussenzweig ◽  
Robert Ménard
Keyword(s):  
Flow Cytometry ◽  
Green Fluorescent Protein ◽  
Plasmodium Berghei ◽  
Fluorescent Protein ◽  
Single Copy ◽  
Malarial Parasite ◽  
Molecular Studies ◽  
Direct Fluorescence ◽  
Vertebrate Hosts ◽  
Green Fluorescent

ABSTRACT We present a new marker that confers both resistance to pyrimethamine and green fluorescent protein-based fluorescence on the malarial parasite Plasmodium berghei. A single copy of the cassette integrated into the genome is sufficient to direct fluorescence in parasites throughout the life cycle, in both its mosquito and vertebrate hosts. Erythrocyte stages of the parasite that express the marker can be sorted from control parasites by flow cytometry. Pyrimethamine pressure is not necessary for maintaining the cassette in transformed parasites during their sporogonic cycle in mosquitoes, including when it is borne by a plasmid. This tool should thus prove useful in molecular studies of P. berghei, both for generating parasite variants and monitoring their behavior.

scholarly journals Influence of nanobody binding on fluorescence emission, mobility and organization of GFP-tagged proteins

2020 ◽  
Author(s):  
Falk Schneider ◽  
Christian Eggeling ◽  
Erdinc Sezgin
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescence Microscopy ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescence Emission ◽  
Fluorescence Properties ◽  
Great Care ◽  
Target Molecules ◽  
Green Fluorescent

SummaryAdvanced fluorescence microscopy studies require specific and monovalent molecular labelling with bright and photostable fluorophores. This necessity led to the widespread use of fluorescently labelled nanobodies against commonly employed fluorescent proteins. However, very little is known how these nanobodies influence their target molecules. Here, we observed clear changes of the fluorescence properties, mobility and organisation of green fluorescent protein (GFP) tagged proteins after labelling with an anti-GFP nanobody. Intriguingly, we did not observe any co-diffusion of fluorescently-labelled nanobodies with the GFP-labelled proteins. Our results suggest significant binding of the nanobodies to a non-emissive, oligomerized form of the fluorescent proteins, promoting disassembly into more monomeric forms after binding. Our findings show that great care must be taken when using nanobodies for studying dynamic and quantitative protein organisation.

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